• Chromatin is structured on a number of different levels, by the covalent modification of nucleosomes, the arrangement of nucleosomes into chromatin fibres and the arrangement of chromatin fibres into higher order structures within the interphase nucleus. (bl.uk)
  • In asf1 KD embryos, HIRA localizes to the male nucleus but is only capable of limited and insufficient chromatin assembly. (ens-lyon.fr)
  • Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase. (yeastgenome.org)
  • However, the functional links between intracellular signaling-dependent gene regulation and epigenetic regulation by chromatin-modifying proteins within the nucleus are largely unknown. (elsevierpure.com)
  • Each human cell contains the equivalent of two meters of DNA packed in a small, micrometre-sized nucleus in the form of chromatin. (imperial.ac.uk)
  • The first genome-wide examination of the chromatin landscape at the periphery of the plant cell nucleus reveals substantial enrichment of heterochromatin and Polycomb -based repressive chromatin. (biomedcentral.com)
  • The organization of chromatin in the cell nucleus is currently mainly studied with the Hi-C technique, which uses sequencing to quantify contacts between different DNA regions. (pasteur.fr)
  • Cd treatment reduced the ubiquitination of Nrf2, increased the nuclear accumulation of Nrf2, and disrupted the Nrf2/Keap1 complex in the nucleus. (cdc.gov)
  • The first suggests that disruption of the inner nuclear membrane and the nuclear lamina causes disorganization of nuclear chromatin and gene expression, while the second proposes that the mechanical strength of the cell nucleus is disrupted when the nuclear lamina is weakened leading to structural and signaling defects in mechanically stressed tissue such as muscle and heart. (medscape.com)
  • The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. (johnshopkins.edu)
  • Both sth1 and sfh1 mutants exhibit altered centromeric and centromere-proximal chromatin structure and increased missegregation of authentic chromosomes. (johnshopkins.edu)
  • The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. (nih.gov)
  • This chromatin remodelling activity is associated with regulation of gene expression in heterochromatin regions of plant and mammal chromosomes and with the cellular response to DNA damage. (imperial.ac.uk)
  • Scientists from the Institut Pasteur and the CNRS have developed a new method for quantifying chromatin loops and observing chromosomes at high resolution. (pasteur.fr)
  • These chromatin loops help structure chromosomes and play an important role in basic genome functions such as gene expression. (pasteur.fr)
  • The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
  • We employed site-specific crosslinking to identify proteins that directly bind the acidic patch in Saccharomyces cerevisiae and demonstrated crosslinking of histone H2A to Paf1 complex subunit Rtf1 and FACT subunit Spt16. (biorxiv.org)
  • Our results provide new information on the chromatin engagement of two central players in transcription elongation and emphasize the importance of the nucleosome core as a hub for proteins that regulate chromatin during transcription. (biorxiv.org)
  • Epigenetic regulation refers to the multiple chemical modifications of DNA or DNA-associated proteins that alter chromatin structure and DNA accessibility in a heritable manner, without changing DNA sequence. (frontiersin.org)
  • ChIP utilizes antibodies that selectively recognize and bind proteins, including histones, histone modifications, transcription factors, and cofactors, to provide information about chromatin states and gene transcription. (cellsignal.com)
  • ChIP can be used to answer a multitude of scientific questions involving the interaction of proteins and chromatin. (cellsignal.com)
  • In N-ChIP, no fixing agent is used to crosslink proteins to the chromatin. (cellsignal.com)
  • In metazoans, a complex meshwork of intermediate filament proteins constitutes the so-called lamina at the inner nuclear membrane to which chromatin regions and associated factors are anchored [ 2 ]. (biomedcentral.com)
  • This complex includes nuclear membrane integral and associated proteins including emerin, lamin A/C, SUN1, SUN2, nesprin-1, and nesprin-2 that are proposed to form a mechanical link between the nucleoskeleton and cytoskeleton. (medscape.com)
  • Chromatin remodeling complexes are required for many distinct nuclear processes during replication and transcriptional regulation. (nih.gov)
  • In addition, work on the R2TP complex (which contains the INO80 APases Ruvb1/Ruvb2, and subunits Tah1 and Phi1) has revealed the recruitment mechanism for the molecular chaperone, Hsp90, and the telomere length regulation protein, Tel2. (figshare.com)
