• moreover, commensal vaginal flora or dental plaque bacteria may create such complex microbial consortia. (am-online.org)
  • While the impact of the farming system on microbial community composition has been extensively reported in the literature, whether sustainable farming approaches can promote associations between bacteria and fungi is still unclear. (bvsalud.org)
  • We are developing and applying high-throughput single-cell genomic technologies to identify and characterize diverse functional subpopulations of bacteria within complex microbial samples. (isbscience.org)
  • We are interested in unraveling the roles and behavior of individual bacteria in challenging environments such as the human host, and within structured multi-species communities known as biofilms. (isbscience.org)
  • Bacteria differentiate into distinct subpopulations responsible for different functional activities to benefit the community. (isbscience.org)
  • Given the complexity observed even in lab-grown monocultures, this heterogeneity is likely further magnified within complex environments and in the presence of other species, where bacteria employ bet-hedging and labor division strategies to enhance their fitness. (isbscience.org)
  • Microbiota, complex consortia of bacteria, are found in all environments, including the human body. (isbscience.org)
  • Süel's team recently discovered that bacteria, specifically those who employ the use of biofilms, which are groups of bacterial cells adhered to one another, use electric signaling to communicate, and even 'advertise' their social community to recruit other bacteria. (ucsd.edu)
  • Analogous to how a club distributes flyers to passersby on Library Walk, electric signals attempt to convince other species of bacteria to become a part of the biofilm. (ucsd.edu)
  • These bacteria, previously thought to be simple and limited in function as a result of their single-celled nature, have demonstrated incredibly complex social interactions and community structures. (ucsd.edu)
  • Prior to Dr. Süel and his team's findings, it was thought that these biofilms were just simple aggregations of several kinds of bacteria. (ucsd.edu)
  • The electric signals sent out by the biofilm have a wide range of control over the actions of the receiving bacteria, over a wide range of species. (ucsd.edu)
  • So why is this interspecies electrical communication between biofilms and independent bacteria important to us? (ucsd.edu)
  • Bacteria is the source of many infections, and biofilms are major functioning components in the human body. (ucsd.edu)
  • Microbial electrical signaling is a way in which we could have trillions of bacteria suddenly working for us. (ucsd.edu)
  • the bacteria organize themselves into a coordinated functional community. (wikipedia.org)
  • [13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
  • They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
  • [8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
  • Bacteria often switch from a free-living lifestyle to a surface adapted, structured lifestyle known as a biofilm. (southampton.ac.uk)
  • We found that subpopulations of biofilm bacteria undergo cell death and lysis as a feature of the Pseudomonas aeruginosa biofilm life cycle. (southampton.ac.uk)
  • We isolated and named this phage, showed that it becomes superinfective to its host during biofilm development, and found that it plays a role in phenotypic diversification of biofilm bacteria. (southampton.ac.uk)
  • In the human body, microbes such as bacteria and fungi, exist as complex, multicellular communities known as biofilms. (tigs.res.in)
  • Competitive interactions also abound in microbial ecosystems, with examples ranging from virus-bacteria and protist-bacteria predator-prey interactions to inter-species bacterial warfare. (ncbs.res.in)
  • While homogeneous planktonic populations were studied in sterile labs, the heterogeneous (mixed bacteria, virus, fungus) biofilms represent reality in our body. (blogspot.com)
  • The extracellular polymeric substance (EPS), also called 'slime', covering and cementing of biofilm colonies protects the bacteria from antibiotics, bacteriocins (produced by probiotics), antibodies, and other medicines/herbs which might kill it. (blogspot.com)
  • Multispecies Outbreak of Verona Integron-Encoded Metallo-ß-Lactamase-Producing Multidrugresistant Bacteria Driven by a Promiscuous Incompatibility Group A/C2. (cdc.gov)
  • The study intends to explore the therapeutic potential of naturally produced compounds by competitive marine bacteria residing in marine biofilms against C. albicans biofilm. (solamonrenewableenergy.com)
  • Several independent studies have shown that surface-associated bacteria produce bioactive compounds with clinical importance, including antibiotics and anti-biofilm agents8,9,10. (solamonrenewableenergy.com)
