• Our goal is to understand the molecular mechanisms whereby the genomic RNA of influenza-like viruses is, on the one hand, the template for transcription and replication of the viral genome by its RNA-dependent RNA polymerase and, on the other hand, an Achilles' heel, whose recognition as non-self can trigger an innate immune response to counter the viral infection. (embl.org)
  • Background: Iwr1, a protein conserved throughout eukaryotes, was originally identified by its physical interaction with RNA polymerase (Pol) II. (johnshopkins.edu)
  • The RNPs were shown in white, with their coiled structures, and three-bulbed polymerase complex on the ends. (cdc.gov)
  • The proposed deep learning model will be set up to address the characterization of dynamic properties of protein-RNA interactions at the 3D level. (loria.fr)
  • The team benefits from experts in RNA-protein interactions and structural bioinformatics, as well as in computer science. (loria.fr)
  • Moreover, by leveraging the large amounts of data generated by MD simulations, experimental and computational techniques [3], deep learning can help uncover the underlying mechanisms that govern protein-RNA interactions. (loria.fr)
  • We established an efficient approach, crosslinking of segmentally isotope-labeled RNA and tandem mass spectrometry (CLIR-MS/MS), to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. (nih.gov)
  • Localized RNA backbone reversals stabilized by arginine-guanine interactions place the adenosines and guanines in reverse order in the duplex. (rcsb.org)
  • Deciphering protein–protein interactions. (crossref.org)
  • Many biological processes depend on protein-protein interactions. (jove.com)
  • Similar to ligand binding sites, interactions at a protein-protein interface may involve non-covalent bonds and hydrophobic forces. (jove.com)
  • Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. (jove.com)
  • Laboratory methods, such as affinity purification, mass spectrometry, and protein microarrays, can be used to identify new interactions. (jove.com)
  • Computer programs can predict PPIs based on similar interactions found in other proteins by comparing protein sequences and three-dimensional structures. (jove.com)
  • To understand these mechanisms, spatially resolved quantitative measurements of protein abundance, mobilities and interactions are needed, but current methods have limited capabilities to study dynamic parameters. (nature.com)
  • Here we describe a microscope based on light-sheet illumination 2 that allows massively parallel fluorescence correlation spectroscopy (FCS) 3 measurements and use it to visualize the diffusion and interactions of proteins in mammalian cells and in isolated fly tissue. (nature.com)
  • Imaging the mobility of heterochromatin protein HP1α (ref. 4 ) in cell nuclei we could provide high-resolution diffusion maps that reveal euchromatin areas with heterochromatin-like HP1α-chromatin interactions. (nature.com)
  • Slaughter, B.D., Schwartz, J.W. & Li, R. Mapping dynamic protein interactions in MAP kinase signaling using live-cell fluorescence fluctuation spectroscopy and imaging. (nature.com)
  • The main focus of our research is the development and optimization for High-Performing-Computing (HPC) of computational methodology for the structural and energetic characterization of protein interactions at molecular level. (bsc.es)
  • We aim to develop and optimize computational algorithms for characterizing and understanding protein-protein interactions, which remains one of the most important challenges in Structural Biology. (bsc.es)
  • More efficient identification of correct docking poses, especially in difficult cases such as weak interactions, multi-protein complexes or membrane proteins. (bsc.es)
  • Analysis of protein - protein interactions with other members indicated that the CCR4-NOT complex remained intact. (bvsalud.org)
  • A prerequisite for rapid adaptations is efficient RNA turnover, with endonuclease RNase Y playing a crucial role in mRNA stability as well as in maturation. (uni-marburg.de)
  • Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
  • In addition to the cis -elements in its 3′UTR and trans -acting factor Staufen, posterior localization of oskar mRNA requires deposition of exon junction complex (EJC) in its first exon through splicing ( Hachet and Ephrussi, 2004 ). (frontiersin.org)
  • The single strand of RNA acts as a template for RISC to recognize complementary messenger RNA (mRNA) transcript. (wikipedia.org)
  • Once found, one of the proteins in RISC, Argonaute, activates and cleaves the mRNA. (wikipedia.org)
