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FluorescenceMicroscopy, ConfocalSpectrometry, FluorescenceMicroscopy, FluorescenceFluorescent DyesFluorescence Resonance Energy TransferFluorescence PolarizationIn Situ Hybridization, FluorescenceFluorescence Recovery After PhotobleachingGreen Fluorescent ProteinsRhodaminesLuminescent ProteinsFluorescein-5-isothiocyanateCarbocyaninesFluoresceinsDiffusionKineticsUnilamellar LiposomesProtein BindingCell MembraneDextransRecombinant Fusion ProteinsMicroscopy, Fluorescence, MultiphotonLacrimal ApparatusCell LineCalciumEndocytosisTime FactorsMolecular Sequence DataLipid BilayersCytoplasmMembrane MicrodomainsPhosphatidylcholinesTryptophanCells, CulturedEnergy TransferCell NucleusFluorescent Antibody TechniquePolymersBiological TransportFlow CytometryAmino Acid SequenceDNATransfectionMicroscopy, Electron, TransmissionActinsRabbitsCell Line, TumorFluorometryProtein TransportOptical ImagingMembrane ProteinsCytoskeletonSubcellular FractionsModels, BiologicalPhotobleachingHeLa CellsPhotonsProtein ConformationFluorescence Polarization ImmunoassayCircular DichroismAnilino NaphthalenesulfonatesFluoresceinImmunohistochemistry2-NaphthylamineChlorophyllHydrogen-Ion ConcentrationLasersCholesterolAcrylamideEpithelial CellsBinding SitesTemperatureSignal TransductionBlotting, WesternPyrenesDansyl CompoundsRats, Sprague-DawleyDiphenylhexatrieneLightNaphthalenesulfonatesPhotochemistrySpectrometry, X-Ray EmissionBiophysicsMolecular ImagingStaining and LabelingLiposomesQuantum DotsSpectrophotometry, UltravioletModels, MolecularBiophysical PhenomenaProtein DenaturationAminolevulinic AcidIndocyanine GreenThermodynamicsMathematicsBiosensing TechniquesModels, ChemicalGuanidineMembrane Fluidity
MicroscopePrinciples of confocal microscopyWidefieldEmphasis on fluorescence-based methodsImagingExcitationMicroscopesDisk ConfocalBrightfield microscopyElectron MicroscopyMultiphoton microscopyHistologyResonance energy tSingle moleculeIntroduction to microscopyLaserCellsDetectorSpatialDynamicsLightImagesImageOpticalSuper-resolutionTissueTechniquesFacilityMethodsFieldScannerModeSignal
Microscope31
  • There is an inverted widefield fluorescent microscope in the Kroto Imaging area which is particularly good for imaging thin samples, even with low levels of fluorescence. (sheffield.ac.uk)
  • In addition, epifluorescent microscopes generally use the line-spectrum produced by a high pressure mercury bulb for sample excitation, whereas a confocal microscope uses monochromatic lasers to excite the sample. (sheffield.ac.uk)
  • The 2fFCS set-up is based on a specificly modified MicroTime 200 confocal microscope. (picoquant.com)
  • The MicroTime 200 time-resolved fluorescence microscope system is a powerful instrument capable of Fluorescence Lifetime Imaging (FLIM) with single molecule detection sensitivity. (picoquant.com)
  • In addition to serving as a standalone confocal or super-resolution microscope, the Nikon N-SIM/N-STORM/A1 system also allows researchers to perform correlative confocal-super-resolution imaging. (edu.hk)
  • Laser scanning cytometry (LSC) is a new technique that potentially fulfils these requirements by combining the benefits of FC with those of IA and is built around the epi-fluorescence microscope 10 . (ersjournals.com)
  • Confocal microscope (Leica TCS-SP8) equipped with 4 laser lines for fluorescence excitation (405 nm, 488 nm, 561 nm, and 638 nm), 4 detectors for simultaneous data acquisition, CO 2 and temperature control, a resonant scanner for live-cell studies, high content screening automation (HCS A) module, and super-resolution module (HyVolution II). (cipf.es)
  • Confocal microscope (Leica TCS-SP2-AOBS) equipped with 9 laser lines (allowing fluorescent excitation from 405 nm to 633 nm), 4 detectors for simultaneous data acquisition, a CO 2 and temperature control system for live-cell studies, and analysis software for fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), and fluorescence lifetime imaging microscopy (FLIM). (cipf.es)
