Complexes involving rnas. Medical search. Frequent questions

This RNA attaches (binds) to several proteins, forming an enzyme complex called mitochondrial RNA-processing endoribonuclease, or RNase MRP. (medlineplus.gov)
As far as humans are concerned, the argonaute protein Ago-2 is the key cellular binding partner of miRNAs: The Ago-miRNA complex binds to mRNA and impedes their translation into proteins - either by blocking the translation process or by initiating RNA decomposition. (mpg.de)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
The antisense RNA within active RISC binds to its mRNA target, which is then cleaved and degraded by the RNase enzyme in the complex. (news-medical.net)

They include coding mRNAs and non-coding (nc) RNAs among them antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), micro-RNAs (miRNAs), small activating RNAs (saRNAs), RNA aptamers and RNA guides. (frontiersin.org)
MicroRNAs (miRNAs) are master regulators involved in multiple physiological and pathological processes. (genengnews.com)
The two main classes of RNA regulators are miRNAs and RNA-binding proteins. (unibas.ch)
But they also have important regulatory functions: Small noncoding RNAs (miRNAs) influence mRNA stability and are able to switch off genes by stalling their translation into proteins. (mpg.de)
However, miRNAs can't shut off genes on their own: They need to form complexes with other proteins. (mpg.de)
Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. (genetex.com)
Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and RNASEN, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. (genetex.com)
MicroRNAs (miRNAs) are small non-coding RNAs responsible for posttranscriptional regulation of gene expression. (lu.se)

The single strand of RNA acts as a template for RISC to recognize complementary messenger RNA (mRNA) transcript. (wikipedia.org)
While the first mRNA vaccines against an infectious disease have reached the market ( 1 , 2 ), many other medicinal products with RNA as an active substance (see Table 1 ), either vaccines against non-infectious diseases or non-vaccine drugs, are under development ( 3 - 18 ) or even approved ( 19 - 25 ). (frontiersin.org)
There are currently several products containing RNA on the market, and many are under development, among them mRNA, antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), small activating RNAs (saRNAs), micro-RNAs (mi-RNAs), RNA aptamers and RNA guides. (frontiersin.org)
Predicted to be involved in mRNA splicing, via spliceosome. (nih.gov)
Orthologous to human LSM7 (LSM7 homolog, U6 small nuclear RNA and mRNA degradation associated). (nih.gov)
These naturally occurring, noncoding, single-stranded RNAs (21-25 nucleotides long) base-pair with their target mRNA within the RISC (RNA-induced silencing complex). (genengnews.com)
N6-Methyladenosine (m 6 A), a predominant internal modification of RNA in mammalian cells, has been recognized as having a vital role in mRNA stability, export, translation, splicing, and decay [ 4 ]. (hindawi.com)
While there are a lot of studies concerning miRNA processing, the target mRNA recognition and binding by the Ago-miRNA-complex is only poorly understood", says Gunter Meister, the head of the research group "RNA biology" at the Max Planck Institute. (mpg.de)
Importin 8 interacts with Ago and miRNA and is necessary for the binding of the AgomiRNA-complex to a variety of mRNA targets: In the cytoplasm - i.e. the intracellular space outside the nucleus - it recruits the complex to its target, allowing for efficient and specific gene-silencing. (mpg.de)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
RNAi is an antisense mechanism that involves using RNA to target a specific messenger RNA sequence (mRNA) for degradation. (news-medical.net)

Wang, X., Jia, H., Jankowsky, E. & Anderson, J.T. Degradation of hypomodified tRNA(iMet) in vivo involves RNA-dependent ATPase activity of the DExH helicase Mtr4p. (nature.com)
RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. (nature.com)
Callahan, K.P. & Butler, J.S. TRAMP complex enhances RNA degradation by the nuclear exosome component Rrp6. (nature.com)
The production, processing and degradation of RNAs is modulated by various protein complexes that recognize short sequence motifs in their targets. (unibas.ch)
Drive hypothesis generation to improve clinical success rates for programs involving small molecules, peptides, RNAs, protein degradation, molecular glues, transient covalent inhibitors, and kinetic stabilization of drug-target complexes. (novartis.com)
The TRAMP complex is involved in exosome-mediated degradation of aberrant RNAs and RNA surveillance in the nucleus. (eu.org)
Oligo-adenylated tails are added to aberrant RNA substrates, thus marking them for degradation. (eu.org)
In order to switch off a gene, they interact with so called Argonaute proteins - the subsequent complex induces the shutdown or even degradation of the genetic information. (mpg.de)
Refrigerate samples at 4°C immediately after collection and, if possible, process them within 24 hours to reduce SARS-CoV-2 RNA degradation and increase surveillance utility. (cdc.gov)

