Concentration sequence and bind. Medical search. Frequent questions

At a higher-than-usual temperature (~42 °C), the RBS secondary structure of heat shock proteins becomes undone thus allowing ribosomes to bind and initiate translation. (wikipedia.org)
TTA proteins dimerize, and this dimer is capable of specifically binding tetracycline (or doxycycline, which we call dox, and also, these dimers can bind tetracycline operator sequences (called tet-o-secquences). (stanford.edu)
Therefore, the various concentration of the insulin proteins regulates the cell activity in different context, for instance in excess of glucose or lack of Growth Hormone. (proteopedia.org)
IGF binding proteins ( IGFBPs ) weight 24 to 45 kDa. (proteopedia.org)
As such, they can discriminate between closely related proteins, have fewer side effects, and a consequent lower concentration can convey a lesser likelihood of receptor desensitization. (nih.gov)
The IL-33 cDNA sequences encode 270 and 266 amino acid polypeptides for human and mouse, respectively, corresponding to full-length proteins with calculated masses of 30 and 29.9 kDa [ 2 ]. (hindawi.com)
When the intracellular concentration of autoinducers is low, autoinducer receptors act as kinases , transferring phosphate to LuxO (LuxO~P). LuxO~P promotes the expression of proteins used for building biofilms. (asm.org)
Several other RNA-binding proteins, including TLS/FUS and PSF, co-purify with EWS. (embl.de)
PKC phosphorylation of these proteins also inhibits their binding to RNA in vitro. (embl.de)
These data suggest that PKC may regulate interactions of EWS and other RNA-binding proteins with their RNA targets and that IQ domains may provide a regulatory link between Ca2+ signal transduction pathways and RNA processing. (embl.de)
Because CaM can bind with high affinity to a relatively small alpha-helical region of many proteins, success in clearly defining the essential elements of CaM binding motifs seems feasible and should provide a means of identifying CaM binding proteins. (embl.de)
Although considerable sequence diversity is observed among the different binding regions, these three classes of recognition motifs exist for many of the known CaM binding proteins. (embl.de)
Guanine nucleotide exchange factors ("GEFs") stimulate the dissociation of the GTP hydrolytic product, GDP, from small GTP-binding proteins, to promote the binding of a new GTP molecule. (justia.com)
When GTP is bound, for instance, Ras proteins can interact with effectors and other molecules to affect cell proliferation, differentiation and apoptosis. (justia.com)
PDZ domain proteins are frequently associated with the plasma membrane, a compartment where high concentrations of phosphatidylinositol 4,5-bisphosphate (PIP2) are found. (embl.de)
Its substrate specificity suggests that Tsp may contain a substrate recognition domain, which selectively binds to the nonpolar C-termini of substrate proteins, separate from its catalytic site. (embl.de)
In this work, we show that substrate recognition of Tsp is mediated by a PDZ domain, a small protein module that promotes protein-protein interactions by binding to internal or C-terminal sequences of their partner proteins. (embl.de)
Quantitative comparisons of RNA-binding sites showed that NXF1 and SR proteins bind mRNA targets at adjacent sites, indicative of cobinding. (uni-frankfurt.de)
Although, the number of omic techniques is ever expanding, the most developed omics technologies are high throughput DNA sequencing, transcriptomics (focused on gene expression), epigenomics (focused on epigenetic regulation of gene expression), proteomics (focused on large sets of proteins, the proteome) and metabolomics (focused on large sets of metabolites, the metabolome). (who.int)
Researchers speculate that it may bind to other proteins on the surface of cells or help cells attach to one another. (medlineplus.gov)

