• The present study characterized hydrosalpinx epithelial cell culture and examined the effects of its conditioned medium (CM) on sperm motility, acrosome reaction and embryo development. (hku.hk)
  • Methods: Normal Fallopian tubes (n = 6) and hydrosalpinges (n = 9) were used to prepare epithelial cell culture and CM. Epithelial cell characterization was confirmed using electron microscopy. (hku.hk)
  • The established epithelial cell culture system may provide a model to further investigate the mechanisms underlying the toxic effects of HF on embryo development and the adverse effects on IVF outcomes. (hku.hk)
  • Caco-2 (Human epithelial colorectal adenocarcinoma cells, ATCC HTB-37) cells were cultured in Minimum Essential Medium (MEM) supplemented with NEAA (1x), P/S (100 U/mL), HEPES buffer (10 mM), sodium pyruvate (1 mM), and 20% (v/v) FBS. (frontiersin.org)
  • Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
  • SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)
  • The cells were cultivated in a mineral medium containing soluble starch as sole carbon source. (scielo.br)
  • In conclusion, Vero cells were grown on Cytodex 1 with serum-free media and a high amount of rabies virus was obtained. (researchsquare.com)
  • Adherent Vero cells can be used in different kinds of microcarriers for industrial production in cell culture type bioreactors. (researchsquare.com)
  • ATCC) cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with non-essential amino acids (NEAA, 1x), penicillin/streptomycin (P/S, 100 U/mL), HEPES buffer (10 mM) and 10% (v/v) Fetal bovine serum (FBS). (frontiersin.org)
  • A clinical isolate hCoV-19/Italy/UniSR1/2020 (GISAID accession ID: EPI_ISL_413489) was isolated and propagated in Vero E6 cells, and viral titer was determined by 50% tissue culture infective dose (TCID 50 ) and plaque assay for confirming the obtained titer. (frontiersin.org)
  • An aliquot (0.8 mL) of the transport medium of the nasopharyngeal swab (COPAN's kit UTM® universal viral transport medium-COPAN) of a mildly symptomatic SARS-CoV-2 infected patient was mixed with an equal volume of DMEM without FBS and supplemented with double concentration of P/S and Amphotericin B. The mixture was added to 80% confluent Vero E6 cells monolayer seeded into a 25 cm 2 tissue culture flask. (frontiersin.org)
  • In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. (elifesciences.org)
  • Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
  • Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
  • At each passage, the cells were counted, photographed using an inverted phase-contrast microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA) and cyro-preserved in cyrovial tube for further culture and later for RNA analysis. (bvsalud.org)
  • After washing, 1x10ˆ6 cells were seeded and cultured in KGM (Lonza Group Ltd., Basel, Switzerland). (bvsalud.org)
  • Total RNA was extracted from the cells cultured in KGM after 1, 3 and 7 days. (bvsalud.org)
  • For swab specimens, anaerobic transport media are available. (msdmanuals.com)
  • LabX.com is the premier marketplace for buying and selling, new, used and refurbished Cell Culture Media and Reagents and other laboratory equipment. (labx.com)
  • Nonculturable organisms do not reproduce in the laboratory because of intracellular stress or because the conditions (e.g., culture medium or incubation temperature) are not conducive to growth. (cdc.gov)
  • As the name implies, viable bioaerosol sampling involves collecting a bioaerosol and culturing the collected particulate. (cdc.gov)
  • When sampling for culturable bacteria and fungi, the bioaerosol is generally collected by impaction onto the surface of a broad spectrum solid medium (agar), filtration through a membrane filter, or impingement into an isotonic liquid medium (water-based). (cdc.gov)
  • SHED was cultured and maintained in T25 cm2 culture flask using Minimum Medium Alpha (α-MEM) from Gibco, USA. (bvsalud.org)
  • We found that 20 (10.6%) of 188 patients with chronic sup- nities, and P. aeruginosa was the predominant species in purative otitis media in Angola were co-colonized with fluo- 10 (50%) of the patients. (cdc.gov)