  • Therefore, biofilm matrix gives the additional resistance power to bacteria which makes them to not only tolerate harsh conditions but also resistant to antibiotics which lead to the emergence of bad bugs infections like multi drug resistant, extensively drug resistant and totally drug resistant bacteria. (biomedcentral.com)
  • Marine bacteria from the French Atlantic coast displaying high forming-biofilm abilities and different biofilm 3D architectures. (univ-ubs.fr)
  • Dysbiotic communities are characterized by increased numbers of bacteria that exploit the serum-like transudate for nutrients, giving rise to a proteolytic community phenotype. (biomedcentral.com)
  • 6. Multi-species biofilms and the development of infections. (am-online.org)
  • Understanding the physiology of these structured microbial communities is not only essential to the treatment of chronic infections, but also important to industrial applications, such as bioremediation, anti-biofouling and food hygiene. (nature.com)
  • The clinical relevance of multi-species biofilms is apparent, ranging from the relatively innocuous dental plaque, to limb-threatening wound infections, and life-threatening lung and medical device infections. (tigs.res.in)
  • Given this, there is an increased push to incorporate host relevant components in biofilm studies, that can closely mimic clinical conditions and provide hitherto unexplored insights into biofilm infections [2]. (tigs.res.in)
  • In wounds, biofilms are most often mixed-species infections, and Staphylococcus aureus and Pseudomonas aeruginosa are the most common co-pathogens. (tigs.res.in)
  • In contrast to animal models of biofilm infections, these platforms offer the advantages of selective and precise control, in an ethical and cost-effective manner. (tigs.res.in)
  • Stealth infections, hiding in biofilm communities, were never mentioned. (blogspot.com)
  • Although Anthony van Leeuwenhoek, who discovered microbial attachment to his own tooth surface, is credited with the discovery of biofilm nearly 40 years ago, one of the major breakthroughs happened when Dr. G.D. Ehrlich confirmed the hypothesis in 2006, that indeed chronic middle-ear disorders are 'chronic' biofilm related and don't recur due to new infections. (blogspot.com)
  • Today we know that biofilms are involved in most (probably all) microbial, and even some viral or mixed multispecies (bacterial, viral, fungal) infections in the body. (blogspot.com)
  • It is estimated that 80% of all (and likely 100% of chronic) infections are biofilm related as biofilm formation is the preferred bacterial life style. (blogspot.com)
  • Biofilm formation is the prime cause of antibiotic resistance during C. albicans infections and treating biofilm-forming cells is challenging due to their intractable and persistent nature. (solamonrenewableenergy.com)
  • In conclusion, this study showed that marine biofilms are a hidden source of diverse therapeutic drugs, and 3HC could be a potent drug to treat C. albicans infections. (solamonrenewableenergy.com)
  • Further, virulence traits, including adhesion, hyphal, and biofilm formation enable C. albicans to access deep tissues for systemic infections. (solamonrenewableenergy.com)
  • Therefore, there is a critical need for alternative therapeutic agents to fight against biofilm-mediated infections and to conquer the limitations of current antifungal therapies. (solamonrenewableenergy.com)
  • Confocal microscopy in conjunction with fluorescence in situ hybridization as well as the atomic force microscopy are both very useful techniques to study the three-dimensional structure of the mixed-species biofilms. (am-online.org)
  • These mixed-species biofilms interact with, and are influenced by, various host components such as the wound bed (consisting of skin cells fibroblasts and keratinocytes), presence of wound fluid (a protein-rich drainage), chemical factors (lactic acid, glucose, enzymes) and immune cells (neutrophils, macrophages). (tigs.res.in)
  • What specialized subpopulations emerge in biofilms in response to the environmental stressors such as antimicrobial agents? (isbscience.org)
  • Motile subpopulations in microbial communities are believed to be important for dispersal, quest for food, and material transport. (nature.com)
  • Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)
  • Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
  • This project will exploit novel nitric oxide based therapies to improve the effectiveness of antibiotics against Pseudomonas aeruginosa biofilms. (southampton.ac.uk)
  • Wound biofilms are recalcitrant to conventional antibiotics and immune clearance, which further fuels the chronic infection state. (tigs.res.in)