In contrast to mouse FcRn, which binds to IgGs from multiple different species, human FcRn is surprisingly stringent in binding specificity. (nih.gov)
Here, we have used site-directed mutagenesis in combination with interaction (surface plasmon resonance) studies, with the goal of generating human FcRn variants that more closely resemble mouse FcRn in binding specificity. (nih.gov)
These studies define the molecular basis for the marked difference in binding specificity between human and rodent FcRn, and give insight into how human FcRn recognizes IgGs. (nih.gov)
Ultimately these factors define the specificity of the antibody, that is, the likelihood that the particular antibody is binding to a precise antigen epitope. (sigmaaldrich.com)
no apparent DNA sequence specificity was detected. (rcsb.org)
The researchers also confirmed the specificity of the sensor from its inability to bind other DNA from other bacteria such as Escherichia coli. (phys.org)
SRSF3 emerged as the most potent NXF1 adaptor, conferring sequence specificity to RNA binding by NXF1 in last exons. (uni-frankfurt.de)

Furthermore, sequence homology between mammalian and fungal genes in the GPI pathway show modest conservation raising the chances of finding fungal-selective molecules with good therapeutic index 8 . (nature.com)
These 'networks' were inferred to be dynamic and poorly-ordered, consisting of several DNA loops between distally bound ParB molecules. (elifesciences.org)
Interesting, because the molecule contains 2 consensus sequences for ATP-binding (Walker-sequences), so either binding occurs also to non-Walker sequences or (more likely) there is co-operation between molecules. (bio.net)
Amplification was almost always used to increase the number of DNA molecules to be sequenced. (technologynetworks.com)
Studies aimed at defining those elements necessary for the design of synthetic double strand DNA cleaving molecules with defined target sequences and binding site sizes are described. (caltech.edu)
The covalent modification of sequence specific DNA binding molecules with a chelated metal capable of redox chemistry generates bifunctional DNA cleaving molecules. (caltech.edu)
This article discusses the consequences of the facilitated regulations, the impact of performing studies at very low pharmacologic concentrations, and the differences in regulation and pharmacology between smaller (organic) molecules and larger (protein biologic) molecules. (snmjournals.org)
In doing so, this GEF-facilitated exchange of GDP for GTP is associated with structural changes in the GTP-binding protein which influence the degree to which the GTP-binding protein can interact with other molecules. (justia.com)

Two recently published human COMT cDNA sequences differed in the position of S-COMT amino acid 108, the residue being either Val-108 [Lundstrom et al. (lu.se)

Single Molecule, Real-Time (SMRT) Sequencing… I can remember first reading about this technology in the mid to late 2000s. (technologynetworks.com)
When both domains bind the same DNA molecule the two halves interact to form functional β-lactamase, causing a measurable color changing reaction with nitrocefin. (willamette.edu)
2) If so, on what specific location of the glutathione molecule does RAPTA-C bind? (willamette.edu)

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling. (abcam.com)
The affinity constant for antibody-antigen binding can span a wide range, extending from below 105/mol to above 1012/mol. (sigmaaldrich.com)
Repeated equilibrium dialyses with a constant antibody concentration, but varying ligand concentration are used to generate Scatchard plots, which give information about affinity valence and possible cross-reactivity. (sigmaaldrich.com)
The S1 subunit (PDB I.D-6VSB) of the spike protein being reported to acts as receptor binding domain (RBD), binding to ACE-2 receptor with higher affinity. (ijpsonline.com)
In most cases, interaction between a PDZ domain and its target is constitutive, with a binding affinity of 1 to 10 microns. (embl.de)

For SMRT Sequencing of Whole Genome assemblies, we like to have DNA that is 20 kb and larger in average size. (technologynetworks.com)

Contagious human coronavirus (SARS-CoV-2) binds to the receptors of human host cells by transmembrane spike glycoprotein that makes homotrimers protruding from viral surface region[ 9 , 10 ]. (ijpsonline.com)
Spike glycoprotein has two subunits, S1-responsible for its binding to host cell membrane receptors and S2-responsible for the fusion of viral and host cellular membrane[ 8 , 11 ]. (ijpsonline.com)
Once intracellular concentration increases, autoinducers bind to their receptors, triggering signaling cascades that alter transcription factor activity and therefore, gene expression. (asm.org)
Intracellular autoinducer concentration in turn increases, autoinducers bind their receptors and change receptor activity from kinase to phosphatase. (asm.org)

