Conformational lipids and proteins in membranes. Medical search. Frequent questions

Different aspects of the interaction between peptides from the toxins' N-termini (StI1-31 and StI12-31 SELAGTIIDGASLTFEVLDKVLGELGKVSRK, and StII1-30 and StII11-30 ALAGTIIAGASLTFQVLDKVLEELGKVSRK) and model membranes - micelles and bilayers - have been studied to contribute to the elucidation of the toxins mechanism of action at the molecular level. (bvsalud.org)
HS-AFM imaging of OmpG in lipid bilayers at pH 7.6 and pH 5.0. (nanoworld.com)
Then molecular dynamics (MD) simulations in lipid bilayers are used to pinpoint likely lipid protein interactions, which can be tested experimentally using HDX-MS. By bringing together the MD predictions with the conformational readouts from HDX-MS, we have uncovered key lipid protein interactions implicated in stabilizing important functional conformations. (uiuc.edu)
Leaflet tensions control the spatio-temporal remodeling of lipid bilayers and nanovesicles. (mpg.de)
Probing the elastic response of lipid bilayers and nanovesicles to leaflet tensions via volume per lipid. (mpg.de)
Their conformational rearrangements lead to the unification of lipid bilayers of cell membranes and viral envelopes and the formation of fusion pores through which the viral genome enters the cytoplasm of the cell. (bvsalud.org)
Headgroup Structure and Cation Binding in Phosphatidylserine Lipid Bilayers. (mpg.de)
Spatial arrangements of the porins in lipid bilayers were visualized by using the positioning of proteins in membranes web server in the Orientations of Proteins in Membranes database ( 31 ). (cdc.gov)

However, there are no data showing how exactly the regulation emerges from specific lipid-protein interactions. (helsinki.fi)
The alternative mechanism, where the β2AR conformation would be modulated by membrane-mediated interactions, plays only a minor role. (helsinki.fi)
The results highlight the capacity of lipids to regulate the conformation of membrane receptors through specific interactions. (helsinki.fi)
FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
Specific protein-lipid interactions are critical for viral assembly. (elifesciences.org)
The matrix (MA) domain drives Gag onto the plasma membrane through electrostatic interactions at its highly-basic-region (HBR), located near the myristoylated (Myr) N-terminus of the protein. (elifesciences.org)
Our realistic membrane models confirm interactions with PIP 2 and PS lipids are highly favored around the HBR, and are strong enough to keep the protein bound even without Myr insertion. (elifesciences.org)
Therefore, smFRET has been limited to studies of inter-receptor interactions in cellular membranes and receptors in detergent environments. (nature.com)
NMR structure of a protein kinase C-gamma phorbol-binding domain and study of protein-lipid micelle interactions. (embl-heidelberg.de)
This membrane anchor is attached to the protein surface by hydrophobic interactions with the protein core. (uni-goettingen.de)
The yeast PX domain-only proteins Grd19p (YOR357C) and Ypt35p (YHR105W), as well as the isolated PX domains from Mvp1p (YMR004W), Snx42p/Cvt20p/Atg20p (YDL113C), Vam7p (YGL212W), and Vps17p (YOR132W), yielded a total of 40 reproducible two-hybrid interactions. (embl.de)
Thirty-five interactions were found for the full-length proteins of Bem1p (YBR200W), Snx42p, Snx4p/Cvt13p (YJL036W), Vam7p, Vps5p (YOR069W), and Vps17p, but these appear not to require the PX domain, because these interactions could not be reproduced with PX-only baits. (embl.de)
Interactions of Grd19p, Vam7p, Vps5p, Vps17p, and Ypt35p with members of the Yip1p family of proteins were detected consistently and were verified by in vitro binding assays. (embl.de)
A mutation in the lipid-binding pocket of Ypt35p that reduces lipid binding markedly does not affect these PX domain protein interactions, arguing that lipid binding uses a different interaction surface than protein binding. (embl.de)
It has become increasingly evident that direct interactions of membrane proteins with their surrounding lipids play key roles in regulating both protein conformations and function. (uiuc.edu)
Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
Chemical cross-linking provides detailed insights into these interactions suggesting a role for membrane fusion. (nature.com)
Zippering of the SNAREs is proposed to provide the required energy to initiate membrane fusion 6 , 7 and proceeds from the N-terminus towards the membrane-proximal C-terminus through interactions of their complementary SNARE motifs. (nature.com)
These results suggest that the first transmembrane glycine residue on the β 2 subunit may be important for conformational or allosteric interactions of channel gating by both GABA and anesthetics. (aspetjournals.org)
The study of MscL, a highly convenient model system, will give us basic understanding and the first example of what type of intramolecular interactions, sequence motifs, and conformations make membrane proteins sensitive to a physiologically relevant mechanical stimulation. (usda.gov)
Prevalence and mechanism of synergistic carboxylate-cation-water interactions in halophilic proteins. (mpg.de)
Molecular interactions in cell membranes, particularly lipid-protein interactions in their hydrophobic core, are difficult to analyse and remain poorly characterised despite high relevance in physiological and pathological processes. (mappingignorance.org)
Structural rearrangements in membranes and embedded proteins, and the way molecular interactions contribute to their function are unresolved questions in biophysical chemistry. (mappingignorance.org)
The known membrane protein structures, derived mainly by crystallography, provide little insight into lipid-protein interactions as bound lipid molecules are rarely visible. (mappingignorance.org)
Our new article 1 present the proof of concept of a generalized approach for NMR studies of lipid-protein interactions that relies on sparse fluorination of the lipid acyl chains and exploits fluorine both indirectly, as shift reagent affecting the NMR signals of nearby atoms, and directly, as 19 F isotope with unique NMR properties. (mappingignorance.org)
This highly complex process is dictated by various events, such as virus motion, membrane deformation and merging as well as molecular scale protein-protein, protein- lipid interactions and drastic protein conformational changes, occurring at multiple stages and at multiple length and time scales. (upenn.edu)
Interplay of trans- and cis-interactions of glycolipids in membrane adhesion. (mpg.de)
Weak carbohydrate-carbohydrate interactions in membrane adhesion are fuzzy and generic. (mpg.de)

