• The mechanism of cell death induced by RE was qualitatively measured using Acridine orange/Ethidium bromide staining and Hoechst staining. (biomedcentral.com)
  • Additionally, we examined TCCB cell viability using acridine orange/ethidium bromide (AO/EB) and MitoTracker staining. (oncotarget.com)
  • Apoptosis was checked by acridine orange/ethidium bromine staining assay and by execution of Western blotting analysis. (ijpsonline.com)
  • Acridine orange/ethidium bromine staining assay revealed that piperine induced apoptosis in MCF-7 cells which was further investigated by Western blotting. (ijpsonline.com)
  • Replication competence was found to correlate well with viability results from the acridine orange / ethidium bromide assay, but not with results from the trypan blue assay. (mit.edu)
  • Liver tissues were collected for assessment of oxidative stress markers (superoxide dismutase, catalase, reduced glutathione and malondialdehyde), protein concentration, DNA fragmentation, and also for haematoxylin and eosin staining, and acridine orange-ethidium bromide staining. (projectreserve.com)
  • LWA treatment could induce apoptosis in ascites cells evidenced by acridine orange/ethidium bromide, Hoechst, and Giemsa staining. (gp120inhibitor.com)
  • DNA fragmentation assay was done through Hoechst 33258 and acridine orange-ethidium bromide staining. (jcimjournal.com)
  • MTT assay and dual staining with acridine orange/ethidium bromide were done to assess the type of cell death induced by the enzyme in A549 cells. (greenpharmacy.info)
  • To evaluate the induction of apoptosis was carried-out by caspase-9 colorimetric assay and double staining acridine orange-ethidium bromide. (ijsr.net)
  • the identification of compounds was performed by gas chromatography-mass spectrometry (gc-ms). cells viability was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (mtt) and the mode of cell death was determined by acridine orange and ethidium bromide (ac/etbr) staining. (icbcongress.com)
  • These conditions included treatments at concentrations equivalent to 5885 μg enzymes concentrate dry matter/mL with and without the rat liver metabolic activation system (S9), and using a treat and plate assay. (europa.eu)
  • Concentrations were tested up to 8333 μg enzyme concentrate dry matter/mL), a recommended regulatory maximum concentration for the in vitro micronucleus assay. (europa.eu)
  • 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, and monodansylcadaverine staining and flow cytometry were performed. (hindawi.com)
  • Effects of Thuja occidentalis ethanolic extract on viability of A549 cell line (A) and L-132 cell line (B) Cells were exposed to Thuja occidentalis ethanolic extract for 24 h at different concentrations and the cell viability was determined by MTT assay. (jcimjournal.com)
  • Effects of Thuja occidentalis ethanolic extract on cellular proliferation tested by BrdU incorporation assay Cells were exposed to three different concentration of Thuja occidentalis ethanolic extract for 6, 12 and 24 h along with incorporation of BrdU. (jcimjournal.com)
  • Figure 1: Green versus red fluorescence cytograms and corresponding alpha t frequency histograms of Acridine Orange (AO) stained bull semen samples prepared and measured by the Sperm Chromatin Structure Assay (SCSA). (purdue.edu)
  • mtt assay revealed that almond hull extract inhibited the proliferation of osteosarcoma calls (saos-2) in a concentration dependent manner. (icbcongress.com)
  • Diagnosis is routinely made by conventional microscopy (Giemsa-stained thick blood smear) or fluorescence microscopy after concentration of blood in capillary tubes and staining with acridine orange (quantitative buffy coat [QBC] analysis) ( 3 ). (cdc.gov)
  • Fe 3 O 4 nanoparticles (NPs) can be conjugated with acridine orange to create a hybrid nanomaterial with unique properties, such as the magnetic characteristics of magnetite and the fluorescence of acridine orange, making them useful for a variety of applications, including cell imaging, drug delivery, and magnetic separation. (sciforum.net)
