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RibonucleasesRibonuclease, PancreaticEndoribonucleasesRibonuclease HExoribonucleasesMolecular Sequence DataRibosome SubunitsGametogenesis, PlantSolanaceaePolyribonucleotidesAmino Acid SequenceRibonuclease T1Polyribonucleotide NucleotidyltransferaseRibonuclease IIIBase SequenceRNASubstrate SpecificityRNA Processing, Post-TranscriptionalRibonuclease PRNA, BacterialEscherichia coliSequence Homology, Amino AcidNucleic Acid ConformationPollenOligoribonucleotidesRNA StabilityPhylogenyRNA, TransferRibonucleoproteinsModels, MolecularKineticsSequence AlignmentCattleEvolution, MolecularChromatography, GelEnzyme StabilityCloning, MolecularRNA, Double-StrandedBinding SitesRNA PrecursorsAmino AcidsHydrolysisCarbohydratesChromatography, Ion ExchangePancreasEscherichia coli ProteinsProtein Structure, TertiaryPoly AFungiProtein DenaturationHot TemperatureMutagenesis, Site-DirectedMolecular WeightRNA, ViralPlant ProteinsSequence Homology, Nucleic AcidPlantsCrystallography, X-RayElectrophoresis, Polyacrylamide GelMutationSaccharomyces cerevisiaeRNA, MessengerDNAProtein ConformationHeLa CellsDNA PrimersBacterial ProteinsProtein BindingProteinsBacteriaTranscription, Genetic
Degraded duringDNaseReagentsInhibitorRibonucleasesAssayEukaryoticDNasesDegradationRecombinant humanSynthesisPolymeraseProteinBufferHydrolysisDegradesTotal RNAStrainCatalogReaction mixturePurificationAgaroseElectrophoresisMolecularReactionsEnzyme concentrationActivityHighPrepareFree waterHumanApplicationsProducts
Degraded during1
  • EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3). (neb.com)
DNase6
  • DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). (neb.com)
  • Product is RNase/DNase free. (thomassci.com)
  • In these cases, the QIAGEN RNase-Free DNase Set provides convenient on-column DNase treatment of RNA samples during QIAamp RNA procedures. (qiagen.com)
  • The following reagents were added to the DNase -treated reaction mixture: 30 μL RNase-free water to bring the volume to 50 μL, 5 μL 5 M Ammonium Acetate, and 3 volumes 100% ethanol. (nih.gov)
  • RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. (delos.info)
  • or add DNase/RNase to reduce the size of nucleic acid fragments. (sigmaaldrich.com)
Reagents1
Inhibitor23
  • RNase Inhibitor is ideal for RNA-sensitive applications such as RT-qPCR as even a small amount of RNase can be detrimental to the final experimental outcome. (meridianbioscience.com)
  • A 2-fold serial dilution of HeLa cell total RNA (1 μg - 0.075 μg) was reverse transcribed in the presence (lanes 1 - 5) and in the absence (lanes 7 - 11) of RNase Inhibitor, followed by the amplification of a 1 kb fragment of the angiotensin II receptor gene under standard PCR conditions. (meridianbioscience.com)
  • There was no detectable difference in PCR product in the presence or absence of RNase Inhibitor, indicating that the addition of RNase Inhibitor does not interfere with the function of reverse transcriptase during cDNA synthesis. (meridianbioscience.com)
  • The glycerol-free formulation ensures the compatibility of RNase Inhibitor (Glycerol Free) to lyophilized formats. (meridianbioscience.com)
  • Why do I need a RNase inhibitor? (meridianbioscience.com)
  • Do I always need to use an RNase inhibitor in my RT reaction? (meridianbioscience.com)
  • Can RNase inhibitor be used at higher temperatures? (meridianbioscience.com)
  • No, RNase inhibitor is a protein and will be denatured at higher temperatures. (meridianbioscience.com)
  • RNase Inhibitor is a recombinant human placental protein which inhibits ribonucleases (RNases) A, B and C. It does nit inhibit RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. (geneon.net)
  • One unit is the amount of enzyme required to inhibit by 50% the activity of 5 ng of RNase A at 250C (This inhibitor activity is determined by its ability to inhibit hydrolysis of cyclic 2', 3'-CMP by RNase A). (geneon.net)
  • biotechrabbit™ RNase Inhibitor is an acidic protein that is a potent inhibitor of a wide spectrum of ribonucleases. (biotechrabbit.com)
  • The RNase Inhibitor helps to prevent RNA degradation in applications like cDNA synthesis, RT-PCR, in vitro transcription/ translation reactions or RNA purification. (biotechrabbit.com)
  • The enzyme is purified from a recombinant E. coli strain carrying the RNase Inhibitor gene. (biotechrabbit.com)
  • RNase Inhibitor, 40 U/µl, in storage buffer containing 50% (v/v) glycerol. (biotechrabbit.com)
  • RNase Inhibitor can be used in all common reactions performed with RNA, it is active in all common buffers. (biotechrabbit.com)
