PolymerasePrimersGenomicFragmentRecombinantSequenceBufferNucleic acidAmplificationQPCRDenaturationIsolatesGenusProteinNucleotideProteinsGeneSequencesReplicationStablePlasmidProtocolsPathogensNuclearComplementaryGenomeMethodsExtractionPrimerDetectionIntegrityFacilitatesSpecificMillerTeethSearchApplicationsDoublePatientTarget
Polymerase23
- Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
- miR-cDNA Synthesis Kit and miR-qPCR MasterMix comprises all the components necessary to perform Reverse Transcription and Real-Time PCR reactions: Reverse transcriptase, RNase inhibitor, miRNA specific stem-loop primer, antibody-mediated hot-start Taq DNA Polymerase, forward and reverse primers, dNTPs, MgCl2, SYBR Green dye, ROX reference dye, enhancer and stabilizer. (abtlabind.com)
- Φ29 DNA polymerase is an enzyme from the bacteriophage Φ29. (wikipedia.org)
- Each 5' end is covalently linked to a terminal protein, which is essential in the replication process by acting as a primer for the viral DNA polymerase. (wikipedia.org)
- it comprises a C-terminal polymerase domain and a spatially separated N-terminal domain with a 3'-5' exonuclease activity. (wikipedia.org)
- It is believed to be 1 or 2 orders of magnitude less error prone than Taq polymerase. (wikipedia.org)
- Highly efficient DNA synthesis by the phage Φ29 DNA polymerase. (wikipedia.org)
- The reaction mixture (total volume, 20 l) consisted of a master combination comprising Taq DNA polymerase, a deoxynucleoside triphosphate combination, and SYBR Green, 3 mM MgCl2, 5 pmol of primer 5-AGATCGTATCGCAGTCGAG-3, 5 pmol of primer 5-CCCAAGCTTGTAACCCTCTATGTGGAA-3), and 2 l of template cDNA (1/10 dilution). (massorrerjazzbar.com)
- 10 x A-attachment mix is a reagent comprising anti-KOD DNA polymerase antibody specific to KOD 3'→5' exonuclease activity (proof-reading activity)(1), as well as Taq DNA polymerase, which exhibits terminal transferase activity. (toyobo-global.com)
- PCR products from KOD-Plus- [Code No. KOD-201] and KOD FX [Code No. KFX-101] possess blunt ends due to 3'→5' exonuclease activity of the KOD DNA polymerase. (toyobo-global.com)
- The cloning efficiency of dA-attached PCR products is as great as PCR products from Taq DNA polymerase. (toyobo-global.com)
- The main components consist of the Taq DNA polymerase, the template, the primers, and loose nucleotides in the form of deoxynucleoside triphosphates (dNTPs). (norgenbiotek.com)
- 3 The polymerase can best be visualized as a piece of machinery whose job is to assemble new segments of DNA. (norgenbiotek.com)
- DNA is composed of two complementary strands of linked nucleotides in matched pairs of A/T and C/G. Thus if one strand is present, the polymerase can produce the opposite strand. (norgenbiotek.com)
- The new kits comprise a glycerol-free 4x qPCR mix with hot start Taq polymerase, dNTPs, MgCl₂ and a blend of excipients for reliable drying without activity loss. (clinlabint.com)
- Polymerase Chain Reaction (PCR) stands as a revolutionary technique in molecular biology, providing researchers with a powerful tool for amplifying specific DNA sequences with unparalleled precision and efficiency. (axis-shield-density-gradient-media.com)
- Denaturation separates the DNA strands, annealing allows primers to bind to the target sequence, and extension involves the synthesis of new DNA strands by a heat-stable DNA polymerase, often Taq polymerase. (axis-shield-density-gradient-media.com)
- The kit master mix consisted of 0.04 U/ml of Taq DNA polymerase, 10X PCR buffer, 3mM MgCl2 and 0.04 mM dNTPs (each). (ijbiotech.com)
- Both reactions had been in 25 mL mixtures including 5 μL from the NVP DPP 728 dihydrochloride reverse-transcription blend 10 Taq buffer with KCl 25 mM MgCl2 10 mM deoxyribonucleotide blend Taq DNA polymerase NVP DPP 728 dihydrochloride 10 pmol/L of every feeling and antisense primer and ddH2O (Takara Bio Otsu Japan). (ap26113.com)
- DNA Methods Limitation enzymes and T4 DNA ligase had been bought from New Britain Biolabs (NEB), and Taq polymerase was bought from Invitrogen. (buyresearchchemicalss.net)
- A heat stable DNA-DIRECTED DNA POLYMERASE from the bacteria Thermus aquaticus. (lookformedical.com)