The structures show that both substrate translocation and release involve movements of the bulky transport domain through the lipid bilayer. (elifesciences.org)
Finally, we describe how domain movements are associated with the displacement of bound lipids and significant membrane deformations, highlighting the potential regulatory role of the bilayer. (elifesciences.org)
The two hairpins meet near the middle of the lipid bilayer, and their non-helical tips provide essential coordinating moieties for the bound L-asp. (elifesciences.org)
Because the probe, protein, and lipid bilayer are all represented in atomic detail, the different contributions to the work, such as the extent of protein backbone movements, can be separated. (uni-regensburg.de)
Association of EcPOX to the lipid bilayer is conferred by the last 23 amino acids of the C-terminal part which form an amphipathic helix in the presence of membranes. (uni-goettingen.de)
Binding to the lipid bilayer is not only mandatory for feeding the reducing equivalents into the electron transport chain, but also leads to enhancement of the catalytic activity of the enzyme. (uni-goettingen.de)
Bilayer association of the enzyme is thereby promoted by the formation of an amphipathic helix from the membrane anchor. (uni-goettingen.de)
The channel is a multimeric complex made of 15 kDa subunits and responds directly to the tension in the lipid bilayer. (usda.gov)
2016 ) Functional modulation of a GPCR conformational landscape in a lipid bilayer. (ibpc.fr)
2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)
2022 ). The YidC transmembrane (TM) groove is essential for a high- affinity interaction, and the hydrophilic nature of the YidC groove plays an important role in protein transport across the cytoplasmic membrane bilayer to the periplasmic side. (tcdb.org)
Generation of bilayer asymmetry and membrane curvature by the sugar-cleaving enzyme invertase. (mpg.de)

MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
The GFRα family consists of four members, GFRα1, GFRα2, GFRα3 and GFRα4, located roughly extracellular and anchored to the plasma membrane by glycosyl-phosphatidyl-inositol (GPI). (medsci.org)
The GFRα family consists of four members, GFRα1, GFRα2, GFRα3 and GFRα4, which are tethered to the plasma membrane through GPI anchors containing CRDs. (medsci.org)
Membrane proteins (MPs) reside in the plasma membrane and perform various biological processes including ion transport, substrate transport, and signal transduction. (nanoworld.com)
It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters. (nature.com)
Here we show that incorporation of the pore-forming toxin Cytolysin A (ClyA) into the plasma membrane of Chinese hamster ovary cells (CHO-K1) results in the appearance of single-channel conductance amenable to multiplexed automated patch-clamp (APC) electrophysiology. (bvsalud.org)