  • Inverted fluorescence microscope (Zeiss Apotome II), equipped with a flexible LED light source (Colibri 7) allowing excitation from 405 nm to 633 nm. (cipf.es)
  • Inverted fluorescence microscope (Leica DM6000B) equipped with a total internal reflection fluorescence (TIRF) system and a CO 2 and temperature control system for live-cell studies. (cipf.es)
  • Upright fluorescence microscope (Leica DM6000B). (cipf.es)
  • Inverted fluorescence microscope (Zeiss Axiovert200M). (cipf.es)
  • Instruction in the confocal and multiphoton microscope with the aim of working independently. (uni-ulm.de)
  • Instruction in the HTS confocal microscope for long-term live cell observations. (uni-ulm.de)
  • Je travaille actuellement sur la d convolution aveugle param trique d'images de microscope balayage laser Confocal (CLSM). (inria.fr)
  • Le CLSM est un microscope optique fluorescence qui balaye des sections d'un sp cimen en 3D et emploie un trou d' pingle pour rejeter la plus grande partie de la lumi re en dehors du foyer. (inria.fr)
  • Cependant, la qualit des images du microscope confocal souffre de deux limitations physiques de base. (inria.fr)
  • D'abord, les taches floues en dehors du foyer dues la nature limit e par la diffraction du microscope optique et deuxi mement, le trou d' pingle confocal r duit drastiquement la quantit de photons d tect s par le photomultiplicateur, causant alors un bruit poissonnien. (inria.fr)
  • Nous surmontons ce probl me en employant un mod le physique d'acquisition du microscope et en pr sentant la connaissance a priori au sujet du sp cimen. (inria.fr)
  • We propose an alternate minimization algorithm for estimating the point-spread function (PSF) of a confocal laser scanning microscope and the specimen fluorescence distribution. (inria.fr)
  • This Cerna Microscope has been configured for epi-fluorescence and differential interference contrast (DIC) imaging. (thorlabs.com)
  • This Cerna microscope is configured for both epi-fluorescence and multiple trans-illumination imaging modalities. (thorlabs.com)
  • The most widely used three-dimensional imaging technology is the scanning confocal laser microscope. (photonics.com)
  • In our laboratories, you can use both standard microscope techniques, like scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM), as well as infra-red based analysis techniques and micro-computed tomography (micro-CT). (lu.se)
  • Prostate specimens from subjects undergoing radical prostatectomy (RP) will be stained with a fluorescent dye (Histolog Dip) and then be scanned on a digital fluorescent confocal microscope (FCM) known as the Histolog Scanner. (who.int)
  • Live Cell Imaging Platform at MultiParkNikon Ti-E is a fully motorised research microscope with Spectra X light engine and EMCCD, suitable for imaging of fluorescence labelling, such as DAPI, CFP, GFP, YFP, mCherry and Cy5. (lu.se)
  • Confocal Microscope Platform at MultiParkLeica SP8 confocal microscope for imaging of fixed tissue and cells. (lu.se)
  • This inverted confocal microscope includes a motorized stage and a two channel confocal scanning head with spectral detection. (lu.se)
  • The SIM-E is a fluorescence super-resolution microscope, the Ti2 is a fluorescence microscope for live imaging of pathogens, BSL2-compliant and the TE-300 is a fluorescence wide-field microscope. (lu.se)
  • The Olympus VS-120 Virtual Slide Microscope allows for fully automated high-speed and high-resolution bright field and multi-fluorescence digital images of entire histological slides or selected areas within them. (lu.se)
  • The Zeiss 780 confocal laser-scanning inverted microscope allows for high-resolution imaging of fixed unstained and stained fluorescent samples and time-lapse imaging of live-cells. (lu.se)
Principles of confocal microscopy2
Widefield2
  • Our light microscopes include widefield and laser scanning confocal systems. (ucl.ac.uk)
  • We provide several high-performance products, including epi-illuminator modules for one to six filter sets, Dodt gradient contrast and differential interference contrast (DIC) modules for transmitted light imaging, as well as scientific cameras and accessories for widefield and fluorescence imaging. (thorlabs.com)