  • Microorganisms residing within biofilms are highly resistant to antimicrobials including antibiotics and bacteriocins produced by probiotics. (synergisticseurope.com)
  • Turns out that, in most real-world conditions, microbes adopt a far more complex lifestyle. (tigs.res.in)
  • Biofilms are three-dimensional structures, often seen as aggregates of microbes in self-produced polymeric matrix, attached to each other or to a surface. (tigs.res.in)
  • In biofilms, microbes actively communicate with each other via chemical signals that increase with cell density. (tigs.res.in)
  • When I listened to our professors, some 30 years ago, talking about infectious diseases or microbes, biofilms were never mentioned. (blogspot.com)
  • Microbiome research is a rapidly developing area of science and innovation, seeking to explore and exploit the complex communities of microbes associated with humans, animals, plants and other environments such as soils and oceans. (microbiologysociety.org)
  • How to gain and use knowledge about multi-species biofilms? (am-online.org)
  • Multi-species biofilms form in many natural settings: water, soil or even on dust particles in the air. (am-online.org)
  • However, many technical obstacles may occur with these consortia during experimentation, e.g., how to grow multi-species biofilms with high reproducibility regarding the quantitative composition of these communities, and whether it is possible to quantitative by assess the metabolic activity and virulence of particular species when grown together in multi-species biofilm. (am-online.org)
  • The knowledge of specific traits of multi-species biofilms may contribute to better understanding of the etiology of infectious diseases, increase the effciency of energy production in microbial fuel cells and lower the cost of biofouling prevention. (am-online.org)
  • 2. Research methods in the multi-species biofilm studies. (am-online.org)
  • 3. Dental plaque multi-species biofilm. (am-online.org)
  • 4. Multi-species biofilms in water. (am-online.org)
  • 5. Multi-species biofilms in industrial environment. (am-online.org)
  • A model multi-species sub-gingival community containing Fusobacterium nucleatum, Streptococcus constellatus , Parvimonas micra with wild type or isogenic mutants of P. gingivalis was then created and growth and proteolytic activity in serum assessed as above. (biomedcentral.com)
  • In the multi-species consortium, growth was facilitated by the wild-type and Rgp-expressing strains of P. gingivalis, suggesting that Rgp is involved in delivery of nutrients to the whole community through degradation of complex protein substrates in serum. (biomedcentral.com)
  • In doing so, they hold potential to serve as preclinical drug discovery platforms that lend well for accelerated evaluation of wound biofilm diagnostics and novel anti-biofilm therapeutics. (tigs.res.in)
  • To this end, 3-hydroxy coumarin (3HC), a compound identified from the cell-free culture supernatant of the marine bacterium Brevundimonas abyssalis, was found to exhibit anti-biofilm and anti-hyphal activity against both reference and clinical isolates of C. albicans. (solamonrenewableenergy.com)
  • The presence of these varied factors is likely to affect biofilm features such as formation and composition, and influence response to treatments. (tigs.res.in)
  • For example, resident bacterial communities establish either cooperative (beneficial) or competitive (harmful) interactions, which affect biofilm succession, biomass, and resistance to stress. (solamonrenewableenergy.com)
  • A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
  • Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
  • The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
  • Biofilm consists of microorganisms encased within a self-produced matrix of exopolysaccharides and exoproteins that strongly adheres to interfaces and resists dislodgement. (synergisticseurope.com)
  • 3] Biofilms consist of microorganisms encased within a self-produced matrix of exoproteins and exopolysaccharides, which strongly attach themselves to interfaces and highly resist dislodgement. (blogspot.com)
  • The gut microbiome is currently understood to represent a complex "organ" in the body affecting a multitude of body systems and implicated in a wide range of diseases. (isbscience.org)
  • The community-wide gene expression profiles obtained through metatranscriptomics allow to establish the activity of various genetic pathways within the microbiome. (isbscience.org)