Proteins known as G protein-coupled receptors, or GPCRs for short, detect and respond to hormones and other signaling molecules found outside cells. (helsinki.fi)
G-protein coupled receptors (GPCRs) are membrane proteins that transmit the effects of extracellular ligands to effect changes in the intracellular G-protein signaling system. (gauss-centre.eu)
The complex pharmacology of G-protein-coupled receptors (GPCRs) is defined by their multi-state conformational dynamics. (nature.com)
Podosomes are structurally divided into a core, which mainly contains proteins involved in actin polymerization (such as WASP, the Arp2/3 complex and cortactin ), and a surrounding ring populated by integrin receptors and adhesion proteins (for example, paxillin and focal adhesion kinase (FAK/Pyk2) ) [15] . (cellmigration.org)
Mechanistic understanding of dynamic membrane proteins such as transporters, receptors, and channels requires accurate depictions of conformational ensembles, and the manner in which they interchange as a function of environmental factors including substrates, lipids, and inhibitors. (uni-regensburg.de)
G protein-coupled receptors (GPCRs) constitute the largest integral membrane protein family in the human genome and are involved in many important signal transduction processes. (ibpc.fr)
High-resolution X-ray crystallography will provide a structural framework of BLT receptors to understand the observed conformational changes in NMR experiments and should contribute significantly to full understanding of receptor functioning. (ibpc.fr)

Zinc ions have shown antiviral properties, but a key issue for their use for antiviral therapy is its difficulty, as a divalent metal ion, to cross the cell membrane and thus reach its targets inside the cell. (gauss-centre.eu)
If the cell contains proteins, which cannot pass through the cell membrane and there is potassium chloride, which can pass through the cell membrane, the product of potassium and chloride concentrations in the cell will be equal to the product of potassium and chloride concentrations on the outside at equilibrium. (iupac.org)
As the cell membrane is permeable to water, the only way to establish the equilibrium is by adding some ions on the outside to compensate for the high osmotic pressure inside the cell, and that ion is sodium. (iupac.org)
In the following years it was shown that cell membrane permeability to sodium was a general phenomena. (iupac.org)
By then the existence of the ion pump in the cell membrane had been accepted, but it was not known what the nature of the pump was. (iupac.org)
This added-on conduit extends T7's basic 23 nm long tail to 40-55 nm, long enough to easily bridge the periplasm and penetrate the cell membrane (CM). These ejected proteins do more than just extend the tail. (asmblog.org)
Its work finished, the tube disassembles, resealing the punctured cell membrane as it disappears. (asmblog.org)
Proteins located in the cell membrane, for example, allow for the transport of ions and small molecules, as well as communication, across the cell membrane. (kth.se)
A number of enveloped viruses confer fusion of the viral envelope and the cell membrane using surface viral fusion proteins. (bvsalud.org)

Additionally, PA can be converted into a number of other lipids, such as lysophosphatidic acid (lyso-PA) or diacylglycerol, signal molecules which have a multitude of effects on downstream cellular pathways. (wikipedia.org)
He loved microscopes and watching the proteins and molecules that make up cells move around. (biophysics.org)
Here, we performed smFRET experiments on functionally active human A 2A adenosine receptor (A 2A AR) molecules embedded in freely diffusing lipid nanodiscs to study their intramolecular conformational dynamics. (nature.com)
It is also used to investigate how lipids and small molecules may modulate allostery in membrane proteins. (kth.se)
Binding of his-tagged molecules to giant vesicles via anchor lipids. (mpg.de)
These molecules are used to label the membrane (plant, animal or liposome) in order to evaluate the membrane properties (e.g. fluidity). (ac.rs)
From the MRD data, the rotational correlation time of detergent-solubilized BR was determined to 35 ns at 300 K, con- sistent with a monomeric protein in complex with about 150 detergent molecules. (lu.se)
While the locations and inferred H-bond connec- water molecules in a membrane protein, despite tivities of these internal water molecules are essen- being deeply buried and extensively H-bonded to tial, one would also like to quantify their mobility. (lu.se)

