Complexes phase separation. Medical search. Frequent questions

It forms through liquid-liquid phase separation (LLPS), leading to a dense phase in thermodynamic equilibrium with a dilute phase. (wikipedia.org)
A few years later in 2009 the role of liquid-liquid phase separation was further recognized to be involved in the formation of certain membraneless organelles by the biophysicists Clifford Brangwynne and Tony Hyman. (wikipedia.org)
Here, we found that the C-terminus of IRS-1 undergoes liquid-liquid phase separation (LLPS). (nature.com)
Pex13 undergoes liquid-liquid phase separation (LLPS) with Pex5-cargo. (tcdb.org)
For protein systems, we focus on block proteins that undergo liquid-liquid phase separation (LLPS) as a preassembly step for structural protein materials. (aalto.fi)
Complex coacervation is the phenomenon of liquid-liquid phase separation driven by electrostatic association of oppositely charged multivalent macromolecules in aqueous media, creating coacervate microdroplets enriched with charged moieties. (aps.org)
Liquid-liquid phase separation can be used for man-made encapsulation applications just as it has evolved for creating membrane-less intracellular compartments in biology. (usc.edu)
https://doi.org/10.1083/jcb.202205069 ) show Dvl2 undergoes liquid-liquid phase separation to stabilize β-catenin by pulling Axin into its biomolecular condensate at the plasma membrane. (rupress.org)
Recent studies found Axin ( 6 ) and the DC ( 7 ) undergo liquid-liquid phase separation (LLPS) and drive DC assembly. (rupress.org)

Coacervate (/koʊəˈsɜːrvət/ or /koʊˈæsərveɪt/) is an aqueous phase rich in macromolecules such as synthetic polymers, proteins or nucleic acids. (wikipedia.org)
In addition to maintaining specific organelle structures, phase separation enables hub proteins to assemble signalosomes which promote the speed of signaling outputs 11 , 12 . (nature.com)
Since these intrinsically disordered regions, which are conformationally dynamic and do not adopt stable secondary or tertiary structures, are often essential for mediating the phase transition of proteins 9 , it is therefore of interest to consider if the C-terminus of IRS-1 is involved in phase separation and to further delineate such implications upon insulin/IGF signaling. (nature.com)
A subunit of the enzymatic complex P-TEFb can induce compartmentalization of proteins into liquid-like droplets in cells. (natureasia.com)
Intrinsically disordered regions in Pex13 and Pex5 resemble those found in nuclear pore complex proteins. (tcdb.org)
Coacervate / condensate phase of polyelectrolytes and proteins. (aalto.fi)
In the absence of Wnt ligands, β-catenin is degraded via a destruction complex (DC) consisting of two core tumor suppressors that serve as scaffold proteins (APC and Axin) and two kinases (glycogen synthase kinase-3 β [GSK3β] and casein kinase 1 [CK1]) ( 4 ). (rupress.org)
CHOP researchers have shown that adenovirus proteins use a process called phase separation to coordinate production of viral progeny, which could have broad implications. (chop.edu)
I am doing my post doc in Marie Skepö's group, focused on phase separation mediated by intrinsically disordered proteins. (lu.se)

Coacervates can be characterized as complex or simple based on the driving force for the LLPS: associative or segregative. (wikipedia.org)
Associative LLPS is more complex to describe, as both solute polymers are present in the dilute and dense phase. (wikipedia.org)
Importantly, metabolic disease-derived G972R mutation results in a reduced ability of LLPS, potentially implicating the involvement of aberrant IRS-1 phase separation in various metabolic disorders. (nature.com)

Development of new or improved instruments, methods, and related software to elucidate 3D structures of macromolecules and macromolecular complexes. (nih.gov)

The emergent behavior of circle swimmers with explicit alignments was studied by generalizing the Vicsek model, where slow rotations enhance the polarization in macroflocks, while fast rotations induce secondary instabilities, leading to phase synchronized microflocks. (aip.org)
There are different energetic contributions governing their phase behavior in bulk and in thin films. (aps.org)
Here, we present our recent studies on the phase behavior of supramolecular nanocomposites in thin films. (aps.org)
Phase behavior and morphologies within both bulk and thin films were studied by TEM, AFM and X-ray scattering. (aps.org)
Betainium bis(trifluoromethylsulfonyl)imide forms one phase with water at high temperatures, whereas phase separation occurs below 55.5 degrees C (temperature switch behavior). (scienceopen.com)
The mixtures of the ionic liquid with water also show a pH-dependent phase behavior: two phases occur at low pH, whereas one phase is present under neutral or alkaline conditions. (scienceopen.com)