  • The endonucleolytic cleavage of the mRNA complementary to the RISC's guide strand by Argonaute protein is the key to RNAi initiation. (wikipedia.org)
  • There are two main requirements for mRNA degradation to take place: a near-perfect complementary match between the guide strand and target mRNA sequence, and, a catalytically active Argonaute protein, called a 'slicer', to cleave the target mRNA. (wikipedia.org)
  • Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
  • c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)
  • d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
  • Myo4p, She2p, and She3p are hypothesized to form a heterotrimeric protein complex that directly transports ASH1 mRNA to daughter cells. (elsevierpure.com)
  • However, in the absence of She2p, Myo4p and She3p can be asymmetrically segregated to daughter cells by artificially tethering mRNA to She3p, implying that the transport and/or anchoring of the Myo4p/She3p complex is dependent on the presence of associated mRNA. (elsevierpure.com)
  • We are investigating how mRNA decay factors interact and/or interfere with the translation apparatus and how RNA modifications affect translation. (igbmc.fr)
  • Biodistribution of onasemnogene abeparvovec DNA, mRNA and SMN protein in human tissue. (harvard.edu)
  • RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)
  • Mutations affecting zipcodes, RBPs or motor-proteins required for neuronal mRNA localization were shown to lead to severe neurodegenerative diseases as ALS, FXTAS and FXS (7), underlining the need to understand the mechanisms that drive neuronal mRNA transport. (europa.eu)
  • Inhibiting the localisation of this mRNA leads to a significant reduction of CamKIIa protein at postsynaptic densities and a strong reduction of cognitive abilities in animal models (12). (europa.eu)
  • Also in this specific case, it is not understood how RBPs, potential adaptors and motor proteins, which are essential for the transport of CaMKIIa-mRNA are mechanistically contributing to its correct localisation. (europa.eu)
  • These proteins process pre-microRNAs into mature microRNAs (miRNAs) that target specific mRNA species for regulation. (hindawi.com)
  • Positive-sense RNA viruses possess a single-stranded RNA genome that can serve as messenger RNA (mRNA) that can be directly translated to produce an amino acid sequence. (msdmanuals.com)
  • ODE induced significant NOD2 mRNA and protein expression at 24 and 48 h, respectively, which was mediated via a NF-KB signaling pathway as opposed to a TNF-a autocrine/paracrine mechanism. (cdc.gov)
  • Biomolecules such as proteins and nucleic acids are at the heart of virtually all fundamental cellular processes. (loria.fr)
  • The recent improvements of such parameters have made them suitable for the analysis of nucleic acids and for characterizing conformational changes in RNA molecules. (loria.fr)
  • Is it possible to understand the molecular structure and function of proteins and nucleic acids in enough detail to make accurate predictions about structure and function? (stanford.edu)
  • These molecules include DNA, RNA and proteins although, in general, nuclear molecules exist as complexes of proteins and nucleic acids inside the cell. (frontiersin.org)
  • These studies are being extended to viral RNPs (the physiological RNA synthesis units) to understand the behaviour of the viral nucleoprotein during replication and transcription and to include host factors important for viral replication. (embl.org)
  • We later identified PIAS (protein inhibitor of activated STAT)xalpha as a DJ-1-binding protein, and found that DJ-1 restored androgen receptor (AR) transcription activity that was repressed by PIASxalpha. (nih.gov)
  • This interface is observed frequently in proteins that contain leucine zipper domains such as eukaryotic transcription factors. (jove.com)
  • Principal Findings: Here, we identify Iwr1 in a genetic screen designed to uncover proteins involved in Pol III transcription in S. cerevisiae. (johnshopkins.edu)
  • The main lines of our work are 1) reconstitution of the process with more than 50 pure proteins and mechanistic analysis, 2) structure determination of the 50 protein complex at atomic resolution, and 3) studies of chromatin remodelling, required for transcription of the DNA template in living cells. (stanford.edu)
  • Retroviruses use reverse transcription to create a double-stranded DNA copy (a provirus) of their RNA genome, which is inserted into the genome of their host cell. (msdmanuals.com)
  • Because RNA transcription does not involve the same error-checking mechanisms as DNA transcription, RNA viruses, particularly retroviruses, are particularly prone to mutation. (msdmanuals.com)