The MARV matrix layer confers the virion filamentous shape and stability but how host lipids modulate mVP40 oligomerization is mostly unknown. (uci.edu)
Insertion events only occur with one of the membrane models, showing a combination of surface charge and internal membrane structure modulate this process. (elifesciences.org)
Biomolecular condensates modulate membrane lipid packing and hydration. (mpg.de)

One cryoEM and over 50 high-resolution X-ray crystallographic structures are available for antagonist- or agonist-bound A 2A AR and for its ternary complex with an agonist and an engineered G protein, making this receptor an excellent model system for investigating GPCR structural dynamics. (nature.com)
However, attempts to interpret DEER data are frequently stymied by discrepancies with the structural data, which may arise due to differences in conditions, the dynamics of the protein, or the flexibility of the attached paramagnetic spin labels. (uni-regensburg.de)
This work therefore illustrates how ranking simulations based on EBMetaD can help to bridge the gap between structural and biophysical data and thereby enhance our understanding of membrane protein conformational mechanisms. (uni-regensburg.de)
The 56th Annual Biophysical Society Meeting is fast approaching, and this year members of the Membrane Protein Structural Dynamics Consortium (MPSDC) have collaborated on no less than 72 presentations and posters taking place in various subgroups, platforms, symposia, mini-symposia, and workshops. (memprotein.org)
They reassemble to form the structural framework that remolds the membrane into a 50 nm long chute initially closed at its distal end. (asmblog.org)
Digital twins can make predictions that would not have been obvious from a structural model, e.g. under which conditions a membrane ruptures, or which space intrinsically disordered proteins explore. (scale-frankfurt.org)
This includes transitions between structural states, ligand binding or communication between protein domains via allostery. (kth.se)
Introduction to genomic & Proteomics, Structural genomics and proteomics, Human Genome project and other sequencing projects, Biological databases and data mining, sequence similarity search and sequence alignment, Protein structure predication and structure analysis, use of software package in Bioinformatics. (bits-pilani.ac.in)
Participates in the viral particle production as a result of its interaction with the non-structural protein 5A (By similarity). (proteopedia.org)
The first section of this review describes types of viral fusion proteins and is followed by a comparison of the structural features of class I fusion proteins, namely influenza virus hemagglutinin and the S-protein of the human coronavirus. (bvsalud.org)
Photoreceptor rhodopsin: structural and conformational study of its chromophore 11-cis retinal in oriented membranes by deuterium solid state NMR. (ox.ac.uk)
In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)

Proteins are the biomolecules within these cells which carry out different functions. (kth.se)
Small angle neutron scattering (SANS) provides unique insight into biomacromolecular complexes by combining solvent contrast variation (H2O:D2O exchange) with either natural contrast between different classes of biomolecules (proteins, RNA/DNA, lipids/detergents) and/or by applying artificial contrast, i.e. deuteration of specific biomolecules. (lu.se)

PA and its lipid derivatives are implicated in myriad processes that include intracellular vesicle trafficking, endocytosis, exocytosis, actin cytoskeleton dynamics, cell proliferation differentiation, and migration. (wikipedia.org)
This includes gene, protein and metabolic networks, cellular architecture and intracellular dynamics, cell communication and motility, cell division and differentiation, tissue formation and organogenesis, tissue and organ functions, changes in population characteristics as a consequence of interaction of organisms with their physical environment, with individuals of their own species, and with organisms of other species. (nih.gov)
We present a molecular dynamics simulation study on the binding mechanism of the membrane targeting domain of HIV-1 Gag protein. (elifesciences.org)
While static structures provide critical information about the receptor's lowest energy states, our understanding of the A 2A AR function remains critically incomplete without detailed knowledge of its conformational dynamics. (nature.com)
Integrating hydrogen-deuterium exchange mass spectrometry with molecular dynamics simulations to probe lipid-modulated conformational changes in membrane proteins. (uiuc.edu)
Measuring pico-Newton forces with lipid anchors as force sensors in molecular dynamics simulations. (mpg.de)
His current research interests mainly focus on modeling of membrane deformations, simulations of protein structures, computational fluid dynamics (CFD), and multi-scale modeling and their applications in engineering and biomedical processes. (upenn.edu)
Probing the link between pancratistatin and mitochondrial apoptosis through changes in the membrane dynamics on the nanoscale. (mpg.de)
Particle-based membrane model for mesoscopic simulation of cellular dynamics. (mpg.de)

