• 17. The method according to claim 14, wherein the step of enriching comprises at least one method selected from: mitotic shake-off, countercurrent centrifugal elutriation (CCE), flow cytometry, and contacting the population of eukaryotic cells with a cell blocking composition. (freepatentsonline.com)
  • 2012. Productive performance and urinary excretion of mimosine metabolites by hairs heep grazing in a silvopastoral system with high densities of Leucaena leucocephala. (ucr.ac.cr)
  • In this study, we isolated a 1,520-bp complementary DNA ( cDNA ) for mimosinase from L. leucocephala and characterized the encoded enzyme for mimosine-degrading activity. (plantphysiol.org)
  • The purified recombinant enzyme was used in mimosine degradation assays, and the chromatogram of the major product was found to be identical to that of 3-hydroxy-4-pyridone ( 3H4P ), which was further verified by electrospray ionization-tandem mass spectrometry. (plantphysiol.org)
  • The isolation of the mimosinase cDNA and the biochemical characterization of the recombinant enzyme will be useful in developing transgenic L. leucocephala with reduced mimosine content in the future. (plantphysiol.org)
  • 2007. Influence of short-term Leucaena leucocephala feeding on milk yield and its composition, thyroid hormones, enzyme activity, and secretion of cattle. (ucr.ac.cr)
  • 2005. Milk and cheese fatty acid composition of sheep fed different mediterranean forages with particular reference to CLA cis-9, trans-11. (ucr.ac.cr)
  • 2009. Effect of species, cultivar and phonological stage of different forage legumes on herbage fatty acid composition. (ucr.ac.cr)
  • Considering its biochemical properties of inactivating various enzymes that require either bivalent metallic ions or PLP as cofactors, mimosine may have a role in plant defense, and based on its chemical composition, it may serve as a reservoir of carbon and nitrogen for survival and growth under nutrient-limiting conditions. (plantphysiol.org)