Confocal fluorescence microscopes. Medical search. Frequent questions

Confocal microscopy was then introduced in 1960 which decreased the background and exposure time of the sample by directing light to a pinpoint and illuminating cones of light into the sample. (wikipedia.org)
Fluorescence microscopy, with its molecular specificity, is one of the major characterization methods used in the life sciences to understand complex biological systems. (nature.com)
Here we introdue a DNA-barcoding method, resolution enhancement by sequential imaging (RESI), that improves the resolution of fluorescence microscopy down to the Ångström scale using off-the-shelf fluorescence microscopy hardware and reagents. (nature.com)
e , As super-resolution revolutionized fluorescence microscopy, RESI results in another paradigm shift by reapplying the concept of localization to super-resolution data. (nature.com)
Confocal laser microscopy uses this technique. (photonics.com)
The microscopy lab is home to a Nikon fluorescence microscope with TIRF and photoactivation capabilities and an Olympus laser scanning confocal microscope. (ualberta.ca)
Superresolution microscopy enables fluorescence imaging of structures too small for traditional methods, such as deconvolution and confocal microscopy. (labmanager.com)
In fact, confocal microscopy and widefield deconvolution microscopy both work by removing image blur, but do so by opposite mechanisms. (fsu.edu)
New cryo-light microscopy techniques like LIGHTNING and TauSense fluorescence lifetime-based tools reveal structures for cryo-electron microscopy. (leica-microsystems.com)
This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. (leica-microsystems.com)
Coral workflows by Leica Microsystems help users correlate fluorescence and electron microscopy (CLEM) data. (leica-microsystems.com)
In Optica , The Optical Society's journal for high-impact research, researchers from Boston University, USA show that their new 'multi-z' confocal microscopy system can image the brains of living mice at video rate and with a field of view larger than a millimeter. (news-medical.net)
The new microscope is based on confocal microscopy, a technique commonly used for cell imaging. (news-medical.net)
Confocal microscopy produces images with high resolution and contrast by using a physical pinhole to block out-of-focus light and let in-focus light through. (news-medical.net)
Our method benefits from the contrast of confocal microscopy while being able to extend to volumetric imaging without sacrificing speed,' said Badon. (news-medical.net)
The researchers demonstrated the multi-z confocal microscopy system by using it to image whole C. elegans worms, which are too large (500 to 800 microns long) to easily image all at once with a traditional confocal microscope. (news-medical.net)
This course will provide an introduction to fluorescence microscopy and live cell imaging and will be composed of lectures and associated hands-on sessions. (unibas.ch)
The lecture series covers basics of light and fluorescence microscopy and gives an overview on image processing. (unibas.ch)
Fluorescence microscopy of tissues , cells or subcellular structures is accomplished by labeling an antibody with a fluorophore and allowing the antibody to find its target antigen within the sample. (newworldencyclopedia.org)
Fluorescence microscopy, confirmed the presence of radiolabeled SiNPs-TZ (1:8 Hc-TZ) within tumor even at later times. (dovepress.com)
The Core Facility "Confocal and Multiphoton Microscopy" is a member of the Center for Translational Imaging MoMAN . (uni-ulm.de)
The Core Facility "Confocal and Multiphoton Microscopy" of the Faculty of Medicine has existed in its current size since spring 2013. (uni-ulm.de)
The facility provides expertise in the field of confocal and multiphoton microscopy, data analysis, evaluation and interpretation as well as the conception and implementation of experiments. (uni-ulm.de)
The Core Facility "Confocal and Multiphoton Microscopy" offers services and guidance for all scientific questions concerning live cell microscopy. (uni-ulm.de)
This approach generalizes across different modalities, including serially-sectioned scanning electron microscopy (sSEM) of genetically labeled and contrast enhanced processes, spectral confocal reflectance (SCoRe) microscopy, and high-energy synchrotron X-ray microtomography ( μ CT) of large tissue volumes. (springer.com)
Brain imaging techniques from microscopy such as serially-sectioned scanning electron microscopy (sSEM), high-energy synchrotron X-ray microtomography ( μ CT), and spectral confocal reflectance (SCoRe) microscopy provide high-quality images with nanoscale or single-neuron resolution. (springer.com)
Spinning Disk Confocal Microscopy - Spinning disk microscopy has advanced significantly in the past decade and now represents one of the optimum solutions for both routine and high-performance live-cell imaging applications. (fsu.edu)
The rapid expansion in biomedical research using live-cell imaging techniques over the past several years has been fueled by a combination of events that include dramatic advances in spinning disk confocal microscopy instrumentation coupled with the introduction of novel ultra-sensitive detectors and continued improvements in the performance of genetically-encoded fluorescent proteins. (fsu.edu)
Widefield fluorescence and brightfield microscopy are also being used more frequently for resolving complex fluorophore and absorbing dye mixtures, a trend that should continue into the future. (fsu.edu)
In our laboratories, you can use both standard microscope techniques, like scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM), as well as infra-red based analysis techniques and micro-computed tomography (micro-CT). (lu.se)
Wide-field fluorescence microscopy is an extensively used microscopy technique that illuminates the entire objective field of view (FOV) with excitation light and collects the resulting fluorescence signal. (lu.se)
carry out studies where biological structures or functions are visualised using methods based on fluorescence microscopy. (lu.se)
understand the limitations and possibilities when using different methods based on light and fluorescence microscopy. (lu.se)
A theoretical introduction to microscopy with an emphasis on fluorescence microscopy. (lu.se)
Theoretical principles of confocal microscopy and deconvolution microscopy. (lu.se)
The course includes an introduction to microscopy, with emphasis on fluorescence microscopy, and theoretical principles of confocal microscopy and image deconvolution. (lu.se)
There is also an introduction to electron microscopy and some "imaging methods" that are not based on optical microscopes. (lu.se)
Cellomics Platform at MultiParkThe Cellomics ArrayScan VTI HCS Reader enables automated acquisition, visualization and quantification of a large number of images, with an automated fluorescence and brightfield microscopy allowing extraction of quantitative data from intensity and area measurement in high-throughput format. (lu.se)
Facilities for 3D optical imaging and electron microscopy at Materials Engineering with instruments including E-SEM FEI Quanta 200 MKII with analytical system Pegasus (EBSD+EDS) from EDAX and optical microscope Keyence VHX-6000. (lu.se)
Confocal fluorescence microscopy images of phospholipid bilayers supported by gallium phosphide nanowires. (lu.se)
Confocal laser scanning microscopy (CLSM) was used to evaluate qualitatively the enamel morphology. (bvsalud.org)
Confocal microscopy demon- exposures to workers likely during the production strated nanotubes within the nucleus that were in or use of commercial products. (cdc.gov)

