• Soluble urokinase plasminogen activator receptor (suPAR) (NCBI Accession no. (wikipedia.org)
  • Urokinase plasminogen activator (uPA) and urokinase plasminogen activator receptor (uPAR) are the central molecules for uPA/uPAR/plasmin-dependent proteolysis, which is thought to play a significant role in the development of pregnancy, as well as its many complications during pregnancy. (ispub.com)
  • Elevated plasma concentrations of soluble urokinase plasminogen activator receptor (suPAR) provide early warning of progression to severe respiratory failure (SRF) or death, but access to suPAR testing may be limited. (hunimed.eu)
  • To assess the therapeutic efficacy of agents capable of stimulating the fibrinolytic system, such as tissue plasminogen activator (t-PA) and urokinase (UK) on endotoxin-induced disseminated intravascular coagulation (DIC) in the rabbit. (cun.es)
  • Tissue plasminogen activator (t-PA) and single chain urokinase - plasminogen activator (scu-PA) are relatively "fibrin-specific" thrombolytic drugs with short plasma half lives of 6-8 minutes. (northwestern.edu)
  • High anion gaps result from situations such as streptokinase, urokinase, anistreplase, tenecteplase, and rbinant tissue plasminogen activator (rtpa) enhance the likelihood of adherence to the inner corner of the large number of antiemetics, or other birth defects, principally has been established. (reflectionsbodysolutions.com)
  • uPAR is a membrane-bound receptor for uPA, also known as urokinase and Vitronectin. (wikipedia.org)
  • After adjustment for age, sex, smoking, education, exercise habits and alcohol consumption, levels of epidermal growth factor receptor (EGFR), and paraoxonase type 3 (PON3) remained positively associated with all three PFAS, while resistin (RETN) and urokinase plasminogen activator surface receptor (uPAR) showed inverse associations with all three PFAS. (lu.se)
  • Crystal structure of the urokinase receptor in a ligand-free form J Mol Biol. (virogates.com)
  • Structure of human urokinase plasminogen activator in complex with its receptor, Science. (virogates.com)
  • Goat anti Human Urokinase antibody detects an epitope within the C-terminal of urokinase, a potent ~50 kDa serine protease also known as urokinase plasminogen activator (uPA). (bio-rad-antibodies.com)
  • T2DN rats exhibited a significantly higher response to thrombin and urokinase. (figshare.com)
  • Moreover, the plasma concentration of thrombin in T2DN rats was significantly elevated compared to Wistar rats. (figshare.com)
  • In a concentration-dependent manner urokinase or thrombin abrogated the inhibitory effect of PAI-1. (regsj.dk)
  • Neither drug caused significant changes in the prothrombin time, activated partial thromboplastin time, thrombin time, hematocrit, platelet count, or fibrin degradation product concentration. (northwestern.edu)
  • We have demonstrated previously that oleoyl (18:1) LPA at concentrations present in ascites induces the secretion of urokinase plasminogen activator (uPA) from ovarian cancer cells, possibly linking LPA to cellular invasion. (spandidos-publications.com)
  • Estrella VC, Eder AM, Liu S, Pustilnik TB, Tabassam FH, Claret FX, Gallick GE, Mills GB and Wiener JR: Lysophosphatidic acid induction of urokinase plasminogen activator secretion requires activation of the p38MAPK pathway. (spandidos-publications.com)
  • uPAR promotes invasion by neoplastic or inflammatory cells by focusing proteolysis of urokinase to the cell surface. (virogates.com)
  • Direct intrathrombus injection of the thrombolytic agent protects the medication from deactivation by circulating inhibitors and achieves higher drug concentration at the site of thrombosis with a lower total dose than would be used for systemic intravenous thrombolytic therapy. (medscape.com)
  • In this Exchange Protocol we describe our method for determining the concentration of urokinase, an example protein of interest. (researchsquare.com)
  • Western blotting analysis of HEK293 lysate (35μg protein in RIPA buffer) using Goat anti Human urokinase antibody ( AHP1065 ) at 0.3μgml -1 . (bio-rad-antibodies.com)
  • The Severe COvid Prediction Estimate (SCOPE) score, derived from circulating concentrations of C-reactive protein, D- dimers, interleukin-6, and ferritin among patients not receiving non-invasive or invasive mechanical ventilation during the SAVE-MORE study, offers predictive accuracy for progression to SRF or death within 14 days comparable to that of a suPAR concentration of ≥6 ng/mL (area under receiver operator characteristic curve 0.81 for both). (hunimed.eu)
  • Type II: Only functional activity is decreased while protein concentration is normal (dysplasminogenemia). (medscape.com)
  • The suPAR concentration is positively correlated to the activation level of the immune system. (wikipedia.org)
  • The concentration of EGCG in the plasma was previously reported to be in the range of 0.1-0.3 µM, which is similar to levels in humans after drinking two or three cups of tea 11 . (nature.com)
  • Elevated concentrations of LPA are present in the ascites and plasma of ovarian cancer patients suggesting a role for LPA in the pathophysiology of ovarian cancer. (spandidos-publications.com)
  • This ELISA kit applies to the in vitro quantitative determination of Human suPAR concentrations in serum, plasma and other biological fluids. (elabscience.com)
  • At rest, heparin and Org 10172 did not influence the plasma concentrations of endogenous t-PA antigen and activity, urokinase-type PA (u-PA) antigen, plasmin activatable pro-urokinase (scu-PA), active urokinase (tcu-PA) and plasminogen activator inhibitor-1 (PAI-1) antigen. (chdr.nl)
  • Sulphated polysaccharides do not increase fibrinolytic activity of the plasma by changing the concentrations of the components of the fibrinolytic system. (chdr.nl)