  • The complexes may induce transcription of messenger RNA, leading to the synthesis of new proteins. (bvsalud.org)
  • Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini. (rcsb.org)
  • Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
  • Some viruses have an outer envelope consisting of protein and lipid, surrounding a protein capsid complex with genomic RNA or DNA and sometimes enzymes needed for the first steps of viral replication. (msdmanuals.com)
  • Negative-sense RNA viruses possess a single-stranded negative-sense genome that first must synthesize a complementary positive-sense antigenome, which is then used to make genomic negative-sense RNA. (msdmanuals.com)
  • Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
  • We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)
  • MMDB and VAST+: tracking structural similarities between macromolecular complexes. (nih.gov)
  • Ameya Sinha , postdoc at the Helmholtz Institute Würzburg (HIRI), was awarded a Human Frontier Science Program (HFSP) Long-Term Fellowship for his project "Functional proteomics to dissect spatiotemporal dynamics of viral RNA bound macromolecular complexes" under the supervision of HIRI group leader Mathias Munschauer . (helmholtz-hiri.de)
  • Macromolecular complex annotations are imported from the Complex Portal . (yeastgenome.org)
  • The Jardetzky Laboratory is studying the structures and mechanisms of macromolecular complexes important in viral pathogenesis, allergic hypersensitivities and the regulation of cellular growth and differentiation, with an interest in uncovering novel conceptual approaches to intervening in disease processes. (stanford.edu)
  • RL11_THEMA ] This protein binds directly to 23S ribosomal RNA. (proteopedia.org)
  • While the C-terminal domain of L11 binds RNA tightly, the N-terminal domain makes only limited contacts with RNA and is proposed to function as a switch that reversibly associates with an adjacent region of RNA. (proteopedia.org)
  • To better understand how Lin28a promotes tissue repair, the researchers systematically looked at what specific RNAs it binds to. (eurekalert.org)
  • Complexes of RNA-binding proteins with ribonucleic acids (RNA). (bvsalud.org)
  • Researchers at Lund University Faculty of Medicine have determined a novel mechanism linking the metabolism of ribonucleic acids, RNA, to the development of leukemia in myelodysplastic syndrome patients, MDS. (lu.se)
  • Previously, we worked on aminoacyl-tRNA synthetases, which play an essential role in protein synthesis by charging specifically their cognate tRNA(s) with the correct amino acid and editing mischarged amino acids if necessary (Palencia et al . (embl.org)
  • Sci Rep. 2018) and nucleoside analogue, RNA synthesis inhibitors (Kouba et al . (embl.org)
  • Treatment wth olpasiran, a small interfering RNA product targeting lipoprotein (a) synthesis, led to a profound and sustained reduction in Lp(a) concentration when administered every 12 weeks in a new phase 2 study. (medscape.com)
  • Brain protein synthesis was measured using in vivo. (lu.se)
  • Brain protein synthesis was measured using in vivo labeling with [2H5] phenylalanine. (lu.se)
  • Cerebellar protein synthesis was increased by 19% at day 5 and 14% at day 9 after IGF-1 treatment. (lu.se)
  • My favorite way to view some of these is to open the sequence viewer, find and select the nucleic sequences, and color by residue to distinguish between the RNA and DNA. (scienceblogs.com)
  • In the nucleus, these complexes bind to specific DNA sequences and alter their expression. (bvsalud.org)
  • The cell counters RNA viruses with innate immune pattern-recognition receptors, such as the RNA helicase RIG-I, which recognise particular viral RNA structural motifs (e.g. 5′ triphosphate-dsRNA) as non-self, thus activating a signalling pathway leading to interferon production and establishment of the anti-viral state. (embl.org)
  • Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
  • The highly compact RNA has both familiar and novel structural motifs. (proteopedia.org)
  • To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)
  • Furthermore, a co-immunoprecipitation assay showed that the formation of a ternary complex between DJ-1, DJBP, and AR occurred in cells in which DJ-1 bound to the AR via DJBP. (nih.gov)
  • We report the crystal structure of a 58 nucleotide fragment of 23S ribosomal RNA bound to ribosomal protein L11. (proteopedia.org)
