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Our results indicated that terpinen-4-ol elicited a dose-dependent cytotoxic effect, as determined by MTT assay. (hindawi.com)

In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
In this investigation, the role of autophagy and apoptosis in Solanum nigrum fruit extract-induced cytotoxicity in MDA-MB-468, a triple negative breast cancer cell line was studied. (ijpsonline.com)
The absorbances averages results obtained showed that the crude extract did not present cytotoxicity for the HTC cells in all the concentrations and evaluated times. (scielo.br)
Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC). (scielo.br)
Bortezomib increased the cytotoxicity of cisplatin and pemetrexed in a concentration-dependent manner when administered prior to either. (mesothelioma-line.com)
Methods used to study the cytotoxic effects include cytotoxicity assay, double staining cell morphological analysis, cell cycle analysis, apoptosis test and cell proliferation assay. (upm.edu.my)
To investigate the mechanism of EESR-mediated cytotoxicity, HT29 cells were treated with various concentrations of EESR and the induction of cell cycle arrest and apoptosis was analyzed by flow cytometry, 4,6-diamidino-2-phenylindole staining, and Western blot analysis. (jcpjournal.org)
In a study published in the Journal of Medicinal Plants Research , Brazilian researchers investigated the cytotoxicity of various Passiflora species using colon cancer, ovarian cancer and leukemia cell lines. (naturalnews.com)
They then evaluated the in vitro cytotoxicity of these extracts against cancer cell lines using MTT assay. (naturalnews.com)
Among the Passiflora leaf extracts evaluated, ELPA exhibited the highest cytotoxicity against HL-60 cells (IC50 19.37 u g/mL) but was not cytotoxic against PBMC, suggesting selectivity for cancer cells. (naturalnews.com)
Our results indicate that the events associated with paclitaxel-induced cytotoxicity are connected to each other and represent the signaling network of paclitaxel-induced mitotic arrest and cell death. (johnshopkins.edu)
Thus, the present study reports nanoscale quantitative structure-activity relationship (nano-QSAR) models, which are based on an ensemble learning approach, for predicting the cytotoxicity of heavy metals adsorbed on nano-TiO 2 to human renal cortex proximal tubule epithelial (HK-2) cells. (beilstein-journals.org)
RESULTS: Starting from the concentration of 0.31 mg/ml, Hemidesmus indicus induced cytotoxicity and altered cell-cycle progression, through a block in the G0/G1 phase. (unibo.it)
L. egeregia was regarded as the prototype of the anticancer species due to its profound flavonoid concentration (85.40 µg/mL) and cytotoxicity (9.46 µg/mL) compared to other extracts. (who.int)

Abstract: BC-11 is an easily synthesized simple thiouronium-substituted phenylboronic acid, which has been shown to be cytotoxic on triple negative MDA-MB231 breast cancer cells by inducing a perturbation of cell cycle when administered at a concentration equal to its ED50 at 72 h (117 µM). (figshare.com)
In recent studies, this linker modified analog of goniothalamin was shown to be cytotoxic against PC-3 (prostate cancer) and MCF-7 (human breast cancer) cell lines with half maximal inhibitory concentration values of 0.13 Î¼M and 2.6 Î¼M, respectively. (lookformedical.com)

Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
They then conducted morphological analyses using light and fluorescence microscopy, and cell cycle and DNA fragmentation analyses using flow cytometry to study the mechanism of cell death induced by ELPA in HL-60 cells. (naturalnews.com)
Cell cycle analyses were performed using flow-cytometry and multicycle analysis software (Phoenics Flow Systems, San Diego, CA, USA). (niom.no)
The cell apoptosis was detected using flow cytometry. (lookformedical.com)
Induction of apoptosis, cell-cycle analysis, levels of specific membrane differentiation markers were evaluated by flow cytometry. (unibo.it)
Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
Expression of chemoresistance molecules was evaluated using flow cytometry in U87MG and human umbilical vein endothelial cells (HUVECs). (biomedcentral.com)

The study aim was to evaluate the cytotoxic activity, using the MTT test [3-(4,5-Dimethilthiazol-2-yl)-2,5-diphenil tetrazolium bromide], from the crude extract of Picrasma crenata (Pau Tenente) and its isolated compounds, quassin and parain, in culture of rat liver tumor cells (HTC). (scielo.br)
Therefore, the aim of this study was to evaluate the cytotoxic and anti-proliferative properties of Cannabis sativa and its isolate, cannabidiol in cervical cancer cell lines. (biomedcentral.com)

Based mostly upon the alkaline comet assay extracts had been discovered to induce genotoxicity at concentrations starting from 0.437 to 7 μg/ml. (esb-itathematic.it)
Additional, enzyme-modified comet assay confirmed each oxidized purines and pyrimidines, and consequently oxidative stress was associated to genomic instability and cell demise. (esb-itathematic.it)
Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. (jcpjournal.org)
Changes in density of viable cells after exposure were measured using the MTT assay. (niom.no)
HPMA exposure resulted in reduced SDH activity (MTT assay), altered cell growth pattern and increased cell death in a dose dependent manner in all investigated cell lines. (niom.no)
First, the sensitivity and IC 50 values of chidamide and bortezomib in several gastric cancer cell lines (MGC-803, BGC-823, SGC-7901, and MKN45) were measured using the CCK-8 assay. (dovepress.com)
dCK activity of the cells was determined by an enzyme activity assay. (biomedcentral.com)
The half-maximal inhibitory concentration (IC50) of Cd and the safe doses of SFN were determined using a methyl thiazolyl tetrazolium (MTT) assay. (lookformedical.com)
The testosterone secretion from TM3 cells was measured using the enzyme-linked immunosorbent assay. (lookformedical.com)
To investigate the putative anti-proliferative effect, apoptosis assay, microRNA expression profile and study of cell cycle were performed. (biomedcentral.com)