Strictly speaking, phospholipase D is a transphosphatidylase: it mediates the exchange of polar headgroups covalently attached to membrane-bound lipids. (wikipedia.org)
The products of this hydrolysis are the membrane-bound lipid phosphatidic acid (PA), and choline, which diffuses into the cytosol. (wikipedia.org)
Crystal structures often deviate from the natural system: Proteins, especially membrane-bound ones, do not necessarily crystallize in their biologically active structures and the measures needed to obtain suitable GPCR crystals tend to increase the diversity between the natural environment and the crystal. (gauss-centre.eu)
The observed behavior indicates conformational exchange between bound and free states upon protein-micelle interaction. (embl-heidelberg.de)
The thiamine- and flavin-dependent peripheral membrane protein pyruvate oxidase from E. coli (EcPOX) catalyzes the oxidative decarboxylation of the central metabolite pyruvate to CO2 and acetate with a concomitant electron transfer step to ubiquinone 8 (Q8), a membrane-bound mobile carrier of the respiratory chain. (uni-goettingen.de)
It was shown that amino acids within the active site mediate pyruvate-induced reduction of the protein-bound flavin cofactor. (uni-goettingen.de)
Thus, the flavin acts as redox switch and a signal transfer cascade starting from the reduced flavin leads to exposure of the hitherto protein-bound membrane anchor. (uni-goettingen.de)
Similar to the stabilization at the protein the membrane anchor is also bound to the membrane by hydrophobic contacts. (uni-goettingen.de)
Mouse protein citron, a putative rho/rac effector that binds to the GTP-bound forms of rho and rac. (embl.de)
Bound phospholipids assist cholesteryl ester transfer in the cholesteryl ester transfer protein. (mpg.de)
To determine the orientation and conformation of retinal within the binding pocket of this membrane bound receptor, an ab initio solid state 2H NMR approach was used. (ox.ac.uk)

Extensive atomistic simulations show that cholesterol regulates β2AR by limiting its conformational variability. (helsinki.fi)
This protocol takes 4 d to complete, excluding the time for data analysis and MD simulations, which depends on the size of the protein under investigation. (uiuc.edu)
Such simulations can be further augmented by machine learning or be restrained with additional parameters such as membrane tension or the affinity of intrinsically disordered proteins to each other. (scale-frankfurt.org)
The topic of this thesis covers development and application of such data-driven analysis to understand molecular simulations of proteins. (kth.se)
Binding and segregation of proteins in membrane adhesion: theory, modeling, and simulations. (mpg.de)
Identifying conformational-selection and induced-fit aspects in the binding-induced folding of PMI from Markov state modeling of atomistic simulations. (mpg.de)
Protein-peptide association kinetics beyond the seconds timescale from atomistic simulations. (mpg.de)

The PX domain is approximately 120 residues long [ ( PUBMED:11373621 ) ], and folds into a three-stranded beta-sheet followed by three -helices and a proline-rich region that immediately preceeds a membrane-interaction loop and spans approximately eight hydrophobic and polar residues. (embl.de)
Rotational decoupling between the hydrophilic and hydrophobic regions in lipid membranes. (mpg.de)

PA is a negatively charged phospholipid, whose small head group promotes membrane curvature. (wikipedia.org)
Many IDPs undergo profound conformational conversion thatis often coupled to amyloid aggregation in the presence of negatively charged lipid membranes. (ias.ac.in)

Ultimately, specific lipid-protein contacts are revealed by intermolecular 1 H, 1 H nuclear Overhauser effect signals. (mappingignorance.org)