- All organisms-bacteria, archaea, and eukaryotes-have a transcription initiation factor that contains a structural module that binds within the RNA polymerase (RNAP) active-center cleft and interacts with template-strand single-stranded DNA (ssDNA) in the immediate vicinity of the RNAP active center. (osti.gov)
- PCR (polymerase chain reaction) technique has achieved increased importance for post-mortem DNA analysis in forensic cases 8 because of the millions of copies amplified from one specific sequence of DNA. (bvsalud.org)
Primers3
- Primers give the start points and are short segments of DNA with the opposing sequence of base pairs on the template strand at the beginning of the segment of interest. (norgenbiotek.com)
- 1988). The 247 bp fragment, comprising a part of the IV exon and intron of the genomic DNA was amplified by using primers as suggested by Strazalkowska et al. (ijbiotech.com)
- Markers were analysed by the amplification of 50 ng of genomic DNA with the appropriate primers (GDB, Isogen Bioscience BV, The Netherlands). (bmj.com)
Genomic3
- Furthermore, molecular cloning serves as the foundation for constructing genomic libraries, which are comprehensive collections of cloned DNA fragments representing an organism's entire genome. (axis-shield-density-gradient-media.com)
- The sensitivity threshold for this PCR primer-based method was approximately 10 2 -10 3 colony forming units (CFU) per reaction, when H. pylori genomic DNA was used as a template. (biomedcentral.com)
- Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. (bvsalud.org)
Fragment4
- This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
- The term "cloning" refers to the replication of a specific DNA sequence or an entire organism, and in the molecular biology context, it primarily involves the generation of identical copies of a DNA fragment. (axis-shield-density-gradient-media.com)
- In molecular cloning, a target DNA fragment is inserted into a vector, often a plasmid or a viral genome, using specialized enzymes like restriction endonucleases and DNA ligase. (axis-shield-density-gradient-media.com)
- Amyloidogenic fragment"--An APP fragment comprising the .beta. (everypatent.com)
Recombinant6
- The resulting recombinant DNA is then introduced into a host organism, such as bacteria or yeast, where it can be replicated and propagated. (axis-shield-density-gradient-media.com)
- Recombinant DNA technology, an integral part of molecular cloning, has played a pivotal role in producing therapeutic proteins, developing vaccines, and advancing our understanding of gene function. (axis-shield-density-gradient-media.com)
- An immunogenic composition comprising 2 S. pneumoniae recombinant proteins wherein one of the proteins is selected from the Poly Histidine Triad family (PhtX) at a dose of between 1-100 µg and optionally comprising an adjuvant. (allindianpatents.com)
- In one embodiment, the present invention relates to a recombinant protein having a protein comprising at least a portion of the amino acid sequence set forth in SEQ ID NO:2. (justia.com)
- DNA sequencing and recombinant techniques soon permitted biosynthetic production of natural products, which for some organic synthesis proved intractable. (openwetware.org)
- Illness of macrophages with recombinant (mc26 and H37Ra had been cultured in Middlebrook 7H9 broth and 7H10 agar (Difco) comprising albumin dextrose complicated (5 g BSA, 2 g blood sugar and 0.85 g NaCl/L), 0.5% (v/v) glycerol and 0.05% Tween 80. (buyresearchchemicalss.net)
Sequence8
- Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
- The results of these analyses revealed that both the HPV6 E6 and E7 were highly conserved within the analyzed patient samples, and comprised only 3 types of variant sequence, respectively. (biomedcentral.com)
- analyzed the full DNA sequence of 48 H. pylori strains from GenBank ( http://www.ncbi.nlm.nih.gov/genbank/ ) and developed a PCR method that tests for H. pylori by specifically targeting the ureA gene [ 12 ]. (biomedcentral.com)
- The present invention also relates to a gene which hybridizes with the neurogenesis inducing gene encoding for a protein comprising at least a portion of the amino acid sequence set forth in SEQ ID NO:2. (justia.com)
