• However, this SOCS3 fusion proteins expressed in bacteria cells were hard to be purified in soluble form. (freepatentsonline.com)
  • Background: The purpose of this study was to compare plasma soluble endoglin (sEng) levels with standard clinical evaluation or plasma levels of other angiogenic proteins [soluble fms-like tyrosine kinase 1 (sFlt1) and placental growth factor (PlGF)] in predicting short-term adverse maternal and perinatal outcomes in women with suspected preeclampsia presenting prior to 34 weeks. (harvard.edu)
  • We investigated the impact of RAGE suppression by soluble RAGE (sRAGE) on the angiogenic response in a diabetic hindlimb ischemia mouse model. (hindawi.com)
  • The migration and activation of leucocytes within the airways may be the consequence of soluble proteins released by resident cells that are activated by various stimuli. (biomedcentral.com)
  • The angiogenic response involved in wound repair results from the stimulation of endothelial cells by growth factors [including acidic (aFGF) and basic fibroblast growth factor (bFGF)] to migrate, proliferate, and form the endothelial tubes and capillary structures that undergo transformation into capillary vessels and lead to the restoration of the microvascular network ( 1 , 13 , 21 ). (physiology.org)
  • To determine whether human retinal endothelial cells (HRECs) express the endothelial cell protein C receptor (EPCR) and to realize its potential as a targeting moiety by developing novel single and dual corticosteroid-loaded functionalized liposomes that exhibit both enhanced uptake by HRECs and superior biologic activity compared to nontargeting liposomes and free drug. (arvojournals.org)
  • MC extracts or low concentrations of bovine serum albumin-derived AGEs (BSA-AGEs) stimulated proliferation, migration (using wound-healing assay) and tube formation (using Matrigel™-embedded 3D culture) of bovine aortic endothelial cells (BAEC) together with increases in the phosphorylation of extracellular signal-regulated kinase (ERK)1/2, the key angiogenic signaling cytoplasmic protein. (rsc.org)
  • However, systemic protein delivery in vivo has been proven difficult due to poor tissue penetration and rapid clearance. (freepatentsonline.com)
  • BD Matrigel Matrix HC developed by BD Biosciences can be utilized to evaluate in vivo angiogenic activity of various compounds by subcutaneous injection into mice (BD Matrigel Plug Assay). (news-medical.net)
  • 01) was found between the extent of vascularization of the bone marrow of a given patient and the angiogenic activity exerted in the CAM assay by the plasma cells isolated from the same bone marrow. (bloodjournal.org)
  • All patients were sampled twice before receiving treatment and the mean values of plasma protein concentration of the several CAF were used, in order to reduce the impact of intra-patient and assay variability. (esmo.org)
  • The SearchLight protein array was the assay used in this last step. (esmo.org)
  • In a new study conducted on human tissues and in chicken eggs, low levels of blood vessel forming proteins in schizophrenic patients are identified as probable cause for schizophrenia. (visembryo.com)
  • Previously developed hydrophobic CPPs-named membrane translocating sequence (MTS), membrane translocating motif (MTM) and macromolecule transduction domain (MTD)-are able to deliver biologically active proteins into a variety of cells and tissues. (freepatentsonline.com)
  • 4-6 High concentration of AGEs affect tissues and cause various adverse cellular events, including increased free radical activity, which can damage cell membranes and promote mutations, alterations in enzymatic activity, cross-linking and impaired degradation of proteins. (rsc.org)
  • We aimed to elucidate the role of these angiogenic factors in placentation and to evaluate the predictive value of their protein concentrations and genetic variations in pregnancy complications.METHODSWe performed a systematic search of PubMed, and retrieved original articles. (biomedsearch.com)
  • In this paper, Tran et al assessed the prognostic and/or predictive value of several cytokines and angiogenic factors (CAF) in mRCC treated with Pazopanib, using peripheral blood samples from patients who participated in the phase II (single arm) and III (randomised, placebo-controlled) clinical trials. (esmo.org)