  • Within 60 min of exposure to the drug, two sub-populations of organisms could be distinguished in cultures by their different acridine orange-associated fluorescence emissions of 550 nm. (aisces.com)
  • Lysosomes were labeled with the fluorescent vital dye acridine orange and the rate and extent of their fusion with yeast-containing phagosomes was monitored by fluorescence microscopy. (rupress.org)
  • The rate of usion as assayed by fluorescence was not affected by the number of particles ingested, serum concentration, or prior uptake of digestible or nondigestible substances. (rupress.org)
  • ACT‑3 significantly decreased the cell viability in a concentration‑dependent manner and induced different morphological changes at high concentrations. (iasp-pain.org)
  • Cell apoptosis is tested by treating the lysosomal membrane with acridine orange. (wikipedia.org)
  • Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
  • The effect of the combination on cell survival, autophaghic markers expression along with acridine orange staining and cell apoptosis was determined. (esmo.org)
  • Acridine orange radiates a red fluorescent color at an increased concentration in a lysosome that is undamaged. (wikipedia.org)
  • Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. (researchgate.net)
  • Dexamethasone and OP, but not 17 beta-estradiol, caused significant nuclear condensation after 3 hr of culture (acridine orange staining) or 4 hr of culture (propidium iodide staining). (unboundmedicine.com)
  • The percentages of viable rat or mouse cells after 27 hr of culture were decreased significantly by 10(-12) M OP or greater concentrations. (unboundmedicine.com)
  • Protein concentration was significantly higher in Group II, Group III and Group V, with Group II having the highest. (projectreserve.com)
  • Different concentrations of Solanum nigrum extract showed cytotoxic effect on MDA-MB-468 cells. (ijpsonline.com)
  • Increased incidence of chromosomal aberration were observed at cytotoxic concentrations of methyl isothiocynate in tests with mammalian cells, precluding any conclusion on its in vitro clastogenic potential. (europa.eu)
  • Oleocanthal inhibits the growth of several breast cancer cell lines at low micromolar concentration in a dose-dependent manner. (wikipedia.org)
  • The half maximal inhibitory concentration (IC 50 ) dose (282 μg/mL) was determined, and two other doses for dose-dependence study, one below the IC 50 dose (IC35 =188 μg/mL) and one above the IC 50 dose (IC65=376 μg/mL) were selected. (jcimjournal.com)
  • Compared to the higher concentration, low dose of RSV (10 µg/ml) exhibited significant anti-survival effect accompanied with cell accumulation at G0/G1phase, higher MDA and VEGF secretion. (esmo.org)
  • The transvector jets directed the partially diluted vapour through heated delivery lines to the chamber inlets where chamber supply air was added to produce the targeted exposure concentrations. (europa.eu)
  • Although test substance usage was documented, it was not possible to determine nominal exposure concentrations. (europa.eu)
  • Analyzed exposure concentrations were determined at approximately 45-minute intervals using a gas chromatograph (GC) under the control of the WINH data acquisition software application. (europa.eu)
  • The IC 50 for NIH/3T3 was not detected even at highest concentration employed. (hindawi.com)
  • The highest concentration tested was 5000 µg/mL, tested as supplied (equivalent to 4585 ug enzyme concentrated dry matter/mL). (europa.eu)
  • Breast cancer cells were treated with varying concentrations (1 to 5 mg/ml) of Solanum nigrum extract for 24 and 48 h. (ijpsonline.com)
  • However, osmolality was not found to be a confounding factor as increased frequencies of MNBN cells were observed at concentrations below where any marked osmolality shifts were apparent. (europa.eu)
  • Anticancer effects of the HDAC inhibitor, 3β,6β‑dihydroxyurs‑12‑en‑27‑oic acid, in MCF‑7 breast cancer cells via the inhibition of Akt/mTOR pathways. (iasp-pain.org)
  • The susceptibility of individual bacterial cells to the antibiotic was assessed by differential staining using the metachromatic dye, acridine orange. (aisces.com)