Splicing of mammalian precursor transfer RNA (tRNA) molecules involves two enzymatic steps. (nih.gov)
Ribosome synthesis involves transcription of ribosomal RNA genes, processing and assembly of ribosomal precursor molecules in the nucleolus and the nucleus, their transport to the cytoplasm and final maturation. (uni-regensburg.de)
Chemical modifications to provide drug-like properties to RNA molecules are used in the synthesis of both siRNAs and antimicroRNAs (antimiRs). (genengnews.com)
The microarray technology is based on the hybridization of labelled DNA or RNA molecules to complementary sequences that are immobilized on a solid substrate. (tutorialspoint.com)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
If we grant the evolutionists a lot of slack and assume that RNA molecules with meaningful biological information could somehow magically burst onto the scene in a chemical milieu favorable to RNA stability and life, there are still many other problems. (icr.org)
CHOP researchers have developed a versatile and low-cost technology for targeted sequencing of full-length RNA molecules. (chop.edu)

This was only a couple of years after the discovery of RNA interference in 1998 by Andrew Fire and Craig Mello, who shared the 2006 Nobel Prize in Physiology or Medicine. (wikipedia.org)
The RNase III Dicer is a critical member of RISC that initiates the RNA interference process by producing double-stranded siRNA or single-stranded miRNA. (wikipedia.org)
RNA interference-mediated depletion of HSPC117 inhibited maturation of intron-containing pre-tRNA both in vitro and in living cells. (nih.gov)
Some of the same strategies already developed for delivery of siRNA for RNA interference (RNAi) also are being applied to miRNA. (genengnews.com)
2062 : 127-145 Global Identification of Human Exosome Substrates Using RNA Interference and RNA Sequencing. (genetex.com)
Isis Pharmaceuticals, Inc. has announced that it has been awarded a multi-year Phase 2 Small Business Innovation Research (SBIR) grant by the National Institutes of Health (NIH) for up to $1.5 million to design oligonucleotide drugs that can exploit the RNA interference (RNAi) antisense mechanism for disease treatment. (news-medical.net)

Static and kinetic site-specific protein-DNA photocrosslinking has major implications for transcription in general, for the topology determinants specifically for RNA polymerase II transcription, and the mechanistic determinants of contacts involved in nucleoprotein complexes. (rsc.org)

Sloan, K.E., Bohnsack, M.T., Schneider, C. & Watkins, N.J. The roles of SSU processome components and surveillance factors in the initial processing of human ribosomal RNA. (nature.com)
This complex process is evolutionary conserved and involves several RNA processing and modification steps required for correct ribosomal RNA maturation. (rcsb.org)
We recently showed that a member of the mitochondrial transcription termination factor (MTERF) family of proteins, MTERF4, recruits NSUN4, a 5-methylcytosine RNA methyltransferase, to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. (rcsb.org)
This study provides a molecular explanation for MTERF4-dependent recruitment of NSUN4 to ribosomal RNA and suggests a unique mechanism by which other members of the large MTERF-family of proteins can regulate ribosomal biogenesis. (rcsb.org)

Here, single-molecule high-resolution optical trapping measurements reveal that Mtr4p unwinds RNA duplexes by 3′-to-5′ translocation on the loading strand, that strand separation occurs in discrete steps of 6 base pairs and that a single Mtr4p molecule performs consecutive unwinding steps. (nature.com)
Instead, a molecule called a noncoding RNA, a chemical cousin of DNA, is produced from the RMRP gene. (medlineplus.gov)
This unstable molecule cannot bind to some of the proteins needed to make the RNase MRP enzyme complex. (medlineplus.gov)
Life requires DNA, RNA, and protein in an interdependent triad in which each molecule is wholly dependent on the other two to exist. (icr.org)
Furthermore, since each type of molecule carries and conveys complex encoded information, an intelligent information provider is the only logical cause of this information source. (icr.org)
One of these additional obstacles is how the first RNA molecule could have possibly replicated itself without the aid of protein polymerases. (icr.org)