  • Maternal Serum Concentrations of the Chemokine CXCL10/IP-10 Are Elevated in Acute Pyelonephritis During Pregnancy The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians. (jove.com)
  • Previously, recombinant proteins consisting of suppressor of cytokine signaling 3 (SOSC3) fused to the fibroblast growth factor (FGF) 4-derived MTM were developed to inhibit inflammation and apoptosis. (freepatentsonline.com)
  • IRESs, RNA elements that permit cap-independent translation, were first discovered in viral RNAs but have also been found in several cellular mRNAs mostly encoding control proteins such as transcription factors, growth factors or proteins involved in apoptosis [ 14 - 16 ]. (biomedcentral.com)
  • These recombinant SOCS3 proteins fused to aMTD/SD having much higher solubility/yield and cell-/tissue-permeability have been named as improved cell-permeable SOCS3 (iCP-SOCS3) proteins. (freepatentsonline.com)
  • The micro-environment affects the outcome of angiogenic responses in patients with diabetes mellitus. (rsc.org)
  • Epithelial cells were exposed to proteins produced by nerve cells from the schizophrenic brains. (visembryo.com)
  • Thus, MC extracts exert direct pro-angiogenic signaling mediated via RAGE to overcome the anti-angiogenic effects of high BSA-AGEs, highlighting the biphasic RAGE-dependent mechanisms involved. (rsc.org)
  • This study enhances our understanding of the mechanisms underlying the pro-angiogenic effects of MC extracts in improvement of diabetes-impaired wound-healing. (rsc.org)
  • 01) levels of the angiogenic basic fibroblast growth factor (FGF)-2 in the active MM patients than in nonactive MM and MGUS patients (153 ± 59, 23 ± 17, and 31 ± 18 pg FGF-2/100 μg of protein, respectively). (bloodjournal.org)
  • Significant changes in levels of 21 proteins were observed over time. (readbyqxmd.com)
  • A novel protein array technology, which can simultaneously and quantitatively measure few protein levels from hundreds and thousands of samples was developed. (aacrjournals.org)
  • The ability of these methods to quantitatively measure multiple samples for angiogenic factors simultaneously is greatly limited because they require large sample volumes and exhibit low throughput. (aacrjournals.org)
  • Neural cells made from the skin cells of patients with schizophrenia, produced smaller amounts of two proteins essential in making blood vessels. (visembryo.com)
  • LTB-4 concentrations are high in blood and pleural effusion [ 19 ] but have not as yet been measured in other airway samples of lung cancer patients. (biomedcentral.com)
  • This is paralleled by an increased angiogenic and invasive potential of bone marrow plasma cells, which is dependent, at least in part, by FGF-2 and MMP-2 production. (bloodjournal.org)
  • In principle, protein-based biotherapeutics offers a way to control biochemical processes in living cells under non-steady state conditions and with fewer off-target effects than conventional small molecule therapeutics. (freepatentsonline.com)
  • Seven human cancer cell lines grown in glucose plus acetoacetate medium showed tightly coupled reduction of growth and ATP concentration. (biomedcentral.com)
  • We illustrate the power of label-free LC-MS/MS quantification by considering four examples: complexity of eosinophil STATs, contribution of immunoproteasome subunits to eosinophil proteasomes, complement of integrin subunits, and contribution of platelet proteins originating from platelet-eosinophil complexes to the overall proteome. (readbyqxmd.com)
  • In total 2501 proteins were accurately quantified in 3 biological replicates on at least 1 of the 7 different time-points analyzed. (readbyqxmd.com)
  • 11,12 Many studies have described both pro- and anti-angiogenic effects of AGEs, with effects that vary with the concentrations and types of AGEs. (rsc.org)
  • Treatment with C225 plus gemcitabine resulted in additive cytotoxic effects that increased with increasing gemcitabine concentrations. (aacrjournals.org)
  • Flushing out a complete profile of vitreous proteins involved across a spectrum of ischemic retinopathies, including neovascular glaucoma (NVG), may serve to direct future research and therapies. (arvojournals.org)
  • There is also a rapid and unbiased RT-quantitative PCR method to investigate the angiogenic developments in the Matrigel plug in response to fibroblast growth factor-2. (news-medical.net)