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  • antibodies
  • We compared the bactericidal activity of recombinant sets of chimeric IgG monoclonal antibodies against two important outer membrane meningococcal vaccine antigens: PorA and factor H binding protein (FHbp). (asm.org)
  • On the other hand, the IgG3 hinge-truncated antibodies IgG3m15 and IgGm17 showed higher bactericidal activity than both IgG1 and IgG3 regardless of the target antigen. (asm.org)
  • Thus, the Fc region of IgG3 antibodies appears to have an enhanced complement-activating function, independent of their long hinge region, compared to IgG1 antibodies. (asm.org)
  • Immune protection against invasive meningococcal disease depends on recognition of bacterial surface antigens by antibodies, followed by activation of complement, leading to degradation of the bacteria by bacteriolysis, also named serum bactericidal activity (SBA). (asm.org)
  • By using monoclonal hapten (4-hydroxy-3-nitrophenacetyl [NP/NIP])-specific antibodies of all four IgG isotypes, we have demonstrated that IgG1 and IgG3 are best in inducing complement-mediated cellular lysis and IgG1 performs better than IgG3 when the antigen concentration on the target cells is high, while IgG3 performs better than IgG1 when the antigen concentration on the target cells is low ( 20 , 21 ). (asm.org)
  • In separate studies, IgG3 antibodies also showed higher SBA than IgG1 antibodies when the target antigen was sparsely expressed (as in the case of FHbp) ( 22 ), but IgG1 antibodies were more bactericidal than IgG3 antibodies when the target antigen was highly expressed, such as for PorA ( 23 ). (asm.org)
  • For the production of antibodies against defined cell surface antigens such as these, the best approach has been described to include chemical conjugation of the antigen to a highly immunogenic carrier protein plus the use of a potent immunologic adjuvant. (aacrjournals.org)
  • In renal transplant, the allograft is responsible for triggering many innate and adaptive immune mechanisms, either mediated by cells, such as macrophages and lymphocytes, or by soluble components, such as antibodies and the complement system, which can ultimately lead to graft rejection [ 1 ]. (hindawi.com)
  • In contrast, in a discordant combination complement activation by preexisting antibodies in the recipient or by the vascular endothelium of the donor organ causes HAR of the xenograft . (springer.com)
  • regulatory
  • Alissafi T, Hatzioannou A, Ioannou M, Sparwasser T, Grün JR, Grützkau A, Verginis P. De novo- induced self-antigen-specific Foxp3+ regulatory T cells impair the accumulation of inflammatory dendritic cells in draining lymph nodes. (btcure.eu)
  • Expression of type-I IFN depends on the activation of Interferon Regulatory Factor - 3 (IRF3) and IRF7 via I kappa B kinase (IKK) epsilon and Tank Binding Kinase 1 (TBK1). (intechopen.com)
  • binds
  • The greater activity of the truncated IgG3 hinge mutants indicates that the long hinge of IgG3 seems to downregulate through an unknown mechanism the inherent increased complement-activating capability of IgG3 Fc when the antibody binds to a sparse antigen. (asm.org)
  • Non-functional V1 fHbps were further characterised by binding and structural studies, and shown in non-transgenic and transgenic mice (expressing chimeric fH that binds fHbp and precisely regulates complement system) to retain their immunogenicity. (pubmedcentralcanada.ca)
  • inhibition
  • Complement inhibition enables renal allograft accommodation and long-term engraftment in presensitized nonhuman primates. (nih.gov)
  • Inhibition of complement activation with the Yunnan-cobra venom factor (Y-CVF) successfully prevented accelerated antibody-mediated rejection and resulted in successful accommodation and long-term renal allograft survival in most presensitized recipients. (nih.gov)
  • These results suggest that the complement inhibition-based strategy may be valuable alternative in future clinical cross-match positive or ABO-incompatible transplantation. (nih.gov)
  • CD11c
  • Simultaneous administration of Poly(I:C) and antigen (Ag) OVA significantly increased a minor population of CD8 + T cells, that express CD11c in lymphoid and tumor sites. (biomedcentral.com)
  • role of complement
  • In the present study, we determined the role of complement in renal allograft accommodation in donor skin-presensitized nonhuman primates under conventional immunosuppression. (nih.gov)
  • Gene
  • CD8-LV mediated stable in vitro and in vivo reporter gene transfer as well as efficient transfer of genes encoding TCRs recognizing the melanoma antigen tyrosinase. (bloodjournal.org)
  • Whereas TCR gene-modified T cells recognize peptide-major histocompatibility complex (pMHC), CAR recognize antigen in an MHC-independent fashion. (bloodjournal.org)
  • Crry
  • In this study, we describe a novel function for the mouse RCA type I membrane protein Crry/p65 as a costimulatory molecule in CD4 + T cell activation. (jimmunol.org)
  • Analysis of intracellular intermediaries shows that Crry/p65-CD3 coligation enhances certain TCR/CD3-mediated signals, producing increased early tyrosine phosphorylation of many substrates and enhanced activation of the mitogen-activated protein kinase, extracellular signal-related kinase. (jimmunol.org)
  • CD20
  • In addition, we were able to show that the level of apoptosis induced in human lymphoma cell lines was in accordance with the extent of both surface CD20 clustering and caspase-3 activation. (aacrjournals.org)
  • vaccine
  • To characterize the safety and immunogenicity of a heptavalent antigen-keyhole limpet hemocyanin (KLH) plus QS21 vaccine construct in patients with epithelial ovarian, fallopian tube, or peritoneal cancer in second or greater complete clinical remission. (aacrjournals.org)
  • FHbp
  • IgG1 showed higher bactericidal activity than IgG3 when directed against PorA (an abundant antigen), while IgG3 was more bactericidal than IgG1 when directed against FHbp (a sparsely and variably distributed antigen). (asm.org)