  • The recommended final concentration for RNA protection is about 1 -2 units of RNase Inhibitor for every 1 µl of the reaction mixture. (biotechrabbit.com)
  • SecurRIN™ Advanced RNase Inhibitor is a premium tool for RNA protection from degradation during enzymatic reactions, storage or extraction. (biogen.cz)
  • SecurRIN™ Advanced RNase Inhibitor is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C binding them in a 1 to 1 ratio. (biogen.cz)
  • It is a recombinant protein derived from E. coli strain carrying human placenta RNase Inhibitor gene. (biogen.cz)
  • SecurRIN™ Advanced RNase Inhibitor does not inhibit RNAses H, 1, T1, T2 and S1 Nuclease. (biogen.cz)
  • 1 - 2 units of the RNase Inhibitor are typically enough for 1 microliter of RNA reaction. (biogen.cz)
  • Add SecurRIN™ Advanced RNase Inhibitor in to the cDNA synthesis reaction (~40 units for 20 µl reaction tube) as the first component. (biogen.cz)
  • In case high contamination with RNAses is suspected, use 2 µl of the RNase Inhibitor. (biogen.cz)
Ribonucleases1
  • Ribonucleases (RNases) are ubiquitous and can be introduced into experiments in many ways: for example co-purification during RNA isolation, carryover from bare hands and pipette tips. (meridianbioscience.com)
Assay3
  • In this work, we have tested the human canonical members of the RNase family using a spot-culture growth inhibition assay based mycobacteria-infected macrophage model for evaluating their anti-tubercular properties. (frontiersin.org)
  • Using the Local Lymph Node assay, AMT was identified as a sensitizer at concentrations of 15% and greater. (cdc.gov)
  • Cytokine modulation in draining lymph node cells was determined by RNase Protection Assay. (cdc.gov)
Eukaryotic1
  • Meridian RNase Inhibitors are highly efficient inhibitors of a broad spectrum of eukaryotic RNases and shows no inhibition of polymerase or reverse transcriptase activity, so can be used in cDNA synthesis or one-step PCR or quantitative PCR reactions. (meridianbioscience.com)
DNases1
  • The enzyme is free from DNAses and RNAses. (biogen.cz)
Degradation5
  • RNase E is a key regulatory enzyme in the pathway of mRNA degradation in Escherichia coli. (wikipedia.org)
  • It is able to auto-regulate the degradation of its own mRNA in response to changes in RNase E activity. (wikipedia.org)
  • The study encompassed dendrimers of generation one to four (G1-G4), modified to bear 6-48 amino end-groups, where the G2-G4 proved to be capable of siRNA complexation and protection against RNase-mediated degradation. (mdpi.com)
  • RNA is extremely sensitive to degradation by RNases present everywhere. (biogen.cz)
  • Each lot shows no detectable degradation of RNA when 5U of enzyme was incubated and 1x concentration of buffer was incubated with 500ng of RNA ladder for 1 hour at 37℃ as determined by agarose gel electrophoresis. (abasiabiolabs.com)
Recombinant human1
  • Out of the seven tested recombinant human RNases, we have identified two members, RNase3 and RNase6, which were highly effective against Mycobacterium aurum extra- and intracellularly and induced an autophagy process. (frontiersin.org)
Synthesis1
  • This rne 5′ UTR element acts as a sensor of cellular RNase E concentration enabling tight regulation of RNase E concentration and synthesis. (wikipedia.org)
Polymerase2
  • Poly(A) Polymerase can be inactivated by adding EDTA at a final concentration of 10 mM to the reaction. (neb.com)
  • Reaction composed of 1 µg of library plasmid, 1X transcription buffer, 0.5 mM of NTPs (GTP,ATP, CTP, and UTP), 40 U of SP6 RNA polymerase and 10 µl of RNase-free water. (nih.gov)
Protein3
  • Meridian RNase Inhibitors are from a recombinant protein that inhibits different RNases (A, B, C) by binding non-covalently in a 1:1 ratio. (meridianbioscience.com)
  • These factors can limit the reproducibility of peptide identification and quantification as well as the comprehensiveness, robustness and throughput of proteome analysis, particularly when analyzing samples of high complexity or wide dynamic range of protein concentrations as represented by tissues and body fluids 7 . (nature.com)
  • The optimal concentration of imidazole needed in the sample and buffer to obtain the best purity and yield differs from protein to protein. (sigmaaldrich.com)
Buffer4
  • An aliquot of the solution must be diluted into Buffer P1 to the appropriate concentration as described in the relevant plasmid purification kit handbook. (qiagen.com)
  • Dilute 10X buffer 10-fold with RNase free H 2 O. (openwetware.org)