Theoretical introduction to digital visualisation with an emphasis on fluorescence-based methods and digital image processing. (lu.se)
The course also includes a theoretical introduction to digital visualisation, with emphasis on fluorescence-based methods, including a brief introduction to ImageJ and basic analysis of digital images. (lu.se)

One of the pieces of art is an image of proteins in cancer cells taken with the ZEISS LSM 710 Laser Scanning Confocal Microscope , which defines new standards for sensitivity and flexibility in examining fluorescent biological specimens. (labmanager.com)
A Micro-Drill Tool Tip is another image turned into art and was taken using the ZEISS LSM 700 confocal microscope . (labmanager.com)
The final piece of art was created using the ZEISS LSM 710 Laser Scanning Confocal Microscope . (labmanager.com)

These standard microscopes are used for quick observation and imaging in brightfield (transmitted) or fluorescence modes. (ucl.ac.uk)

Practically any image acquired on a digital fluorescence microscope can be deconvolved, and several new applications are being developed that apply deconvolution techniques to transmitted light images collected under a variety of contrast enhancing strategies. (fsu.edu)

2/ 3 · use various types of advanced research microscopes, and imaging methods which are not based on optical microscopes. (lu.se)
We give an overview of different types of advanced research microscopes and the principles of the most common advanced methods. (lu.se)

Widefield Fluorescence was introduced in 1910 which was an optical technique that illuminates the entire sample as seen in Figure 1. (wikipedia.org)
In this mechanism, one can easily switch between standard widefield fluorescence and TIRF by changing the off-axis position of the beam focus at the objective's back focal plane. (wikipedia.org)
However, a majority of the deconvolution experiments reported in the literature apply to images recorded on a standard widefield fluorescence microscope. (fsu.edu)