The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)

In other words, the enzyme is going through the sequence of product binding, chemical catalysis, and product release continually. (cliffsnotes.com)
Because if the substrate concentration gets high enough, the enzyme spends all its time carrying out catalysis and no time waiting to bind substrate. (cliffsnotes.com)

To facilitate prospective surveillance for such evolution, we map how convalescent serum antibodies are impacted by all mutations to the spike's receptor-binding domain (RBD), the main target of serum neutralizing activity. (biorxiv.org)
Binding by polyclonal serum antibodies is affected by mutations in three main epitopes in the RBD, but there is substantial variation in the impact of mutations both among individuals and within the same individual over time. (biorxiv.org)
Our complete maps of how mutations impact serum antibody binding identify three major epitopes in the RBD. (biorxiv.org)
This presentation will describe the synthesis of Ru(η6-p-cymene)Cl2(pta), or RAPTA-C, and its binding interactions with human serum albumin, the most abundant transporter protein in the body. (willamette.edu)
A known ferrous iron standard of 105 μmol/L (586 μg/dL) was incubated with serum at a pH of 7.9, which saturates the available binding sites on serum transferrin. (cdc.gov)
Designations of S, I, and R derived from the MIC study usually correlate with achievable serum, plasma, or urine concentrations of free drug. (msdmanuals.com)

An illustration shows the base recognition step in avidity sequencing. (acs.org)
The ability of restriction endonucleases to cleave double helical DNA on opposite strands at or near 4-6 base pair recognition sequences makes possible DNA sequencing, gene isolation and recombinant DNA technology. (caltech.edu)
Sequence motifs for calmodulin recognition. (embl.de)

This is especially useful when multiple start codons are situated around the potential start site of the protein coding sequence. (wikipedia.org)

The clone VHH-28 resulted in the lowest 50% inhibitory concentration of 0.31 ng/mL in the phage enzyme-linked immunosorbent assay (ELISA) and was selected to develop the VHH phage-based real-time immuno-PCR (RT-IPCR). (cdc.gov)
Use at an assay dependent concentration. (abcam.com)
Typically, PCR is used to amplify a target sequence followed by a second assay to determine if the sequence contains a mutation associated with resistance. (cdc.gov)
The term microdosing, which was introduced through the work of such pioneers as Garner and Lappin ( 1 - 3 ), indicates the potential to assay the plasma pharmacokinetics of a very low administered mass in humans using a highly sensitive means of recording the concentration-time profile. (snmjournals.org)

There were two different mismatches in the probe target sites of the HA gene sequences of all isolates ( n = 23) with additional mismatches only at position 7 (template binding site) identified for all eight negative real-time RT-PCR isolates. (who.int)

In cells, they are presumably anchored at parS sites by sequence-specific interactions but must also contain many interactions with non-specific DNA (nsDNA), as well as self-association interactions that bridge ParB protomers to form DNA loops. (elifesciences.org)
Electrostatic interactions mediate the binding of the Tudor domain with DNA, which is consistent with NMR experiments performed at high salt concentration. (rcsb.org)
IGF-1 docks IGFBP with a series of polar and non-polar interactions all along its binding site. (proteopedia.org)
Compelling evidence for sequence-dependent intrusion of interactions under physiologically relevant solution conditions. (lu.se)

