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CentrifugationCentrifugation, Density GradientCentrifugation, ZonalCentrifugation, IsopycnicCell FractionationMetrizamideMicroscopy, ElectronMolecular WeightSubcellular FractionsPovidoneElectrophoresis, Polyacrylamide GelUltracentrifugationHypergravityCell SeparationFiltrationCell MembraneChromatography, GelKineticsTritiumSucroseOrganoidsCesiumMethodsCattleLiverSolubilityDetergentsChromatographySilicon DioxideNucleotidasesPC12 CellsDiatrizoateAcid PhosphataseRabbitsMacromolecular SubstancesLysosomesMicrosomesSpermatozoaMembranesCell LineChemical PrecipitationTemperatureChromatography, DEAE-CelluloseAmino AcidsTriiodobenzoic AcidsCytoplasmic GranulesColloidsCells, CulturedElectrophoresisCarbon IsotopesCell-Free SystemRats, Inbred StrainsOctoxynolMagnesiumPolyethylene GlycolsFreezingEscherichia coliDNAPhospholipidsChromatography, Ion ExchangeMicrobodiesPhosphotungstic AcidErythrocytesMembrane ProteinsProteinsChromatography, AffinityCytoplasmOrganellesBlood Specimen CollectionIntracellular MembranesCell NucleusSuccinate DehydrogenaseRibonucleasesPhosphorus IsotopesCarbohydratesAdenosine TriphosphatasesElectrophoresis, DiscPronaseVirus CultivationImmunodiffusionAgrocybeTime FactorsEdetic AcidSperm MotilityGlucose-6-PhosphataseRNAChemistry Techniques, AnalyticalMolecular Sequence DataChemical FractionationCulture MediaThymidineDialysisProtein BindingBacteriological TechniquesRibosomesViruses, UnclassifiedNucleoproteinsCulture TechniquesCytosolSurface-Active Agents

Density gradient centrifugationResuspendFiltrationSupernatantSucrosePelletMarrowAqueousEmulsionPhysiological conditionsTubesExtractionTimeCellsSelectivelyProcedureHighMethodEquivalentMediumProcessesPlasmaBloodVolumeVisitStandardFactorPotentialEquipmentPresentEffectsPatientsFreshStorage ConditionsEnvironmentalModernPlant

Density gradient centrifugation1

• This protocol has been optimized using fresh human PBMCs isolated using density gradient centrifugation. (biolegend.com)

Resuspend2

• Resuspend the pellet in PBS EDTA after the final centrifugation to obtain a total volume of approximately 300 µl of solubilized sample. (cdc.gov)
• Sediment the islets by centrifugation and resuspend the pellet in one milliliter of PBS supplemented with 50 micromolar PR619. (jove.com)

Filtration4

• Microvesicles and exosomes were obtained from conditioned medium by filtration and differential centrifugation. (karger.com)
• no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. (qiagen.com)
• The QIAfilter Plasmid Kits combines QIAfilter Cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tips containing anion-exchange resin for efficient plasmid purification. (qiagen.com)
• As an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation, you can use the QIAfilter Mega-Giga Cartridges (cat. (qiagen.com)

Supernatant1

• After centrifugation, the supernatant will be separated. (who.int)

Sucrose1

• Generally accepted solutions with meet these conditions are 0.9% saline, 5% dextrose, and 10% sucrose. (sigmaaldrich.com)

Pellet1

• Suspend the pellet obtained in the previous centrifugation in 1 ml of PBS-EDTA. (cdc.gov)

Marrow2

• Donor marrow was depleted of lymphocytes using counterflow centrifugation. (ashpublications.org)
• In bone marrow transplantation for leukemia, counterflow centrifugation is a useful technique for the prevention of GVHD. (ashpublications.org)

Aqueous1

• Centrifugation produced layers of an aqueous phase (water) on the bottom, an emulsion phase (cream) in the middle and an oil phase on top. (scialert.net)

