Complete genomic sequencing short amplicons showed a deletion. Medical search. Frequent questions

To ease future molecular studies, this study compares first- (partial sequencing via Sanger), second- (Illumina), and third-generation (MinION Nanopore) sequencing platforms for USUV. (frontiersin.org)
The high-throughput - next generation sequencing (HT-NGS) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the Sanger method. (springer.com)
Residual amounts of amplified genomic DNA are minimized and do not interfere with mitochondrial-specific downstream methods such as qPCR or direct Sanger sequencing. (qiagen.com)
In addition, samples enriched by hybridization capture had fewer exons with mean coverage less than 10, reducing the need for followup Sanger sequencing. (biomedcentral.com)
However, comprehensive sequencing of the many genes involved is often impractical with traditional Sanger methods. (biomedcentral.com)
While NGS offers a promising alternative to Sanger sequencing, the clinical utility of NGS methods depends on the ability to accurately isolate or amplify the genomic regions of interest. (biomedcentral.com)

CRISPR (/ˈkrɪspər/) (an acronym for clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. (wikipedia.org)
CRISPR is found in approximately 50% of sequenced bacterial genomes and nearly 90% of sequenced archaea. (wikipedia.org)
The 'VirION' (Viral, long-read metagenomics via MinION sequencing) approach was first validated using mock communities where it was found to be as relatively quantitative as short-read methods and provided significant improvements in recovery of viral genomes. (peerj.com)
Continual advancements in genomic and computational technologies have allowed researchers to construct precise pictures of individual genomes and identify variations that distinguish one genome from another. (seqanswers.com)
With the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cost of $1,000 each is seemed to be realistically feasible in the near future. (springer.com)
During this period, tremendous success has been achieved in the fields of decoding of human genome, technological advancement of new era of human genome applications, toward personalized genomes and discovery of rare variants, leveraging genome sequencing to impact on cancer researches and mammalian evolution and population structure. (springer.com)
Using shotgun metagenomics from skin swabs of healthy individuals and patients with AD, we found 13,586 potential viral contiguous DNA sequences, which could be combined into 164 putative viral genomes including 133 putative phages. (microbial-ecology.net)

To overcome this limitation, we performed cDNA-based high-throughput amplicon sequencing (HTAS) in 250 FLT3- ITD positive AML patients, treated on German AML Cooperative Group (AMLCG) trials. (oncotarget.com)
We confirmed host phylogeny using the sequencing method and analyzed the influence of host phylogeny and bioclimatic zone parameters on the rodent gut microbiome using high-throughput amplicon sequencing of 16S rRNA gene fragments. (microbial-ecology.net)

7- 9 All reported mutations are single nucleotide alterations leading to splice site, missense, nonsense, or frameshift mutations, or intra-exonic deletions and insertions of up to four nucleotides, which have been detected through exonic PCR amplifications and sequencing. (bmj.com)
BLAST analysis indicated that nucleotide sequences of PCV2 amplified fragments were the most similar to the Austrian isolate of PCV2. (uvlf.sk)
Short-read viral metagenomic studies have provided clues to viral function and first estimates of global viral gene abundance and distribution, but their assemblies are confounded by populations with high levels of strain evenness and nucleotide diversity (microdiversity), limiting assembly of some of the most abundant viruses on Earth. (peerj.com)
We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
The considerable number of HBV isolates with rather divergent nucleotide sequences and the partially double-stranded characteristic of HBV impose the need for extreme care in the choice of primers for both full length and fragment amplification. (biomedcentral.com)
2012 ). Due to the high linear energy transfer (LET) properties of heavy ion beams, single nucleotide variations (SNVs) and insertions/deletions (InDels) and structural variations (SVs) can be induced at higher frequencies (Zheng et al. (springeropen.com)

