Substrate SpecificityPhosphorylationMolecular Sequence DataAmino Acid SequenceKineticsProtein KinasesProtein-Serine-Threonine KinasesSerineBinding SitesProtein BindingEnzyme ActivationCatalysisRecombinant ProteinsCell LineModels, MolecularProtein Structure, TertiarySignal TransductionCatalytic DomainMutationThreonineSequence Homology, Amino AcidProtein-Tyrosine KinasesEscherichia coliBase SequenceMutagenesis, Site-DirectedTyrosinePhosphoproteinsHydrolysisCalcium-Calmodulin-Dependent Protein KinasesRecombinant Fusion ProteinsPhosphoserineIsoenzymesPhosphopeptidesCasein KinasesCloning, MolecularHydrogen-Ion ConcentrationProtein Kinase CHeLa CellsCrystallography, X-RayProtein ConformationEnzyme InhibitorsAdenosine TriphosphatePhosphotransferasesElectrophoresis, Polyacrylamide GelTransfectionIntracellular Signaling Peptides and ProteinsStructure-Activity RelationshipCyclic AMP-Dependent Protein KinasesCOS CellsMolecular WeightPhosphothreoninePhosphotransferases (Alcohol Group Acceptor)Sequence AlignmentCells, CulturedModels, BiologicalCell Cycle ProteinsCyclin-Dependent KinasesAmino Acid MotifsCasein Kinase IICDC2 Protein KinaseMitogen-Activated Protein KinasesCattlePeptidesPeptide MappingBacterial ProteinsDNA-Binding ProteinsSaccharomyces cerevisiaeCarrier ProteinsAmino Acid Substitutionsrc-Family KinasesProto-Oncogene ProteinsProtein Processing, Post-TranslationalProteinsPrecipitin TestsDNA PrimersGlutathione TransferaseGlycogen Synthase Kinase 3G-Protein-Coupled Receptor Kinase 5Oxidation-ReductionSaccharomyces cerevisiae ProteinsBlotting, WesternPhosphotyrosineAmino AcidsCell MembraneMolecular StructureChromatography, High Pressure LiquidMembrane ProteinsPhosphoprotein PhosphatasesOligopeptidesTime FactorsNuclear ProteinsPeptide FragmentsPlasmidsEscherichia coli ProteinsCell NucleusMitogen-Activated Protein Kinase KinasesLiverImmunoblottingRibosomal Protein S6 Kinases, 90-kDaChromatography, Gel