The protein phosphorylation-driven conformational change of p47(phox) enables its PX domain to bind to phosphoinositides, the interaction of which plays a crucial role in recruitment of p47(phox) from the cytoplasm to membranes and subsequent activation of the phagocyte oxidase. (embl.de)
The phox homology (PX) domain protein interaction network in yeast. (embl.de)
Here we show for the first time by genome-wide two-hybrid screens and in vitro binding assays that the PX domain is a bona fide protein interaction domain. (embl.de)
Diffusion of the complex E1/E2-EGFR-SCARB1-CD81 to the cell lateral membrane allows further interaction with Claudin 1 (CLDN1) and occludin (OCLN) to finally trigger HCV entry (PubMed:12970454, PubMed:24038151, PubMed:12913001, PubMed:20375010, PubMed:19182773) (By similarity). (proteopedia.org)
For instance, H/F substitution alters the charge distribution in the acyl chain, Also, interaction with membrane proteins may be affected As a drawback for NMR studies, H/F substitution reduces the 1 H density and, thus, coverage of (fluoro)lipid-protein contacts via 1 H, 1 H NOE signals. (mappingignorance.org)

The classic workaround to correlate 1 H with the better resolved 13 C frequencies is impractical for lipids where the required 13 C isotope enrichment is costly, may not resolve all overlap problems, and provides no distinct molecular marker from a likewise 13 C labelled protein to single out inter- from intramolecular NOE signals. (mappingignorance.org)
In summary, the fully resolved 1 H NMR spectrum of 4F-DHPC 7 micelles validates the minimal fluorination scheme for lipid acyl chains and the utility of fluorine as intramolecular shift reagent to enhance their 1 H signal dispersion. (mappingignorance.org)

Journal of Membrane Science. (lu.se)

From there, topics covered recent progress on which biophysical forces contribute to stabilizing the native membrane protein fold and how lipid membranes, detergents or surfactants influence the conformational space these important proteins sample. (biophysics.org)

Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. (embl.de)
Recent developments in the field of nanopore sensors allow the non-stochastic quantification of metabolites, where a nanopore is acting as an electrical transducer for selective substrate binding proteins (SBPs). (bvsalud.org)

Intrinsically disordered proteins (IDPs) belong to an important class of proteins that do not fold up spontaneously. (ias.ac.in)
Although High Speed Atomic Force Microscopy (HS-AFM) images label-free samples such as DNA, soluble proteins, MPs, and intrinsically disordered proteins at ~ 1n~m lateral, ~0.1 nm vertical and ~100 ms temporal solution in aqueous environment and at ambient temperature and pressure, its temporal resolution is too slow to characterize many dynamic biological processes. (nanoworld.com)
Such components could be e.g. intrinsically disordered proteins, flexibly interacting proteins and RNAs, lipids and membranes. (scale-frankfurt.org)

It is thus thought to facilitate membrane-vesicle fusion and fission in a manner analogous to clathrin-mediated endocytosis. (wikipedia.org)
To achieve this, the neuronal SNARE (i.e., soluble N -ethylmaleimide-sensitive factor attachment protein receptor) complex brings the vesicle and presynaptic membranes in close proximity, thereby, mediating the fusion of the two membranes resulting in exocytosis of neurotransmitters. (nature.com)
The vesicle membrane and the tail tube are clearly continuous. (asmblog.org)

The "Membrane Protein Folding" meeting, organized jointly by the Biophysical Society and the Korean Institute for Advanced Studies, held in Seoul mid-May, provided a vibrant stage for membrane protein folding scientists to exchange ideas and hold stimulating discussions. (biophysics.org)
This protocol can be applied to virtually any integral membrane protein amenable to classic biophysical studies and for which a near-atomic-resolution structure or homology model is available. (uiuc.edu)
In summary, the work presented here uncovers mechanistic details of physiologically crucial proteins, and serves as a stepping stone towards data-driven biophysical research and drug discovery. (kth.se)

Dominance analysis of competing protein assembly pathways. (mpg.de)
Affects various cell signaling pathways, host immunity and lipid metabolism (Probable). (proteopedia.org)

Titrations of this domain with lipid micelles, in the absence and presence of phorbols, indicate selective broadening of some resonances. (embl-heidelberg.de)
Commonly, micelles (spherical aggregates formed by short-chain lipids above a critical micelle concentration) or bicelles (discoidal aggregates formed by mixing short- and long-chained lipids) solubilise and stabilise membrane proteins for crystallisation and solution state Nuclear Magnetic Resonance (NMR) analysis. (mappingignorance.org)

2004 ). A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. (ibpc.fr)