When enzymes bind multiple substrates, such as dihydrofolate reductase (shown right), enzyme kinetics can also show the sequence in which these substrates bind and the sequence in which products are released. (wikipedia.org)
in such cases, it is helpful to determine the enzyme structure with and without bound substrate analogues that do not undergo the enzymatic reaction. (wikipedia.org)
One frequent reason: Enzyme kinetic experiments performed with too few data points over a too narrow concentration range. (bio.net)
The monoclonal antibodies bind to the ACE2 receptor at a part of the protein that is distal to the active enzyme portion that converts angiotensin and does not modify its expression on the cell surface. (medscape.com)
Nonlinearity is typically introduced by processes such as binding and saturation of the target, as well as saturation of the enzyme and transport systems to which the drug is exposed. (snmjournals.org)
If an enzyme is added to a solution containing substrate, the substrate is converted to product, rapidly at first, and then more slowly, as the concentration of substrate decreases and the concentration of product increases. (cliffsnotes.com)
Just as any other chemical reaction can be favored by increasing the concentration of a reactant, the formation of an enzyme‐substrate complex can be favored by a higher concentration of substrate. (cliffsnotes.com)
Human soluble (S) and membrane-bound (MB) catechol O-methyltransferase (COMT, EC 2.1.1.6) enzymes have been expressed at sufficiently high levels in Escherichia coil and in baculovirus-infected insect cells to allow kinetic characterization of the enzyme forms. (lu.se)
The use of tight-binding inhibitors such as entacapone enabled the estimation of actual enzyme concentrations and, thereby, comparison of velocity parameters, substrate selectivity, and regioselectivity of the methylation of both enzyme forms. (lu.se)

The RBBP1 Tudor domain structure has a significant area of positively charged surface, which reveals a capability of the RBBP1 Tudor domain to bind nucleic acids. (rcsb.org)
Accordingly, the present invention envisions an isolated nucleic acid encoding the GEF-H1S polypeptide of SEQ ID NO:2, as well as an isolated nucleic acid comprising the sequence of SEQ ID NO:1. (justia.com)
Nucleic Acid-Based Identification Methods for Infectious Disease Nucleic acid-based methods detect organism-specific DNA or RNA sequences extracted from the microorganism. (msdmanuals.com)

The researchers aim to exploit the conservation of certain SARS-CoV-2 RNA motif sequences and structures. (news-medical.net)
Despite this inter- and intra-person heterogeneity, the mutations that most reduce antibody binding usually occur at just a few sites in the RBD's receptor binding motif. (biorxiv.org)
Short calmodulin-binding motif containing conserved Ile and Gln residues. (embl.de)
Calmodulin-binding motif. (embl.de)
A modified version of the IQ motif as a consensus for Ca2+-independent binding and two related motifs for Ca2+-dependent binding, termed 18-14 and 1-5-10 based on the position of conserved hydrophobic residues, are proposed. (embl.de)

Dyes used in these assays bind selectively to DNA making quantitation more sensitive than UV absorbance. (technologynetworks.com)

The DNA binding mode and key interaction residues were analyzed in detail based on a model structure of the Tudor domain-dsDNA complex, built by HADDOCK docking using the NMR data. (rcsb.org)
The DNA-binding residues are conserved in Tudor domains of the RBBP1 protein family, resulting in conservation of the DNA-binding function in the RBBP1 Tudor domains. (rcsb.org)

It is worth noting that this only holds up to a certain point - having too rich of a complementarity is known to paradoxically decrease the rate of translation as the ribosome then happens to be bound too tightly to proceed downstream. (wikipedia.org)
Because they bind so tightly, Element says avidites work at 100-fold lower concentrations than typical NGS detection bases. (acs.org)
For example, these triplets work together to bind tightly to RNA strands and unravel structures that block RNA copying. (elifesciences.org)

This step turns the DNA fragment into a closed construct that when opened by the DNA polymerase during sequencing looks like a big circle. (technologynetworks.com)

Variations of the 5'-AGGAGG-3' sequence have been found in Archaea as highly conserved 5′-GGTG-3′ regions, 5 basepairs upstream of the start site. (wikipedia.org)
Cross-reactivity is sometimes desirable when one wants general binding to a related group of antigens or when attempting cross-species labeling when the antigen epitope sequence is not highly conserved during evolution. (sigmaaldrich.com)
We have reported previously that Short Interspersed Degenerate Retroposons of the SIDER2 subfamily, largely located within 3'UTRs of Leishmania transcripts, promote rapid turnover of mRNAs through endonucleolytic cleavage within the highly conserved second tandem 79-nt hallmark sequence (79-nt SII). (uni-frankfurt.de)