Hannon and his colleagues attempted to identify the RNAi mechanisms involved in gene silencing, by dsRNAs, in Drosophila cells. (wikipedia.org)
We are pleased with the continued support from the NIH in our ongoing efforts to apply our RNA technologies, oligonucleotide chemistries and expertise to exploit antisense mechanisms, such as RNAi, for therapeutic benefit. (news-medical.net)
The most common RNAi agents are double-stranded RNAs, which ultimately associate with proteins to form an RNA-induced silencing complex (RISC). (news-medical.net)
Although most currently practiced methods of inducing the RNAi mechanism utilize double-stranded RNAs, RISC requires only the antisense strand to function. (news-medical.net)

The main lines of our work are 1) reconstitution of the process with more than 50 pure proteins and mechanistic analysis, 2) structure determination of the 50 protein complex at atomic resolution, and 3) studies of chromatin remodelling, required for transcription of the DNA template in living cells. (stanford.edu)
Following the demonstration of transcriptional repression by the nucleosome, I have focused on the relief of repression by chromatin-remodeling, especially by the abundant, essential, RSC complex. (stanford.edu)
They form into a protein complex that has helicase activity and is involved in a variety of DNA-related functions including replication elongation, RNA transcription, chromatin remodeling, and genome stability. (bvsalud.org)

RNA helicase activity of Mtr4p is critical for biological functions of the enzyme, but the molecular basis for RNA unwinding is not understood. (nature.com)
Our goal is to understand RNA function in terms of molecular structure and dynamics using a variety of biophysical and biological tools. (stanford.edu)
But in 2001, he and his colleagues published the first molecular snapshot of RNA polymerase in action. (chicagotribune.com)
Our goal is to understand the molecular mechanisms whereby the genomic RNA of influenza-like viruses is, on the one hand, the template for transcription and replication of the viral genome by its RNA-dependent RNA polymerase and, on the other hand, an Achilles' heel, whose recognition as non-self can trigger an innate immune response to counter the viral infection. (embl.org)
The identification of causal or predictive variants/genes/mechanisms for disease-associated traits is characterized by "complex" networks of molecular phenotypes. (igi-global.com)
Photochemical and photophysical processes in biomolecules are intimately involved in a multitude of functional processes, that include vision, photosynthesis, molecular recognition, gene replication, etc., and can be utilized in areas such as photodynamic therapy. (rsc.org)
Along with measurement of SARS-CoV-2 RNA in this step, several laboratory controls should also be measured, including matrix recovery controls, human fecal normalization, quantitative measurement controls, and controls to assess molecular method inhibition. (cdc.gov)
To cap- lular phenotypes (e.g. disease) on a mechanistic level, and ture the complex network of nonlinear information process- to use genomic signals to classify disease on a molecular ing based upon multivariate inputs from inside and outside level. (lu.se)

This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. (nih.gov)
Predicted to enable RNA polymerase II-specific DNA-binding transcription factor binding activity. (jax.org)
For more than 30 years, Kornberg has been studying a process known as transcription, in which genetic information contained in DNA in a cell's nucleus is translated into messenger RNA. (chicagotribune.com)
Transcription is crucial not only in keeping each cell alive, but also in determining which of the 30,000 genes in every human cell will be used and thus whether a cell will become a muscle cell, a nerve fiber or any of the myriad other types required by a complex organism. (chicagotribune.com)
The transcription process is carried out by an enzyme called RNA polymerase. (chicagotribune.com)
These studies are being extended to viral RNPs (the physiological RNA synthesis units) to understand the behaviour of the viral nucleoprotein during replication and transcription and to include host factors important for viral replication. (embl.org)
TAF9 is a TATA-binding protein associated factor (TAF) conserved from yeast to humans and shared by two transcription coactivator complexes, TFIID and SAGA. (thebiogrid.org)
Retroviruses are enveloped RNA viruses defined by their mechanism of replication via reverse transcription to produce DNA copies that integrate into the host cell's genome. (msdmanuals.com)

Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)

The RNA-induced silencing complex, or RISC, is a multiprotein complex, specifically a ribonucleoprotein, which functions in gene silencing via a variety of pathways at the transcriptional and translational levels. (wikipedia.org)
Major proteins of RISC, Ago2, SND1, and AEG-1, act as crucial contributors to the gene silencing function of the complex. (wikipedia.org)

The Jardetzky Laboratory is studying the structures and mechanisms of macromolecular complexes important in viral pathogenesis, allergic hypersensitivities and the regulation of cellular growth and differentiation, with an interest in uncovering novel conceptual approaches to intervening in disease processes. (stanford.edu)
We are interested primarily in mechanisms of post-transcriptional gene expression control, involving small non-coding RNAs and RNA-binding proteins. (unibas.ch)
RNA processing and surveillance is a mechanisms closely regulated. (eu.org)

respiratory burst (the rapid release of Reactions of oxygen radicals with Mitochondrial oxidative phosphor- reactive oxygen species from cells) cel ular components can deplete an- ylation is a major source of oxy- that involves nicotinamide adenine tioxidants, can cause direct oxidative gen radicals of endogenous origin. (who.int)

Macromolecular complex annotations are imported from the Complex Portal . (yeastgenome.org)
J Med Chem 2005) and extended it to macromolecular complexes involving proteins and protein-RNA complexes beyond topoisomerases (Pommier et al. (cancer.gov)

The RNase MRP enzyme is thought to be involved in several important processes in the cell. (medlineplus.gov)
RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)
This enzyme is essential for conversion of the viral RNA to proviral DNA, which integrates in the host DNA and results in viral replication. (who.int)

The Cusack group uses X-ray crystallography and cryo electron-microscopy (cryoEM) to study the structural biology of protein-RNA complexes involved in RNA virus replication, innate immunity and cellular RNA metabolism. (embl.org)
In parallel, we are doing the same for viral replication, which is unprimed and occurs in two-steps via an intermediate complementary RNA (cRNA). (embl.org)
Peptides are merely really short versions of proteins, so the scientists essentially cheated and the RNA replication process was not exclusively RNA-based. (icr.org)
2016. Oligoarginine peptides slow strand annealing and assist non-enzymatic RNA replication. (icr.org)

Using single-stranded RNA (ssRNA) fragments, such as microRNA (miRNA), or double-stranded small interfering RNA (siRNA), the complex functions as a key tool in gene regulation. (wikipedia.org)
Building on such data, we have proposed the first biophysical model of miRNA-target RNA interaction , a model that predicts the affinity of such interactions with high accuracy . (unibas.ch)
Now his group has identified the first protein factor which is required for gene-silencing by Ago-miRNA-complexes: Importin 8. (mpg.de)
Indeed the scientists proved that Importin 8 is involved in the transport of Ago-miRNA-complexes into the nucleus. (mpg.de)
I was involved in the first study published in 2004 describing an islet specific miRNA, miR-375. (lu.se)
Through the centre, we have access to human islets from which we have investigated the miRNA-profile and together we perform large-scale RNA-sequencing studies in human islets that has given us the opportunity to investigate other non-coding RNAs such as the lncRNAs and their role in beta cell function. (lu.se)

De Novo Variants in CNOT1, a Central Component of the CCR4-NOT Complex Involved in Gene Expression and RNA and Protein Stability, Cause Neurodevelopmental Delay. (bvsalud.org)
CNOT1 is a member of the CCR4-NOT complex, which is a master regulator, orchestrating gene expression , RNA deadenylation, and protein ubiquitination . (bvsalud.org)
In this section, we comprehensively analyzed the expression levels of fourteen m 6 A RNA methylation regulators and clinical factors in patients with HCC from the ICGC (International Cancer Genome Consortium, https://icgc.org/ ), Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/ ), and TCGA (The Cancer Genome Atlas, http://cancergenome.nih.gov/ ) databases. (hindawi.com)
In eukaryotes, gene expression is a complex process, with many steps, all amenable to regulation. (unibas.ch)