  • In this work we (1) determine the shelf-life of ready-to-use microfluidics, (2) demonstrate biofilm-like colonization on fungi, (3) describe bacterial motility on fungal hyphae (fungal highway), (4) report material-dependent bacterial-fungal colonization, (5) demonstrate germination of vacuum-sealed Arabidopsis seeds in microfluidics stored for up to 2 weeks, and (6) observe bidirectional cytoplasmic streaming in fungi. (springer.com)
  • METHODS: We investigated the colonization of a biofilm consortia by CPKP in a model system simulating a sink-drain P-trap. (cdc.gov)
  • The human oral cavity is a highly complex ecosystem in which the tooth- and soft-tissue surfaces offer distinct ecological niches for microbial colonization. (biomedcentral.com)
  • In health, microbial communities exist in a homeostatic balance with the host that contributes to ecosystem stability and provides resistance to colonization by exogenous pathogens. (biomedcentral.com)
  • [2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
  • This can contribute to biofilm dispersal, and provides a mechanism for the release of extracellular DNA (eDNA) within biofilms. (southampton.ac.uk)
  • are selected for their ability to lyse the extracellular polymers commonly found in biofilm as well as degrade bacterial and yeast cell wall structures. (synergisticseurope.com)
  • We develop and apply single-cell genomic tools for unbiased, high-throughput expression profiling of bacterial consortia such as the animal-associated microbiota and single- and polymicrobial biofilms. (isbscience.org)
  • Centers for Disease Control (CDC) biofilm reactors (CBRs) were inoculated with microbial consortia originally recovered from 2 P-traps collected from separate patient rooms (designated rooms A and B) in a hospital. (cdc.gov)
  • These complex interactions may benefit or harm the interacting bacterial groups and aid the bacterial consortia in maintaining ecological equilibrium. (solamonrenewableenergy.com)
  • Microbial forms living in biofilms are morphologically and functionally distinct from those of free-floating (planktonic) forms of the same species. (jmb.or.kr)
  • The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
  • Metabolic activity of the bacterial biofilm communities have altered as compared to the planktonic one such as increased rates of EPS production, activation or inhibition of particular genes associated with biofilm formation and decreased growth rate [ 39 ]. (biomedcentral.com)
  • Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
  • The prime factor that is conducive to the pathogenesis of C. albicans is its ability to form biofilm on both biotic and abiotic surfaces, followed by other major virulence traits, such as yeast-to-hyphal transitions, filamentous morphology, wrinkle morphology, and secretion of proteolytic and lipolytic enzymes5. (solamonrenewableenergy.com)
  • The ability of E. meningoseptica isolates to adhere to abiotic surfaces and form biofilm structures may result from the hydrophilic cell surface and multiple adhesins located around the cell. (biomedcentral.com)
  • The Bray-Curtis index revealed that bacterial, fungal, and arbuscular mycorrhizal fungi communities were significantly different between the two farming systems. (bvsalud.org)
  • This is important given that biofilms are nearly always found as mixed-species communities, consisting of diverse bacterial and fungal species existing in close proximity with each other. (tigs.res.in)
  • Treatment of established biofilm with NEBB resulted in biofilm-disruption, involving significant reductions in biofilm mass and metabolic activity for Candida and both Staphylococcus species. (jmb.or.kr)
  • In addition, multiple species frequently co-occur and interact in a polymicrobial biofilm. (isbscience.org)
  • We aim to create single-cell gene expression maps of model single- and multiple-species biofilms and their responses to external stimuli by a combination of single-cell RNA sequencing and single-cell time-lapse imaging. (isbscience.org)
  • This cell-to-cell signaling or quorum sensing enables communication between diverse microbial species, mediating a range of processes and behaviors. (tigs.res.in)
  • All organisms, even single cells capable of surviving autonomously, have evolved to exist as part of a community of multiple interacting individuals and species. (ncbs.res.in)
  • Growth pattern of Mycobacterium species are different such as M. chelonae and M. fortuitum form biofilm as vertical and entire surface growth, respectively [ 93 ]. (biomedcentral.com)
  • Together in the subgingival consortium however, all the included species were able to grow in the serum environment and the community adopted a proteolytic phenotype. (biomedcentral.com)
  • Dysbiotic subgingival communities are characterized by a relative increase in abundance of anaerobic, Gram-negative bacterial species that can exploit protein-rich GCF as a nutrient source, promoting development of a proteolytic community phenotype. (biomedcentral.com)