Emphasis on fluorescence-based methods2
  • Theoretical introduction to digital visualisation with an emphasis on fluorescence-based methods and digital image processing. (lu.se)
  • The course also includes a theoretical introduction to digital visualisation, with emphasis on fluorescence-based methods, including a brief introduction to ImageJ and basic analysis of digital images. (lu.se)
Imaging16
  • The confocal imaging facility is located in the Kroto building, North Campus, and is part of the Department of Materials Science and Engineering. (sheffield.ac.uk)
  • The SWIFT Fast Raman images are the fastest fully confocal Raman images available, typically 10x faster than conventional Raman imaging. (horiba.com)
  • In vivo vMSOT brain imaging of P301L mice showed higher retention of PBB5 in the tau-laden cortex and hippocampus compared to wild-type mice, as confirmed by ex vivo vMSOT, epi-fluorescence, multiphoton microscopy, and immunofluorescence staining. (springer.com)
  • Digital pathology research scanner (Leica Aperio Versa) for brightfield, fluorescence, and fluorescence in situ hybridization (FISH) imaging, equipped with a 200-slide autoloader to allow unsupervised batch processing-from low-magnification to high-resolution or oil-immersion scanning. (cipf.es)
  • These standard microscopes are used for quick observation and imaging in brightfield (transmitted) or fluorescence modes. (ucl.ac.uk)
  • The range of magnifications (2x-63x) and fluorescence filter blocks will satisfy most imaging applications. (ucl.ac.uk)
  • The Core Facility "Confocal and Multiphoton Microscopy" is a member of the Center for Translational Imaging MoMAN . (uni-ulm.de)
  • Laser scanning microscopes with highly sensitive detectors and ultra-short pulsed femtosecond laser technology are available, as well as inverted microscopes for confocal long-term imaging. (uni-ulm.de)
  • 1 - Blind deconvoltion for thin layered confocal imaging . (inria.fr)
  • Photostable nanoparticles arrayed on glass slides for calibration of fluorescence imaging systems and quantitative analysis. (gracebio.com)
  • CoverWell ™ imaging chambers are designed to stabilize and support thick and free-floating specimens for confocal microscopy and imaging applications. (gracebio.com)
  • Lifetime Imaging and Fluorescence Correlation Spectroscopy. (iss.com)
  • Applications also include cell sorting, multiphoton imaging, matrix-assisted laser desorption ionization time-of-flight (Maldi-Tof) for protein analysis, fluorescence analysis in proteomics and genomics, and a novel treatment for ischemic strokes. (photonics.com)
  • Because the amount of laser energy that is actually absorbed is relatively low, multiphoton imaging is much more compatible with live-cell studies than is scanning confocal laser microscopy. (photonics.com)
  • One of the newest techniques combines fluorescence resonance energy transfer, or FRET, with either confocal or multiphoton imaging. (photonics.com)
  • Fluorescence microscopes and live cell imaging for microbiology at Dept. of Biology. (lu.se)
Excitation3
  • A new technique for the measurement of fluorescence lifetimes relies on the (near steady state) excitation with short optical pulses. (arizona.edu)
  • This allows the user to choose very specific fluorescence excitation wavelengths and expands the range of fluorophores that can be used together on a single sample. (sheffield.ac.uk)
  • The problem is that the confocal volume sensibly depends on numerous parameters of the optical set-up, refractive index mismatch of the sample solution and the objective's immersion medium, coverslide thickness variations and especially the dependence on optical saturation of the fluorescent dye, which can occur at even very low excitation powers. (picoquant.com)
Microscopes1
  • The facility contains two Zeiss LSM510 Meta confocal microscopes which can be booked and operated independently of each other. (sheffield.ac.uk)
Disk Confocal1