Sci Rep. 2018) and nucleoside analogue, RNA synthesis inhibitors (Kouba et al . (embl.org)
The genes encoding RBPs and the relevant genes are involved in the transformation from PM to PTM, then constructing the TM by regulating protein synthesis. (peerj.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)

Most of the m 6 A RNA methylation regulators were confirmed to be differentially expressed among groups stratified by clinical characteristics and tissues. (hindawi.com)
In summary, we demonstrated the vital role of m 6 A RNA methylation regulators in the initial presentation and progression of HCC and constructed a nomogram which would predict the clinical outcome and provide a basis for individualized therapy. (hindawi.com)
To this end, we develop models to explain various properties of RNAs, such as the usage of specific processing sites or the expression level of individual isoforms in terms of the combination of binding sites that these RNAs have for various regulators. (unibas.ch)
This enabled us to identify novel regulators of RNA processing. (unibas.ch)

The following graphic depicts a typical workflow for preparing, analyzing, and visualizing results from transcriptomic RNA sequencing data. (r-bloggers.com)

It is a computational field that involves the analysis of complex omics data. (r-bloggers.com)

USP15 Deubiquitinates TUT1 Associated with RNA Metabolism and Maintains Cerebellar Homeostasis. (nih.gov)

The International Workshop on RNA Informatics (IWRI 2020) is the eleventh in a series of workshops that aim at providing an international forum to discuss the most recent developments in the field regarding application of informatics on noncoding RNAs, RNA modifications including methylation, and other related work. (wikicfp.com)
We identified an m 6 A RNA methylation regulator-based signature (including METTL3, YTHDC2, and YTHDF2) that could effectively stratify a high-risk subset of these patients by univariate and LASSO Cox regression, and receiver operating characteristic (ROC) analysis indicated that the signature had a powerful predictive ability. (hindawi.com)

Since requires its own model systems, not simply straightforward transcriptional (and posttranscriptional) control involves the adaptations of currently formulated models. (lu.se)

The scope of this workshop is to use machine learning, deep learning, knowledge graph, and other genome technology and informatics methods to contribute to the understanding of the structure, function, evolution, comparative genomics, and regulation of noncoding RNAs and RNA modifications. (wikicfp.com)
Predicted to act upstream of or within regulation of protein-containing complex assembly. (jax.org)
Specific gene-silencing is involved in the development of cancer and plays an essential role in gene regulation. (mpg.de)
Possibly it is involved in gene regulation, too. (mpg.de)

Using activity-guided purification of tRNA ligase from HeLa cell extracts, we identified HSPC117, a member of the UPF0027 (RtcB) family, as the essential subunit of a tRNA ligase complex. (nih.gov)

van Hoof, A., Lennertz, P. & Parker, R. Yeast exosome mutants accumulate 3′-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs. (nature.com)

A structural study on the coronavirus main protease 3CL pro inhibitor complex established designing of broad-spectrum halomethyl ketone inhibitors to the Coronaviridae family and demonstrated that these inhibitors form a thioether linkage with high affinity to the target 3 . (nature.com)
Hilgenfeld's group reported earlier that a structure-based design of peptidomimetic α-ketoamides are also effective broad-spectrum inhibitors to the main and 3C protease of coronaviruses and enteroviruses as demonstrated by crystal structure of inhibitor-protease complex 4 . (nature.com)
Dr. Pommier conceptualized the "interfacial inhibitors" mechanism based on his finding that DNA topoisomerase inhibitors act by trapping topoisomerase-DNA complexes (Capranico et al. (cancer.gov)
The activity of these XCI-activators is counterbalanced by autosomally encoded XCI-inhibitors, many of which are pluripotency factors involved in repression of Xist , providing a powerful link between loss of pluripotency and XCI initiation. (erasmusmc.nl)

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. (nih.gov)
The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. (nih.gov)