  • To prevent the binding of host cell proteins with exposed histidines, it is essential to include imidazole at a low concentration in the sample and binding buffer ( Optimizing purification of histidine-tagged proteins ). (sigmaaldrich.com)
  • It is active in different buffer conditions within a broad pH range of 5.5 to 9.0, and within 0.5 - 1 mM concentration of DTT. (biogen.cz)
Hydrolysis2
  • One unit is defined as the amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A (hydrolysis of cyclic cytidine-monophosphoric). (biotechrabbit.com)
  • One unit is required to inhibit 5 ng of RNAse A by 50% (measuring the hydrolysis of cytidine 2′, 3′-cyclic monophosphate). (biogen.cz)
Degrades2
  • When the scientists pre-injected the mouse with the enzyme RNase, which degrades RNA, they found that the clot took around four times as long to develop. (chemistryworld.com)
  • Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids (see Figure 1 in Supporting Data). (pharmaceutical-sales.net)
Total RNA2
  • Total RNA binds to the QIAamp membrane and contaminants are washed away, leaving pure RNA to be eluted in 30-100 µl RNase-free water (provided with the kit) for direct use in any downstream application. (qiagen.com)
  • Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice. (delos.info)
Strain1
  • We have isolated a strain designated as Sphingopyxis strain MC4 that can tolerate and degrade high concentrations of α-, ß-, γ- and δ-HCH isomers. (bvsalud.org)
Catalog2
  • RNase A (17500 U), catalog number 19101, is 100mg/ml. (qiagen.com)
  • RNase A Solution (650 µl), catalog number 158922, and RNase A Solution (5 ml), catalog number 158924, are both 4mg/ml. (qiagen.com)
Reaction mixture2
  • To eliminate NotI-HF and possible RNase in reaction mixture, samples was subjected to Proteinase K treatment. (nih.gov)
  • SDS and Proteinase K were added to the reaction mixture to a final concentration of 0.5% and 100 µg/mL, respectively. (nih.gov)
Purification2
  • QIAGEN Ribonuclease A (RNase A) is endonuclease-free and quality-controlled for use in plasmid purification procedures for digestion of RNA. (qiagen.com)
  • This ready-to-use solution has the same specifications as the RNase supplied in all QIAGEN plasmid DNA purification kits. (qiagen.com)
Agarose1
  • RNase activity was not observed after agarose gel electrophoresis. (biotechrabbit.com)
Electrophoresis1
  • Pellet was dissolved in 50 μL RNase-free water and quality of RNA was assessed by agrose gel electrophoresis. (nih.gov)
Molecular1
  • In molecular biology, the RNase E 5′ UTR element is a cis-acting element located in the 5′ UTR of ribonuclease (RNase) E messenger RNA (mRNA). (wikipedia.org)
Reactions1
  • Yes, to be on the safe side, we highly recommend the use of RNase inhibitors in all RT reactions, since RNases are nearly everywhere, and it is very easy to contaminate samples during RNA preparation and reaction setup. (meridianbioscience.com)
Enzyme concentration1
  • Longer tails are produced by increasing the enzyme concentration in the reaction. (neb.com)
Activity2
  • Contribution of the enzymatic activity was discarded by site-directed mutagenesis at the RNase catalytic site. (frontiersin.org)
  • In addition to expression changes in tissues, more recent studies have indicated that miRNAs are detectable in serum, plasma, urine, and other body fluids in a highly stable form that is protected from endogenous RNase activity [ 4 ]. (hindawi.com)
High2
  • RESEARCH LETTERS for viral RNA with high-sensitivity assays, as we Triplex Real-Time RT-PCR are doing in Hubei Province, will be critical to en- sure blood safety. (cdc.gov)
  • Experimental infections of pigs confirmed that isolates obtained after introduction of ASF into Armenia and the Russian Federation cause acute disease and result in high mortality rates ( www.efsa.europa.eu/en/scdocs/scdoc/1556.htm ). (cdc.gov)
Prepare1
  • Prepare fresh 1:10,000 dilution in RNase free water of SYBR gold. (openwetware.org)
Free water2
Human1
  • Overall, our data anticipate a biological role for human antimicrobial RNases in host response to mycobacterial infections and set the basis for the design of novel anti-tubercular drugs. (frontiersin.org)
Applications1
  • With an association constant of 1014 M, they are useful in any applications where the presence of RNases is a potential problem. (meridianbioscience.com)
Products1
  • Even traces of RNase can nick the RNA, causing shortened cDNA products, low yields, and reduced RT-PCR sensitivity. (meridianbioscience.com)