  • Here, we describe novel epigenetic regulation by MAPK cascades that modulate formation of an ATP-dependent chromatin remodeling complex, WINAC (WSTF Including Nucleosome Assembly Complex), an SWI/SNF-type complex containing Williams syndrome transcription factor (WSTF). (elsevierpure.com)
  • WINAC mediates a previously unknown MAPK-dependent step in epigenetic regulation, and this MAPK-dependent switching mechanism between the two functionally distinct WSTF-containing complexes might underlie the diverse functions of WSTF in various nuclear events. (elsevierpure.com)
  • Cadmium coordinates up-regulation of cytoprotective genes and protection agains metal toxicity by inhibiting the ubiquitination of Nrf2 and disrupting the Nrf2/Keap1 complex. (cdc.gov)
  • The method uses formaldehyde fixation to stabilize protein complexes. (nature.com)
  • Functional organization of the yeast proteome by systematic analysis of protein complexes. (nature.com)
  • Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. (nature.com)
  • Global landscape of protein complexes in the yeast Saccharomyces cerevisiae . (nature.com)
  • Babu, M., Krogan, N.J., Awrey, D.E., Emili, A. & Greenblatt, J.F. Systematic characterization of the protein interaction network and protein complexes in Saccharomyces cerevisiae using tandem affinity purification and mass spectrometry. (nature.com)
  • Together, the R2TP complex, Hsp90 and Tel2 promote the stabilisation and maturation of multi-protein complexes. (figshare.com)
  • The super-structure of chromatin is regulated by ring-shaped, ATP-dependent molecular motors belonging to the SMC family of protein complexes. (imperial.ac.uk)
  • Histone modifications and chromatin-associated protein complexes are crucially involved in the control of gene expression, supervising cell fate decisions and differentiation. (researchgate.net)
  • We have a particular focus on how different protein complexes package repetitive DNA into chromatin, and how chromatin influences transcriptional dynamics. (lu.se)
  • The formation of a diploid zygote begins with the unique, genome-wide RI assembly of paternal chromatin following sperm protamine eviction. (ens-lyon.fr)
  • It is optimized for ChIP reactions with chromatin from 10 6 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 10 8 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications. (sigmaaldrich.com)
  • However, the genome-wide chromatin accessibility has been only reported in limited tissue types in pigs. (biomedcentral.com)
  • Here we report a genome-wide landscape of chromatin accessibility of 20 tissues in two female pigs at ages of 6 months using ATAC-seq, and identified 557,273 merged peaks, which greatly expanded the pig regulatory element repository. (biomedcentral.com)
  • Whether SWI/SNF chromatin remodeling complexes play roles in embryonic stem (ES) cells remains unknown. (elsevierpure.com)
  • The work presented here underscores the importance of SWI/SNF chromatin remodeling complexes in pluripotent stem cells. (elsevierpure.com)
  • WSTF, a specific component of two chromatin remodeling complexes (SWI/SNF-type WINAC and ISWI-type WICH), was phosphorylated by the stimulation of MAPK cascades in vitro and in vivo. (elsevierpure.com)
  • The proposed study will be the first to describe the chromatin accessibility landscape of liposarcoma and to elucidate how differences in the noncoding genomes and complex chromosomal rearrangements between WDLPS and DDLPS histologies affect chromatin "openness," downstream gene regulatory networks, and ultimately disparate biologic subtype-specific behavior and prognosis. (curesarcoma.org)
  • We developed an approach combining imaging with polymer simulations to describe the chromatin structure in individual human cells," explains Christophe Zimmer, Head of the Imaging and Modeling laboratory at the Institut Pasteur. (pasteur.fr)
  • A role for the multi-subunit BAF (mammalian SWI/SNF) complex in brain development has been postulated as its dysfunction causes several neurological diseases, including intellectual disability, schizophrenia, autism spectrum disorders, and amyotrophic lateral sclerosis. (uni-goettingen.de)
  • SWR1 complex (ySWR1 in yeast, and hSRCAP in humans) is a 1.1 MDa multi-subunit complex that utilizes ATP to replace canonical H2A histones with the Htz1 variant (H2A.Z in mammalian cells). (imperial.ac.uk)
  • A comprehensive landscape of chromatin states for multiple mammalian tissues is essential for elucidating the molecular mechanism underlying regulatory variants on complex traits. (biomedcentral.com)
  • Here I focus on the technological approaches by which we discovered that hotspots also transition from closed to open chromatin, dependent on the chromatin remodeler HELLS. (umaine.edu)