  • In parallel, using spinning disk confocal fluorescent microscopy (FM), single-molecule measurements, we investigate the average ensemble kinetics of the number of viruses which ejected their DNA versus time during an infection. (lu.se)
Brightfield microscopy1
  • Cellomics Platform at MultiParkThe Cellomics ArrayScan VTI HCS Reader enables automated acquisition, visualization and quantification of a large number of images, with an automated fluorescence and brightfield microscopy allowing extraction of quantitative data from intensity and area measurement in high-throughput format. (lu.se)
Electron Microscopy1
Multiphoton microscopy3
  • The Core Facility "Confocal and Multiphoton Microscopy" of the Faculty of Medicine has existed in its current size since spring 2013. (uni-ulm.de)
  • The facility provides expertise in the field of confocal and multiphoton microscopy, data analysis, evaluation and interpretation as well as the conception and implementation of experiments. (uni-ulm.de)
  • The Core Facility "Confocal and Multiphoton Microscopy" offers services and guidance for all scientific questions concerning live cell microscopy. (uni-ulm.de)
Histology1
Resonance energy t2
Single molecule3
  • The Fandango 515 nm solid-state laser from Cobolt AB offers up to 150 mW CW output power, making it ideal for fluorescence applications such as confocal microscopy and single molecule analysis. (laserfocusworld.com)
  • The Cobolt Fandango™, now available with up to 150 mW CW output power from the same small package, is not only perfectly suited for power demanding fluorescence analysis applications such as confocal microscopy, high-speed plate reading and single molecule analysis, but now with even higher output power, as a general all solid state alternative to bulky Ar+ gas lasers. (laserfocusworld.com)
  • Single-molecule fluorescence measurements of the ensemble kinetics for DNA ejection from WT phage λ over time in 10 mM MgCl2 Tris-buffer at 22 and 37°C. YOYO dye shows particles with ejected DNA stretched in the flow. (lu.se)
Introduction to microscopy1
Laser10
  • The novel technique has the potentiality to provide high time resolution - since it relies on the laser pulse duration, rather than on electronic gating techniques - and permits, in combination with bilateral confocal microscopy and the use of a (cooled) CCD, sensitive signal detection over a large dynamic range. (arizona.edu)
  • Laser scanning confocal microscopy is a significant advance in the field of optical microscopy, primarily because it permits sample visualization deeper within living and fixed cells, tissues and other samples. (sheffield.ac.uk)
  • The TCSPC time of each recorded photon is used to determine in which laser focus/detection region the fluorescence light was generated. (picoquant.com)
  • In addition to the autocorrelation curves, one can also correlate the fluorescence signal from one laser focus with that of the second focus. (picoquant.com)
  • 1 - Blind Deconvolution for Confocal Laser Scanning Microscopy . (inria.fr)
  • La microscopie confocale balayage laser, est une technique puissante pour tudier les sp cimens biologiques en trois dimensions (3D) par sectionnement optique. (inria.fr)
  • Confocal laser scanning microscopy is a powerful technique for studying biological specimens in three dimensions (3D) by optical sectioning. (inria.fr)
  • Probe-based confocal laser endomicroscopy (pCLE) provides real-time vision of respiratory tissues at the cellular level through a flexible bronchoscope. (archbronconeumol.org)
  • A two-dimensional (X-Y) image is built up by rastering the focused laser spot in the X-Y plane, detecting the position-dependent sample fluorescence. (photonics.com)
  • The laser beam is tightly focused within the sample so that it induces two-photon or even three-photon absorption at the beam waist - resulting in fluorescence that is collected in a way similar to that used in scanning confocal laser microscopy. (photonics.com)
Cells4
  • PCC 6803 cells using hyperspectral confocal fluorescence microscopy and multivariate image analysis. (osti.gov)