  • In view of its interaction with histones and non-nuclear histone proteins in the cell, extracellular DNA is likely to be bound with histones in the form of nucleosomes or chromatin. (frontiersin.org)
  • In the pathogenesis of SLE, immune complexes containing nuclear molecules play a key role in the inflammation characteristic of this disease, especially in the kidney ( Rekvig ). (frontiersin.org)
  • Not only is Dr. Goodsell a talented artist but he writes very clear descriptions of the ways molecules like proteins, RNA, and DNA work together and function inside a cell. (scienceblogs.com)
  • Among these proteins, annexin is known to inhibit phospholipase A2 and thus block the production of mediators and arachidonic acid metabolites such as the COX-2 enzyme, leukotrienes and prostaglandins, cytokines, interleukins, adhesion molecules and enzymes such as collagenase 24,25 . (bvsalud.org)
  • Among others, we have developed software for prediction of protein-protein complex structure by docking simulations, identification of binding sites on protein surfaces, or prediction of hot-spot residues from energy calculations. (bsc.es)
  • We have also made available our software through different web servers, such as pyDockWeb for protein-protein docking, pyDockSAXS for the structural prediction of protein complexes using Small-Angle X-ray Scattering (SAXS) data in collaboration with Pau Bernadó (CBS, Montpellier), or OPRA for the identification of RNA-binding residues in proteins. (bsc.es)
  • We made the RNA residues a little brighter since they're more chemically active. (scienceblogs.com)
  • The Cusack group uses X-ray crystallography and cryo electron-microscopy (cryoEM) to study the structural biology of protein-RNA complexes involved in RNA virus replication, innate immunity and cellular RNA metabolism. (embl.org)
  • A transcriptional arrest leads to a strong change in localization and dynamics of YmcA, YlbF and YaaT, supporting their involvement in global RNA degradation. (uni-marburg.de)
  • She2p is an RNA-binding protein that directly interacts with ASH1 cis-acting localization elements and associates with She3p. (elsevierpure.com)
  • Previously, we investigated the localization of several nuage-resident proteins in the nuage structures of spermatogenic cells by immunoelectron microscopy (IEM) [ 22 - 26 ]. (hindawi.com)
  • In addition, we determined that several nuage proteins localize to spermatogenic cell-specific structures, including mitochondria-associated granules (MAG), granulated bodies (GB), reticulated bodies (RB), and ribosome aggregates (RA) [ 22 - 26 ]. (hindawi.com)
  • His main interests are on the mechanisms of transmembrane signal instigation with a particular focus on G protein-coupled receptors and cytokine receptors. (stanford.edu)
  • Once the provirus is integrated into the host cell DNA, it is transcribed using typical cellular mechanisms to produce viral proteins and genetic material. (msdmanuals.com)
  • We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
  • The approach was tested on polypyrimidine tract binding protein 1 and U1 small nuclear RNP. (nih.gov)
  • After 4 weeks of conversion, we performed global analyses of RNA and protein levels by RNA-sequencing and mass spectrometry. (lu.se)
  • However, covalent disulfide bonds between cysteine amino acids on each protein surface may also play a role to keep them together. (jove.com)
  • The influenza virus's hemagglutinin (HA) surface proteins then bind to the sialic acid receptors on the surface of a human respiratory tract cell. (cdc.gov)
  • The structure of the influenza virus's HA surface proteins is designed to fit the sialic acid receptors of the human cell, like a key to a lock. (cdc.gov)
  • Specific steroid receptors in the cell cytoplasm bind to ligands for steroid hormone-receptor complexes that occsionally translocate to the cell nucleus. (bvsalud.org)
  • RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. (frontiersin.org)
  • The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
  • De Novo Variants in CNOT1, a Central Component of the CCR4-NOT Complex Involved in Gene Expression and RNA and Protein Stability, Cause Neurodevelopmental Delay. (bvsalud.org)
  • CNOT1 is a member of the CCR4-NOT complex, which is a master regulator, orchestrating gene expression , RNA deadenylation, and protein ubiquitination . (bvsalud.org)
  • RNA splicing is a major nexus of gene expression regulation, shaping cellular identity during development, frequently altered in human cancers. (lu.se)
  • RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
  • For instance, many enzymes, such as protein kinase A here, form a cleft that can recognize and bind polypeptide loops of their binding partners. (jove.com)