Bortezomib was highly cytotoxic to MPM cells and induced both G(2)/M and G(1)/S cell cycle arrest. (mesothelioma-line.com)
These compounds proved to be highly cytotoxic to the leukemia cell line HL-60 but not to normal cancer cells, suggesting a selectivity to cancer cells that could prove beneficial in clinical settings. (naturalnews.com)

Vanillin also induced apoptosis and cell cycle arrest. (upm.edu.my)

Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
Using NSCLC cell lines with differential EGFR status, we examined the potency of PA-MSHA (Pseudomonas aeruginosa-mannose-sensitive hemagglutinin) in combination with Gefitinib on proliferation, apoptosis, cell cycle arrest, EGFR signaling and tumor growth. (oncotarget.com)
The effects of the drugs on proliferation, cell cycle distribution and apoptosis were evaluated. (oncotarget.com)
Previous studies have demonstrated that glycolysis inhibitors reduce the proliferation of non‑small cell lung cancer (NSCLC) cells by interfering with the aerobic glycolytic pathway. (spandidos-publications.com)
RFC4 knockdown induced G2/M cell cycle arrest and inhibited NPC cell proliferation in vitro and in vivo . (engineering.org.cn)
Interestingly, HOXA10 was confirmed as a downstream target of RFC4, and the overexpression of HOXA10 attenuated the silencing of RFC4-induced cell proliferation, colony formation inhibition, and cell cycle arrest. (engineering.org.cn)
For the first time, this study reveals that RFC4 is required for NPC cell proliferation and may play a pivotal role in NPC tumorigenesis. (engineering.org.cn)
In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. (medsci.org)
The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. (medsci.org)
EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. (jcpjournal.org)
6 , 7 Therefore, the inhibition of cancer cell proliferation by inducing cell cycle arrest and apoptosis is being an important preventive strategy against cancer. (jcpjournal.org)
Objective To explore the mechanism of Xiaoaiping injection enhancing the inhibitory effect of paclitaxel on the proliferation of ovarian cancer cell A2780. (yydbzz.com)
The inhibition ratios of different drugs on the proliferation of A2780 cells were measured by MTT method. (yydbzz.com)
Results Xiaoaiping injection inhibits the proliferation of A2780 cells in a time- and concentration-dependent manner. (yydbzz.com)
Compared with paclitaxel treatment alone, Xiaoaiping injection combined with paclitaxel exhibit more significant inhibitory effect on A2780 cell proliferation RT-PCR and Western blotting results revealed that Xiaoaiping injection can significantly inhibit paclitaxel-induced over expression of PXR and CYP2C8. (yydbzz.com)
Conclusion Xiaoaiping injection can significantly enhance the inhibitory effect of paclitaxel on the proliferation of ovarian cancer A2780 cells. (yydbzz.com)
Then, the relatively insensitive gastric cancer cell lines (MGC-803 and BGC-823) were treated with low concentrations of chidamide alone, bortezomib alone, or chidamide and bortezomib combination to detect the effects on cell proliferation, apoptosis, migration, and invasion. (dovepress.com)
Compared with low-dose chidamide or bortezomib alone, the low-dose drug combination significantly inhibited the proliferation, migration, and invasion of MGC-803 and BGC-823 cells and induced apoptosis of the cells. (dovepress.com)
Our results suggest that the combination of chidamide and bortezomib can significantly reduce the proliferation, invasion, and migration of MGC-803 and BGC-823 cells, providing a framework for the clinical evaluation of combined therapies for gastric cancers. (dovepress.com)
Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. (biomedcentral.com)
These compounds are almost structurally similar or possess identical pharmacological activities and offer various potential applications including the ability to inhibit cell growth, proliferation and inflammation [ 22 ]. (biomedcentral.com)

RESULTS: The half maximal inhibitory concentration (IC50) of quercetin against dengue virus was 35.7 mug mL-1 when it was used after virus adsorption to the cells. (who.int)
IC50 or half maximal inhibitory concentration is a measure of the effectiveness of a substance in inhibiting a biochemical function. (naturalnews.com)
Interestingly, the half maximal inhibitory concentration (IC50) of BLMs in different cancer cell lines varies from nM to μM ranges. (lookformedical.com)
HUVEC when comparing the IC50 (half maximal inhibitory concentration) values. (lookformedical.com)
After 72 h incubation, colorimetric 3-[4,5-dimethylthiazol-2- yl]-2,5 diphenyl tetrazolium bromide test was performed to determine half-maximal inhibitory concentration values of each compound. (ijpsonline.com)