Sticholysins I and II, cytolysins purified from the sea anemone Stichodactyla helianthus, act by lysing biological and model membranes. (bvsalud.org)

Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
Membrane fluctuations and acidosis regulate cooperative binding of "marker of self" CD47 with macrophage checkpoint receptor SIRPα. (mpg.de)

First the phage is met by the outer membrane (OM)-a barrier to surmount that also can be used as a convenient handgrip for adsorption. (asmblog.org)

Here, we show the effect of a well-known anionic lipid mimetic, sodium dodecyl sulfate (SDS), on the aggregation mechanism of a model amyloidogenic IDP, namely, bovine κ-casein. (ias.ac.in)

A multiple alignment of representative PX domain sequences from eukaryotic proteins [ ( PUBMED:9687503 ) ], shows relatively little sequence conservation, although their structure appears to be highly conserved. (embl.de)

However, the mechanism of gating on the cytoplasmic side of the membrane remains ambiguous. (elifesciences.org)

Biological membranes define the boundaries of cells and are composed primarily of phospholipids and membrane proteins. (uiuc.edu)

Perilipin 5 S155 phosphorylation by PKA is required for the control of hepatic lipid metabolism and glycemic control. (uci.edu)
We detected phosphorylation on S155 and identified S155 as a functionally important site for lipid metabolism. (uci.edu)
Alters lipid metabolism by interacting with hepatocellular proteins involved in lipid accumulation and storage (PubMed:14602201). (proteopedia.org)

A mesoscale stochanstic membrane model has been implemented to investigate the membrane deformations during the entry process. (upenn.edu)

NMR spectroscopy, contrarily, does not require molecular order and lipid-protein contacts may be sampled at low resolution to gauge protein insertion into the lipid layer, e.g. by use of paramagnetic agents that perturb the NMR signals of nearby membrane protein atoms. (mappingignorance.org)

DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
Classical protein kinase C (PKC) family members are activated by the binding of various ligands to one of several cysteine-rich domains of the enzyme. (embl-heidelberg.de)

His studies eventually led to the problems of transport of ions across cell membranes. (iupac.org)

Although focal adhesions are similar, in terms of molecular composition, to nascent adhesions and focal complexes, several adhesion proteins appear to change as focal adhesions evolve in response to tension, e.g., zyxin and vinculin . (cellmigration.org)

The methodology will include site-directed mutagenesis, covalent protein modifications, patch- clamp measurements, thermodynamic and kinetic analysis of dose-response curves and rates of transitions, videomicroscopy, and molecular modeling. (usda.gov)
During the previous funding period, we have been able to determine the overall structure of MscL, its topology, secondary structure and multimerism, and to evaluate energetic and spatial parameters that characterize conformational transitions in native MscL. (usda.gov)
A deep understanding of all the stages of conformational transitions preceding the fusion of viral and cell membranes is necessary for the development of specific inhibitors of viral reproduction. (bvsalud.org)

Phospholipase D is an important player in many physiological processes, including membrane trafficking, cytoskeletal reorganization, receptor-mediated endocytosis, exocytosis, and cell migration. (wikipedia.org)
Here we show in atomistic detail how the human β2-adrenergic receptor (β2AR) - a prototypical G protein-coupled receptor - is modulated by cholesterol in an allosteric fashion. (helsinki.fi)
Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. (uci.edu)
Ser/Thr protein kinases such as the Akt/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. (embl.de)

A minimal fluorination scheme for acyl chains is derived, and it is shown that a single hydrogen-to-fluorine substitution induces a fully resolved 1 H spectrum for 4F-DHPC 7 , a representative fluorolipid, without impairing micelle formation or stabilisation of the model membrane protein pSRII. (mappingignorance.org)

Fusion of synaptic vesicles with the presynaptic membrane occurs in the presence of the NSF (N-ethylmaleimide-sensitive-factor) / α-SNAP (α-soluble NSF attachment protein) disassembly machinery. (nature.com)
Stepwise remodeling and subcompartment formation in individual vesicles by three ESCRT-III proteins. (mpg.de)
Curvature-mediated assembly of Janus nanoparticles on membrane vesicles. (mpg.de)

Fusion with the host cell is most likely mediated by both E1 and E2, through conformational rearrangements of the heterodimer required for fusion rather than a classical class II fusion mechanism (PubMed:16533059, PubMed:29505618, PubMed:24698129). (proteopedia.org)

