• Whereas the exoplasmic face of plasma membrane is preferentially labeled by both lectins, the endoplasmic reticulum and nuclear envelope are strongly and uniformly labeled by concanavalin A but not by wheat-germ agglutinin. (rupress.org)
  • The unique saccharide-binding properties of plant lectins, such as Concanavalin A (Con A) have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate. (polysciences.com)
  • Binding of the insecticidal lectin Concanavalin A in pea aphid, Acyrthosiphon pisum (Harris) and induced effects on the structure of midgut epithelial cells. (archives-ouvertes.fr)
  • Monodispersed maltoside-adsorbed gold nanoparticles aggregated with the specific lectin, concanavalin A (Con A). This phenomenon was used to detect the presence of Con A and to estimate concentrations of Con A in sample solutions. (ebscohost.com)
  • 1. The luminol-dependent chemiluminescence of rat thymocytes responding to concanavalin A can be resolved into glucose-dependent and glucose-independent portions. (biochemj.org)
  • 4. Preincubation of thymocytes for 18 h in serum-free medium enhances the subsequent chemiluminescent response to concanavalin A severalfold and evokes the response at a lower threshold concentration. (biochemj.org)
  • Concanavalin A (5 mg/L) was used to stimulate the cells, and then cells were cultured in a CO[sub]2 incubator at 37[degrees]C. (thefreedictionary.com)
  • Restoration of p53 functions protects cells from concanavalin A-induced apoptosis," Molecular Cancer Therapeutics , vol. 9, no. 2, pp. 471-479, 2010. (hindawi.com)
  • Electron-microscopic cytochemical studies on satellite cells of normal human skeletal muscle were carried out using the concanavalin Aperoxidase (Con A-HRP) coupling method. (springer.com)
  • The incorporation of [3H]thymidine by preincubated cells is similarly enhanced at low doses of concanavalin A, whereas the response to optimal doses is unaltered. (biochemj.org)
  • The combined application of thin-section and critical-point-drying "fracture-label" is used to determine the pattern of distribution and partition of wheat-germ agglutinin and concanavalin A binding sites on the membrane faces of freeze-fractured exocrine and endocrine rat pancreatic cells. (rupress.org)
  • Canine peripheral blood mononuclear cells were stimulated with Concanavalin A with or without polarizing cytokines. (umn.edu)
  • In this study , Concanavalin A and Manganese (II) Chloride were added to cell cultures containing Jurkat T-cells and an ELISA assay was conducted to determine IL-2 production . (viterbo.edu)
  • Their effect was dissimilar: glucagon decreased accumulation of intracellular cholesterol and the rate of 3H-thymidine incorporation into these cells, while concanavalin A increased the patterns studied. (msk.ru)
  • Vasil'ev A.V., Ren Li Khva, Orekhov A.N., Tertov V.V., Tutel'ian V.A., The effect of glucagon and concanavalin A on cholesterol accumulation in mouse peritoneal macrophages and human aorta intima cells, Voprosy meditsinskoi khimii, 1989, vol: 35(5), 90-93. (msk.ru)
  • Acosta-Serrano, A , Cole, RN & Englund, PT 2000, ' Killing of Trypanosoma brucei by Concanavalin A: Structural basis of resistance in glycosylation mutants ', Journal of Molecular Biology , vol. 304, no. 4, pp. 633-644. (elsevier.com)
  • A modified, quantitative assay for substances that inhibit the migration of macrophages and other leucocytes: the use of concanavalin A as a reference reagent. (semanticscholar.org)
  • Peroxidase labeled concanavalin A (Con A) permits the detection of some saccharide determinants. (biomedsearch.com)
  • An ultrasensitive electrochemiluminescent biosensor for the detection of concanavalin A based on poly(ethylenimine) reduced graphene oxide and hollow gold nanoparticles. (ebscohost.com)
  • Concanavalin A (Con A) kills procyclic (insect) forms of Trypanosoma brucei by binding to N-glycans on EP-procyclin, a major surface glycosyl phosphatidylinositol (GPI)-anchored protein which is rich in Glu-Pro repeats. (elsevier.com)
  • Zn2+ and Mn2+ are known to bind in site S1 and Ca2+ in site S2 of concanavalin A. Both sites must be occupied before monosaccharides will bind to the protein. (mysciencework.com)
  • Certain oligomannose type glycopeptides have previously been shown to be bivalent for binding to concanavalin A, and to give quantitative precipitation profiles with the protein that consist of single peaks which correspond to the binding stoichiometry of glycopeptide to protein monomer (1:2) (Bhattacharyya, L., Ceccarini, C., Lorenzoni, P., and Brewer, C.F. (1987) J. Biol. (elsevier.com)
  • 5. Catalase does not inhibit the enhanced incorporation of [3H]thymidine obtained in response to concanavalin A, but instead amplifies the response to low doses in the same manner as preincubation. (biochemj.org)
  • Restriction of patching of bound concanavalin a after incorporation of arachidonic acid into the plasma membrane of virally transformed fibroblasts. (jax.org)
  • Haptoglobin binding to Concanavalin A, immobilized to polystyrene microtiter plate, was estimated by anti-haptoglobin polyclonal and monoclonal antibodies conjugated with horse-radish peroxidase. (docphin.com)
  • Thus concanavalin A may activate, transiently, an NAD(P)H oxidase that utilizes reducing equivalents derived from the oxidation of exogenous glucose to give dismutation products of O2- (including H2O2) as its major products. (biochemj.org)
  • Concanavalin A (Con A) was fragmented by a proteolytic process and the m-Con A) derivative was isolated by elution with an ascending D-glucose gradient on a Sephadex G-200 column. (mysciencework.com)
  • This emission has been assigned to the breathing motion of the beta barrel consisting of 14 beta-strands in the concanavalin A molecule. (wikipedia.org)
  • Blockade of internalization by agents not interferring with phosphorylation, as hypertonic sucrose or concanavalin A, also blocked the resensitization (receptor functional recovering) process. (aspetjournals.org)
  • Development of concanavalin A-enzyme immunosorbent assay for glycated haptoglobin using polyclonal and monoclonal antibodies. (docphin.com)