We show here that while the central DNA binding domain is essential for anchoring at parS , this interaction is not required for DNA condensation. (elifesciences.org)

2017 to March 2018, there was circulation of both detect a single target in a very small concentration of DNA influenza A and B, with influenza A(H1N1)pdm09 again or RNA because it uses a fluorescent dye that binds to predominating in Viet Nam. (who.int)

Contaminated or damaged samples, along with inaccurate quantitation and sizing, can lead to downstream sequencing problems. (technologynetworks.com)

Dihydrofolate reductase from E. coli with its two substrates dihydrofolate (right) and NADPH (left), bound in the active site. (wikipedia.org)
The more substrate is present, the greater the initial velocity, because enzymes act to bind to their substrates. (cliffsnotes.com)

An avidity reagent with a fluorescent tag (green) includes multiple moieties of the same nucleotide, which can match multiple copies of the same template strand (inset box), increasing overall binding strength. (acs.org)
To address the mechanism, transcriptome-wide RNA-binding profiles of NXF1 and SRSF1-7 were determined in parallel by individual-nucleotide-resolution UV cross-linking and immunoprecipitation (iCLIP). (uni-frankfurt.de)
Sequence-dependent coordination of alkali ions to the nucleotide bases in the minor groove of AT-tract B-DNA has recently been inferred from X-ray crystallography, solution NMR and computer simulations. (lu.se)

Here we comprehensively map how all amino-acid mutations to the SARS-CoV-2 spike RBD affect binding by the antibodies in plasma collected from convalescent individuals ~1 to ~3 months post-symptom onset. (biorxiv.org)
In another embodiment, serine-67 is present in a peptide of less than 30 amino acids that comprises the sequence of SEQ ID NO. 4. (justia.com)
In another embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is less than 25 amino acids in length. (justia.com)
In a more preferred embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is 18 amino acids long. (justia.com)
It is 603 amino acids (aa) in length and contains a 25 aa signal sequence followed by three domains. (rndsystems.com)
Systematic deletion mutagenesis of Tsp localized the binding site to amino acids 206-307, a region that completely encompasses the putative PDZ domain (217-301). (embl.de)
The isolated PDZ domain (amino acids 206-334) is capable of folding into a well-behaved structure and binds to a nonpolar peptide with a dissociation constant (K(D)) of 1.9 microM, similar to that of the intact Tsp protein. (embl.de)
The Dutch type, the most common of all the types, is caused by the replacement of the amino acid glutamic acid with the amino acid glutamine at position 22 in the protein sequence (written as Glu22Gln or E22Q). (medlineplus.gov)

An example of enzymes that bind a single substrate and release multiple products are proteases , which cleave one protein substrate into two polypeptide products. (wikipedia.org)
however, the concentration of substrate is least in curve (a), greater in curve (b), and greatest in curve (c) . (cliffsnotes.com)
The slopes of the progress curves at early time, that is, the rate of product formation with time also increase with increasing substrate concentration. (cliffsnotes.com)

F-plasmid has a parS sequence cluster ( parS F , also called sopC ) composed of twelve repeats of a 16 bp consensus sequence, each separated by 27 base-pair spacer sequences ( Helsberg and Eichenlaub, 1986 ). (biorxiv.org)

This work has shown that single mutations to the spike's receptor-binding domain (RBD) or N-terminal domain (NTD) can appreciably reduce viral neutralization by polyclonal sera, sometimes by as much as 10-fold. (biorxiv.org)

Besides, it has a low limit of detection and can detect minute concentrations of up to 10 pM, thus facilitating quick and accurate diagnosis of infectious pathogens from extremely limited samples. (phys.org)