Surprisingly, germline variants in SAMD9 or SAMD9L were present in 17% of primary MDS patients, and these variants were routinely lost in the tumor cells by chromosomal deletions (e.g., monosomy 7) or copy number neutral loss of heterozygosity (CN-LOH). (nature.com)
In contrast, other studies using targeted sequencing of children with idiopathic bone marrow failure or MDS found pathogenic variants in only approximately 10% of patients 16 , suggesting the need for more comprehensive sequencing. (nature.com)
When you sequence, phasing refers to the process of identifying those unique variants on each DNA sequencing read and then separating (phasing) those reads into their respective parental haplotypes. (seqanswers.com)
Researchers are able to directly phase long reads when a single sequence read spans the interval between two genomic variants. (seqanswers.com)
After the appropriate sequencing reads are generated, the next steps are to assemble the reads, detect the variants, and phase the haplotypes. (seqanswers.com)
whole genome sequencing identified new grain length gene allelic variants GS3-G1, GS3-G2 and GS3-G3. (springeropen.com)
Amplicon sequence variants (ASVs) were identified that were unique to the Sudanian bioclimatic zone. (microbial-ecology.net)

Our data show that, in contrast to adult MDS, Ras/MAPK pathway mutations are common in pediatric MDS (45% of primary cohort), while mutations in RNA splicing genes are rare (2% of primary cohort). (nature.com)
We show that Ras/MAPK pathway mutations are common in pediatric primary MDS (45%) while mutations in RNA splicing genes are rare (2%), and that germline SAMD9/SAMD9L mutations are present in 17% of primary MDS patients. (nature.com)
The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. (nature.com)
The genome-edited events of plantain Gonja Manjaya were generated with mutations in the targeted sites of integrated eBSV sequences in the host genome. (nature.com)
Penetrance of complex II mutations shows peculiar characteristics. (bmj.com)
Mutations in SDHD cause PGL1 only if the transmission occurs paternally, whereas maternal transmission does not cause disease, 13 suggesting operation of genomic imprinting on SDHD . (bmj.com)
Because the molecular basis of the parent of origin effects in PGL1 is unknown, it is unclear whether transmissions of mutations in SDHC , the protein product of which couples with that of SDHD and forms the membrane spanning domain of mitochondrial complex II, also shows any parent of origin effects. (bmj.com)
Mutation analysis of the TBCE gene of this syndrome was shown to be due to Polymerase chain reaction/single-strand mutations in the TBCE gene in chromosom- conformation polymorphism analysis and al area 1q42-q43 [ 4,5 ]. (who.int)
Evolutionary genomic analysis revealed two novel mutations, ORF1b:V2354F and a premature stop codon, ORF7a:Q94*, identified in a cluster of SARS-CoV-2 Delta isolates collected from vaccinated individuals in Colorado. (biomedcentral.com)
These results show there is an emerging sub-lineage of the ancestral Delta variant circulating in the U.S. As mutations emerge in constellations, those with a potentially beneficial advantage to the virus may continue to circulate while others will cease. (biomedcentral.com)
The authors posit that selective pressure from host immune responses, specifically those that are antibody mediated, are responsible for frequent mutations and deletions in the NTD of circulating SARS-CoV-2 lineages. (biomedcentral.com)
NGS assays targeted to a panel of genomic regions associated with known pathogenic mutations offer several advantages over traditional sequencing methods, including lower cost and rapid assessment of many regions. (biomedcentral.com)
We find that together with loss-of-function mutations in p53 and, to some degree, potentiated by cMyc overexpression, Dusp4 deletion aids in cell cycle checkpoint escape while simultaneously potentiating hallmarks of replicative stress, including multinucleation and Chk1 phosphorylation. (biomedcentral.com)
This device is a real-time PCR test for the qualitative detection of exon 19 deletions and exon 21 (L858R) substitution mutations of the epidermal growth factor receptor (EGFR) gene in DNA derived from formalin-fixed paraffin-embedded (FFPET) human non-small cell lung cancer (NSCLC) tumor tissue. (pdffox.com)
Targeted next-generation sequencing (NGS) technology detects specific mutations that can provide treatment opportunities for colorectal cancer (CRC) patients. (preprints.org)
Approximately 97.9% (142) of patients showed somatic mutations. (preprints.org)
Stage 3 and 4 patients with ATM mutations (p=0.023) had shorter OS, and FBXW7 mutation was significantly associated with shorter DFS (p=0.002). (preprints.org)
Thus, we report here the first characterization of activating and inactivating mutations in eelLHR and we show that these mutations have similar effects as those reported for mammalian LHRs. (preprints.org)

Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. (biomedcentral.com)
Moreover, both native SMRT and Oxford Nanopore DNA methylation sequencing require microgram levels of native, unamplified DNA as input. (biomedcentral.com)
with Nanopore, TAPS allows 5mC to be sequenced as a normal base, avoiding the need for training data and complex analysis, and thereby improving detection accuracy. (biomedcentral.com)
With emphasis on MinION Nanopore sequencing, cDNA-direct and target-enrichment (amplicon-based) sequencing approaches were validated in parallel. (frontiersin.org)
We concluded that depending on the focus of a research project, amplicon-based MinION Nanopore sequencing can be an ideal cost- and time-effective alternative to Illumina in producing optimal genome coverage. (frontiersin.org)
Whole genome sequencing was performed on thirty-four SARS-CoV-2 positive samples using the Oxford Nanopore MinION. (biomedcentral.com)

Overall our results indicate a rather limited comparability between community structures investigated and determined using different primer pairs as well as between metagenome and 16S rRNA gene amplicon based community structure analysis. (frontiersin.org)

1q23.1 homozygous deletion and downregulation of Fc receptor-like family genes confer poor prognosis in chronic lymphocytic leukemia. (cancerindex.org)
Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within the organisms. (wikipedia.org)
Such features also challenge assembly across genomic islands containing niche-defining genes that drive ecological speciation. (peerj.com)
Methods A next-generation sequencing (NGS) panel was created for the human TRPV1 gene and in addition, for the leukotriene receptors BLT1 and BLT2 recently described to modulate TRPV1 mediated sensitisation processes rendering the coding genes LTB4R and LTB4R2 important co-players in pharmacogenetic approaches involving TRPV1. (researchgate.net)
The NGS workflow was based on a custom AmpliSeq™ panel and designed for sequencing of human genes on an Ion PGM™ Sequencer. (researchgate.net)
A cohort of 80 healthy subjects of Western European descent was screened to evaluate and validate the detection of exomic sequences of the coding genes with 25 base pair exon padding. (researchgate.net)
This identified approximately 140 chromosome loci where nucleotides deviated from the reference sequence GRCh37 hg19 comprising the three genes TRPV1, LTB4R and LTB4R2. (researchgate.net)
It is suitable for large-scale sequencing of TRPV1 and functionally related genes. (researchgate.net)
We cloned and sequenced a 924-nt fragment that covered part of the 5′UTR and the VP4/VP2 capsid genes. (plos.org)
Meanwhile, Paraphase is a tool capable of phasing haplotypes from highly homologous, medically significant genes, such as SMN1/SMN2 , within targeted sequencing HiFi datasets 3 . (seqanswers.com)
When comparing the design flexibility, performance, and cost of the targeted enrichment methods to whole-exome sequencing, the RNA-bait hybridization capture enrichment gene panel offers the better solution for interrogating the aortopathy genes in a clinical laboratory setting. (biomedcentral.com)
We propose a method for targeted identification of allelic variation in rice grain type genes by combining targeted sequencing of mixed samples and whole genome sequencing. (springeropen.com)
The method has the characteristics of low detection cost, short detection period, and flexible detection of traits and genes. (springeropen.com)
We have shown that the pathogen deletes two key genes (pfhrp2 and pfhrp3 ), its antigens commonly recognised by RDT. (lshtm.ac.uk)
We investigate whether the deletion of the genes cause the parasite to be less fit by measuring its ability to grow and develop in the labouratory. (lshtm.ac.uk)
3. Investigating whether malaria pathogens with the deletion of the two key genes have any impact on the outcome and severity of disease. (lshtm.ac.uk)

Together, these findings suggest that VirION provides a high throughput and cost-effective alternative to fosmid and single-virus genomic approaches to more comprehensively explore viral communities in nature. (peerj.com)
however, one of the most common current approaches is with long-read sequencing technologies. (seqanswers.com)
OGM adds value as a standalone technique to anchor and span complex repeats and can serve as an orthogonal quality check complement to sequencing-based phasing approaches. (seqanswers.com)
2003 ). The NHGRI has categorized new sequencing approaches into those that offer near-term and revolutionary benefits with a 100-fold cost reduction per base pair (bp) within the next five years. (springer.com)