These data suggest that topotecan needs to be administered in high concentrations, such as an intratumoral polymer, to limit glioma cell growth in synergy with CD95L in vivo . (aspetjournals.org)
In combination studies with cisplatin, bortezomib was generally synergistic at high concentrations and antagonistic at low concentrations. (mesothelioma-line.com)
However, vanillin must be administered at high concentrations and prevented from being oxidized by the upper gastrointestinal tract to be medically effective. (upm.edu.my)
The negative effect of oral and intra-peritoneal administration of vanillin to Sprague-Dawley rats in unoxidized form at high concentrations (150 mg/kg)and 300 mg/kg) was also investigated. (upm.edu.my)
It was not showing negative effects when administered at high concentrations through oral and intra-peritoneal injections. (upm.edu.my)

The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
Findings from the in vitro study showed that vanillin was cytotoxic towards HT-29 and 3T3 cells with the IC50 value of 400 μg/ml and 1000 μg/ml respectively. (upm.edu.my)
The intention of this research was to find out the chemical profile, cytotoxic and genotoxic results of ethanolic extracts of S. planicaulis collected in winter (leaf extract) and summer season (leaf extract and leaf + flower extract) utilizing an in vitro mannequin of human neuroblastoma cell line SH-SY5Y. (esb-itathematic.it)
Aim of this study was to evaluate possible in vitro cytotoxic effects of two new-generation antiepileptic drugs on a human glioma cell line U87MG. (biomedcentral.com)

However, the expressions of a few xenobiotic metabolism, cell cycle arrest and apoptosis genes were up-regulated by 5 % ethanol injection. (upm.edu.my)

These compounds inhibit cell mitosis by binding to the protein tubulin in the mitotic spindle and preventing polymerization or depolymerization into the microtubules. (wikipedia.org)
Anti-dengue activity of these compounds was determined at different stages of DENV-2 infection and replication cycle. (who.int)
Thus, the results demonstrate a first indication of the cytotoxic activity of compounds quassin and parain, adding an important social and economic value to them, and may have application in future research and in pharmaceutical industry. (scielo.br)
previously described oxidative stress-inducing compounds causing cell cycle delay led to further investigation in the involvement of equisetin and TA-289 in mitochondrial-mediated generation of reactive oxygen species. (vuw.ac.nz)
Similar effects observed with equisetin treatment suggest that both compounds share the same mechanism, eliciting cell death via ROS production in the mitochondrial respiratory chain. (vuw.ac.nz)
Our analyses show that there is a significant antiviral activity associated with proteins in the extract, although other compounds also seem to contribute to inhibiting the replication cycle of these viruses. (frontiersin.org)
Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
The metal complexes of the compounds had stronger cytotoxic effects and cleaved caspase-3 expression than their ligands. (ijpsonline.com)
Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
however, mechanisms through which these bioactive compounds act on epithelial cells have not been identified. (biorxiv.org)

CD95 ligand (CD95L)-induced apoptosis was synergistically enhanced by short-term/high concentration but not long-term/low concentration exposure to topotecan, suggesting that topotecan sensitizes human malignant glioma cells to CD95L-induced apoptosis via inhibition of RNA synthesis. (aspetjournals.org)
However, in recent years, there has been increasing evidence that metformin exerts its anticancer effects through the inhibition of oxidative phosphorylation (OXPHOS) of tumor cell mitochondria, and metabolic pathways based on metformin targeting have only recently become the focus of intensive research. (spandidos-publications.com)
Solanum nigrum (SN) is a herb with anticancer effects such as destruction of tumor cell membrane, induction of apoptosis by NF-kappaB, caspase activation, nitric oxide production and inhibition of angiogenesis [ 3 ]. (ijpsonline.com)
Cannabis sativa in particular cannabidiol, we propose it plays important role in helping the body fight cancer through inhibition of pain and cell growth. (biomedcentral.com)
Gyp-L-induced cell death was associated with lysosomal swelling and autophagic flux inhibition. (oncotarget.com)
The diphosphate (dFdCDP) and triphosphate (dFdCTP) forms of the drug play an important role in the cytotoxic effect: dFdCDP is an inhibitor of ribonucleotide reductase, while dFdCTP is incorporated into DNA, both leading to the inhibition of DNA synthesis. (biomedcentral.com)
particularly, the effect of miR-195-5p modulation seemed to affect cell cycle, while miR-107 seemed to be implicated in the inhibition of cells migration. (biomedcentral.com)

Previous studies showed that vanillin was a good red blood sickle cell inhibitor, anti-microbial agent and anti-mutagen. (upm.edu.my)
EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. (jcpjournal.org)
The ribonucleotide reductase inhibitor hydroxyurea exhibits potent synergism, even at low, non-cytotoxic concentrations, with the anti-HIV-1 dideoxynucleoside 2',3'-dideoxyinosine, bringing about failure of HIV DNA synthesis and, thus, of HIV replication. (tmu.edu.tw)
As far as LEV is concerned, in 2010 Bobustuc hypothesized that AED may modulate O-6 methylguanine-DNA methyltransferase (MGMT), a DNA repair protein that has an important role in tumor cell resistance to alkylating agents, and LEV was reported as the most potent MGMT inhibitor among several AED [ 10 ]. (biomedcentral.com)