  • Some During studies of ear disharge caused by otitis media, patients in the geographic area also used cockroach paste, we detected the gram-negative bacillus Alcaligenes faeca- palm oil, sweet olive oil, sewing machine oil, or breast lis in addition to the commonly isolated bacterial species milk to prevent ear discharge. (cdc.gov)
  • In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. (ejbiotechnology.info)
  • Some fungi, particularly molds (eg, Aspergillus species), usually cannot be cultured from blood. (msdmanuals.com)
  • For most of these conditions, the diagnosis can be ascertained through clinical history and presentation, but, on occasion, biopsy may be necessary. (medscape.com)
  • The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the mineral medium shortened the lag period and improved the growth and alpha-amylase synthesis. (scielo.br)
  • The strain, grown in the medium with 1-2% chicken feathers and a yeast extract supplement, produced keratinases of 32 KU and lower level of proteases, 6 PU. (scielo.br)
  • TOA-1, grown on a skim milk/yeast extract medium, biosynthesized a highly stable keratinolytic enzyme with an optimum activity at pH 11.0-11.5 and temperature of 70-75 °C. This 20 kDa serine protease exhibited a high specific activity towards keratin and lower towards casein. (scielo.br)
  • The optimum temperature and initial medium pH for amylase synthesis by the organism were 50°C and 7.0 respectively. (scielo.br)
  • Blood cultures require decontamination and disinfection of the skin (eg, povidone iodine swab, allowed to dry, removed with 70% alcohol). (msdmanuals.com)
  • The application of in vitro propagation techniques may offer the possibility of producing large number of uniform plants for breeding programs and further field culture. (ejbiotechnology.info)
  • The produced keratinolytic enzymes were mainly a combination of alkaline serine or thiol proteases, active at the optimum pH 9.4, 55 °C. Four main protease fractions of 62, 185, 139 and 229 kDa were identified in the crude culture fluid. (scielo.br)
  • Results: The percentage of human motile sperm incubated in hydrosalpinx CM was significantly different from those in normal Fallopian tube (NFT) CM and modified human tubal fluid medium (hTF) (control) (P (hku.hk)
  • The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase, demonstrating that a classical glucose effect is operative in this organism. (scielo.br)
  • The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase. (scielo.br)
  • however, TSP‑1 and ADAMTS‑1 levels did not differ between high glucose and normal (5 mM) glucose conditions. (spandidos-publications.com)
  • The results of this study suggest that kaempferol targets ERRα and suppresses the angiogenesis of HRECs under high glucose conditions. (spandidos-publications.com)
  • We tested A. faecalis isolates against a series of select- hronic suppurative otitis media (CSOM) is a common ed antimicrobial drugs by using broth microdilution (on- condition in developing countries and in original pop- line Technical Appendix Table) and found 100% were sus- ulations such as the Inuit ( 1 ). (cdc.gov)
  • Topical treatment using antimicrobial drugs in combi- in patients who had acute otitis media, peritonitis, and eye nation with keeping the ear canal clean and dry is the main- or urinary tract infections ( 4-6 ). (cdc.gov)
  • Culture also facilitates testing of antimicrobial susceptibility. (msdmanuals.com)
  • Culturable organisms reproduce under controlled conditions. (cdc.gov)
  • Cultures from infected catheters generally turn positive more quickly and contain more organisms than simultaneously drawn peripheral blood cultures. (msdmanuals.com)
  • Cell infection, at an MOI of 0.3 with serum-free medium conditions, yielded a maximal rabies virus titer of 1.82×10 7 FFU/mL at 5 days. (researchsquare.com)
  • Calu-3 (Human lung cancer cell line, ATCC HTB-55) were cultured in MEM supplemented with NEAA (1x), P/S (100 U/mL), HEPES buffer (10 mM), and 10% (v/v) FBS. (frontiersin.org)
  • The specimen must be transported rapidly, in the correct medium, and in conditions that limit growth of any potentially contaminating normal flora. (msdmanuals.com)
  • The aim of this study was to detect the expression of molecules associated with Notch signallng pathway in SHED cultured in specific differentiation medium, namely, KGM. (bvsalud.org)
  • 1,3-DNB can undergo biodegradation under aerobic and anaerobic conditions in water and soil. (cdc.gov)