Here, we describe the somatic and germline changes of pediatric MDS using whole exome sequencing, targeted amplicon sequencing, and/or RNA-sequencing of 46 pediatric primary MDS patients. (nature.com)
In this study we perform tumor and normal whole exome sequencing (WES) on 32 pediatric primary MDS patients and targeted sequencing on another 14 cases through a single institution study focused on defining the genomic landscape of pediatric MDS. (nature.com)
remarks regarding the screening like WGS (whole genome sequencing), WES (whole exome sequencing, gene panel (incl. (lovd.nl)
Methods We performed whole exome sequencing in a consanguineous family with heterotaxia. (bmj.com)
This data was also compared to whole-exome sequencing data from ten individuals. (biomedcentral.com)

In 1927, Muller showed that X-ray treatment could increase the mutation rate in a Drosophila population by 15,000% [ 10 ], and a year later, Stadler observed a strong phenotypic variation in barley seedlings and sterility in maize tassels after exposure to X-rays and radium [ 11 , 12 ]. (hindawi.com)
Results We identified a homozygous 2 bp deletion in MMP21, encoding matrix metalloproteinase-21, as the sole coding mutation that segregated with the phenotype. (bmj.com)
Conclusions Results suggested that the NGS approach based on AmpliSeq™ libraries and Ion Personal Genome Machine (PGM) sequencing is a highly efficient mutation detection method. (researchgate.net)
Biotech Europe GmbH, Freiburg, Germa- cation of the virus in cells heterozygous for ny) was used to extract genomic DNA the mutation proceeded at an intermediate from white blood cells following the lysis rate [ 5 ]. (who.int)
ATM mutation was significantly associated with higher tumor stage (p=0.012) and shorter overall survival (OS) (p=0.041). (preprints.org)

DNA isolation, genotyping of simple tandem repeat polymorphisms, PCR amplification and sequencing were performed using standard techniques 2 and all simple tandem repeat polymorphisms were amplified in the presence of 10% glycerol and 5% DMSO after labelling one oligonucleotide primer with 32 P and analysed on a 6% polyacrylamide gel. (bmj.com)
Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
Due to its high processivity and strand displacement activity, REPLI-g DNA Polymerase is capable of amplifying up to 100 kb without dissociating from the DNA template and minimizes unequal sequence and locus representation compared with PCR-based amplification methods. (qiagen.com)
Taking advantage of the large number of virus DNA sequences in public databases to select conserved sites for primer design is an optimal way to tackle the difficulties in virus genome amplification. (biomedcentral.com)
Polymerase chain reaction showed that compared with the success rate of the most popular primers for whole genome amplification of HBV, one set of primers for full length genome amplification and four sets of walking primers showed significant improvement. (biomedcentral.com)
Because of the existence of quasispecies, the only way to obtain HBV haplotype sequences is through full length genome amplification and clone-sequencing instead of assembling the PCR sequences of several amplified fragments of the genome. (biomedcentral.com)

The REPLI-g Mitochondrial DNA Kit overcomes this limitation by enriching for mitochondrial DNA sequences in total DNA samples. (qiagen.com)

Enriched samples were barcoded, pooled, and sequenced on the Illumina HiSeq2000 platform. (biomedcentral.com)

All affected persons had homozygous deletion of 12 bp (155-166del) in exon 3 of the TBCE gene. (who.int)

Additional strategies can be implemented to help improve phasing such as using sequence data from parents to bin long reads into their respective parental haplotypes during the assembly process, known as trio-binning, and/or by including long-range chromosomal contact information from Hi-C sequencing," Korlach stated. (seqanswers.com)
Regardless of the classification, approximately 55% of AML patients show chromosomal abnormalities [ 16 ] . (encyclopedia.pub)

Genomic and cDNA sequence alignments as well as chromosome localization using nullisomic-tetrasomic lines provided strong evidence for three expressed gene copies located on homoeolog chromosomes for TaSK1 as well as for TaSK2 . (biomedcentral.com)