Emulsion3

• In this study, the potential of centrifugation method in demulsification (emulsion breaking) of coconut milk emulsion is investigated. (scialert.net)
• The formulations were then evaluated microscopically, at 400 X magnification, when prepared with a lipid emulsion, and macroscopically after centrifugation at 6390 g. (usp.br)
• The preparations without a lipid emulsion were evaluated macroscopically under the same conditions and also after being kept at temperatures of 5 + 3oC, 24 + 3oC and 37oC for 96 hours. (usp.br)

Physiological conditions1

• their enzymatic repertoire is highly variable among species, individual cell types and under different physiological conditions. (biorxiv.org)

Tubes2

• Pick 40 islets per treatment or condition of interest into 1.5 milliliter tubes of islet medium. (jove.com)
• To address this situation, CHOPIN Technologies developed an automated system, based on the concepts of the AACCI standard method, but aiming to eliminate all potential impacts of the operator on the test and standardize tubes and centrifugation conditions. (usda.gov)

Extraction2

• The different factors that could affect the extraction capability were studied and optimized, including the type of double extractant and its volume, salt addition, vortex time, and centrifugation time. (bvsalud.org)
• Under optimum extraction conditions, this method provides high enrichment factors (EFs) of 73-318 with low limits of detection (LODs) of 0.3-1 µg L-1 and limits of quantitation (LOQs) of 1-3 µg L-1. (bvsalud.org)

Time4

• The centrifuge speed was varied from 6000 to 12000 rpm and the centrifugation time was varied from 30 to 105 min. (scialert.net)
• The present research found that, centrifugation method can enhance the demulsification of oil-in-water (O/W) coconut milk emulsions in a very short time compared to the conventional methods. (scialert.net)
• The four bottles placed inside a high speed centrifuge (Sorvall Evolution RC) with rotor used is SLA-1000 and centrifuged for different speeds ranging from 6000 to 12000 rpm (the angular velocities ω of the rotor ranging from 628.32 to 1256.64 rad sec -1 ) and different times ranging from 30 to 105 min, all experiments were run at 30°C. Same coconut milk used on each speed and centrifugation time. (scialert.net)
• QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour. (qiagen.com)

Cells6

• The dual-input Mother Machine (DIMM) chip enables controlled and continuous variation of external conditions, allowing direct observation of gene regulatory responses to changing conditions in single cells. (nature.com)
• Red blood cells are selectively lysed and white cells collected by centrifugation. (qiagen.com)
• White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. (qiagen.com)
• QIAfilter Cartridges , provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. (qiagen.com)
• Separate serum from cells immediately by centrifugation and aliquot into a polypropylene or similar plastic tube. (nationaljewish.org)
• After the second centrifugation, dissociate the islets into single cells with 125 microliters of 0.25%trypsin plus inhibitor for three minutes at 37 degrees Celsius with periodic gentle pipetting. (jove.com)

Selectively1

• The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. (qiagen.com)

Procedure1

• Repeat this procedure two more times using the same centrifugation conditions. (cdc.gov)

High3

• Featuring a high heating surface of up to 1,400 m 2 , the Cail & Fletcher massecuite reheater is a simple and efficient device for conditioning low-grade massecuite prior to centrifugation. (fivesgroup.com)
• A seven metres thick peat bog (Misten, Hautes-Fagnes, Belgium) has been studied at high resolution in order to reconstruct the conditions of peat formation and evolution on the basis of pollen, testate amoebae analysis, and geochemistry. (ac.be)
• Pretransplant conditioning consisted of cyclophosphamide and fractionated total body irradiation (TBI) with a low (4.1 +/- 0.3 cGy/min) or high (13.1 +/- 1.6 cGy/min) midline average dose rate. (ashpublications.org)

Method2

• Centrifugation is also the most common method used for uranium enrichment, relying on the slight mass difference between atoms of U-238 and U-235 in uranium hexafluoride gas. (wikipedia.org)
• The authors conclude that batch washing with modern equipment within the conditions used in the present experiment is an effective method of washing and keeps dust and endotoxin at acceptable levels. (cdc.gov)