  • My research group aims to understand how biofilms develop and disperse and how they respond to environmental stresses including therapeutic compounds. (southampton.ac.uk)
  • My research group aims to understand biofilm infection microenvironments, with a focus on studying wound biofilms under host-relevant conditions. (tigs.res.in)
  • Biofilm cultures were treated with NEBB containing enzymes targeted at lipids, proteins, and sugars, also containing the mucolytic compound N-acetyl cysteine, along with antimicrobial extracts from cranberry, berberine, rosemary, and peppermint. (jmb.or.kr)
  • Biofilms are notorious for their resistance to environmental stresses, including antimicrobial compounds. (southampton.ac.uk)
  • Treatment of biofilms with nitric oxide also reduced their resistance to antimicrobial compounds. (southampton.ac.uk)
  • Notably, clinical biofilms are notoriously tolerant to antimicrobial treatments, often prompting long-term antibiotic usage with uncertain reasons for failure. (tigs.res.in)
  • To study this, we employed 16S, ITS, and 18S DNA sequencing to uncover how microbial interactions were affected by conventional and organic farming systems on maize crops. (bvsalud.org)
  • Here we investigate the contribution of interactions between members of a sub-gingival community to survival and development of virulence in a serum environment-modelling that in the subgingival pocket. (biomedcentral.com)
  • The reason this discovery is groundbreaking is because previously, the formation of biofilms was a mystery, yet they are so prevalent in biology. (ucsd.edu)
  • An increase in bacterial mutation rate can promote biofilm formation. (southampton.ac.uk)
  • Recognizing this, there has been an increased interest in understanding biofilms, particularly their formation and persistence under human conditions [1]. (tigs.res.in)
  • These laboratory systems incorporate select host components, and thereby enable human-relevant insights into the formation and features of wound biofilms. (tigs.res.in)
  • Current projects include: waves of growth and death in yeast populations, the role of non-coding RNA regulation in bacterial biofilm formation, the lysis-lysogeny decision in temperate phage. (ncbs.res.in)
  • InterFase Plus includes EDTA which binds the metals needed for biofilm formation. (synergisticseurope.com)
  • As an adjunct, it is best combined with use of a high-potency, broad-spectrum, multispecies probiotic formulation and a prebiotic to encourage the formation of healthy intestinal microbial biofilm communities. (synergisticseurope.com)
  • However, biofilm formation by C. albicans has been found to gain genetic resistance against most of the currently used antifungal drugs. (solamonrenewableenergy.com)
  • Quorum sensing plays an important role in regulating the biofilm formation. (biomedcentral.com)
  • iii) colony formation and biofilm maturation. (biomedcentral.com)
  • Formation of biofilms in mycobacteria can be defined as similar as other biofilms communities. (biomedcentral.com)
  • Panels of studies have reported the role of various molecules in the biofilm formation and maintenance of its composition. (biomedcentral.com)
  • Glycopeptidolipids are indispensable for initial surface attachment during biofilm formation in Mycobacterium smegmatis [ 111 ]. (biomedcentral.com)
  • E. meningoseptica isolates CH2B and NCTC 10016 T demonstrated stronger biofilm formation in nutrient-rich medium compared to nutrient-poor medium at both 21 and 37°C, respectively. (biomedcentral.com)
  • This tolerance often links biofilms with persistent and chronic infection, and provides ideal conditions for the acquisition or evolution of AMR. (southampton.ac.uk)
  • They are the most abundant and ubiquitous organisms on Earth, playing important roles in microbial physiology, population dynamics, evolution, and therapeutics ( 1 ). (asm.org)
  • These biofilms can be also found in the industrial water distribution systems, on surfaces of the metalworking fluids tanks, in food production plants, etc. (am-online.org)
  • So far, a large component of biofilm research has used laboratory systems, such as polystyrene surfaces and protein broths, in an attempt to study biofilms and investigate treatment effects. (tigs.res.in)
  • The objective of this study was to characterize the microbial communities of disinfected, non-critical healthcare surfaces using next generation sequencing technology. (cdc.gov)
  • In this investigation, we obtained potent biofilm-forming bacterial isolates from the early-stage biofilm formed on three different artificial surfaces (stainless steel, high-density polyethylene, and titanium) immersed in the intake area of a coastal located power plant22. (solamonrenewableenergy.com)