- In another embodiment, the present invention relates to a gene comprising a DNA sequence having at least a portion of the nucleotide sequence set forth in SEQ ID NO: 1. (justia.com)
- In one embodiment, the nucleic acid comprises a nucleotide sequence having at least a portion of the sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 41, and SEQ ID NO: 43. (justia.com)
- Approximately 10 µg of total DNA from the algal culture (ITS sequence GenBank JF831923) was used to construct a library (sheared DNA fragments were of 500 bp length) for 100 bp × 100 bp paired-end sequencing using an Illumina GAIIx and standard Illumina protocols (Illumina, San Diego, California, USA). (bioone.org)
- 1. A purified protease which comprises the amino acid sequence SEQ ID NO:1, or a functional equivalent thereof produced by conservative amino acid alterations which result in a protease which exhibits the same biological, biochemical, chemical, physical and structural properties of the protease of SEQ ID NO:1. (everypatent.com)
Buffer2
- Taq buffer. (cdc.gov)
- Amplification involved 35 PCR cycles of one minute at 94°C, two minutes at 55°C, and three minutes at 72°C, which was carried out in a 15 μl reaction mixture containing 0.06 U Super taq in 1 × Super taq buffer (HT Biotechnology Ltd, England) and in the presence of [α 32 P]dCTP. (bmj.com)
Nucleic acid1
- citation needed] The isolated enzyme has no intrinsic helicase activity but may carry out an equivalent function by way of its strong binding to single stranded DNA, particularly in preference to double stranded nucleic acid. (wikipedia.org)
Amplification2
- It is being increasingly used in molecular biology for multiple displacement DNA amplification procedures, and has a number of features that make it particularly suitable for this application. (wikipedia.org)
- After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. (bvsalud.org)
QPCR2
- London-based PCR Biosystems has launched Air-Dryable Probe 1-Step Mix, a versatile all-in-one, air-dryable 4x RT-qPCR reagent mix for ultra-sensitive detection of RNA and DNA sequences. (clinlabint.com)
- QPCR allows precise quantification of DNA, while RT-PCR facilitates the study of gene expression by amplifying complementary DNA (cDNA) from RNA templates. (axis-shield-density-gradient-media.com)
Denaturation1
- At its core, PCR involves a series of temperature-mediated cycles, each comprising denaturation, annealing, and extension steps. (axis-shield-density-gradient-media.com)
Isolates2
- R2: 5-GGT GCC AGT TTC AGT AAC AC-3 (positions studies comprising a total of 77 and 260 Y. pestis isolates, 170402-170383) for initial PCR and glpD-F3: 5-CGC respectively, of the 4 biotypes ( 8,9 ). (cdc.gov)
- The 52 isolates comprised the following species: 31 Klebsiella pneumoniae , 13 Escherichia coli , seven Enterobacter cloacae and one Enterobacter aerogenes . (who.int)
Genus2
- A genus of the family POXVIRIDAE, subfamily CHORDOPOXVIRINAE, comprising bird poxviruses. (lookformedical.com)
- The genus Histoplasma capsulatum comprises three varieties: var. (bvsalud.org)
Protein1
- It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. (mdpi.com)
Nucleotide1
- We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
Proteins1
- Salinity stress generates reactive oxygen species (ROS), namely, H 2 O 2 , O −2 , and OH − that damage the DNA, RNA, and proteins [ 12 , 13 ]. (hindawi.com)
Gene1
- The detection of HPV DNA alone, in the absence of evidence for viral gene expression, is not unequivocal molecular evidence that HPV infection either causes or promotes malignant progression in a lesion. (biomedcentral.com)
Sequences1
- This process allows researchers to amplify and study specific genes or DNA sequences of interest. (axis-shield-density-gradient-media.com)
Replication1
- This method, conceptualized by Kary B. Mullis in 1983, has since become a cornerstone in various scientific disciplines, enabling the exponential replication of targeted DNA regions. (axis-shield-density-gradient-media.com)
Stable2
- In the case of COVID-19, the genetic material is comprised of Ribonucleic Acid (RNA) which is relatively fragile, as opposed to Deoxyribonucleic Acid (DNA), which is far more stable. (norgenbiotek.com)
