This enzyme catalyses the following chemical reaction acetoin + CoA + NAD+ ⇌ {\displaystyle \rightleftharpoons } acetaldehyde + acetyl-CoA + NADH + H+ This enzyme requires thiamine diphosphate. (wikipedia.org)
The enzyme was NAD(H)-specific and its optimal activity for acetoin reduction was observed at pH 6.5 and 55 °C. The optimal pH and temperature for 2,3-butanediol oxidation were pH 10 and 45 °C, respectively. (mdpi.com)
3. Pahlich, E. and Joy, K.W. Glutamate dehydrogenase from pea roots: purification and properties of the enzyme. (qmul.ac.uk)
For example, the limiting step and the control point for alginate biosynthesis is the activity of the GDP-mannose dehydrogenase enzyme, which is involved in the irreversible oxidation of GDP-mannose to GDP-mannuronic acid [ 24 ], and is NAD + -dependent [ 25 ]. (biomedcentral.com)
Irreversible inhibitors first form a reversible non-covalent complex with the enzyme (EI or ESI). (cdk-receptor.com)
Butanediol dehydrogenase2
The gene encoding a (2 R ,3 R )-2,3-butanediol dehydrogenase from Rhodococcus erythropolis WZ010 (ReBDH) was over-expressed in Escherichia coli and the resulting recombinant ReBDH was successfully purified by Ni-affinity chromatography. (mdpi.com)
Searching for up to 100 curated homologs for 201943 SO2813 oxidoreductase, short chain dehydrogenase/reductase family (NCBI ptt file) (254 a.a. (lbl.gov)
This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). (thermofisher.com)
Pyruvate1
1987 ) Isolated tumoral pyruvate dehydrogenase can synthesize acetoin which inhibits pyruvate oxidation as well as other aldehydes. (academictree.org)
1986 ) Inhibitors of the mitochondrial cytochrome b-c1 complex inhibit the cyanide-insensitive respiration of Trypanosoma brucei. (academictree.org)
Single deletions of the genes encoding for the glycerol/H + symporter ( STL1 ), the glycerol kinase ( GUT1 ), and the mitochondrial FAD + -dependent glycerol 3-phosphate dehydrogenase ( GUT2 ) abolished glycerol growth in this strain, implying that it uses the same glycerol utilization pathway as previously identified in auxotrophic laboratory strains. (biomedcentral.com)
Characterization1
1. Olson, J.A. and Anfinsen, C.B. The crystallization and characterization of L -glutamic acid dehydrogenase. (qmul.ac.uk)
NADP1
At the highest OTR condition, a decrease in the NADPH/NADP + ratio of threefold was observed, probably as a response to the high respiration rate induced by the respiratory protection of the nitrogenase under diazotrophic conditions, correlating with a high expression of the uncoupled respiratory chain genes ( ndhII and cydA ) and induction of the expression of the genes encoding the nitrogenase complex ( nifH ). (biomedcentral.com)
The purified ReBDH in the native form was found to exist as a monomer with a calculated subunit size of 37180, belonging to the family of the zinc-containing alcohol dehydrogenases. (mdpi.com)
Protein2
They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria. (lookformedical.com)
Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. (thermofisher.com)
Substrate1
1. O'Connor, R.J. and Halvorson, H. The substrate specificity of L -alanine dehydrogenase. (qmul.ac.uk)
Alcohol1
A further improvement of the maximum butanol titer was achieved using an engineered L. diolivorans strain overexpressing the endogenous alcohol dehydrogenase pduQ. (biomedcentral.com)
â £In terms of cognition of high-risk prognostic factors, physicians' knowledge of unmutated immunoglobulin heavy-chain variable and 11q- is far inferior to that of TP53 mutation and complex karyotype, which are two high-risk prognostic factors, and only 17.1% of the interviewed physicians fully mastered CLL International Prognostic Index scoring system. (bvsalud.org)
Diacetyl2
Other compounds detected in area and personal air samples included substitutes for diacetyl and 2,3-pentanedione (acetoin and 2,3-hexanedione) and compounds that may be contaminants or impurities. (cdc.gov)
BFV are a complex mixture of volatile agents containing of diacetyl and butyric acid, two components of BFV, and to develop a hybrid computational fluid dynamic-physiologically diacetyl (2,3-butanedione), acetoin, 2-nonanone, acetic acid, based pharmacokinetic model (CFD-PBPK) to describe these and butyric acid, among other vapors (Boylstein et al. (cdc.gov)
Pseudomonas1
The crystal structure of a heterotetrameric (alpha2beta2) E1, 2-oxoisovalerate dehydrogenase from Pseudomonas putida, reveals a tightly packed arrangement of the four subunits with the beta2-dimer held between the jaws of a 'vise' formed by the alpha2-dimer. (nih.gov)
Data1
The complex, in combination with mutagenesis and kinetic data, provides a structural basis for understanding the mechanism of IMPDH activity and indicates that MPA inhibits IMPDH by acting as a replacement for the nicotinamide portion of the nicotinamide adenine dinucleotide cofactor and a catalytic water molecule. (embl.de)
Active1
The latter components regulate enzymatic activity by interconverting the dehydrogenase between active (nonphosphorylated) and inactive (phosphorylated) forms involving three specific serine residues that participate in TPP binding. (ferienwohnung-gluecksburg.net)