  • Further work has shown the importance of this phage in the P. aeruginosa biofilm lifecycle and virulence. (southampton.ac.uk)
  • Intriguingly, quantitative PCR analysis of 3HC-treated C. albicans biofilm revealed significant downregulation of virulence genes (hst7, ume6, efg1, cph1, ras1, als1) associated with adhesion and morphogenesis. (solamonrenewableenergy.com)
  • Compounds originating from naturally competitive environments, such as marine biofilms, are more likely to possess greater potential to inhibit the virulence of other organisms. (solamonrenewableenergy.com)
  • Community analysis was conducted to determine the bacterial composition and compare the detection of target pathogens by culture from 94 Contact Precaution rooms. (cdc.gov)
  • Community composition over time was monitored using culture techniques and qPCR. (biomedcentral.com)
  • The results suggest that targeted nutraceutical support may help disrupt biofilm communities, offering new facets for integrative combinational treatment strategies. (jmb.or.kr)
  • It is a unique enzyme formulation with EDTA that is especially designed to disrupt the biofilm matrix that embeds gastrointestinal organisms. (synergisticseurope.com)
  • InterFase® is a unique enzyme formulation especially designed to disrupt gastrointestinal bacterial and yeast biofilm communities. (synergisticseurope.com)
  • In natural environmental conditions, marine biofilms consist of multispecies bacterial communities in close proximity, with large metabolite and protein exchange, as well as coordinated lifestyles. (solamonrenewableenergy.com)
  • Further, biofilms possess distinct nutrient acquisition and protection mechanisms, contributing to their extended survival and persistence. (tigs.res.in)
  • [10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
  • Here, we examined the relative abundance of soil microbial keystone taxa and their functional potentials, as well as their influence on soil carbon storage with increased precipitation across alpine grasslands on the QTP, China. (bvsalud.org)
  • Our study offers valuable insights into the intricate correlation between precipitation-induced alterations in soil microbial keystone taxa and SOC storage, highlighting a rough categorization is difficult to distinguish the hidden threats and the importance of incorporating functional potentials in SOC storage prediction models in response to changing climate. (bvsalud.org)
  • For P. aeruginosa , low doses of NEBB significantly reduced biofilm mass and metabolic activity while higher doses of NEBB increased biofilm mass and metabolic activity. (jmb.or.kr)
  • The strains were allowed to form biofilm in vitro. (jmb.or.kr)
  • The Microbiology Society is working with the scientific community to engage with policy-makers on the issue of Brexit. (microbiologysociety.org)
  • We are a not-for-profit publisher and we support and invest in the microbiology community, to the benefit of everyone. (microbiologysociety.org)
  • Join the Microbiology Society and become part of the largest microbiology community in Europe. (microbiologysociety.org)
  • To develop effective therapies, we must understand how the behavior of a microbial community emerges from the diverse contributions of its individual members. (isbscience.org)
  • Through long-range electric signaling, a biofilm can alter the behavior of a cell outside of its community, 'convincing' it to join the biofilm. (ucsd.edu)
  • The post-treatment biofilm mass was evaluated by crystal-violet staining, and metabolic activity was measured using the MTT assay. (jmb.or.kr)
  • Average biofilm mass and metabolic activity for NEBB-treated biofilms were compared to the average of untreated control cultures. (jmb.or.kr)
  • For B. burgdorferi , we observed reduced biofilm mass, but the remaining residual biofilm showed a mild increase in metabolic activity, suggesting a shift from metabolically quiescent, treatment-resistant persister forms of B. burgdorferi to a more active form, potentially more recognizable by the host immune system. (jmb.or.kr)
  • Early childhood caries (ECC) - dental caries (cavities) occurring in primary teeth up to age 6-years - is a prevalent childhood oral disease with a microbial etiology. (researchsquare.com)
  • Bacterial communities commonly exist as of biofilms, such as the plaque that grows on your teeth or the slime you can find in a clogged sink. (ucsd.edu)
  • Biofilm, also called slime, in the flow boundary skin layer of rapid swimmers (e.g. barracuda) can effectively subdue turbulence and thus prevent energy loss. (blogspot.com)