We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
Based on either 3' end or full-length RNA sequencing data, we infer the relative usage of each poly(A) site in a given terminal exon. (unibas.ch)
Pigs were treated with 2.25 mg/kg/d recombinant human IGF-1/IGF binding protein-3 complex from birth until day 5 or 9 before the collection of brain samples for quantitative immunohistochemistry (IHC), RNA sequencing, and quantitative PCR analyses. (lu.se)
However, classifying patients as BCP with RNA sequencing did make a difference. (medscape.com)

The expression levels of genes involved in neuronal and oligodendrocyte maturation, and angiogenic and transport functions were al-tered, reflecting enhanced brain maturation in response to IGF-1 treatment. (lu.se)

Ribonucleic acids (RNAs) carry as messenger-RNAs (mRNAs) genetic information from DNA to cellular protein factories, where they are translated into proteins. (mpg.de)

HIV infection can be diagnosed by antibody, nucleic acid (HIV RNA), or antigen (p24) testing. (msdmanuals.com)

Analysis of protein - protein interactions with other members indicated that the CCR4-NOT complex remained intact. (bvsalud.org)
We explore the intricate structural interactions between viruses and host complexes during virus infection and immune response, which is crucial for exploiting them and uncovering new avenues for the development of therapeutics. (gla.ac.uk)
Cis and trans interactions between genes encoding PAF1 complex and ESCRT machinery components in yeast. (thebiogrid.org)
Knowing where RBPs bind gives us the opportunity to investigate the impact of these interactions on the target RNAs. (unibas.ch)

The cell counters RNA viruses with innate immune pattern-recognition receptors, such as the RNA helicase RIG-I, which recognise particular viral RNA structural motifs (e.g. 5′ triphosphate-dsRNA) as non-self, thus activating a signalling pathway leading to interferon production and establishment of the anti-viral state. (embl.org)
We have extended our work on viral polymerases to those of other segmented negative-strand RNA viruses such as the large order of Bunyavirales . (embl.org)

Telomerase RNA biogenesis involves sequential binding by Sm and Lsm complexes. (stowers.org)
The main objective of our research is the complex process of eukaryotic ribosome biogenesis. (uni-regensburg.de)
This study is to explore the biogenesis mechanism of a secondary hepatocellular carcinoma (HCC) based on the function of RNA binding proteins (RBPs)-encoding genes in the physiological microenvironment (PM). (peerj.com)

it is a key process in defense against viral infections, as it is triggered by the presence of double-stranded RNA (dsRNA). (wikipedia.org)

5 Small chains of amino acids called peptides were used to help keep the products of the replicated short RNAs from binding to each other. (icr.org)
So not only did this so-called RNA World study cheat by using peptides, meaning it really wasn't just an RNA World, but the research was misinterpreted and unrepeatable. (icr.org)

We further show that RNA unwinding by Mtr4p requires interaction with upstream RNA duplex. (nature.com)
Unlike SARS-CoV-2, SARS-CoV M pro dimer has a polar interaction between the two domains III involving a 2.60 Å hydrogen bond between the side chain hydroxyl groups of Thr 285 of each protomer which is also supported by a hydrophobic interaction between the side chain of Ile 286 and Thr 285 . (nature.com)
however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. (genetex.com)

The conserved Saccharomyces cerevisiae Ski2-like RNA helicase Mtr4p plays essential roles in eukaryotic nuclear RNA processing. (nature.com)
The high sequence conservation of HSPC117/RtcB proteins is suggestive of RNA ligase roles of this protein family in various organisms. (nih.gov)
The RNAs play important roles in the pathological and physiological tissues including cancer. (peerj.com)

However, although many thousands of alternatively spliced transcripts are routinely detected in RNA-seq studies, reliable large-scale mass spectrometry-based proteomics analyses identify only a small fraction of annotated alternative isoforms. (blogspot.com)
This is especially interesting, because over the past years there have been controversial discussions as to whether or not small noncoding RNAs occur in the nucleus. (mpg.de)
For example, if levels of SARS-CoV-2 RNA are sufficiently high in wastewater, small volumes of wastewater (e.g., 1 ml) may be tested without additional concentration processes. (cdc.gov)