In addition to serving as a standalone confocal or super-resolution microscope, the Nikon N-SIM/N-STORM/A1 system also allows researchers to perform correlative confocal-super-resolution imaging. (edu.hk)
The SIM-E is a fluorescence super-resolution microscope, the Ti2 is a fluorescence microscope for live imaging of pathogens, BSL2-compliant and the TE-300 is a fluorescence wide-field microscope. (lu.se)

Another disadvantage is that in most configurations based on the inverted microscope designs, the illumination is introduced on the specimen side opposite of the objective optics which requires imaging of the evanescent field region through the bulk of the specimen. (wikipedia.org)
Incorporating unique and powerful functions in a reliable, high performance system, ideally suited to the research and analytical lab, the XploRA PLUS is our best multi-sample, multi-user Raman imaging microscope ever. (horiba.com)
The SWIFT Fast Raman images are the fastest fully confocal Raman images available, typically 10x faster than conventional Raman imaging. (horiba.com)
The range of magnifications (2x-63x) and fluorescence filter blocks will satisfy most imaging applications. (ucl.ac.uk)
Researchers have developed a microscope specifically for imaging large groups of interacting cells in their natural environments. (news-medical.net)
The researchers also tailored the microscope for larger scale imaging than conventional confocal microscopes and designed it to image at video rate. (news-medical.net)
The participants will set-up experiments on state-of-the-art microscopes and analyze the resulting imaging data. (unibas.ch)
Laser scanning microscopes with highly sensitive detectors and ultra-short pulsed femtosecond laser technology are available, as well as inverted microscopes for confocal long-term imaging. (uni-ulm.de)
Instruments equipped for spectral imaging are becoming increasingly popular and many confocal microscopes now offer this capability. (fsu.edu)
Simply attach this MEMS confocal unit to an inverted microscope and enable confocal fluorescence imaging. (hamamatsu.com)
Fluorescence microscopes and live cell imaging for microbiology at Dept. of Biology. (lu.se)
Live Cell Imaging Platform at MultiParkNikon Ti-E is a fully motorised research microscope with Spectra X light engine and EMCCD, suitable for imaging of fluorescence labelling, such as DAPI, CFP, GFP, YFP, mCherry and Cy5. (lu.se)
Confocal Microscope Platform at MultiParkLeica SP8 confocal microscope for imaging of fixed tissue and cells. (lu.se)
The Zeiss 780 confocal laser-scanning inverted microscope allows for high-resolution imaging of fixed unstained and stained fluorescent samples and time-lapse imaging of live-cells. (lu.se)

Our approach, based on reversibly switchable fluorescent proteins, features three distinctly modulated illumination patterns crafted and combined to gain fluorescence ON-OFF switching cycles and image contrast. (nature.com)
A student in the Weissman Lab uses L&C's confocal microscope, a specialized instrument that generates high-resolution images of material stained with fluorescent probes. (lclark.edu)
The mobility of a fluorescent protein fusion can be assessed using a technique known as fluorescence recovery after photobleaching (FRAP). (microscopyu.com)
The decay times of this fluorescence is of the order of nanoseconds since the duration of the light depends on the lifetime of the excited states of the fluorescent material, in this case anthracene or stilbene. (newworldencyclopedia.org)
The common fluorescent tube relies on fluorescence. (newworldencyclopedia.org)
Biological molecules can be tagged with a fluorescent chemical group (fluorophore) by a simple chemical reaction , and the fluorescence of the tag enables sensitive and quantitative detection of the molecule. (newworldencyclopedia.org)
The overall goal of this procedure is to enable time-lapse analysis of diverse developmental processes using a fluorescent dissecting microscope with simple strategies for realignment after drifting in any direction. (jove.com)
A confocal scanner then reads the fluorescent intensity of each hybridized sequence in the array. (medscape.com)
Prostate specimens from subjects undergoing radical prostatectomy (RP) will be stained with a fluorescent dye (Histolog Dip) and then be scanned on a digital fluorescent confocal microscope (FCM) known as the Histolog Scanner. (who.int)

The LSM 710 confocal microscope has increased sensitivity, a higher signal-to-noise ratio, improved flexibility for new fluorescence dyes and multimodal experiments, as well as new multiphoton detectors, which allow for deeper optical penetration into biological structures. (labmanager.com)
Instruction in the confocal and multiphoton microscope with the aim of working independently. (uni-ulm.de)