The phage particle, which displayed antibody fragments including single-chain fragment variable (scFv), variable domain of heavy-chain antibodies (VHH), and antigen-binding fragment (Fab) on the surface can be directly used in IPCR, supplying both the detection antibody and deoxyribonucleic acid (DNA) template. (cdc.gov)
Cross-reactivity refers to an antibody or population of antibodies binding to epitopes on other antigens. (sigmaaldrich.com)
Usually there are multiple bond formations observed, ensuring relatively tight binding between antibody and antigen. (sigmaaldrich.com)

Translation initiation happens following recruitment of the ribosome, at the start codon (underlined) found within the Kozak consensus sequence ACCAUGG. (wikipedia.org)
The Kozak consensus sequence was first identified by Marilyn Kozak in 1984 while she was in the Department of Biological Sciences at the University of Pittsburgh. (wikipedia.org)
Most ParABS systems have multiple copies of a ParB dimer binding consensus sequence that collectively constitute a parS site. (biorxiv.org)

Mostly, RBS refers to bacterial sequences, although internal ribosome entry sites (IRES) have been described in mRNAs of eukaryotic cells or viruses that infect eukaryotes. (wikipedia.org)
Bacterial mRNA are usually polycistronic and contain multiple ribosome binding sites. (wikipedia.org)
Photo-induced depletion of binding sites in DNA-PAINT microscopy. (nature.com)
Allosteric drugs bind to sites which are usually less conserved evolutionarily as compared to orthosteric sites. (nih.gov)
Mdr1 (and presumably other ABC-type ATPases) has no co-operation between ATP-binding sites? (bio.net)
Fe(II) (high resolution denaturing gel electrophoresis) provides information on the location, size, and orientations of distamycin binding sites on DNA. (caltech.edu)
Interestingly, SRSF3 and SRSF7 were shown to bind different sites in last exons and regulate 3' untranslated region length in an opposing manner. (uni-frankfurt.de)

Optimal spacing increases the rate of translation initiation once a ribosome has been bound. (wikipedia.org)
As V. cholerae reproduces, the extracellular concentration of autoinducers increases and eventually exceeds each bacterium's intracellular autoinducer concentration. (asm.org)

This is the approach taken by one study published in Nature Microbiology , which starts from a simple assumption: SARS-CoV-2 continues to modify its spike protein because of the evolutionary pressure of the antibodies produced by millions of infected people, but all these variants and subvariants, both present and future, enter cells by binding - not solely, but mostly - to the ACE2 receptor. (medscape.com)
The virus enters human cells via endocytosis by binding the ACE2 receptor and releasing its positive-sense RNA genome. (news-medical.net)

A ribosome binding site, or ribosomal binding site (RBS), is a sequence of nucleotides upstream of the start codon of an mRNA transcript that is responsible for the recruitment of a ribosome during the initiation of translation. (wikipedia.org)
This region of the mRNA has the consensus 5'-AGGAGG-3', also called the Shine-Dalgarno (SD) sequence. (wikipedia.org)
The level of complementarity of the mRNA SD sequence to the ribosomal ASD greatly affects the efficiency of translation initiation. (wikipedia.org)
Ribosome recruitment in eukaryotes happens when eukaryote initiation factors elF4F and poly(A)-binding protein (PABP) recognize the 5' capped mRNA and recruit the 43S ribosome complex at that location. (wikipedia.org)

Co-administration with elbasvir (EBR) and grazoprevir (GZR) due to the potential for significant decreases in plasma concentrations of EBR and GZR (see section 4.5). (who.int)

Structural analysis of the C-terminal domain reveals a dimer with a lysine-rich surface that binds DNA non-specifically and is essential for DNA condensation in vitro. (elifesciences.org)
DβH may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl - concentrations. (rndsystems.com)

Eukaryotic ribosomes are known to bind to transcripts in a mechanism unlike the one involving the 5' cap, at a sequence called the internal ribosome entry site. (wikipedia.org)
These nucleoprotein complexes act as a positional marker of the origin and earmark this region for segregation in a manner somewhat analogous to eukaryotic centromeres and their binding partners. (elifesciences.org)