  • We show that Bonus associates with the histone methyltransferase SetDB1 and the chromatin remodeler NuRD and depletion of either component releases Bonus-induced repression. (elifesciences.org)
  • The imitation SWI (ISWI) is an ATP-dependent chromatin remodeling complex and is represented by enzymatic subunits Snf2h (Smarca5) and Snf2l (Smarca1). (nih.gov)
  • Here, we report that the loss of BAF155/BAF170 in double-conditional knockout (dcKO) mice eliminates all known BAF subunits, resulting in an overall reduction in active chromatin marks (H3K9Ac), a global increase in repressive marks (H3K27me2/3), and downregulation of gene expression. (mpg.de)
  • The INO80 complex is a large ATPase chromatin remodeller which contains 15 accessory subunits in S.cerevisiae. (figshare.com)
  • These include Phosphatidylinositol 3-kinase-related kinases (PIKKs, a family of kinases involved i Serine and Threonine phosphorylation), subunits of the INO80 complex and subunits of the SWR1 chromatin remodelling complex (a partner comlex to INO80 that incorporates H2A.Z into chromatin). (figshare.com)
  • Subunit exchange of CHDs, the core ATPase subunits of the NuRD complex, is required for distinct aspects of cortical development. (figshare.com)
  • Chromatin accessibility complex 1 is a protein that in humans is encoded by the CHRAC1 gene. (wikipedia.org)
  • We investigated the presence of transposable elements (TEs) in open chromatin regions across all tissues, these included identifications of porcine endogenous retroviruses (PERVs) exhibiting high accessibility in liver and homology of porcine specific virus sequences to universally accessible transposable elements. (biomedcentral.com)
  • Over the past decades, several chromatin accessibility profiling methods including DNase I hypersensitive site sequencing (DNase-seq), micrococcal nuclease sequencing (MNase-seq) and assay for transposase-accessible chromatin using sequencing (ATAC-seq) have been developed. (biomedcentral.com)
  • Another hallmark of the gene regulatory landscape is chromatin accessibility, which is regulated by epigenetic pathways and can be dysregulated as a result of both chromosomal rearrangements and mutations in the noncoding genome and which have been unstudied in liposarcoma. (curesarcoma.org)
  • We hypothesize that chromatin accessibility differ as a result of changes occurring in the noncoding genomes and complex chromosomal rearrangements in WDLPS and DDLPS and contribute to dysregulation of downstream gene regulatory networks. (curesarcoma.org)
  • The aims of the proposed study are to 1) characterize and determine differences in the chromatin accessibility landscapes of treatment naïve WDLPS and DDLPS and 2) understand the functional consequences of differences in the noncoding genomes and complex chromosomal rearrangements on chromatin accessibility and downstream gene regulatory networks in treatment-naive WDLPS and DDLPS. (curesarcoma.org)
  • Recent studies in S. cerevisiae have identified the subunit Ies6 as a critical component of the INO80 complex. (figshare.com)
  • In this study, we have determined the crystal structure of one subunit in an ATP-dependent chromatin remodeling factor and suggested the overall shape combined with a lot of physicochemical, biochemical and molecular biological methods. (nii.ac.jp)
  • Overexpression of the Arabidopsis Anaphase Promoting Complex subunit CDC27a increases growth rate and organ size. (helsinki.fi)
  • Here we identified MBTD1 as a stable subunit of the complex, and we reveal that, via a histone reader domain for H4K20me1/2, MBTD1 allows TIP60 to associate with specific gene promoters and to promote the repair of DNA double-strand breaks by homologous recombination. (houstonmethodist.org)
  • These complex multi-subunit machines carry out multiple tasks on nucleosomes including chemical modifications, histone exchange and sliding them on DNA in what appears to be a highly coordinated process. (imperial.ac.uk)
  • In a recent study, Bi and colleagues identified genomic regions that are associated with the Arabidopsis NUCLEOPORIN1 (NUP1) protein, an inner subunit of the nuclear pore complex (NPC) [ 5 ] responsible for mRNA export that protrudes out into the lamina-like matrix (Fig. 1 ). (biomedcentral.com)
  • Although the clinical implications of chromosomal aberrations in liposarcomas are incompletely understood, in other malignancies complex chromosomal rearrangements have been shown to alter chromatin folding, architecture, and organization which in turn impact functional genomic processes and the tumor regulatory landscape through altered near- and long-range interactions. (curesarcoma.org)