In general, genes involved in stress response, cell cycle control, drug metabolism, apoptosis and growth were found differentially expressed in the Cr(VI) exposed fibroblasts. (cdc.gov)
The expression levels of autophagy-related genes, Lc3 and Beclin1 increased in cells treated with 1.5 mg/ml of Solanum nigrum extract. (ijpsonline.com)
Further analysis with GenomeLab GeXP genetic system on brain tissues showed that the expression of most xenobiotic metabolism, cell progression, tumour suppressor, DNA damage and inflammation genes was maintained at normal level. (upm.edu.my)
Targeted therapy drugs are used to target specific genes and proteins of cancer cells to precisely identify and attack specific types of cancer cells. (medsci.org)
In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)

Selectivity Index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for each compound. (who.int)
The IC50 decreased to 28.9 mug mL-1 when the cells were treated continuously for 5 h before virus infection and up to 4 days post-infection. (who.int)
Daidzein showed a weak anti-dengue activity with IC50 = 142.6 mug mL-1 when the DENV-2 infected cells were treated after virus adsorption. (who.int)
The researchers also determined the IC50 of P. alata (ELPA) leaf extracts against both cancer (HCT-116, SF-295, OVACAR-8 and HL-60) cells and non-cancer cells (PBMC). (naturalnews.com)

RNA was extracted from colon for DNA repair, apoptosis, cell cycle, antiinflammation, proto-oncogene, colorectal cancer biomarker and tumor suppressor gene expressions study. (upm.edu.my)
The colon gene expression analysis showed that vanillin could enhance recombinational repair and mismatch repair, arrest cell at cell cycle checkpoints, increase the expression of tumour suppressor gene, colorectal cancer biomarker and proto-oncogene. (upm.edu.my)
In conclusion, vanillin could induce cytotoxic effects on colorectal cancer cells. (upm.edu.my)

Therapeutic approaches that target the metabolism of tumor cells have been a popular research topic in recent years. (spandidos-publications.com)
However, the mitochondrial oxidative phosphorylation (OXPHOS) pathway in tumor cells has also been implicated in lung cancer metabolism. (spandidos-publications.com)
Certain metabolic alterations, also referred to as metabolic reprogramming, are commonly observed in tumor cells and are proposed to be hallmarks of cancer ( 8 ). (spandidos-publications.com)
Given the vast differences in metabolism between healthy and tumor cells, there is hope that selective targeting of tumor metabolism may be achieved while limiting toxicity to healthy tissue. (spandidos-publications.com)
One of the hallmarks of breast cancer is resistance of tumor cells to cell death. (ijpsonline.com)
The product is a first-in-class aminopeptidase-targeting peptide-drug conjugate that rapidly delivers an alkylating payload into tumor cells. (oncopeptides.com)

Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)

Methods Ovarian cancer cell line A2780 were treated by Xiaoaiping injection (20,40,80,160 mg·mL -1 ) or paclitaxel(10,40,80,160 nmol·L -1 ) or Xiaoaiping injection combined with paclitaxel, respectively. (yydbzz.com)
Several factors hinder the identification of risk factors for central nervous system (CNS) involvement in diffuse large B-cell lymphoma (DLBCL), including the retrospective nature of most studies, the relatively low frequency of CNS relapse in DLBCL, and the heterogeneity of CNS prophylaxis methods used in these studies. (haematologica.org)

The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
The resistant mutant displayed a higher basal level of ROS production compared to the wild-type parent, indicating that the resistance mutation led to an up-regulation of antioxidant capacity which provides cell survival in the presence of TA-289. (vuw.ac.nz)
to counterbalance the production of ROS and protect the cells from oxidative damage, cells also possess a robust antioxidant system. (nhsjs.com)
Using cytotoxic and antioxidant studies, the study attempted to assess some of the commonly used medicinal plants used to cure cancer among Yoruba people in Ogun, Oyo, Osun, and Lagos (South-West, Nigeria). (who.int)
Samples of commonly utilized anticancer plants obtained from the chosen areas using physical and virtual oral seminars were studied for physiochemical composition and a possible antioxidant and cytotoxic potential to validate the basis for the use of the selected anticancer plants. (who.int)

Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
12 The activation of cyclin/CDK complex accelerates cell cycle progression, which are regulated by phosphorylation. (jcpjournal.org)

The generation of a mutant strain resistant to TA-289 also displayed resistance to a known oxidant, H2O2, at concentrations that were cytotoxic to wild-type cells. (vuw.ac.nz)

Ideally, a drug that inhibits PKB should cause both cell cycle arrest and promote apoptosis. (justia.com)
In fungi, ozone inhibits cell growth at certain stages. (silvermedicine.org)