  • The adaptor protein Grb2 is able to enhance the activity of the cytoplasmic tyrosine kinase Btk through a novel mechanism, revealing a new role for Grb2 in B-cell signaling. (elifesciences.org)
  • PRKRA, a protein kinase, interferon-inducible double stranded RNA dependent activator protein kinase, also known as PACT, a protein activator of the interferon-induced protein kinase, is a product of PRKRA gene [ 1 , 2 ] and is a member of the dsRNA binding protein family [ 3 ]. (hindawi.com)
  • These mutants are defective for RNA-binding activity but retain the ability to interact with She3p, indicating that a functional She2p RNA-binding domain is not a prerequisite for association with She3p. (elsevierpure.com)
  • In other cases, different proteins or polypeptides come together to form a functional unit. (jove.com)
  • Thisimplies that BIN1 rather bundles actin than decorates single filaments.Third, we explored a strategy to purify an aggregation prone BAR protein.Aggregation is a property common in Peripheral Membrane Proteins. (kth.se)
  • The main goal of this PhD project is to elucidate the conformational dynamics of protein-RNA complexes and to develop a method for understanding their allosteric communications. (loria.fr)
  • The candidate will be supervised by Yasaman Karami (Inria researcher) with expertise in analyzing proteins conformational dynamics and drug design [1,2], and Malika SmailTabonne (associate professor, University of Lorraine) expert in machine learning. (loria.fr)
  • In this PhD the goal is to design and implement a method for characterizing the conformational dynamics of protein-RNA complexes using the set of trajectories generated from MD simulations in a systematic way. (loria.fr)
  • On the other hand, computational approaches have been developed to investigate the conformational dynamics in proteins [4,5,6]. (loria.fr)
  • To learn more about CRISPR and the Cascade proteins, iBiology has a great video interview with Jennifer Doudna , the woman who discovered this system. (scienceblogs.com)
  • Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
  • Another type of interface, known as helix-helix, or coiled-coil interaction, forms when helices of two proteins wrap around each other. (jove.com)
  • Transcriptional activation of the orphan nuclear receptor Nurr1 by ligands targeting its heterodimer partner retinoid X receptor alpha (RXRα) occurs through a nonclassical pharmacological mechanism involving ligand-binding domain protein-protein interaction inhibition. (elifesciences.org)
  • We studied the interaction between the lnc RNA and PC bybiophysical techniques. (kth.se)
  • Furthermore, the nuclear/cytoplasmic distribution for the N36S and R63K She2p mutants is not altered, indicating that nuclear/cytoplasmic trafficking of She2p is independent of RNA-binding activity. (elsevierpure.com)
  • Conclusions: Thus, Iwr1 plays an important role in preinitiation complex formation by all three nuclear RNA polymerases. (johnshopkins.edu)
  • Cell membranes need to change their shapes during many cellular processeslike protein trafficking, cytokinesis and membrane homeostasis. (kth.se)
  • The Puglisi group investigates the role of RNA in cellular processes and disease. (stanford.edu)
  • It was found that DJBP repressed a testosterone-dependent AR transactivation activity in monkey Cos1 cells by recruiting histone deacetylase (HDAC) complex, including HDAC1 and mSin3, and that DJ-1 partially restored its repressed activity by abrogating DJBP-HDAC complex. (nih.gov)
  • The above image shows the different features of an influenza virus, including the surface proteins hemagglutinin (HA) and neuraminidase (NA). (cdc.gov)
  • Following influenza infection or receipt of the influenza vaccine, the body's immune system develops antibodies that recognize and bind to "antigenic sites," which are regions found on an influenza virus' surface proteins. (cdc.gov)
  • The virus' hemagglutinin (HA) and neuraminidase (NA) surface proteins are displayed in semi-transparent blue sticking out of the surface of the virus. (cdc.gov)
  • Three of these RNP segments encode the virus' surface proteins, i.e., the HA, NA and M proteins. (cdc.gov)
  • Using single-molecule tracking, we show that all three Y-complex proteins exhibit three distinct mobilities, including movement through the cytosol and confined motion, predominantly at membrane-proximal sites but also within the cell center. (uni-marburg.de)
  • To learn about proteins and their activities, I like to go directly to the Molecule of the Month page, where I can find a list of articles organized by molecule type and name. (scienceblogs.com)