A reawakening of coacervate research was seen in the 2000s, starting with the recognition in 2004 by scientists at the University of California, Santa Barbara (UCSB) that some marine invertebrates (such as the sandcastle worm) exploit complex coacervation to produce water-resistant biological adhesives. (wikipedia.org)
For polyelectrolyte materials, we have worked on the role of water and ions on the thermal response of materials rising from complex coacervation (polyelectrolyte complexes and multilayers). (aalto.fi)

Even though SMN has been known to undergo phase separation for more than 25 years, the impact on its functions is not well understood and underappreciated. (sma-europe.eu)
For example, while the gas-liquid-like phase separation in equilibrium requires cohesive interactions, 1 active colloids can undergo motility-induced phase separation (MIPS) resulting from the combination of self-propulsion and steric repulsion. (aip.org)
Motility-induced phase separation (MIPS), the phenomenon in which purely repulsive active particles undergo a liquid-gas phase separation, is among the simplest and most widely studied examples of a nonequilibrium phase transition. (researchgate.net)

While most studies of biomolecular phase separation have focused on the condensed phase, relatively little is known about the dilute phase. (researchgate.net)

Note: low pH is crucial since at neutral pH DNA not RNA partitions into the aqueous phase. (openwetware.org)
After transfer of the metal ions to the aqueous phase, the ionic liquid can be recycled for reuse. (scienceopen.com)

A configuration where several phase domains persist and do not fuse, in these models, is described as microphase separation. (nature.com)
Models based on microphase separation seem particularly well-suited to explain the internal organization of euchromatin, but their applicability to this organization has not been tested. (nature.com)
Using block copolymers as matrices for organizing metal and semiconductor NPs through microphase separation leads to hierarchical NP assemblies, but control over NP location within the hosting domains is usually limited to one-dimensional distribution. (aps.org)
Such choice of polymeric components leads to a situation where the microphase separation of the block copolymer precedes the macrophase separation of the NPs from the copolymer. (aps.org)

Normal or reversed-phase liquid chromatography can be used for analysis. (springer.com)
For my master's thesis, I studied the retention mechanism in reversed-phase liquid chromatography with integral equation theories (replika Ornstein-Zernike theory and Wertheim's multi-density theory) as the basis of the theoretical models. (lu.se)

Grafting and stabilization of metal complexes for increasing catalytic activity have remained an enormous challenge in the catalytic arena. (researchsquare.com)
This programme of research focuses on a specific system of IDRs that regulate a range of vital cellular functions through a common factor, the protein kinase Aurora-A. Each IDR in this system achieves a distinct function by localizing Aurora-A to a different subcellular compartment, regulating its catalytic activity, and forming a multivalent complex that brings additional components of the relevant pathway into kinase proximity. (leeds.ac.uk)

It provides a bridge between the microscopic world of particles and the macroscopic world that we can observe, explaining phenomena like the behaviour of liquids and gases, phase transitions, and the thermodynamic properties of materials. (mpg.de)
In a new joint study, an international team from the Max Planck Institute for the Physics of Complex Systems, the University of California at Berkeley, the Lawrence Berkeley National Laboratory, the German Cluster of Excellence ML4Q and the Universities of Cologne, Bonn, and Sofia identified quantum computers as a natural platform to test the validity of Jarzynski's equality for many interacting quantum particles. (mpg.de)
One of the most intriguing phenomena in active matter has been the gas-liquid-like motility-induced phase separation (MIPS) observed in repulsive active particles. (aip.org)
In nanoparticulate phase-separating electrodes, phase separation inside the particles can be hindered during their charge/discharge cycles even when a thermodynamic driving force for phase separation exists. (mit.edu)
The ability of colloidal particles, under certain conditions, to self-organize suggests that colloidal particles could be used as precursors for advanced materials via the generation of complex microstructures. (purdue.edu)
We are currently developing theoretical and simulation methods to predict the magnitude and range of entropic forces between particles and between particles and surfaces of various shapes, to investigate phase separations in model colloidal dispersions, and to estimate coagulation/deposition rates and the kinetics of the phase transitions exhibited by entropically controlled dispersions. (purdue.edu)
In addition, we study the colloidal stability of dispersions of hydrates particles formed by complexes of hydrocarbons and water. (purdue.edu)