  • With a single ingestion of the average therapeutic dose, the maximum concentration in plasma is noted after 3-4 hours, in synovial fluid after 4-5 hours. (bestpharmbuy.com)
  • Immunohistochemical staining of paraffin embedded human prostate with Goat anti Human urokinase antibody ( AHP1065 ) following heat induced antigen retrieval with citrate buffer pH 6.0. (bio-rad-antibodies.com)
  • Immunohistochemical staining of human placenta with Goat anti Human urokinase antibody ( AHP1065 ) following heat intuced antigen retrieval in citrate buffer pH6.0. (bio-rad-antibodies.com)
  • The fibrinolytic potential of human urine was first described in 1947, and the active molecule was named urokinase. (medscape.com)
  • One of the most thoroughly studied systems in relation to its prognostic relevance in patients with breast cancer, is the plasminogen activation system that comprises of, among others, the urokinase Plasminogen Activator (uPA) and its main inhibitor, the Plasminogen Activator Inhibitor-1 (PAI-1). (biomedcentral.com)
  • Abstract: BC-11 is an easily synthesized simple thiouronium-substituted phenylboronic acid, which has been shown to be cytotoxic on triple negative MDA-MB231 breast cancer cells by inducing a perturbation of cell cycle when administered at a concentration equal to its ED50 at 72 h (117 µM). (figshare.com)
  • Note: To avoid risk of other proteases add aprotinin (10 KIU/ml) in case buffer or urokinase solution is contaminated. (researchsquare.com)
  • Sucrose permeability was unaffected by high concentrations of aprotinin, indicating that transcytosis of aprotinin was unrelated to changes in the BBCEC monolayer integrity. (aspetjournals.org)
  • The inhibition appeared to be specific to endothelial cells because non-endothelial cells, including murine T241 fibrosarcoma tumour cells, murine fibroblast cells, and rat smooth-muscle cells, were insensitive to EGCG treatment at the concentrations used. (nature.com)
  • We demonstrated that angiostatin does indeed inhibit endothelial cell growth at the concentration produced [rom plasminogen. (subr.edu)
  • You can calculate the concentration of Human suPAR in the samples by comparing the OD of the samples to the standard curve. (elabscience.com)
  • SKOV3 cells were serum-starved for 16 h and then pretreated with different concentrations (100 and 1,000 nM) of H1 or H2. (icsv20.org)
  • SKOV3 cells pretreated with indicated concentration of H1 or H2 were incubated with 10 nM uPA and cell lysates were analyzed for SBC-115076 the (A) phosphorylated and total p42/44 as well as (B) MMP-9 and -actin by western blot analysis. (icsv20.org)
  • Treatment for 24 h of SKOV3 cells with H1 resulted in dose-dependent suppression of MMP-9 expression, starting at a concentration of 100 nM (Fig. 3B). (icsv20.org)
  • Exposure of cells to a higher concentration of BC-11 corresponding to its ED75 at 72 h (250 µM) caused additional impairment of mitochondrial activity, the production of reactive oxygen species and promotion of apoptosis. (figshare.com)
  • A thrombus at the tip of the catheter or a fibrin sheath around it may resist local thrombolysis if it is not reached by sufficient concentrations of the drug. (druglib.com)
  • A high concentration of hemoglobin, bilirubin, triglycerides and fibrin degradation products might affect PLG measurement. (medscape.com)
  • Platelet count, and fibrinogen, factor XII and antithrombin III concentrations, were assessed in each sample. (cun.es)
  • However, the effective concentration of EGCG seems to be too high for it to be physiologically relevant for tea drinkers 5 . (nature.com)
  • The inhibition of cell invasion occurs at high nano-molar concentrations. (icsv20.org)
  • The OD value is proportional to the concentration of Human suPAR. (elabscience.com)
  • Therefore, this indicates that elevated concentrations of suPAR can be ascribed to immune activation or immune response to a specific challenge. (virogates.com)
  • Urokinase activity was determined as amidolytic effect on the substrate pyro-Glu-Gly-Arg-p-nitroanilide-HCl. (researchsquare.com)
  • Urokinase activity vs absorbance correlation is linear between 5-40 Ploug units. (researchsquare.com)
  • Cell viability We performed MTS assays in SKOV3 and TOV21G with increasing concentrations of H1 or H2 (1,000 nM) for 24 h. (icsv20.org)
  • the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. (medscape.com)
  • Airborne concentrations found in various operations. (cdc.gov)
  • The oral bioavailability of glucosamine is 25% (the effect of "first passage" through the liver), the highest concentrations are found in the liver, kidneys and articular cartilage. (bestpharmbuy.com)
  • Significant increases in lysis were also seen with urokinase at ultrasound intensities of 2 W/cm2 and above. (jci.org)
  • Simultaneously prepare a solution, following the same procedure, by adding 25µl of Tris buffer instead of standard or sample urokinase. (researchsquare.com)
  • 1988. Standard test method for airborne asbestos concentration in workplace atmosphere- method D 4240-83. (cdc.gov)
  • Prepare urokinase standard by dilution of urokinase stock solution to 400 units/ml using water. (researchsquare.com)
  • Over a concentration range of 1 to 100 µg per disc, EGCG inhibited new blood-vessel growth in a dose-dependent manner ( Fig. 1g ), as measured by the formation of avascular zones. (nature.com)
  • Une étude a été menée dans des unités de soins intensifs en chirurgie pour adultes de l'hôpital universitaire de Zagazig, (Égypte) auprès de 25 patients atteints de septicémie, de 27 patients atteints d'une septicémie sévère et de 28 témoins. (who.int)
  • EGCG samples in triplicates and FGF-2 at a final concentration of 1 ng ml −1 were added to each well. (nature.com)