Anderson, J.T. & Wang, X. Nuclear RNA surveillance: no sign of substrates tailing off. (nature.com)
While Air1 or Air2 recognizes the RNA substrates, Mtr4 is responsible for unwinding the RNA in order for Trf4 or Trf5 to polyadenylate the substrate ( LaCava,2005 ). (eu.org)

However, nucleotides are much more complex than the simple amino acids made by Miller and Urey, and would require much more chemical expertise to produce. (bibliotecapleyades.net)
Furthermore, Mtr4 can modulate the activity of Trf4/5 in the TRAMP complex, resulting in a poly(A) tail length of only 3-5 nucleotides ( Jia,2011 ). (eu.org)

This commonly includes DNA, RNA, or protein sequence data. (r-bloggers.com)
For RNA-binding proteins (RBPs), both in vivo and in vitro binding data has been generated, but principled models describing the sequence specificity of RBPs are yet to be developed. (unibas.ch)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)

The RNA helicase Mtr4p modulates polyadenylation in the TRAMP complex. (nature.com)

The TRAMP complex consists of a RNA helicase (MTR4), a poly(A)polymerase (Trf4 or Trf5) and a RNA-binding zinc knuckle protein (Air1 or Air2). (eu.org)

The Puglisi group investigates the role of RNA in cellular processes and disease. (stanford.edu)
A long-term goal is to target processes involving RNA with novel therapeutic strategies. (stanford.edu)
The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved. (yeastgenome.org)
In sharp contrast, however, information on electronic processes in biomolecules such as isolated proteins and DNA (and their complexes) is still in its infancy. (rsc.org)

and its complex with α-ketoamide inhibitor after a modification from the previously designed inhibitor through incorporating P3-P2 amide bond into pyridone ring so as to increase its half-life in plasma. (nature.com)
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, and N6-methyladenosine (m 6 A) is a predominant internal modification of RNA in various cancers. (hindawi.com)

In animals, the second step predominantly entails direct exon ligation by an elusive RNA ligase. (nih.gov)

In this study, we examined the interplay between genes encoding components of the PAF1 complex and VPS36, the gene located next to CDC73 on chromosome XII. (thebiogrid.org)

Whereas the underlying pathophysiological mechanism warrants further analysis , our data demonstrate an essential and central role of the CCR4-NOT complex in human brain development. (bvsalud.org)
Inclusion of Mtr4p within the TRAMP complex increases the rate constant for unwinding initiation but does not change the characteristics of Mtr4p's helicase mechanism. (nature.com)

In Fungi , it is formed by a RNA Helicase, Mtr4, in addition with a poly(A)polymerase, Trf4 or Trf5 and a RNA-binding zinc knuckle protein, Air1/2. (eu.org)

This Xic covers a region of ~800kb, and harbors a plethora of long non-coding RNA (lncRNA) genes involved in XCI. (erasmusmc.nl)

In fact, a secular scientist published a 2012 paper expressing this great frustration titled, "The RNA world hypothesis: the worst theory of the early evolution of life (except for all the others). (icr.org)
Bernhardt, H. S. 2012 The RNA world hypothesis: the worst theory of the early evolution of life (except for all the others). (icr.org)

Anatomy19

Organisms41

Chemicals and Drugs98

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes81

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science6

Binds4

MiRNAs8

MRNA13

Degradation9

Molecules7

Interference6

Polymerase II transcription1

Ribosomal4

Molecule6

RNAi4

Chromatin3

Molecular8

Transcription8

Antisense2

RISC2

Mechanisms3

Mitochondrial1

Macromolecular2

Enzyme3

Replication4

MiRNA6

Gene expression4

Synthesis3

Regulators4

Transcriptomic1

Computational1

Metabolism1

Methylation2

Transcriptional1

Regulation4

TRNA1

Nucleolar1

Inhibitors4

Genome2

Sequencing4

Maturation1

MRNAs1

Nucleic acid1

Interactions4

Viruses2

Biogenesis3

Double-stranded1

Peptides2

Interaction3

Roles3

Small3

Substrates2

Nucleotides2

Sequence3

Modulates1

Air21

Processes4

Modification2

Exon1

Paf11

Mechanism2

Mtr41

Genes involved1

Hypothesis2