An enhanced surface adsorption is typically obtained from dilute mixtures of a polyelectrolyte and an oppositely charged surfactant under conditions when there is a bulk associative phase separation in the mixture. (lu.se)

however, these complexes have not been interrogated experimentally. (researchgate.net)

Colloid polymer mixtures exhibit vapor-liquid like and liquid-solid like phase transitions in bulk suspensions, and are well-suited model systems to explore confinement effects on these phase transitions. (springer.com)
These diverse materials may exhibit time-dependent structures under transient or out-of-equilibrium conditions resulting from for example self-assembly processes, phase transitions or in response to external fields, such as flow. (lu.se)

The structural variation of self-assembled lanthanide supramolecular complexes which can be induced by different factors such as concentration, anion and solvent, cationic radii, stoichiometric ratio and light. (rsc.org)

Our research focuses on macromolecular and colloidal assemblies but also complex externally driven assembly in soft matter. (aalto.fi)

The peroxisomal protein import machinery, which shares similarities with chloroplasts, is unique in transporting folded and large (up to 10 nm in diameter) protein complexes into peroxisomes. (tcdb.org)
Actin-related protein 2/3 (Arp2/3) complex activation by nucleation promoting factors (NPFs) such as WASP, plays an important role in many actin-mediated cellular processes. (elifesciences.org)

In this presentation, we will demonstrate our progress on stabilization of complex coacervate microdroplets composed of oppositely charged homopolyelectrolytes by addition of anionic comb polyelectrolytes. (aps.org)

The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. (springer.com)

The dispersed droplets of dense phase are also called coacervates, micro-coacervates or coacervate droplets. (wikipedia.org)
The conditions of the mixture in comparison to the two curves defines the phase separation mechanism: nucleation-growth of coacervate droplets (when the binodal region is crossed slowly) and spinodal decomposition. (wikipedia.org)

Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. (springer.com)
In normal phase liquid chromatography (NP-LC), the stationary phase is more polar than the mobile phase. (springer.com)
Chromatographic enantioseparation of amino acids using a new chiral stationary phase based on a macrocyclic glycopeptide antibiotic. (mpg.de)
SFC is mainly decided by the stationary phase nature. (lu.se)

There are several ways to model the separation mechanism: partition, adsorption, ion exchange, and size exclusion. (springer.com)

Electrostatic-based complex coacervates are the most common, and in that case the solutes are two polyelectrolytes of opposite charge. (wikipedia.org)
Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)

We describe a novel method to measure petroleum distillates in BAL fluid using gas chromatography-mass spectrometry (GC/MS). METHODS: n-Hexane, n-heptane, n-octane, methylcyclopentane, and cyclohexane were measured in BAL fluid specimens by headspace solid-phase microextraction/gas chromatography/mass spectrometry. (cdc.gov)

Often the phase-separation and enhanced deposition can be obtained by a simple dilution process. (lu.se)

For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)
For example, we have recently shown that liquid-to-vapor liquid nucleation is more appropriately described by an "activated instability", with the subsequent bubble growth phase occurring via a mechanism consistent with an unstable system. (purdue.edu)

The kinetics of phase separation is investigated by a Molecular Dynamics method, where hydrodynamic effects due to the solvent are included via the multiparticle collision dynamics method. (springer.com)
G. Sutmann, R.G. Winkler, G. Gompper, Simulating hydrodynamics of complex fluids: multi-particle collision dynamics coupled to molecular dynamics on massively parallel computers. (springer.com)
This instability could be the key to elucidating the complex charge/discharge dynamics in nanoparticulate phase-separating electrodes. (mit.edu)
While we show that our model reproduces the results of a porous-electrode model for the simple setup studied here, it is a powerful framework with the capability to predict the detailed dynamics in three-dimensional complex electrodes and provides further insights into the complex dynamics that result from the coupling of electrochemistry, thermodynamics, and transport kinetics. (mit.edu)
A drawback of SSFs is that difficult sampling and complex microbial dynamics pose an obstacle for analysis and predictions. (lu.se)

NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
Figure 2 shows how HILIC complements other areas of chromatography and extends the range of separation options. (springer.com)
For example, it is suitable for analyzing compounds in complex systems that always elute near the void in reserved-phase chromatography. (springer.com)
The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
Alkaline cupric oxide (CuO) oxidation is one of the mostly performance supercritical fluid chromatography (UHPSFC) adopted methods for analyzing lignin in various complex using columns with sub-2 m packing can further improve environmental matrices such as soils and sediments [9]. (lu.se)
UHPSFC , ultra- pacted by other parameters, such as the addition of the mod- high performance supercritical fluid chromatography ifier and the change of the mobile phase density [20]. (lu.se)

abstract = "In note separation of polyphonic music, how to separate the overlapping partials is an important and difficult problem. (ncku.edu.tw)

This phase separation might help P-TEFb to promote gene transcription. (natureasia.com)

Phase separation emerges as a general principle to regulate molecular functions under cellular stress conditions. (sma-europe.eu)

Effect of Salt Type and Alkyl Chain Length on the Binodal Curve of an Aqueous Two-Phase System Composed of Imidazolium Ionic Liquids. (acs.org)

6 The coarse graining of those microscopic linear models usually restores the detailed balance at the continuum level, and MIPS was mostly understood based on equilibrium-like phase separations. (aip.org)

The simulations on the kinetics of phase separation were carried out at HERMIT (HLRS). (springer.com)

Finally, the proposed method is applied to singing source separation. (ncu.edu.tw)
Recently, complex matrix factorization (CMF) is proposed by combining the phase information in source separation problem. (ncku.edu.tw)

Complex coacervates are a subset of aqueous two-phase systems (ATPS), which also include segregatively separated systems in which both phases are enriched in one type of polymer. (wikipedia.org)
Arthur Genthon of the Max Planck Institute for the Physics of Complex Systems, Albertas Dvirnas and Tobias Ambjörnsson (Lund University, Sweden) have now derived an exact equilibrium solution of an extended Poland-Scheraga model that describes DNA with a defect site that could, for instance, result from DNA basepair mismatching, cross-linking, or the chemical modifications from attaching fluorescent labels, such as fluorescent-quencher pairs, to DNA. (mpg.de)
Arthur Genthon of the Max Planck Institute for the Physics of Complex Systems, Takashi Nozoe (U. Tokyo, Japan), Luca Peliti (Santa Marinella Research Institute, Italy), and David Lacoste (Gulliver, Paris) have now developed a model-independent theoretical framework to address this issue. (mpg.de)
Scientists at the Max Planck Institute for the Physics of Complex Systems have now investigated a Rydberg atom perturbed by ground state atoms, exploiting hydrogen's infinite spectrum and high degeneracy to show that the Rydberg electron localizes in the same fashion as electrons in a disordered solid. (mpg.de)
Professor Corti's research focuses on understanding the thermophysical and kinetic properties of a variety of soft condensed-phase systems, including liquids and colloidal dispersions. (purdue.edu)
I'm from Minnesota (USA) and just graduated in 2021 from King's College London with my MSc in Complex Systems Modelling, where I also completed my master thesis with Forschungszentrum Juelich in computational systems biology. (lu.se)
1 Present address: Complex Systems Division, Lund University, Sweden. (lu.se)

In this letter, we present a very convenient and efficient technique of direct replication of biological structures via a two-step phase-separation micromolding process (PSμM). (acs.org)
Specifically, passive structures etched into the walls of the container can create entropic force fields of sufficient range and magnitude so that the motion and position of large colloids can be controlled, thereby generating a variety of two-dimensional fluidlike and solidlike phases on chosen templates. (purdue.edu)

It is exactly solvable for homogeneous DNA (with only one type of basepairs), and predicts a first-order phase transition. (mpg.de)
We observed a drop in the resistance around 20K in several superlattices ranging from (20nm/20nm) 4 to (5uc/5uc) 8 after exposure to the ultra-violet (UV) light which could be interpreted as a induced photo-induced phase transition. (mpg.de)

Orthologous to human MSL1 (MSL complex subunit 1). (nih.gov)

Separation in a magnetic field and washing removes materials not bound to the solid phase. (cdc.gov)
However, designing a catalytic system with complementary properties including high surface area, high loading, and easy separation offers a promising route for efficient utilization of magnetic material for various applications. (researchsquare.com)
Due to their magnetic properties, a magnetic field is applied easily to separate the sorbent from the solution, which makes the separation process simple, fast and highly effective. (researchsquare.com)