A time-dependent and concentration-dependent cytotoxic effect of PA-MSHA was observed in all NSCLC cells tested. (oncotarget.com)
In the 1920s, Otto Heinrich Warburg discovered that cancer cells, unlike normal cells, use the glycolytic pathway to obtain energy for growth even in the presence of oxygen, which is a phenomenon known as 'aerobic glycolysis' or the Warburg effect ( 9 ), and aerobic glycolysis is a common metabolic phenotype in NSCLC ( 11 ). (spandidos-publications.com)
Different concentrations of Solanum nigrum extract showed cytotoxic effect on MDA-MB-468 cells. (ijpsonline.com)
We determined the effect of bortezomib on the cell cycle, apoptosis, and on the expression of cell cycle proteins p21/WAF1 and p27/KIP1 and on apoptosis-related proteins IAP-1, IAP-2, survivin, and XIAP. (mesothelioma-line.com)
From the in vivo study, results showed that treatment of 300 mg/kg of vanillin by intra-peritoneal injection caused the rats to be unconscious without exerting any negative effect on blood cells, kidney and liver. (upm.edu.my)
The present invention provides inhibitors of protein kinases comprising a molecule having at least a first moiety competent for penetration of the molecule into cells, and a second moiety for having a protein kinase inhibiting effect within the cells. (justia.com)
The aim of this study was to investigate the antitumor effect of chidamide in combination with bortezomib on gastric cancer cell lines. (dovepress.com)
Finally, the inhibitory effect of the combined chidamide and bortezomib treatment on MGC-803 cells was verified in vivo through tumor formation experiments in nude mice. (dovepress.com)
In the present study, the relation between deoxycytidine kinase (dCK) activity and the radiosensitising effect of gemcitabine was investigated in eight different human tumour cell lines. (biomedcentral.com)
A clear concentration-dependent radiosensitising effect of dFdC was observed in all cell lines. (biomedcentral.com)
The degree of radiosensitisation was also cell line dependent and seemed to correlate with the sensitivity of the cell line to the cytotoxic effect of dFdC. (biomedcentral.com)
Other partners of the intracellular metabolism of gemcitabine in relation to the cell cycle effects and DNA repair could be more responsible for the radiosensitising effect than dCK activity. (biomedcentral.com)
However, the latter effect, in turn, resulted in increased M2 subunit mRNA transcription (a process blocked in G 0 /G 1 -phase cells, with full-length functional M2 transcripts being generated only during S phase) and, hence, in a return to normal levels of dATP and to a normal rate of cellular DNA synthesis. (tmu.edu.tw)
Based on antineoplastic effect of brivaracetam and lacosamide on glioma cells, we assume that patients with glioma could benefit by the treatment with these two molecules, in addition to standard therapeutic options. (biomedcentral.com)

endothelial cells within the lining of blood veins throughout disbursement, can be advantageous. (esb-itathematic.it)

Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
Therefore, in this study, the anticancer effects of terpinen-4-ol were evaluated on two NSCLC cell lines, namely, A549 and CL1-0 human lung adenocarcinoma cells. (hindawi.com)
Due to the increased drug and radiation tolerance, it is urgent to develop novel anticancer agent that triggers nonapoptotic cell death to compensate for apoptosis resistance. (oncotarget.com)
Once inside, the anticancer activities rely on their abilities to produce DNA breaks, thus leading to cell death. (lookformedical.com)
In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
There are primarily two types of lung cancer: Non-small cell lung cancer (NSCLC) and SCLC, and the former accounts for ~85% of all lung cancer cases ( 3 ). (spandidos-publications.com)
The most striking and characteristic metabolic alteration in cancer cells is anomalous glucose metabolism and cancer cells tend to utilize glycolysis to obtain energy even under aerobic conditions via a process called 'aerobic glycolysis' ( 9 ). (spandidos-publications.com)
It has been reported that lung cancer cells exhibit upregulated expression of all key glycolytic enzymes [hexokinase 2 (HK2), phosphofructokinase and pyruvate kinase (PK)] ( 13 ), suggesting that the essential enzymes of the aerobic glycolytic pathway have a critical role in the development of lung carcinoma. (spandidos-publications.com)
Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
Breast cancer cells were treated with varying concentrations (1 to 5 mg/ml) of Solanum nigrum extract for 24 and 48 h. (ijpsonline.com)
These findings indicated that Solanum nigrum extract induced cell death in MDA-MB-468 cells by two distinct mechanisms, apoptosis and autophagy, and these findings further suggest a possibility that this extract could be used to treat triple negative breast cancer. (ijpsonline.com)
Therefore, remedies based on the induction of programmed cell death attracted greater attention for cancer treatment [ 8 ]. (ijpsonline.com)
We used four MPM cell lines (MS589, H28, H2052, JMN), a normal mesothelial cell line (HM3), and a lung cancer cell line (H23) in survival studies utilizing bortezomib, cisplatin, and pemetrexed alone and in combination by administering concurrently or by varying the order of administration. (mesothelioma-line.com)
In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
Among lung cancers, nonsmall cell lung carcinomas (NSCLC) account for approximately 80% of lung cancer cases [ 1 ]. (hindawi.com)
In addition, there is no report on the antitumor effects of terpinen-4-ol against human nonsmall cell lung cancer cells. (hindawi.com)
The abnormal cell growth is one of the critical characteristics of cancer for invasion or spreading out to other parts of the body. (jcpjournal.org)
Cancer cells can grow unusually via limitless number of cell division and can avoid apoptosis. (jcpjournal.org)
And only recently, evidence has emerged of a Passiflora species having cytotoxic potential against different cancer cells. (naturalnews.com)
Based on these results, the researchers concluded that the cytotoxic activity of ELPA against cancer cells may be due to the synergistic action of flavonoids in it, which induces cell death by apoptosis and necrosis. (naturalnews.com)
Direct correlation between resistance to chemotherapy and activation of PKB was shown in several prostate cancer cell lines and in human tumorogenic tissues, and elevation of PKB levels in prostate tumor tissues, is clinically associated with HRPC (Yongde et al. (justia.com)
This research paper will focus on the underlying mechanisms of cancer development related to the consumption of processed foods, specifically by highlighting the production of reactive oxygen species (ROS), which are unstable molecules containing oxygen that can easily damage cells. (nhsjs.com)
Cancer is a complex disease characterized by uncontrolled cell division and has become a significant public health concern worldwide. (nhsjs.com)
In this study we compare the anti-proliferative effects of crude extract of Cannabis sativa and its main compound cannabidiol on different cervical cancer cell lines. (biomedcentral.com)
In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines. (biomedcentral.com)
In this study, we aimed to assess the neoplastic-like transformation potential of two forms of CNC derived from wood (powder and gel) in human pulmonary epithelial cells (BEAS-2B) in comparison to fibrous tremolite (TF), known to induce lung cancer. (cdc.gov)
In this study, we show that treatment with gypenoside L (Gyp-L), a saponin isolated from Gynostemma pentaphyllum , induced nonapoptotic, lysosome-associated cell death in human esophageal cancer cells. (oncotarget.com)
Taken together, this work suggested a novel therapeutic option by Gyp-L through the induction of an unconventional ROS-ER-Ca 2+ -mediated cell death in human esophageal cancer. (oncotarget.com)
Affibody molecules hold great promise as carriers of cytotoxic drugs for cancer therapy due to their typically high affinity, easy production, and inherent control of the drug molecules' loading and spatial arrangement. (mdpi.com)
Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)