  • the genome of RNA viruses ranges from 3.5 kilobases (some retroviruses) to 27 kilobases (some reoviruses), and the genome of DNA viruses ranges from 5 kilobases (some parvoviruses) to 280 kilobases (some poxviruses). (msdmanuals.com)
  • Various computational and biochemical methods are used to study protein interfaces. (jove.com)
  • Schneider WM , Chevillotte MD , Rice CM . Interferon-stimulated genes: a complex web of host defenses. (cdc.gov)
  • It acts by binding to RNA and regulating how genes are translated into proteins. (eurekalert.org)
  • They interact with specific intracellular receptor proteins in the target tissues and alter the expression of genes to corticosteroids. (bvsalud.org)
  • This process is mediated by a complex molecular machinery known as the spliceosome, which enables the production of multiple and functionally distinct proteins from single genes. (lu.se)
  • An influenza virus' RNP is composed of both RNA and protein. (cdc.gov)
  • We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
  • Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
  • RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
  • Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
  • To further characterize the function of DJ-1, we cloned cDNA encoding a novel DJ-1-binding protein, DJBP, by a yeast two-hybrid system. (nih.gov)
  • Using the N36S and R63K She2p mutants, we observed that in the absence of She2p RNA-binding activity, neither Myo4p nor She3p is asymmetrically sorted to daughter cells. (elsevierpure.com)
  • New methods for protein-RNA and protein-DNA binding. (bsc.es)
  • Binding to a complex of RNA and protein. (yeastgenome.org)
  • It is still not known which RBPs or adaptor proteins are essential to recruit microtubule-binding motor proteins to mRNPs and to which extend this varies between different transported mRNAs. (europa.eu)
  • The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including PRKRA, TRBP, and Dicer. (hindawi.com)
  • PRKRA is contained in the cytoplasmic RNA-induced silencing complex (RISC) together with transactivation response RNA binding protein (TRBP) and Dicer [ 4 - 6 ]. (hindawi.com)
  • Pigs were treated with 2.25 mg/kg/d recombinant human IGF-1/IGF binding protein-3 complex from birth until day 5 or 9 before the collection of brain samples for quantitative immunohistochemistry (IHC), RNA sequencing, and quantitative PCR analyses. (lu.se)
  • Co-immunoprecipitation of proteins and yeast two-hybrid screening are widely used to provide evidence on whether two proteins interact in vitro . (jove.com)
  • It is thought that motor or adaptor proteins recognise different features on the surface of mRNPs with different affinities. (europa.eu)
  • The RNA-induced silencing complex, or RISC, is a multiprotein complex, specifically a ribonucleoprotein, which functions in gene silencing via a variety of pathways at the transcriptional and translational levels. (wikipedia.org)
  • Multi-scale modeling of macro-complexes, such as transcriptional complexes, nucleosome or chromatin. (bsc.es)
  • Following the demonstration of transcriptional repression by the nucleosome, I have focused on the relief of repression by chromatin-remodeling, especially by the abundant, essential, RSC complex. (stanford.edu)
  • In line with our previous results, we found that there are marked differences between HD patients and controls at the protein level but not at the transcriptional level. (lu.se)
  • They adopt complex dynamic behavior and their functions are directly linked to the arrangement of atoms in 3D (structure) and dynamics. (loria.fr)
  • However, Y-complex proteins show distinguishable protein dynamics, and the deletion of yaaT or ylbF shows a minor effect on the dynamics of YmcA. (uni-marburg.de)
  • Bin/Amphiphysin/Rvs (BAR) proteins are peripheralmembrane proteins (PMP) and play an important role in sculpturingmembranes and in the regulation of actin dynamics. (kth.se)
  • Our goal is to understand RNA function in terms of molecular structure and dynamics using a variety of biophysical and biological tools. (stanford.edu)
  • It is thought that the Y-complex acts as an accessory factor in RNase Y regulation but might also have independent functions. (uni-marburg.de)
  • Using single-stranded RNA (ssRNA) fragments, such as microRNA (miRNA), or double-stranded small interfering RNA (siRNA), the complex functions as a key tool in gene regulation. (wikipedia.org)
  • Size exclusion chromatography confirmed thepresence lncRNA-PC complex in vitro. (kth.se)
  • Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)