To estimate simultaneously the magnitude and the phase of STFT coefficients, this work paper developed a fully complex-valued deep neural network (FCDNN) that learns the nonlinear mapping from complex-valued STFT coefficients of a mixture to sources. (ncu.edu.tw)
Asphalt is a complex mixture of aliphatics, polycyclic aromatic hydrocarbons, substituted aromatic compounds, and other miscellaneous compounds. (cdc.gov)
Fluorescence was found to be problematic for the quantification of total PAHs from this complex mixture. (cdc.gov)

The team has unravelled signalling cues that control SMN's phase separation and proposes that separation and its regulation contribute to the function of the SMN complex, either in its established pathway or in novel pathways yet to be discovered. (sma-europe.eu)
However, the roles of phase separation in the regulation of many other signaling pathways await further exploration. (nature.com)

This suggests that no equilibrium-like phase separation theory can be constructed for chiral active colloids even with tiny active torque, in which no visible collective motion exists. (aip.org)

To overcome this, cells use a process called "phase separation" to bring certain components together in distinct domains and at the same time exclude those that are not needed. (sma-europe.eu)
Moreover, IRS-1 was found to form high-molecular-mass complexes in different types of cells 30 . (nature.com)
Induction and Monitoring of DNA Phase Separation in Living Cells by a Light-Switching Ruthenium Complex. (bvsalud.org)

The combination of new time- and spatial-resolved experimental methods combined with computational and theoretical approaches is required to determine the complex nonlinear and time-varying response to deformations which is often also non-homogeneous. (lu.se)

In complex ecosystems such as microbial communities, there is constant ecological and evolutionary feedback between the residing species and the environment occurring on concurrent timescales. (researchgate.net)

Nuclear and cytoplasmic phase separation enable novel functions of the SMN complex in RNP homeostasis. (sma-europe.eu)

It is well established that the SMN complex can "separate" from the cytoplasm and the nucleoplasm to form distinct "sections", particularly under cellular stress. (sma-europe.eu)
In a cellular model that mimics SMA, the team will evaluate if delay or defect in phase separation may contribute to SMN's malfunction in SMA. (sma-europe.eu)

With real science backing the design, our microgravity coffee cup will do more than lift espresso to astronauts' lips - it will also provide data on the passive movement of complex fluids as part of the Capillary Beverage investigation. (nasa.gov)

These results underscore the difficulty in assessing and quantifying the concentration of complex mixtures from occupational environments. (cdc.gov)

A desirable mobile phase would contain high organic content for better sensitivity and also show good on-column retention for polar ionic compounds. (springer.com)
This system is highly complex and introduces multiple unwanted aspects regarding high levels of noise, unknown physiological models of the cognitive aspects of listening and more. (lu.se)

To activate canonical signaling, Wnt ligands bind to a Fzd receptor/co-receptor complex to initiate signaling at the PM. This Wnt/receptor complex recruits Dvl to Fzd where Dvl serves as a binding partner for Axin. (rupress.org)

Anatomy13

Organisms5

Diseases1

Chemicals and Drugs78

Analytical, Diagnostic and Therapeutic Techniques and Equipment84

Psychiatry and Psychology5

Phenomena and Processes60

Disciplines and Occupations11

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture21

Information Science6

Health Care7

Liquid-liquid phase separation9

Proteins9

LLPS3

Macromolecules1

Behavior6

Coacervation2

Undergo3

Dilute phase1

Aqueous phase2

Microphase separation4

Reversed-phase liquid chromatography2

Catalytic activity2

Particles7

Polyelectrolyte1

Experimentally1

Transitions2

Supramolecular1

Macromolecular1

Protein2

Stabilization1

Characterization1

Coacervate2

Stationary5

Adsorption1

Electrostatic2

Mass spectrometry1

Deposition1

Mechanism2

Dynamics5

Chromatography6

Abstract1

Transcription1

Regulate1

Binodal curve1

MIPS1

Kinetics1

Source separation2

Systems7

Structures2

Transition2

Subunit1

Magnetic3

Mixture3

Regulation2

Suggests1

Cells3

Nonlinear1

Microbial1

Nuclear1

Cellular2

Fluids1

Mixtures1

High2

Receptor1