To attempt to identify the relationship of the key regulator molecules in paclitaxel-induced apoptosis using two metastatic cell lines: the human prostate carcinoma LNCaP line and the cervical carcinoma HeLa cell line. (johnshopkins.edu)
Both LNCaP and HeLa cells were continuously exposed to clinically achievable concentrations of paclitaxel and observed for activation of programmed cell death as measured by cytotoxic dose-response curves, poly(adenosine diphosphate-ribose) polymerase cleavage, bcl-2 phosphorylation, and the activation of caspase-7 (interleukin-1 beta converting enzyme (ICE)-LAP3). (johnshopkins.edu)
Initially, we asked whether paclitaxel-induced bcl-2 phosphorylation is triggered by the spindle assembly checkpoint via an active cdc2 kinase-dependent pathway and whether phosphorylation of endogenous bcl-2 is the signal that activates cell death machinery. (johnshopkins.edu)
Paclitaxel-induced G 2 /M cell cycle arrest correlated with cdc2 kinase activity and bcl-2 phosphorylation. (johnshopkins.edu)

Information counsel that low concentrations of ethanolic extracts of S. planicaulis (completely different seasons) induced elevated DNA harm associated to oxidative stress and chemical composition. (esb-itathematic.it)

CONCLUSIONS: The cytodifferentiating, cytotoxic and cytostatic activities of Hemidesmus indicus offers a scientific basis for its use in traditional medicine. (unibo.it)

Besides, 12% of patients have second- or third-degree skin reactions that are sensitive to light, and about 18% of patients develop cutaneous squamous-cell carcinoma and keratoacanthoma or occurred at the same time [ 6 ]. (medsci.org)
SUMMARY BLURB Essential oil derived from the camphor tree acts by stimulating immune cell-dependent regression of skin tumors in a mouse model of cutaneous squamous cell carcinoma. (biorxiv.org)

In this report, we determine the biological and molecular response of cultured MPM cells to bortezomib alone and in combination with cisplatin or pemetrexed. (mesothelioma-line.com)

High micromolar concentrations of topotecan, which are unlikely to be achieved in plasma in human patients in vivo , were cytotoxic within 48 hr, induced DNA fragmentation, did not induce major cell cycle changes, failed to consistently alter BCL-2 or BAX protein levels but inhibited RNA synthesis and induced cleavable DNA/topoisomerase I complex formation. (aspetjournals.org)
In addition, cell apoptosis was measured by double staining, DNA fragmentation and annexin V-fluorescein isothiocyanate binding assays. (ijpsonline.com)
These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation and DNA fragmentation [ 14 ]. (ijpsonline.com)

Although CNC exposures are associated with pulmonary inflammation and damage as well as oxidative stress responses and genotoxicity in vivo, studies evaluating cell transformation or tumorigenic potential of CNC's were not previously conducted. (cdc.gov)