Equivalent2

• Purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications. (qiagen.com)
• Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. (qiagen.com)

Medium3

• Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. (wikipedia.org)
• We followed up this analysis by showing that loss of cln5 alters the intracellular and extracellular amounts of proliferation repressors during growth and increases the extracellular amount of conditioned medium factor, which regulates cAMP signalling during the early stages of development. (frontiersin.org)
• Chondrocytes from OA patients were used in primary culture and stimulated with 10 ng/ml interleukin(IL)-1β in the presence or absence of AD-MSC microvesicles, exosomes or conditioned medium. (karger.com)

Processes1

• Despite their higher unit cost, organic polymers have largely displaced inorganic chemicals in conditioning and dewatering processes ( Chitikela & Dentel 1998 ). (iwaponline.com)

Plasma1

• The antibody in the recipient's serum or plasma is detected when the sensitized donor RBCs react with the anti-immunoglobulin G (IgG) in the microtube during centrifugation. (medscape.com)

Blood1

• Additionally, neuroprotectants could mitigate the ischemia/reperfusion injury, including edaravone and fingolimod, 3 but their clinical effects cannot achieve the desired condition, primarily due to the rigid blood-brain barrier (BBB) that limits drug delivery to the ischemic regions. (cbinsights.com)

Volume1

• The particles' settling velocity in centrifugation is a function of their size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. (wikipedia.org)

Visit1

• For additional information, visit ProSci's Terms & Conditions Page . (prosci-inc.com)

Standard1

• Â The only elements of the '858 patent family missing from Prevost's explicit teachings are specific pH, moisture content and temperature range requirements that are indisputably encompassed by the standard operating conditions of a dry mill ethanol plant and the heating element recited in some of the claims. (greenpatentblog.com)

Factor1

• a rough guide to the 'g' force exerted at this speed is to multiply the centrifugation radius by a factor of 10, so a radius of 160 mm gives approximately 1600 x g. (wikipedia.org)

Potential1

• This study further investigates the potential of dual-conditioning of sludge using the non-toxic and biodegradable biopolymer chitosan to replace synthetic polymers, together with some cheaper metal cations as an economical and eco-friendly dewatering alternative. (iwaponline.com)

Equipment1

• The equipment reduces massecuite viscosity for optimal centrifugation and throughput. (fivesgroup.com)

Present1

• We conclude that sodium glycerophosphate, when added to parenteral nutrition formulations together with calcium gluconate in the amounts and under the conditions tested here did not present in vitro incompatibility. (usp.br)

Effects1

• Cations may have significant effects on sludge conditioning by neutralization of negative surface charges, bridging of floc components, and the salting out effect, leading to improved dewaterability when used in conjunction with chitosan. (iwaponline.com)

Patients1

• In 43 patients, cytosine-arabinoside or anthracyclines were added to the conditioning regimen. (ashpublications.org)

Fresh2

• Invert the tube and collect the islets by centrifugation for a second wash in fresh PBS plus deubiquitinase inhibitor. (jove.com)
• Collect the islets by centrifugation for two washes in fresh PBS plus inhibitor per wash. (jove.com)

Storage Conditions1

• Under proper storage conditions the shelf life is half a year from the date of receipt. (prosci-inc.com)

Environmental2

• Second, measuring gene regulatory responses requires the ability to accurately control and vary environmental conditions. (nature.com)
• Vials are stored under appropriate frozen (-20°C) conditions until they are shipped to National Center for Environmental Health for testing. (cdc.gov)

Modern1

• Grade 52 Texas cotton was washed in a modern facility by the following process: mechanical opening and cleaning, prewetting and cakemaking, kier washing and rinsing, centrifugation and drying and baling. (cdc.gov)

Plant1

• It is available in three different designs (Cail, Rain Tray and Petal) to accommodate various plant conditions. (fivesgroup.com)