  • E. meningoseptica isolate CH2B and strain NCTC 10016 T displayed a strong biofilm-forming phenotype which may play a role in its potential pathogenicity in both clinical and aquaculture environments. (biomedcentral.com)
  • In more complex settings, such as biofilms, different sub-populations may take on different tasks that are all required for proper biofilm functioning, not unlike tissues in multicellular organisms. (ncbs.res.in)
  • In clinical conditions, biofilms exist in close proximity with a range of host components, such as cells, tissues, and body fluids. (tigs.res.in)
  • Dysbiosis in subgingival microbial communities, resulting from increased inflammatory transudate from the gingival tissues, is an important factor in initiation and development of periodontitis. (biomedcentral.com)
  • When the adjacent gingival soft-tissues are inflamed, the flow of serum-like transudate (gingival crevicular fluid, GCF) into the pocket increases and under the influence of this environmental perturbation, homeostasis in the subgingival microbial community can be disturbed giving rise to dysbiosis that predisposes the site to disease [ 3 ]. (biomedcentral.com)
  • Biofilms are spatially structured bacterial communities that contribute to chronic bacterial diseases. (isbscience.org)
  • intercepting the microbial text message could lead to the cure to hundreds of bacterial diseases. (ucsd.edu)
  • Not unlike individual cells in the human body, they may assume specialized roles for the benefit of the whole community. (isbscience.org)
  • Many cells within the biofilm are shielded from host defenses and drugs. (isbscience.org)
  • Biofilm architecture of isolate CH2B alone as well as in co-culture with Listeria monocytogenes was investigated using flow cells and microscopy. (biomedcentral.com)
  • The enzyme blend in InterFase Plus was developed using in vitro testing of antibiofilm activity and was found to have significant antibiofilm activity resulting in meaningful degradation of undesirable biofilm communities. (synergisticseurope.com)
  • CAV1016 showed no significant difference in quantity or persistence based on biofilm age (7 days vs 28 days) or substratum type (SS vs PVC). (cdc.gov)
  • CONCLUSIONS: These results suggest that CPKP persistence in P-trap biofilms may be strain specific or may be related to the type of P-trap material or age of the biofilm. (cdc.gov)
  • Their ability to exist as biofilm structures has been alluded to but not extensively investigated. (biomedcentral.com)
  • Streptococcus mutans was previously considered a primary cause, but recent research promotes the ecologic hypothesis, in which a dysbiosis in the oral microbial community leads to caries. (researchsquare.com)
  • Dirichlet multinomial community state typing and co-occurrence network analysis identified similar robust and replicable groups of co-occurring taxa. (researchsquare.com)
  • Intragenera competitions and intergenera cooperation between oral taxa may shape the emergence of these communities, providing points for preventive interventions. (researchsquare.com)
  • RESULTS: Microbial communities in model systems (designated CBR-A or CBR-B) were less diverse than communities in respective P-traps A and B, and they were primarily composed of and Proteobacteria, as determined using 16S rRNA community analysis. (cdc.gov)
  • Cell lysis as a feature of biofilm development. (southampton.ac.uk)
  • In the last few years, considerable emphasis has been given in both microbial phylogeny and microbial ecology to the development of methods for studying the larger and more informative rRNAs. (printerresource.com)
  • Following biofilm development CBRs were inoculated with either K. pneumoniae ST45 (ie, strain CAV1016) or K. pneumoniae ST258 KPC+ (ie, strain 258), and samples were collected over 21 days. (cdc.gov)
  • The anionic properties of EPS sugars in the wall of biofilms attract the divalent cations such as Ca+2, Mg+2, and more importantly the toxic metal cations, which have been shown to cross-link with the polymer strands and provide greater binding force and structural strength in a developed biofilm. (blogspot.com)
  • In this incident density sampled case control study of 189 children followed from 2-months to 5-years, we use the salivary bacteriome to 1) prospectively test the ecological hypothesis of ECC in salivary bacteriome communities and 2) identify co-occurring salivary bacterial communities predicting future ECC. (researchsquare.com)
  • Bacterial communities which assemble before 12-months of age can promote or inhibit an ecological succession to S. mutans dominance and cariogenesis. (researchsquare.com)
  • Microbial biofilms form on liquid/solid interfaces in nature, such as rocks and clay particles and decaying plant materials. (jmb.or.kr)
  • A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)