Here, we have characterized the time- and concentration-dependent toxicity of topotecan in four human malignant glioma cell lines, LN-18, LN-229, LN-308 and T98G. (aspetjournals.org)
Apoptosis increased in a concentration- and time-dependent manner in 3 of 4 MPM cell lines. (mesothelioma-line.com)
Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
These 2n cells additionally confirmed the nuclear dependent 90° flip, which in each circumstances was related to cells gaining cell form modifications, herein illustrated from regular fibroblastic cells altering to roundness cells, indistinguishable from todays' diagnostic most cancers cell morphology. (esb-itathematic.it)
8 , 9 Cell cycle program is regulated by cyclin/cyclin-dependent kinase (CDK) complexes. (jcpjournal.org)
Our results showed a dose dependent cytotoxic response to HPMA. (niom.no)
On the basis of these studies, we then investigated whether bcl-2 was phosphorylated in a cell cycle-dependent fashion. (johnshopkins.edu)
Analysis of synchronized HeLa cells demonstrated that endogenous bcl-2 is phosphorylated in a G 2 /M cell cycle- dependent manner without apoptosis. (johnshopkins.edu)
Since dFdCTP accumulation and incorporation into DNA are concentration dependent, the degree of radiosensitisation seems to be related to the extent of dFdCTP incorporated into DNA required to inhibit DNA repair. (biomedcentral.com)
In cultured keratinocytes, CWO stimulated calcium signaling, resulting in calcineurin-dependent activation of nuclear factor of activated T cells (NFAT). (biorxiv.org)
Brivaracetam and lacosamide showed a dose-dependent cytotoxic and anti-migratory effects. (biomedcentral.com)

The analysis of cell differentiation by nitroblue tetrazolium (NBT) reducing activity, adherence to the plastic substrate, alpha-napthyl acetate esterase activity and morphological analysis was performed through light microscopy (LM) and transmission electron microscopy (TEM). (unibo.it)

In the present study, antiviral activity of four types of bioflavonoid against dengue virus type -2 (DENV-2) in Vero cell was evaluated. (who.int)
The researchers believe that the cytotoxic activity of ELPA is linked to the presence of the 10 flavonoids they have identified. (naturalnews.com)
Such activity would result in increased cell death of tumor tissue where PKB is elevated, and in decreased resistance to chemotherapy agents. (justia.com)
Exposure of cells to a higher concentration of BC-11 corresponding to its ED75 at 72 h (250 µM) caused additional impairment of mitochondrial activity, the production of reactive oxygen species and promotion of apoptosis. (figshare.com)
The dCK activity of our cell lines varied considerably and differed up to three fold from 5 to 15 pmol/h/mg protein between the tested cell lines. (biomedcentral.com)
The decoction caused differentiation of HL-60 cells as shown by NBT reducing activity, adherence to the plastic substrate, alpha-naphtyl acetate esterase activity, and increasing expression of CD14 and CD15. (unibo.it)

The complex molecules are preferably peptide conjugates having improved cell-permeability, serum stability and kinase selectivity compared to known protein kinase inhibitors. (justia.com)
The present invention relates to cell permeable, stable conjugates comprising a cell-permeability enhancement moiety and a peptide or peptidomimetic, as selective inhibitors of protein kinases, to pharmaceutical compositions containing them, as well as to processes for the preparation and use of such complex molecules. (justia.com)
Protein kinases are involved in signal transduction pathways linking growth factors, hormones and other cell regulation molecules to cell growth, survival and metabolism under both normal and pathological conditions. (justia.com)
Both BLM and A5 are hydrophilic molecules that depend on transporters or endocytosis receptors to get inside of cells. (lookformedical.com)

The aim of this study was to investigate the cytodifferentiative, cytostatic and cytotoxic potential of a decoction of Hemidesmus indicus's roots (0.31-3 mg/mL) on a human promyelocytic leukemia cell line (HL-60). (unibo.it)

Cell death pattern was analyzed by fluorescence microscopy of cells stained with propidium iodide (PI) and Hoechst 33342. (niom.no)

Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)

Prolonged exposure for 72 hr to high nanomolar to low micromolar concentrations of topotecan augmented p21 protein levels and induced G2/M arrest but failed to consistently alter BCL-2 and BAX protein levels, did not induce significant DNA/topoisomerase I complex formation and did not inhibit RNA synthesis. (aspetjournals.org)
JIB extract induced cell cycle arrest at the G 0 /G 1 phase and decreased cyclin and cdk protein expressions. (medsci.org)
Mechanistic investigations revealed that through increasing the levels of intracellular reactive oxygen species (ROS), Gyp-L triggered protein ubiquitination and endoplasm reticulum (ER) stress response, leading to Ca 2+ release from ER inositol trisphosphate receptor (IP 3 R)-operated stores and finally cell death. (oncotarget.com)
Interestingly, there existed a reciprocal positive-regulatory loop between Ca 2+ release and ER stress in response to Gyp-L. In addition, protein synthesis was critical for Gyp-L-mediated ER stress and cell death. (oncotarget.com)

To understand the molecular mechanisms responsible for the dermal toxicity of chromium , a differential gene expression profile of human skin fibroblasts exposed to a cytotoxic concentration of hexavalent potassium dichromate [Cr(VI)] was studied. (cdc.gov)
Because of this self-regulating mechanism, hydroxyurea-induced host-cell toxicity was obviated under conditions where HIV DNA synthesis, a process sensitive to both dATP depletion and the chain-terminating properties of the other inhibitory component of the combination (ddATP derived from dideoxyinosine), was unable to recover. (tmu.edu.tw)

Max Planck Institute (MPI) cells, a non-transformed mouse line that has an AM phenotype and genotype, were treated with mass-, surface area- (s.a.), and particle number- (p.n.) equivalent concentrations of respirable asbestiform and non-asbestiform riebeckite/tremolite EMP for 24h. (cdc.gov)