However, compared to other flaviviruses, USUV has received less research attention and there is therefore limited access to whole-genome sequences and also to in-depth phylogenetic and phylodynamic analyses. (frontiersin.org)
Within this sub-lineage we generated whole genome sequences of a cluster of SARS-CoV-2 isolates obtained from vaccinated individuals in CO between June 3 and June 21, 2021. (biomedcentral.com)
In order to identify optimal sites for primer design, we utilized 1020 whole genome sequences in public databases (NCBI, EMBL and DDBJ) and 103 sequences in our laboratory, and developed a program BxB to select conserved regions as candidates for primer design. (biomedcentral.com)

In the latter, archaeal primer pair ArchV34 showed the highest similarity to the archaeal community structure compared to observed by the metagenomic approach and thus appears to be the appropriate for analyzing archaeal communities in biogas reactors. (frontiersin.org)
Here we established a low-cost, low-input, high throughput alternative sequencing and informatics workflow to improve viral metagenomic assemblies using short-read and long-read technology. (peerj.com)

Using Oligo software, Kuwait identified as KCS type 1 showed version 3.4, 3 polymerase chain reaction that they fulfilled the criteria for SSS, and (PCR) primer sets to amplify exons 2, 3, and might be considered as such. (who.int)

The method adds a large amount of genetic information as a basis for complete analysis of TRPV1 ion channel genetics and its functional consequences. (researchgate.net)

Transient suppression or CRISPR/Cas9-mediated deletion of mmp21 in zebrafish embryos induced cardiac looping defects, with concomitant disruption of laterality markers in the lateral plate mesoderm and disrupted notch signalling in vitro and in vivo. (bmj.com)
Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
In vitro kinase assays showed that TaSK1 and TaSK2 possessed kinase activity. (biomedcentral.com)

In this study, CRISPR/Cas9-based genome-editing technology was applied to inactivate eBSV sequences in the host plantain genome. (nature.com)
Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. (wikipedia.org)
They accidentally cloned part of a CRISPR sequence together with the "iap" gene (isozyme conversion of alkaline phosphatase) from the genome of Escherichia coli which was their target. (wikipedia.org)
In 2001, Mojica and Ruud Jansen, who were searching for a additional interrupted repeats, proposed the acronym CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) to alleviate the confusion stemming from the numerous acronyms used to describe the sequences in the scientific literature. (wikipedia.org)
showed evidence that CRISPR repeat regions from the genome of Archaeoglobus fulgidus were transcribed into the long RNA molecules that were subsequently processed into unit-length small RNAs, plus some longer forms of 2, 3, or more spacer-repeat units. (wikipedia.org)
In 2005, yogurt researcher Rodolphe Barrangou discovered that Streptococcus thermophilus, after iterative phage challenges, develops increased phage resistance, and this enhanced resistance is due to the incorporation of additional CRISPR spacer sequences. (wikipedia.org)
The Cas proteins showed helicase and nuclease motifs, suggesting a role in the dynamic structure of the CRISPR loci. (wikipedia.org)
To interrogate the pathogenic potential of the discovered variant, we used an in vivo system in which the potential of the candidate gene to induce L-R asymmetry was tested by transient suppression and CRISPR/Cas9-induced deletions. (bmj.com)

In contrast to harsh bisulfite treatment, TAPS preserves long DNA molecules over 10 kb, which is vital for long-read sequencing. (biomedcentral.com)

We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. (biomedcentral.com)
We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome. (biomedcentral.com)

The identification of chromosome 1 translocations and deletions is a rare and poorly investigated event in chronic lymphocytic leukemia (CLL). (cancerindex.org)
For the phylogenetic relationships among the arboreal members of Cercopithecus , several types of molecular data, including X and Y chromosome DNA sequences, protein sequences and karyotypes, recognize a clade containing a cephus species group and a mitis group. (biomedcentral.com)

