Derived from Haloarcula marismortui bacteriorhodopsin1
Here, we present a novel epitope tagging system comprised of a monoclonal antibody named GD-26, which recognises the TD peptide (GTGATPADD) derived from Haloarcula marismortui bacteriorhodopsin I (HmBRI) D94N mutant. (bvsalud.org)
Rhodopsin4
Bacteriorhodopsin belongs to the microbial rhodopsin family. (wikipedia.org)
however, the functions of rhodopsin and bacteriorhodopsin are different, and there is limited similarity in their amino acid sequences. (wikipedia.org)
Both rhodopsin and bacteriorhodopsin belong to the 7TM receptor family of proteins, but rhodopsin is a G protein-coupled receptor and bacteriorhodopsin is not. (wikipedia.org)
Our results show substrate binding to the dark-adapted RGC and GC alike and reveal differences between the constructs attributable to the regulatory influence of the rhodopsin on the conformation of the binding pocket. (elifesciences.org)
Proton9
Bacteriorhodopsin is a light-driven proton pump. (wikipedia.org)
H. salinarum expresses the membrane protein bacteriorhodopsin, which acts as a light-driven proton pump. (wikipedia.org)
Upon absorption of a photon, retinal changes its conformation, causing a conformational change in the bacterioopsin protein, as well, which drives proton transport. (wikipedia.org)
The retinal changes its ground state conformation upon binding of a proton, causing the Br to change conformation to the activated state and pump the proton. (proteopedia.org)
Xanthorhodopsin: a bacteriorhodopsin-like proton pump with a carotenoid antenna. (proteopedia.org)
The proton-conducting pathway of bacteriorhodopsin (BR) contains at University, PO Box 124 least nine internal water molecules that are thought to be key players in SE-22100 Lund, Sweden the proton translocation mechanism. (lu.se)
Bacteriorhodopsin (BR), the light-driven proton two half-channels. (lu.se)
Proton transfers plus the Schiff base connectivity switch In proton pumps, as initially shown for BR from Halobacterium salinarum, the dark conformation exhibits an outwardly-connected protonated Schiff base poised for proton release to an exterior half-channel. (pteninhibitor.com)
An example is the protein bacteriorhodopsin, a light driven proton pump in archaebacteria. (uiuc.edu)
Monoclonal antibody GD-261
In addition to already-existing epitope tags such as the FLAG tag and the ALFA tag adopting either extended or α-helix conformations, the unique 310 helix conformation of the TD peptide together with the corresponding monoclonal antibody GD-26 offers a novel tagging option for research. (bvsalud.org)
Retinal2
The nine stages are: bR + photon → K ⇌ L ⇌ M1 ⇌ M2 ⇌ M2' ⇌ N ⇌ N' ⇌ O ⇌ bR Bacteriorhodopsin in the ground state absorbs a photon and the retinal changes isomerization from all-trans 15-anti to the strained 13-cis 15-anti in the K state. (wikipedia.org)
The retinal adopts a less strained conformation to form the L intermediate. (wikipedia.org)
Protein8
Bacteriorhodopsin (Bop) is a protein used by Archaea, most notably by haloarchaea, a class of the Euryarchaeota. (wikipedia.org)
Bacteriorhodopsin is a 27 kDa integral membrane protein usually found in two-dimensional crystalline patches known as "purple membrane", which can occupy almost 50% of the surface area of the archaeal cell. (wikipedia.org)
Bacteriorhodopsin is synthesized as a protein precursor, known as bacterio-opsin, which is extensively modified after translation. (wikipedia.org)
Bacteriorhodopsin (Br) is a membrane protein in Archaea which moves protons across the cell membrane [1] . (proteopedia.org)
[4] Computational techniques such as molecular dynamics simulations can be used in conjunction with empirical structure determination strategies to extend and study protein structure, conformation and function. (cloudfront.net)
Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). (cipsm.de)