  • However, the foundations of a political culture necessary to consolidate liberal democracy are still weak in most African states. (lu.se)
  • 1994). This pattern of localized toxicity emphasizes the impor- inhaled organic acid vapor, acrylic acid, than the olfactory epi- tance of local tissue dose for interspecies extrapolation and risk thelium of the rodent nasal cavity when the exposure conditions assessment. (cdc.gov)
  • The bulblets at the end of the culture period were given cold treatment at 4ºC for 2-8 weeks at a 2-weeks interval and then transplanted to a potting mixture of sand, leaf mold and peat moss (1:1:1 v/v). The best emergence rate (90%) was achieved at 8 weeks cold treatment for winter harvested bulbs. (ejbiotechnology.info)
  • For the purpose of the definitive test, the test item was dissolved directly in culture medium. (europa.eu)
  • Sample storage conditions before analysis: 50mL test solution were extracted three times with ethyl acetate (15 mL per extraction). (europa.eu)
  • Sample storage conditions before analysis: Duplicate samples were taken and stored at approximately - 20°C for further analysis if necessary. (europa.eu)
  • When sampling is indicated, it is advisable to sample before, during, and after the sampling area is occupied, including times when the heating, ventilating, and air conditioning system is activated and inactivated. (cdc.gov)
  • Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. (bvsalud.org)
  • THe pH range narrowed as the media aged, because the test media slowly equilibrated with atmospheric carbon dioxide due to aeration. (europa.eu)
  • Find Cell Culture Media and Reagents that are available to purchase online, or send a quote request to the vendor directly. (labx.com)
  • Find informative news, articles, product insights and comparisons about Cell Culture Media and Reagents in our News & Insights area. (labx.com)
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  • All cell culture conditions and virus growing kinetics in serum-free media were investigated. (researchsquare.com)
  • The cell culture-based rabies vaccine was established in 1967. (researchsquare.com)
  • This is because the physical-chemical nature of many lignocellulosic substrates naturally lends itself to solid phase culture, and thereby represents a means to reap the acknowledged potential of this fermentation method. (mdpi.com)
  • Information regarding environmental conditions and media to culture microorganisms is shown in Sections 3.a. and 3.c. (cdc.gov)
  • Hydrolysis is not expected to be an important fate process since aromatic nitro compounds are generally resistant to chemical hydrolysis under environmental conditions. (cdc.gov)
  • With technological advances in radio, television and cinematography in subsequent decades, the importance of entertainment media in ideological, social and moral proselytizing became more pronounced. (theinterim.com)
  • Recent studies have also shown a correlation between social media use and narcissistic tendencies. (articleinsider.com)
  • The constant need for validation and attention on social media platforms can reinforce narcissistic behavior and exacerbate existing narcissistic traits. (articleinsider.com)
  • Africa has seen a renewed quest for democracy expressed itself in the streets, popular culture, the Internet, and social media. (lu.se)
  • Therefore the pH of the fresh media increased slightly at higher test concentrations. (europa.eu)
  • Fresh sterile culture medium (100 mL) was added to each to ensure that the test concentration was reduced to below the inhibiting level. (europa.eu)
  • The upstream process was carried out in an animal component-free medium on Cytodex 1 microcarriers. (researchsquare.com)
  • Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): Not applicable. (europa.eu)
  • should not be cultured from sites where they are part of the normal flora because differentiation of pathogens from normal flora may be impossible. (msdmanuals.com)
  • This has left it up to the news media to convey some sense of the reality of armed conflict. (foreignpolicy.com)
  • Balanced salt solutions for medium preparation. (quidel.com)
  • Aliquots were removed from each replicate control and test culture (0.25 mL and 0.50 mL respectively) and the replicates pooled. (europa.eu)