This inverted confocal microscope includes a motorized stage and a two channel confocal scanning head with spectral detection. (lu.se)
The instrument employs the spectral detector GaAsp that allows for high sensitivity detection of 32-channels and three additional detectors (PMTs), two for confocal detection and one for transmitted light detection. (lu.se)

Researchers from the Institute for Genomic Biology (IGB) Core Facilities, affiliated with the University of Illinois at Urbana-Champaign, used state-of-the-art microscopes for pioneering research to capture images that address significant problems facing humanity related to health, agriculture, energy and the environment. (labmanager.com)

The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift. (jove.com)

Here, the coordinate targeted fluorescence ON-OFF switching of the rsFP requires intensities in the range of W-kW cm −2 to produce images with sub-100 nm spatial resolution. (nature.com)
The Immunochromato Reader (Lateral flow reader) quickly and quantitatively measures the color intensities of red, blue and fluorescence based immunochromatographic lateral flow reagents. (hamamatsu.com)
After staining, the fluorescence intensities of sections of Cd2+-positive roots were compared with those of Cd2+-negative roots under a laser confocal scanning microscope, and the location of colloidal gold particles was determined with a transmission electron microscope. (cdc.gov)

HORIBA the world leader in fluorescence spectroscopy research instrumentation including steady state fluorometers fluorescence spectrometers and modular spectrofluorometer solutions. (biophysics.org)

This is not strictly true because images acquired using a pinhole aperture in a confocal microscope can also be analyzed by deconvolution techniques. (fsu.edu)
Rather than having one pinhole, like traditional confocal setups, the new microscope has a series of reflective pinholes that each capture in-focus light from a different depth within the sample. (news-medical.net)

Our light microscopes include widefield and laser scanning confocal systems. (ucl.ac.uk)
The laser light is intended to scan the sampled plane and acquire information from fluorescence of the observed ample. (photonics.com)
This confocal laser scanning microscope has a compact design and performs fluorescence measurements, creates optical sections of samples, and combines them into 3D image stacks. (labmanager.com)
Motorized confocal laser scanning microscope equipped with both a high-resolution galvano scanner and a high-speed (15 fps) 1024 x 1024 resonant scanner capable of capturing high-quality confocal images of tissues, cells and molecular events at high speed and enhanced sensitivity. (lu.se)
Motorized confocal laser scanning microscope equipped with a high-resolution galvano scanner capable of capturing high-quality confocal images of tissues, cells and molecular events at enhanced sensitivity. (lu.se)

Fluorescence is a luminescence that is mostly found as an optical phenomenon in cold bodies, in which the molecular absorption of a photon at a certain wavelength triggers the emission of another photon with a longer wavelength . (newworldencyclopedia.org)
High speed, kinetic plate imager for fluorescence / luminescence assays in GPCR / ion channel drug discovery field. (hamamatsu.com)

Associated equipment includes a controlled atmosphere incubator, an inverted light microscope and a benchtop centrifuge. (ualberta.ca)

Nikon SIM-E Microscope, Nikon Ti2 Pathogen Live Microscope and Nikon TE-300 Microscope at IKVL, Nordenfelt Lab (Quantitative infection biology). (lu.se)

Technique using functional optical microscope with the addition of a coherent source collinear with the image path. (photonics.com)
Routinely applied to widefield optical sections, as well as those obtained in confocal and structured illumination, the technique has benefited from the continued development of advanced algorithms and turnkey systems. (fsu.edu)

Two-dimensional images of the fluorescence can then be obtained, although there are also mechanisms in which three-dimensional information on the location of vesicles or structures in cells can be obtained. (wikipedia.org)
Using the different microscopes instruments and being co-localized allows a comprehensive characterization of the sample and a precise superimposition of all the images. (horiba.com)
The final confocal images can then be overlaid with the corresponding super-resolution images in the same FOV. (edu.hk)
A 3D fast Fourier transformation of a cell image was taken with the ZEISS ELYRA S.1 superresolution structured illumination system , which images fluorophores with up to twice the resolution of a conventional light microscope. (labmanager.com)
Deconvolution is a computationally intensive image processing technique that is being increasingly utilized for improving the contrast and resolution of digital images captured in the microscope. (fsu.edu)
The foundations are based upon a suite of methods that are designed to remove or reverse the blurring present in microscope images induced by the limited aperture of the objective. (fsu.edu)
Modern deconvolution algorithms have yielded images of comparable resolution to that of a confocal microscope. (fsu.edu)
Plate Runner Platform at MultiParkHigh content screening (HCS) / Analysis (HCA) enables automated acquisition, visualization and quantification of a large number of images such as rapid snapshot acquisition of fluorescence images of cells and small organisms. (lu.se)
The Olympus VS-120 Virtual Slide Microscope allows for fully automated high-speed and high-resolution bright field and multi-fluorescence digital images of entire histological slides or selected areas within them. (lu.se)