Thus, this study defines the location of key residues in TM-A with respect to the lipid bilayer, describes the conformation of TM-A in a biomembrane mimic, presents a peptide-bilayer model useful in the consideration of local protein folding in the microsomal desaturases, and presents a model of arginine and tyrosine control of transmembrane protein stability and insertion. (shengsci.com)
Archaea1
By expressing bacteriorhodopsin, the archaea cells are able to synthesise ATP in the absence of a carbon source. (wikipedia.org)
Amino acid1
begingroup$ The conformation is highly coupled to the amino acid side chains. (stackexchange.com)
Moves1
Generates capped alanine tetrapeptide in an extended conformation, then use Monte Carlo Minimization with random torsional moves to find the global minimum energy structure. (wustl.edu)
Structure1
The TD peptide was found to adopt a 310 helix conformation within the binding cleft, providing a characteristic peptide structure for recognition by GD-26 Fab. (bvsalud.org)
Image1
In 1990, Richard Henderson produced the first three-dimensional, high resolution image of bacteriorhodopsin using cryogenic electron microscopy (cryo-EM). (cloudfront.net)
Found1
Bacteriorhodopsin is a light-driven H+ ion transporter found in some haloarchaea, most notably Halobacterium salinarum (formerly known as syn. (wikipedia.org)
Absorbing green1
Cleavage of the signal peptide, the first 13 amino acids at the N-terminus, and the conversion of residue Gln14 to pyroglutamate Removal of residue Asp262 at the C-terminus Bacteriorhodopsin molecule is purple and is most efficient at absorbing green light (in the wavelength range 500-650 nm). (wikipedia.org)
Light-driven4
Bacteriorhodopsin is a light-driven H+ ion transporter found in some haloarchaea, most notably Halobacterium salinarum (formerly known as syn. (wikipedia.org)
Bacteriorhodopsin is a light-driven proton pump. (wikipedia.org)
Here we present an atomic model for structural changes involved in the vectorial, light-driven transport of protons by bacteriorhodopsin. (nih.gov)
The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 Å. (iucr.org)
Conformational2
We recently proposed a model of bradykinin bound to the rat bradykinin B2 receptor that is constructed on the basis of structural homology modeling to the criomicroscopic structure of the seven transmembrane domains of bacteriorhodopsin, extensive conformational searches and experimental mutagenesis results. (nih.gov)
The distribution of these conformations and the kinetic barriers between the conformational states define the conformational energy landscapes of proteins. (purdue.edu)
Molecule1
Cleavage of the signal peptide, the first 13 amino acids at the N-terminus, and the conversion of residue Gln14 to pyroglutamate Removal of residue Asp262 at the C-terminus Bacteriorhodopsin molecule is purple and is most efficient at absorbing green light (in the wavelength range 500-650 nm). (wikipedia.org)
Membranes1
beqq ,pported from both cattle rhodopsin The demonstration that Raman spec- extracted into digitonin micelles (10) and troscopy can provide useful information intact bovine photoreceptor membranes (6, about the conformation of biological mole- 11). (docksci.com)
Crucial1
When you are on a crucial conformation town, you will find removed to an Amazon relative hay where you can illustrate more about the series and be it. (ludwigsburger-grundbesitz.de)
Interaction1
Her interaction of bacteriorhodopsin closely is industrial dynamics including experiments at ICG Communications, InteleNet Data Centers, and FirstWorld Communications. (ludwigsburger-grundbesitz.de)
Helices1
Two types of secondary structures are dominating the local conformations of a polypeptide chain: alpha (α) helices and beta (β) sheets (cf. (tu-muenchen.de)
Structural1
Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin. (nih.gov)
Relative1
new and public flak of suspected demo video post: participation for relative microspectrophotometry within the caret bacteriorhodopsin. (reisemarkt-hochheim.de)
Small1
This native state ensemble includes small fluctuations around the native conformation, partially unfolded forms, and even globally unfolded forms. (purdue.edu)