  • Altogether, these results uncover an intricate mechanism orchestrated by the TIP60 complex to regulate 53BP1-dependent repair through competitive bivalent binding and modification of chromatin. (houstonmethodist.org)
  • Using camalexin biosynthesis genes as an example, we confirmed that these two modifications were co-localized to form bivalent chromatin. (biorxiv.org)
  • Our study suggests that this type of bivalent chromatin, which we name a kairostat, controls the precise timing of gene expression upon stimuli. (biorxiv.org)
  • reports suggested that targeting of H2A.Z to bivalent promoters may depend on PRC1/2 complexes and vice versa ( Creyghton et al. (researchgate.net)
  • Background: Bivalent chromatin domains consisting of the activating histone 3 lysine 4 trimethylation (H3K4me3) and repressive histone 3 lysine 27 trimethylation (H3K27me3) histone modifications are enriched at developmental genes that are repressed in embryonic stem cells but active during differentiation. (researchgate.net)
  • Bivalent promoters are defined by the presence of both activating (H3K4me3) and repressive (H3K27me3) chromatin marks. (researchgate.net)
  • Histone modifications and chromatin remodeling represent universal mechanisms by which cells adapt their transcriptional response to rapidly changing environmental conditions. (figshare.com)
  • These roles are in addition to its traditional nucleosome remodelling activities and the dispacement of H2A.Z from chromatin. (figshare.com)
  • The structure of an enzyme that is bound to a nucleosome - a protein complex around which DNA is wrapped - reveals how contacts between the two orient the enzyme so that it can modify a specific amino-acid residue. (natureasia.com)
  • The SMARCB1 C-terminal alpha-helical domain binds the nucleosome acidic patch and mediates mSWI/SNF chromatin remodeling. (princeton.edu)
  • a Artist impression representing Arabidopsis interphase nuclei and the potential links between nuclear pore complexes ( NPC ) and contrasting chromatin contexts. (biomedcentral.com)
  • Affinity purification strategies for proteomic analysis of transcription factor complexes. (nature.com)
  • The principle behind ChIP is relatively straightforward and relies on the use of an antibody to isolate, or precipitate, a certain protein, histone, transcription factor, or cofactor and its bound chromatin from a protein mixture that was extracted from cells or tissues. (cellsignal.com)
  • Epigenetics Chromatin, 11(1):19. (ens-lyon.fr)
  • The topic of my lecture is skin epigenetics or the story on how chromatin regulators orchestrate skin functions. (hstalks.com)
  • Epigenetics Chromatin. (wayne.edu)
  • In the Lab of Epigenetics and Chromatin Dynamics ( https://www.stemcellcenter.lu.se/research-groups/douse ) we are interested in how this genomic dark matter is controlled by epigenetic mechanisms during human brain development. (lu.se)
  • Nevertheless, a full understanding of their role in development and chromatin remodeling has been hindered by the absence of mutants completely lacking BAF complexes. (mpg.de)
  • The Epidermal Differentiation Complex (EDC) is a co-ordinately regulated locus that is evolutionarily conserved within mammals. (bl.uk)
  • Despite this disease-related interest, there is little known about regulatory mechanisms exerted by BAF complex in the proliferative control of the oligodendrocytes (OL) progenitors, differentiation and migration of the GE-derived neuronal progenitors. (uni-goettingen.de)
  • Herein, we show that BAF complex acts as cell cycle regulator, controlling the dynamic balance between proliferation and differentiation of OL progenitors. (uni-goettingen.de)
  • Remarkably, the deletion of BAF complex impaired the proliferative capacity of the OPCs, and consequently diminished the ability of OLs differentiation and maturation. (uni-goettingen.de)
  • Our data revealed that conditional absence of BAF complex in the MGE progenitors lead to severe lessening of the MGE-derived INs, along with impairment of neuronal differentiation and migration. (uni-goettingen.de)
  • Overall, these findings emphasize that BAF complex is crucial for proper proliferative division and enhance of OPCs and IN differentiation during murine brain development. (uni-goettingen.de)
  • Importantly, BAF complexes are indispensable for forebrain development, including proliferation, differentiation, and cell survival of neural progenitor cells. (mpg.de)
  • Here we show that SWI/SNF complexes are present in mouse ES cells, and their composition is dynamically regulated upon induction of ES cell differentiation. (elsevierpure.com)
  • The altered expression of SWI/SNF components hinted that these complexes could play roles in ES cell maintenance or differentiation. (elsevierpure.com)