Motorised wide-field fluorescence microscope with large field of view equipped with a monochrome sCMOS camera as well as a color camera. (lu.se)

Highly configurable, this microscope has a variety of excitation and detector options and can be upgraded with high speed, confocal and superresolution. (labmanager.com)
Fluorescence in several wavelengths can be detected by an array detector, to detect compounds from HPLC flow. (newworldencyclopedia.org)

However, the current approaches to fluorescence nanoscopy, even if powerful, are often limited by high doses of light, low contrast, small fields of view or slow recording times. (nature.com)
This nanoscope features light patterns with optimized shape and periodicities to switch ON, OFF and read out the fluorescence of the rsFPs. (nature.com)
Although extended illumination and reflective pinholes have been used before, this is the first time they were combined in a confocal microscope setup in a light-efficient way. (news-medical.net)
LBIC gives researchers access to 8 advanced light microscopes, all placed at Biomedicinskt Centrum (BMC). (lu.se)

A suite of 15 cell culture clean-rooms each equipped with Laminar Flow Cabinets, carbon dioxide incubators, inverted microscopes, etc. (dcu.ie)

The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. (nature.com)

Production and optimisation of both fixed and living microscope preparations. (lu.se)

In cell and molecular biology, a large number of molecular events in cellular surfaces such as cell adhesion, binding of cells by hormones, secretion of neurotransmitters, and membrane dynamics have been studied with conventional fluorescence microscopes. (wikipedia.org)

STED microscope from Abberior Instruments combined with FLIM and polarization. (lu.se)

The Zeiss upright microscopes are optimised for fixed specimens and the inverted Leica microscopes can take a variety of sample vessels. (ucl.ac.uk)

It is fully confocal, not compromising image quality, spatial or depth resolution. (horiba.com)

This guide is intended to provide personnel contacts and information relating to our SERI core facility microscopes. (masseyeandear.org)

Alternatively, researchers can choose to use confocal to scan a larger FOV to provide an overview of the sample, and use super-resolution to image a selected area of interest to examine the area in greater detail. (edu.hk)
This specialized microscope allows them to image the process in a high-resolution way, as well as to make observations over time. (lclark.edu)
An image of the Birefringence of Glucose Monohydrate was taken with the ZEISS Axiovert.A1 200M This model has been replaced with the ZEISS Axio Observer , an inverted microscope with extensive performance capabilities. (labmanager.com)
Fast image acquisition was important because the fluorescence indicators that monitor cellular function typically operate on time scales of a few tens of milliseconds. (news-medical.net)
They then used their microscope to image the hippocampal region of a mouse brain in an awake animal whose head was kept stationary. (news-medical.net)

The full objective lens is then used to detect fluorescence, which provides high resolution. (news-medical.net)

For example, fluorescence is useful for lighting and tagging molecules in analytical chemistry and biochemistry. (newworldencyclopedia.org)

Instruction in the HTS confocal microscope for long-term live cell observations. (uni-ulm.de)

Analysis of simulated and experimental fluorescence recovery after photobleaching. (microscopyu.com)

Anatomy12

Organisms2

Chemicals and Drugs19

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes19

Disciplines and Occupations2

Technology, Industry, Agriculture3

Information Science4

Health Care1

Geographicals1

Microscopy42

Emphasis on fluorescence-based methods2

Zeiss LSM3

Brightfield1

Digital fluorescence1

Types of advanced research microscopes2

Widefield fluorescence3

Super-resolution microscope2

Imaging14

Fluorescent9

Multiphoton2

Spectral2

State-of-the-art micr1

Time-Lapse1

Intensities3

Spectrometers1

Pinhole2

Laser5

Luminescence2

Benchtop1

Quantitative1

Optical2

Images9

Monochrome1

Detector2

Light4

Incubators1

Contrast1

Optimisation1

Molecular1

FLIM1

Upright1

Spatial1

Core facility1

Image5

Objective1

Analytical1

Live1

Experimental1