Others are components of the protein-rich internal membrane that lines the inside of the protein capsid. (asmblog.org)

Higher resolution analyses of lipid-protein contacts require more range-limited, directed, and unambiguous NMR indicators. (mappingignorance.org)

Wetting and complex remodeling of membranes by biomolecular condensates. (mpg.de)

Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)

Membrane morphologies induced by mixtures of arc-shaped particles with opposite curvature. (mpg.de)

It resides in the inner membrane of Escherichia coli and serves as protective safety valve in the event of osmotic shock. (usda.gov)
They then export some of these proteins as well some mitochondrially-encoded proteins to the inter membrane space, or they insert them into the inner membrane. (tcdb.org)
Mitochondria inherited three inner membrane translocases Sec, TAT and Oxa1 (YidC) from its bacterial ancestor. (tcdb.org)

We present a comprehensive list of 54 C1 domains occurring singly or doubly in 34 different proteins. (embl-heidelberg.de)
This core is composed of three different proteins, in quantities of ten, eight, and four respectively. (asmblog.org)
Its genome travels between hosts in a rather complicated virion containing 18 different proteins. (asmblog.org)
Its icosahedral capsid is correspondingly small (26 nm diameter) and simple (four different proteins). (asmblog.org)

2007 ). Several Oxa1-type insertases in the ER serve as catalytically active core subunits in the ER membrane protein complex (EMC), the guided entry of tail-anchored (GET) and the GET- and EMC-like (GEL) complex. (tcdb.org)
Mitochondrial TAT transports folded proteins in those eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. (tcdb.org)

Recently, molecular simulation techniques such as EBMetaD have been developed that create a conformational ensemble matching an experimental distance distribution while applying the minimal possible bias. (uni-regensburg.de)
Moreover, it has been proposed that the work required during an EBMetaD simulation to match an experimentally determined distribution could be used as a metric with which to assign conformational states to a given measurement. (uni-regensburg.de)

Taxonomy and function of C1 protein kinase C homology domains. (embl-heidelberg.de)
These domains were first discovered as the loci of phorbol ester and diacylglycerol binding to conventional protein kinase C isozymes, which contain 2 C1 domains (C1A and C1B) in their N-terminal regulatory regions. (embl-heidelberg.de)

The H/F substitution in the lipid provokes notable CSP for amide groups near both ends of the transmembrane seven-helix bundle indicating its asymmetric micelle insertion similar to paramagnetic markers. (mappingignorance.org)

There are over 800 different GPCRs in human cells, making them the largest family of cell surface proteins. (helsinki.fi)

MscL, a mechanosensitive channel of large conductance, was the first isolated molecule shown to respond to membrane stretch by opening a large aqueous pore. (usda.gov)
For such studies, membranes are often mimicked by different aqueous lipid dispersions that form subject to parameters like lipid type, acyl chain length, concentration, mixture and stoichiometry. (mappingignorance.org)

Anatomy33

Organisms8

Chemicals and Drugs84

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes81

Disciplines and Occupations5

Technology, Industry, Agriculture7

Information Science6

Health Care2

Bilayers8

Interactions28

Bilayer12

Plasma membrane6

Receptors7

Cell membrane9

Molecules8

Modulate3

Structural12

Biomolecules2

Dynamics9

Bound11

Simulations7

Hydrophobic2

Negatively charged2

Specific lipid-protein1

Interaction5

Intramolecular2

JOURNAL OF MEMBRANE SCIENCE1

Detergents1

Substrate2

Intrinsically3

Vesicle3

Biophysical3

Pathways2

Micelles2

Soluble proteins1

Model membranes1

Regulate2

Outer1

Anionic1

Eukaryotic proteins1

Cytoplasmic side1

Biological1

Metabolism3

Deformations1

Spectroscopy1

Ligands2

Transport of ions1

Adhesion1

Transitions3

Receptor4

Induces1

Vesicles3

Rearrangements1

Internal membrane1

Analyses1

Biomolecular1

Cytoskeletal1

Curvature1

Inner membrane3

Different proteins4

Subunits2

Simulation2

Kinase2

Micelle1

GPCRs1

Aqueous2