NGS relies on sequencing by synthesis: the sequence of a template DNA strand is determined by synthesizing a complementary strand from fluorescently labeled bases. (acs.org)

It is both soluble (73 kDa) and membrane-bound (77 kDa) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine. (rndsystems.com)

PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)

others appear to bind internal (non-C-terminal) polypeptides. (embl.de)

Using three-letter 'triplet' building blocks, this new ribozyme can copy various folded RNA strands, including the active part of its own sequence. (elifesciences.org)
The structure of PDZ domains allows binding to a free carboxylate group at the end of a peptide through a carboxylate-binding loop between the beta-A and beta-B strands. (embl.de)

IGF-1 then acts as a ligand and can interact with Insulin Receptor protein and Insulin-like Growth Factor Binding Protein . (proteopedia.org)
Ligand-binding on α subunit induces activation of β subunit by autophosphorylation. (proteopedia.org)
Peptide binding of the ligand takes place in an elongated surface groove as an anti-parallel beta-strand interacts with the beta-B strand and the B helix. (embl.de)

Site-directed mutagenesis of a surface residue at the peptide binding site of the PDZ domain, valine 229, to Glu or Gln resulted in an increase in the K(M) value but had no effect on the k(cat) value. (embl.de)

Even subtle differences in protein sequence, in cellular metabolic states or in target tissues, can result in different outcomes. (nih.gov)

Molecular results obtained by DNA sequencing are listed in Table 2 [2]. (cdc.gov)

Mutation of either the dimerisation or the DNA binding interface eliminates ParB-GFP foci formation in vivo. (elifesciences.org)

Moreover, the free C-terminal domain can rapidly decondense ParB networks independently of its ability to bind DNA. (elifesciences.org)
Our work reveals a dual role for the C-terminal domain of ParB as both a DNA binding and bridging interface, and highlights the dynamic nature of ParB networks in Bacillus subtilis . (elifesciences.org)
ParB is an unusual DNA binding protein. (elifesciences.org)
have shown that binding of DNA by ParB is accompanied by condensation. (elifesciences.org)
ParABS partition systems, comprising the centromere-like DNA sequence parS, the parS -binding ParB-CTPase and the nucleoid-binding ParA-ATPase, ensure faithful segregation of bacterial chromosomes and low-copy-number plasmids. (biorxiv.org)
F-plasmid partition complexes containing ParB F and parS F move by generating and following a local concentration gradient of nucleoid-bound ParA F . However, the process through which ParB F activates ParA F -ATPase has not been defined. (biorxiv.org)
We studied CTP- and parS F -modulated ParA F -ParB F complex assembly, in which DNA-bound ParA F -ATP dimers are activated for ATP hydrolysis by interacting with two ParB F N-terminal domains. (biorxiv.org)

Recent research demonstrated that the homology between IGF-1R and the insulin receptor (IR) subunits allow them to bind and form a functionnal hybrid IR/IGF-1R receptor. (proteopedia.org)

Susceptibility testing occurs in vitro and may not account for many in vivo factors (eg, pharmacodynamics and pharmacokinetics, site-specific drug concentrations, host immune status, site-specific host defenses) that influence treatment success. (msdmanuals.com)
Semiquantitative methods determine the minimal concentration of a drug that inhibits growth of a particular organism in vitro. (msdmanuals.com)
Sequences may or may not be amplified in vitro. (msdmanuals.com)

The regulatory genes primarily the transcription factors that exert their activity by binding to certain cis DNA elements and consequently either up regulated or down regulate to target expression. (scielo.br)
It can also be customized for the detection of other pathogens apart from MRSA by modifying the target DNA binding sequence. (phys.org)

In this study, researchers showed that under certain conditions related to temperature, salt and protein concentrations, hnRNPA1's disordered tail prompts the protein to condense into liquid droplets through a process called liquid phase separation. (neurosciencenews.com)

The carrier-free buffer and high concentration allow for increased conjugation efficiency. (abcam.com)