  • While structural and biochemical studies have shown that the acidic patch controls chromatin factor binding and activity, few studies have elucidated its functions in vivo . (biorxiv.org)
  • BAF (Brg/Brm-associated factors) complexes play important roles in development and are linked to chromatin plasticity at selected genomic loci. (mpg.de)
  • Our knowledge of chromatin organization and function in plant systems is rapidly expanding, and the roles of different nuclear peripheral components in structuring chromatin are also beginning to emerge [ 1 ]. (biomedcentral.com)
  • A new study conducted by scientists at the International Agency for Research on Cancer (IARC), with partners from Charles University in Prague (Czechia), describes new roles of a key chromatin remodelling enzyme, Smarca5, which is often deregulated in human cancers. (who.int)
  • The new results show that Smarca5 plays crucial and complex roles in the maintenance of primary cell fitness, including protecting cells from exposure to mutagenic chemicals. (who.int)
  • These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. (wikipedia.org)
  • At DNA replication forks, ASF1 plays an important role in regulating the supply of H3.1/2 and H4 to the CAF-1 chromatin assembly complex. (ens-lyon.fr)
  • Their enrichment in enhancer-like chromatin signatures, interactions with nearby trait-relevant protein-coding loci, and preferential location at topologically associated domain (TAD) boundaries provide evidence that TR-lincRNAs likely regulate proximal trait-relevant gene expression in cis by modulating local chromosomal architecture. (nih.gov)
  • Our data provides a novel multi-tissues accessible chromatin landscape that serve as an important resource for interpreting regulatory sequences in tissue-specific and conserved biological functions, as well as regulatory variants of loci associated with complex traits in pigs. (biomedcentral.com)
  • We describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. (cdc.gov)
  • However, structural bases for enzyme mechanisms through chromatin remodeling are not known. (nii.ac.jp)
  • Using biochemical analysis of a WSTFmutant (WSTF-S158A) stably expressing cell line, the phosphorylation of this residue (Ser-158) was found to be essential for maintaining the association between WSTF and core BAF complex components, thereby maintaining the ATPase activity of WINAC. (elsevierpure.com)
  • ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization. (ens-lyon.fr)
  • In collaboration with Prof Dale Wigley (Imperial College London) , we are investigating the molecular mechanism of ATP-dependent replacement of the canonical two H2A histones with the Htz1 variant by the ySWR1 complex. (imperial.ac.uk)
  • SUMOylation influences Bonus's subnuclear localization, its association with chromatin and interaction with SetDB1. (elifesciences.org)
  • Recent efforts have shown that structural variations (SVs) can disrupt three-dimensional genome organization and induce enhancer hijacking, yet no computational tools exist to identify such events from chromatin interaction data. (nature.com)
  • This study provides new information about basic genome organization structures, as well as a method to visualize and model the detailed structure of chromatin. (pasteur.fr)
  • Loss of BAF (mSWI/SNF) complexes causes global transcriptional and chromatin state changes in forebrain development. (mpg.de)
  • The double-membrane nuclear envelope provides a physical interface that contributes to the compartmentalization of chromosomal domains that have distinct chromatin states and activities within the nuclear space. (biomedcentral.com)
  • Buehl CJ, Goff NJ, Hardwick SW, Gellert M, Blundell TL, Yang W, Chaplin AK , Meek K. Two distinct long-range synaptic complexes promote different aspects of end processing prior to repair of DNA breaks by non-homologous end joining. (le.ac.uk)
  • Failure to reorganize chromatin at hotspots into an open conformation leads to DSBs at other open regions, such as promotors, causing a breakdown in recombination and cell death. (umaine.edu)
  • Together, our work identified Bonus as a regulator of tissue-specific gene expression and revealed the importance of SUMOylation as a regulator of complex formation in the context of transcriptional repression. (elifesciences.org)
  • The NuA4/TIP60 acetyltransferase complex is a key regulator of genome expression and stability. (houstonmethodist.org)
  • Liu M, Saha N, Gajan A, Saadat N, Gupta SV, Pile LA. A complex interplay between SAM synthetase and the epigenetic regulator SIN3 controls metabolism and transcription. (wayne.edu)
  • We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