  • Photobleaching and low penetration depth, caused by the high scattering of the visible light, limit the utility of confocal microscopy in certain applications, particularly with living cells and/or thicker tissue samples. (photonics.com)
  • The qualitative analyses of neutral lipid bodies under stress conditions were performed by confocal microscopy and revealed bright golden-yellow lipid droplets in stress exposed cells. (hindawi.com)
  • Plate Runner Platform at MultiParkHigh content screening (HCS) / Analysis (HCA) enables automated acquisition, visualization and quantification of a large number of images such as rapid snapshot acquisition of fluorescence images of cells and small organisms. (lu.se)
Detector2
  • Fluorescence induced in the beam waist is imaged in a confocal arrangement onto an aperture before reaching a low-noise detector. (photonics.com)
  • The instrument employs the spectral detector GaAsp that allows for high sensitivity detection of 32-channels and three additional detectors (PMTs), two for confocal detection and one for transmitted light detection. (lu.se)
Spatial2
  • Combined with confocal microscopy it enables the spatial determination of the fluorescence lifetimes, the value of which is influenced by the local environment of fluorescent probe molecules in biological samples. (arizona.edu)
  • It is fully confocal, not compromising image quality, spatial or depth resolution. (horiba.com)
Dynamics2
  • It contains the complete optics and electronics for recording virtually all aspects of the fluorescence dynamics of microscopic samples or femtoliter volumes. (picoquant.com)
  • Articles, abstracts, and book chapters citing Globals Software (Globals Unlimited, Globals for Spectroscopy, and Globals for Images aka SimFCS) by authors not affiliated with the Laboratory for Fluorescence Dynamics (LFD). (uci.edu)
Light1
Images2
  • The final confocal images can then be overlaid with the corresponding super-resolution images in the same FOV. (edu.hk)
  • Confocal fluorescence microscopy images of phospholipid bilayers supported by gallium phosphide nanowires. (lu.se)
Image2
  • Alternatively, researchers can choose to use confocal to scan a larger FOV to provide an overview of the sample, and use super-resolution to image a selected area of interest to examine the area in greater detail. (edu.hk)
  • To date, where AM numbers are limited or if AM morphology is required, image analysis (IA) systems such as confocal microscopy have been used 8 . (ersjournals.com)
Optical1
  • The goal of the CIPF Optical and Confocal Microscopy Service is to provide infrastructure and technical support to CIPF researchers and users from other research institutions and companies. (cipf.es)
Super-resolution2
  • In the same field-of-view (FOV), researchers can choose to use confocal for certain fluorescence channels, and super-resolution for the remaining ones. (edu.hk)
  • Confocal and super-resolution microscopy. (cipf.es)
Tissue1
  • PBB5 showed specific binding to recombinant K18 tau fibrils by fluorescence assay, to post-mortem Alzheimer's disease brain tissue homogenate by competitive binding against [ 11 C]PBB3 and to tau deposits (AT-8 positive) in post-mortem corticobasal degeneration and progressive supranuclear palsy brains. (springer.com)
Techniques2
  • Fluorescence techniques are used and applied increasingly in academics and industry. (iss.com)
  • This annual workshop outlines fundamental concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. (iss.com)
Facility1
  • The facility also supports users of the Zeiss Lightsheet z.1, and Cellvizio Dual Band Invivo confocal. (sheffield.ac.uk)
Methods1
Field1
  • In this paper, we model the point-spread function (PSF) of a fluorescence MACROscope with a field aberration. (inria.fr)
Scanner1
  • A confocal scanner then reads the fluorescent intensity of each hybridized sequence in the array. (medscape.com)
Mode1
  • 20x water immersion objective confocal mode. (lu.se)
Signal1
  • Dose-dependent optoacoustic and fluorescence signal intensities were observed in the mouse brains following i.v. administration of different concentrations of PBB5. (springer.com)