The ion occupancies derived from the MRD data are substantially higher than previously reported, suggesting that groove-bound ions significantly influence the energetics and structural polymorphism of DNA in vivo. (lu.se)

In the absence of doxycycline, tTA dimers bind specifically to a concatamer of seven tetO sequences (7X tet-o) and activate oncogene transcription from the minimal TATA promoter. (stanford.edu)
Following binding to dox, tTA dimers undergo conformational changes that result in loss of sequence-specific DNA binding, and the oncogene is no longer activated. (stanford.edu)

All six IGFBPs share 50% homology with each other and have binding affinities at the same order of magnitude for IGF-1 and IGF-2 but have greater affinities then IGF1 for its receptor. (proteopedia.org)

This minimum inhibitory concentration (MIC) is reported as a numerical value that may then be translated to 1 of 4 groupings: S (sensitive), I (intermediate), R (resistant), or sometimes nonsusceptible. (msdmanuals.com)

However, for extrapolation to therapeutically relevant doses and plasma concentrations, there are specific aspects of the use of these low doses and low plasma concentrations that require special attention. (snmjournals.org)
A main obstacle is that the drug is intended to be used at pharmacologically relevant doses or plasma concentrations whereas the microdoses are at least 100-fold lower. (snmjournals.org)
We measured the plasma concentrations of a set of thirty-one biomarkers, comprising cytokines, chemokines and growth factors, in 987 plasma samples from a cohort of 572 pregnant women from five malaria-endemic tropical countries and related these concentrations to delivery outcomes (birth weight and hemoglobin levels) and malaria infection. (cdc.gov)
At recruitment, we found that P. vivax-infected pregnant women had higher plasma concentrations of proinflammatory (IL-6, IL-1beta, CCL4, CCL2, CXCL10) and TH1-related cytokines (mainly IL-12) than uninfected women. (cdc.gov)
Pro/arg-259 polymorphism affects tcn2 plasma concentration and may interfere in vitamin B(12) cellular availability and homocysteine metabolism. (lu.se)

For zidovudine triphosphate (AZT-TP), we predict that this selective advantage, as well as the minimal concentration required to select thymidine-associated mutations (TAMs) are highly cell-dependent. (plos.org)

Mutations are detected by lack of binding to wild-type probes and/or by binding to probes specific for commonly occurring mutations. (cdc.gov)

Moreover, SRSF5 is bound to pluripotency-specific transcripts such as Lin28a and Pou5f1/Oct4 in the cytoplasm. (uni-frankfurt.de)

Fe(II) (10 -6 M) sequence specifically single strand cleave pBR-322 plasmid DNA (10 -5 bp) in the presence of O 2 and dithiothreitol (DTT). (caltech.edu)

Anatomy22

Organisms17

Chemicals and Drugs99

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes80

Information Science3

Health Care1

Proteins20

Specificity7

Molecules8

CDNA sequences1

Molecule3

Affinity5

Whole Genome1

Receptors4

Peptide sequences2

Catalysis2

Serum6

Recognition3

Protein coding s1

Assay4

Gene1

Interactions4

Enzyme9

Nucleic3

Motif5

Selectively1

Residues2

Tightly3

Polymerase1

Highly conserved3

Interaction1

Fluorescent dye1

Downstream1

Substrates2

Nucleotide3

Amino8

Substrate3

ParS sequence1

Receptor bindin1

Detect1

Antibody3

Consensus sequence3

Sites7

Increases2

ACE22

MRNA4

Decreases1

Dimer2

Eukaryotic2

Synthesis1

Membrane-bound1

Precursor1

Polypeptides1

Strands2

Ligand3

Mutagenesis1

Differences1

Molecular1

Mutation1

ParB7

Subunits1

Vitro3

Target2

Researchers1

Conjugation1

Vivo1

Dimers2

Homology1

Inhibitory1

Plasma5

Dependent1

Probes1

Transcripts1

Specifically1