  • Our findings reveal a molecular mechanism mediated by BAF complexes that controls the global transcriptional program and chromatin state in development. (mpg.de)
  • While some of their biological functions have been well described, the molecular mechanism by which these complexes function remains poorly understood. (imperial.ac.uk)
  • Finally, we demonstrate that NuRD complexes containing specific CHDs are recruited to regulatory elements and modulate the expression of genes essential for brain development. (figshare.com)
  • Genes responsible for the biosynthesis of these compounds are wired into a complex network. (biorxiv.org)
  • In eukaryotic cells, the three types of SMC complexes are cohesin, condensin and SMC5/6. (imperial.ac.uk)
  • Our framework can automatically resolve complex SVs, reconstruct local Hi-C maps surrounding the breakpoints, normalize copy number variation and allele effects and predict chromatin loops induced by SVs. (nature.com)
  • We have known for some years that chromatin fiber forms loops that are maintained by a molecule complex called cohesin. (pasteur.fr)
  • It is thought that the developmental program of gene expression at the locus is regulated by specific changes in chromatin structure (Williams et al. (bl.uk)
  • 2002). To investigate this, I have characterised the chromatin structure of the EDC in cultured cell lines. (bl.uk)
  • By doing so I have examined the role each level of chromatin structure plays in regulating the human EDC and, characterised the relationships between the different levels across a large co-ordinately regulated locus in the human genome. (bl.uk)
  • In order to improve and expand applications of Cas9, we recently undertook crystallographic studies to elucidate the atomic structure of the Cas9-sgRNA-target DNA tertiary complex 14 , thus enabling rational engineering of Cas9 and sgRNA. (cdc.gov)
  • This separation of tasks could optimize the rapid assembly of paternal chromatin within the gigantic volume of the egg cell.In contrast, ASF1 is surprisingly dispensable for the amplification of cleavage nuclei, although chromatin integrity is likely compromised in KD embryos. (ens-lyon.fr)
  • Instead, native chromatin is isolated from cell nuclei that are digested with a nuclease. (cellsignal.com)
  • Chromatin landscape at the inner periphery of Arabidopsis nuclei. (biomedcentral.com)
  • AML has a characteristic dependency on chromatin and transcriptional regulators, which has been discussed in many reviews. (frontiersin.org)
  • Histone 2B monoubiquitination complex integrates transcript elongation with RNA processing at circadian clock and flowering regulators. (helsinki.fi)
  • Interplay between transcriptional regulators and the SAGA chromatin modifying complex fine-tune iron homeostasis. (bvsalud.org)
  • The human fungal pathogen Candida albicans responds to iron deprivation by a global transcriptome reconfiguration known to be controlled by the transcriptional regulators Hap43 (also known as Cap2), Sef1, and the trimeric Hap2-Hap3-Hap5 complex. (bvsalud.org)
  • Thus, a regulatory network involving iron -responsive transcriptional regulators and the SAGA histone modifying complex functions as a molecular switch to fine-tune tight control of iron homeostasis gene expression in C. albicans. (bvsalud.org)
  • Two DNA molecules held by a quad-optical trap (Lumicks) are held together by a Cohesin complex, which can slide on the DNA without letting go of the DNA strands. (imperial.ac.uk)
  • The scientists used this method to analyze the influence of cohesin on the chromatin structures. (pasteur.fr)
  • Engineering complex tissues requires high-throughput, three-dimensional patterning of materials and cells. (natureasia.com)
  • It was previously suggested that TIP60-dependent acetylation of H4 regulates binding of the non-homologous end joining factor 53BP1, which engages chromatin through simultaneous binding of H4K20me2 and H2AK15ub. (houstonmethodist.org)
  • HELLS and PRDM9 form a pioneer complex that targets hotspot sequences and provides access for the DNA double-strand break (DSB) machinery. (umaine.edu)
  • These findings define a previously unknown role for the adenosine triphosphate-dependent chromatin remodeling machinery. (elsevierpure.com)
  • Thus, the trimeric HAP complex promotes FRP1 transcription by stimulating H3K9Ac and Pol II recruitment and, along with Hap43, functions as a repressor of ACO1 by maintaining a deacetylated promoter under iron -deficient conditions. (bvsalud.org)
  • Confocal time-lapse movie initially shows a single dCas9 complex (green dot) bound on-target to λ-DNA held between two beads by optical tweezers at low force (5 pN). (imperial.ac.uk)
  • Off-targets dissociate while decreasing the force back to 5 pN, and only the on-target complex remains bound. (imperial.ac.uk)