- Kits can be used for both RNA and DNA detection for greater versatility, and dried assays are stable for 3 months at 37°C. (clinlabint.com)
Plasmid1
- Protocols for DNA manipulations, including plasmid DNA planning, restriction endonuclease digestive function, agarose gel electrophoresis, isolation and ligation of DNA fragments, and change had been performed as defined [20]. (buyresearchchemicalss.net)
Protocols1
- With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. (bvsalud.org)
Pathogens1
- Its impact on fields like forensics, medicine, and biotechnology is profound, enabling the study of genetic variations, identification of pathogens, and production of ample DNA for analysis. (axis-shield-density-gradient-media.com)
Nuclear3
- A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
- Based on a combination of mitochondrial and nuclear DNA, the following questions were addressed: 1) the phylogeography of the two O. h. robertsoni phylogroups, 2) regional and local population structure in space and time, and 3) patterns of local dispersal under different isolation-by-distance scenarios. (biomedcentral.com)
- Quarenta e nove isolados de Trichoderma obtidos no centro-oeste do Brasil foram avaliados quanto a sua atividade antagônica in vitro contra Sclerotinia sclerotiorum (agente causal do mofo branco) e identificados com base nas sequências ITS do DNA ribossômico nuclear. (scielo.br)
Complementary1
- This probe is yet another short segment of complementary DNA that adheres to the template and is destroyed when the copy is made. (norgenbiotek.com)
Genome1
- Φ29 is a bacteriophage of Bacillus subtilis with a sequenced, linear, 19,285 base pair DNA genome. (wikipedia.org)
Methods1
- Produces more DNA than PCR-based methods, by about an order of magnitude. (wikipedia.org)
Extraction1
- DNA was extracted, using the salting out extraction protocol (Miller et al. (ijbiotech.com)
Primer1
- Each reaction mixture consisted of 12.5 ml of the master mix, 1 ml of the DNA solution (50 to 100 ng/ml), 1 ml of each primer (5 pmol/µl) and some deionized water making up a final volume of 25 ml. (ijbiotech.com)
Detection4
- Human papillomavirus DNA detection in head and neck squamous cell carcinoma has been linked to improved patient prognosis. (biomedcentral.com)
- There was a significant positive association between the detection of HPV16 E6/E7 mRNA and p53 mRNA ( p = 0.032), but this was not replicated for HPV16 DNA. (biomedcentral.com)
- Simple HPV DNA detection alone did not demonstrate this association. (biomedcentral.com)
- In order to address this point, we have used a highly sensitive PCR detection method, together with a commercially available HPV genotyping kit (Linear Array HPV genotyping test, Roche Diagnostics Ltd., UK), to determine the presence of high- and low-risk HPV subtype L1 DNA in HNSCC tumours. (biomedcentral.com)
Integrity1
Facilitates1
- The enzyme facilitates the "debranching" of double stranded DNA. (wikipedia.org)
Specific1
- GABAergic neurons, the chief inhibitory neurons of the mammalian brain, are comprised of diverse neuronal subtypes distinguishable on the bases of location in given brain regions, microanatomical features, including dendritic arborizations and axonal projections, expression of specific macromolecules, and neurophysiological properties. (jneurosci.org)
Miller1
Teeth2
- Teeth have in their composition the most resistant tissue in the human body - dental enamel - which provides high resistance against adverse conditions that can degrade DNA in the whole dental structure 14 . (bvsalud.org)
- Due to this resilience against environmental changes (incineration, immersion, traumas, mutilation and decomposition), teeth represent an excellent source of DNA, when the other biological sources have been lost 20,29,30 . (bvsalud.org)
Search1
Applications1
- PCR's versatility extends across applications such as DNA cloning, genetic sequencing, and diagnostics. (axis-shield-density-gradient-media.com)
Double1
- Everyone knows the familiar double helix profile of DNA, which gives a good indication of its structure. (norgenbiotek.com)
Target1
- The result is an exponential increase in the quantity of the target DNA, making it amenable to subsequent analyses. (axis-shield-density-gradient-media.com)