  • Concentrations of insulin, a growth and survival factor used in CHO culture, were observed to drop rapidly in fed-batch cultures of CHO cells. (mit.edu)
  • There are some dyes that in low concentration can be used to stain living cells (e.g. iodine, acridine orange, methylene blue, Nile red) but most are toxic at high concentrations and the dyes can alter an organism or cells behaviour. (bioscint.com)
  • Lower concentration (80 μg/ml) showed arrest at S phase with 19% cells accumulated. (greenpharmacy.info)
  • Alh extract demonstrated a concentration dependent scavenging activity by quenching dpph radicals with sc50 value of 25 µg/ml. based on the colorimetric techniques, the total content of phenolic and flavonoid compounds at 0.625 mg/ml were 0.0645mg galic/g extract acid and 0.07254mg catechin/g extract, respectively. (icbcongress.com)
  • The combination of low RSV concentration (10 µg/ml) with autophagic inhibitor caused significant decrease in Beclin-1, LC3-B, BCl-2 and MCl-1 expressions, while higher doses of RSV (30 and 50 µg/ml) combination induced Beclin-1, BCl-2 and MCl-1 but reduced LC3-B expressions compared with the single treatment of RSV. (esmo.org)
  • At higher concentration (160 μg/ml), it showed an increased cell population at S phase with 30% with alterations in the other phase of cell cycle. (greenpharmacy.info)
  • Homocysteine concentrations are higher in postmenopausal women than in premenopausal women. (medscape.com)
  • Exponential phase cultures were exposed to the minimum bactericidal concentration of gentamicin and analysed at regular intervals over 90 min. (aisces.com)
  • Orange G is a monoazo dye that combines with proteins to produce Rayleigh scattering spectrum. (aisces.com)
  • Moreover, the concentration distributions of lactate and large signaling proteins in the culture chambers were measured using 4′,6-diamidino-2-phenylindole (DAPI) and fluorescein isothiocyanate-dextran (FITC-dextran). (oncotarget.com)
  • The above-described immune effect of all tested proteins was obtained at 20 g/100 g concentration with no further increments with 30- and 40 g/100 g protein in the diet. (immunehealthscience.com)
  • The highest concentrations of bacteria were observed during summer when also chlorophyll a concentrations were high, and therefore bacteria and phytoplankton were available in similar ratio at all seasons. (frontiersin.org)
  • The impact of hydrogen peroxide concentration on dye decolorization was examined on the Chitosan-HRP biocatalyst, since the HRP is susceptible to inhibition/inactivation by high H2O2. (ac.rs)
  • Because microscopic detection usually requires a microbe concentration of at least about 1 × 10 4-5 /mL, most body fluid specimens (eg, cerebrospinal fluid) are concentrated (eg, by centrifugation) before examination. (msdmanuals.com)
  • Previously, it was demonstrated that these aptamers can selectively deliver anticancer ligands," Dr. Cruz said, such as acridine orange derivatives, and showed improved selectivity towards cancer cell lines. (utexas.edu)
  • These conditions included treatments at concentrations up to 16667 μg enzymes concentrate dry matter/mL), in the absence and in the presence of a rat liver metabolic activation system (S-9) using a modified Treat and Plate methodology. (europa.eu)
  • Buy premium quality [ ORANGE G Indicator For microscopy ] and other lab chemicals from one of the most trusted brands. (aisces.com)
  • Orange G dye was incorporated in white rot fungal culture media to study the decolorization of azo dyes by the fungi. (aisces.com)
  • In the present study, various concentrations of docetaxel hidrate were incubated into an oral Burkitts lymphoma (Raji) cell line. (ijsr.net)
  • The use of a single vapor generator and a distribution system with excess vapor exhaust makes it inappropriate to calculate nominal concentrations. (europa.eu)
  • The principal factor responsible for the observed immune effect does not appear to be the availability or concentration of single essential amino acids but rather the composite effect of the specific amino acid distribution in the protein. (immunehealthscience.com)
  • Concanamycin A caused an increase of the cytosolic Ca 2+ -concentration whereas W-7 did not. (biomedcentral.com)