The application of next-generation sequencing technology in microbial community analysis increased our knowledge and understanding of the complexity and diversity of a variety of ecosystems. (frontiersin.org)
They recognized the diversity of the sequences that intervened in the direct repeats among different strains of M. tuberculosis and used this property to design a typing method that was named spoligotyping, which is still in use, today. (wikipedia.org)
Two samples were co-infected with HRV-A and HRV-B or HRV-C. By comparative analysis of the VP4/VP2 sequences of the 66 HRVs, we showed a high diversity of strains in HRV-A and HRV-B species, and a prevalence of 51.5% of strains that belonged to the recently identified HRV-C species. (plos.org)
Our study showed a high diversity of HRV strains that cause bronchitis and pneumonia in children. (plos.org)
Breakthrough is expected because vaccines are not 100% effective, but the underlying causes of breakthrough afforded by genomic diversity within this lineage are not yet fully elucidated. (biomedcentral.com)
The beta diversity of the two rodent species showed significant differences across the Mediterranean, Irano-Turanian, and Sudanian regions. (microbial-ecology.net)

At diagnosis, the FLT3- ITD status is routinely assessed by fragment analysis, providing information about the length but not the position and sequence of the ITD. (oncotarget.com)
This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
When analyzing a fragment of the 5′ UTR, we characterized at least two subspecies of HRV-C: HRV-Cc, which clustered differently from HRV-A and HRV-B, and HRV-Ca, which resulted from previous recombination in this region with sequences related to HRV-A. The full-length sequence of one strain of each HRV-Ca and HRV-Cc subspecies was obtained for comparative analysis. (plos.org)

In this setting next generation sequencing (NGS) offers several advantages over traditional molecular techniques. (biomedcentral.com)
To achieve this, we continuously work on the development of new and fit-for-purpose molecular and genomic tools to detect and monitor these new malaria parasite population. (lshtm.ac.uk)
Dr Beshir teach molecular and genomic evolution and he is a tutor on Medical Parasitology course and Malaria DL module and is involved in the teaching of Miscroscopic Diagnosis of Malaria Parasites and Molecular Biology. (lshtm.ac.uk)
Baseline prevalence of molecular marker of sulfadoxine/pyrimethamine resistance in Ebonyi and Osun states, Nigeria: amplicon deep sequencing of dhps-540. (lshtm.ac.uk)

Consequently, established primers specifically amplifying the archaeal 16S ribosomal gene region are scarce compared to the variety of primers targeting bacterial sequences. (frontiersin.org)
However, a disadvantage of this primer pair was its low specificity for the archaeal domain in the experimental application leading to high amounts of bacterial sequences within the dataset. (frontiersin.org)

Reverse sequence analysis showed that the MLLT1 gene was fused to the 16p11.2 region. (cancerindex.org)
For the future direction, we need the in-depth genome sequence information and analysis for most of the mammals, including human to fully understand genome variation of economic traits, genetic susceptibility to diseases, and pharmacogenomics of drug response. (springer.com)

SMRT sequencing can directly detect DNA modifications using polymerase kinetics information, but requires a minimum of 250× per strand coverage to detect 5mC [ 11 ], largely defeating the purpose of long-read sequencing. (biomedcentral.com)
Quantitative polymerase chain reaction validation of three complete vMAGs revealed their independence from host bacterium abundance. (microbial-ecology.net)

Multiple copies of eBSV viral sequences have become integrated as direct and inverted tandem repeats at a single locus in the B genome of the host during viral infection 5 . (nature.com)

Due to the overlapping phenotypes presented by these disorders genetic sequencing is often required for accurate diagnosis and appropriate clinical intervention. (biomedcentral.com)

In contrast to adult MDS, little is known about the genomic landscape of pediatric MDS. (nature.com)

ii) captured abundant, highly microdiverse virus populations, and (iii) captured more and longer genomic islands. (peerj.com)

We confirmed the close relationship of their structural proteins but showed apparent additional recombination events in the 2A gene and 3′UTR of the HRV-Ca strain. (plos.org)

The biology of GISTs has been widely investigated at the genomic level. (biomedcentral.com)
Dr Beshir is a genomic epidemiologist with a background in Biology, Medical Parasitology and Biomedical Science. (lshtm.ac.uk)

2002, while the estimated number could be deletion in the CCR5 gene [ 5,6 ]. (who.int)

Results The amplicons covered approximately 97% of the target sequence. (researchgate.net)
The results showed that there was extensive variation in granulotype. (springeropen.com)

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