Autophagy, the type II cell death, is a physiological mechanism involving degradation of intracellular damaged proteins and organelles into membrane vacuoles. (ijpsonline.com)
Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)

Flow cytometric detection of reactive oxygen species (ROS) generation caused by TA-289 suggests that the compound may induce cell death via ROS production. (vuw.ac.nz)

Yeast mitochondrial morphology was visualized by confocal light microscopy, where it was observed that cells treated with TA-289 displayed abnormal mitochondria phenotypes, further indicating that the compound is acting primarily at the mitochondrion. (vuw.ac.nz)

Similarly, modulating the intracellular glutathione level by pre-treating cells with either BSO or glutathione significantly influenced the potential of Cr(VI) to induce the expression of the HO-1 gene. (cdc.gov)
Трансплантація гемопоетичних стовбурових клітин Hematopoietic stem cell (HSC) transplantation is a rapidly evolving technique that offers a potential cure for hematologic cancers ( leukemias, lymphomas, myeloma) and other hematologic disorders. (msdmanuals.com)

Transfer of a mutant p53 gene enhanced topotecan sensitivity in wild-type p53 LN-229 but not mutant p53 LN-18 cells. (aspetjournals.org)

These data suggest that PA-MSHA and Gefitinib function additively to suppress the proliferative effects of NSCLC cells of differential EGFR status. (oncotarget.com)

PKB was originally discovered as a viral oncogene v-Akt in rat T-cell leukemia. (justia.com)
Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)

respiratory burst (the rapid release of Reactions of oxygen radicals with Mitochondrial oxidative phosphor- reactive oxygen species from cells) cel ular components can deplete an- ylation is a major source of oxy- that involves nicotinamide adenine tioxidants, can cause direct oxidative gen radicals of endogenous origin. (who.int)

Dogs with digit or foot pad malignant melanoma (n = 4), osteosarcoma (n = 2), hemangiosarcoma (n = 1), transitional cell carcinoma (n = 1), nasal adenocarcinoma (n = 1), B-cell lymphoma (n = 1), or undifferentiated sarcoma (n = 2) were treated with 2 or 5 mg/kg c4G12 every 2 weeks. (bvsalud.org)
extract (JIB extract) for melanoma cells. (medsci.org)
Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma. (medsci.org)

The A549 human lung adenocarcinoma and CL1-0 lung adenocarcinoma cell lines were cultured in Dulbecco′s modified eagle medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic antimycotic. (hindawi.com)
Recent experiments with human cell lines of RCC have revealed a low alpha-beta ratio, ranging from 2.6 to 6.92. (cancernetwork.com)
An aggressive HeLa, a metastatic ME-180 and a primary SiHa cell lines were purchased from ATCC (USA, MD). Camptothecin was supplied by Calbiochem® and cannabidiol was purchased from Sigma-Aldrich and used as a standard reference. (biomedcentral.com)

The inducing issue was demise alerts to proliferating regular human cells (major cell strains), which respon-ded by a particular kind of tetraploidization, chromosomes with 4-chromatids (diplochromosomes, earlier described in most cancers cells). (esb-itathematic.it)

The addition of tubulin ligands can affect microtubule stability and function, including mitosis, cell motion and intracellular organelle transport. (wikipedia.org)
Microtubules are the key components of the cytoskeleton of eukaryotic cells and have an important role in various cellular functions such as intracellular migration and transport, cell shape maintenance, polarity, cell signaling and mitosis. (wikipedia.org)

Histopathology revealed a densely cellular, expansive, poorly delimited neoplasm composed of malignant mesenchymal cells arranged in islands and cords, interspersed by numerous areas of irregularly mineralized osteoid matrix. (bvsalud.org)
In addition, ozone oxidizes the outer lipid layer of malignant cells and destroys them through cell lysis (break- down). (silvermedicine.org)
Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. (biorxiv.org)

Anatomy30

Organisms19

Diseases11

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes58

Disciplines and Occupations1

Information Science3

Health Care2

Elicited a dose-dependent cytotoxic1

Cytotoxicity16

Shown to be cytotoxic2

Flow cytometry7

Evaluate the cytotoxic2

Assay10

Highly cytotoxic2

Apoptosis and cell cycle arrest1

Proliferation21

Maximal inhibitory concentration5

High concentrations5

Vitro5

Arrest and apoptosis1

Compounds10

Inhibition7

Inhibitor4

Genes5

IC504

Colorectal3

Tumor cells6

Underlying mechanism1

Methods2

Antioxidant6

Progression2

H2O21

Inhibits2

Effect14

Endothelial cells1

Anticancer5

Cancer27

Paclitaxel4

Extracts1

Conclusions1

Cutaneous squamous-cell carc2

Bortezomib alone1

Fragmentation3

Vivo1

Dependent13

Morphological1

Activity6

Molecules4

Investigate1

Fluorescence microscopy1

Prostate1

Protein4

Toxicity2

Line1

Membrane4

Induce cell death1

Compound1

Potential2

Sensitivity1

NSCLC1

Leukemia2

Mitochondrial1

Melanoma3

Lines3

Human1

Intracellular2

Malignant3