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  • cDNA
  • Spot blot hybridization of the brain phosphorylase cDNA to laser-sorted human chromosome fractions, and Southern blot analysis of hamster/human hybrid cell line DNA reveals that the exact homolog of the newly cloned cDNA maps to chromosome 20, but that a slightly less homologous gene is found on chromosome 10 as well. (duke.edu)
  • Graphical
  • The presented graphical representation is available for visualizing characterizations of a single DNA sequence and identifying similarities and differences among several DNAs. (hindawi.com)
  • nucleotides
  • The above models were all based on individual nucleotides such that it was easy to inspect compositions and distributions of four bases directly, but difficult to dinucleotides or trinucleotides in DNA sequences. (hindawi.com)
  • The incorporation of repeats and overlapping of connected sequence segments by a few nucleotides implied that extensive rearrangements by homologous recombination might be involved in evolution of this region. (biomedcentral.com)
  • next generation
  • Recent advances in sample preparation and analysis for next generation sequencing have made it possible to profile and discover new miRNAs in a high throughput manner. (frontiersin.org)
  • Indirect sampling of miRNAs in blood and cerebrospinal fluid, because of their small amounts of total RNA, have not easily leant themselves for profiling by next generation sequencing (NGS). (frontiersin.org)
  • NGS-resources: Fwd: Analysis of high-depth sequence data for studying viral diversity: a comparison of next generation sequencing platforms using Segminator II. (blogspot.com)
  • Next generation sequencing provides detailed insight into the variation present within viral populations, introducing the possibility of treatment strategies that are both reactive and predictive. (blogspot.com)
  • Given that next generation data is increasingly important in the analysis of drug-resistance and vaccine trials, this software will be useful to the pathogen research community. (blogspot.com)
  • Among them sequence data is increasing at the exponential rate due to advent of next-generation sequencing technologies. (wikipedia.org)
  • The advent of next-generation sequencing technologies has resulted in generation of voluminous sequencing data. (wikipedia.org)
  • Methods
  • Analysis methods must therefore also be sensitive. (biomedcentral.com)
  • DNA sequencing is one of the most widely used methods for analysing DNA and has been successfully used to analyse and detect mutations in DNA derived from formalin-fixed paraffin-embedded tumours (FF-PETs) for many years. (biomedcentral.com)
  • Glycoside hydrolases may also be classified by sequence or structure based methods. (wikipedia.org)
  • Social sequence analysis is a special application of sequence analysis, a set of methods that were originally designed in bioinformatics to analyze DNA, RNA, and peptide sequences. (wikipedia.org)
  • After being introduced to the social sciences in the 1980s and a period of slow growth during the 1990s, social sequence methods have become increasingly prevalent. (wikipedia.org)
  • Social network analysts have begun to turn to sequence methods and concepts to understand how social contacts and activities are enacted in real time, and to model and depict how whole networks evolve. (wikipedia.org)
  • The use of sequence methods was initially met with criticism by sociologists who objected to the descriptive and data-reducing orientation of early sequence methods, as well as to a lack of fit between bioinformatic sequence methods and uniquely social phenomena. (wikipedia.org)
  • Since 2000 there has been a surge of interest in refining sequence methods, leading to some major improvements in social sequence methods. (wikipedia.org)
  • Many of the methodological developments in social sequence analysis came on the heels of a 2000 special issue devoted to the topic in Sociological Methods & Research, which hosted a debate over methods for comparing sequences. (wikipedia.org)
  • That debate has given rise to several methodological innovations that address limitations of sequence comparison methods that were developed in the 20th century. (wikipedia.org)
  • Sociological Methods & Research organized another special issue on social sequence analysis in 2010, leading to what scholars have dubbed the "second wave" of sequence analysis. (wikipedia.org)
  • This idea serves as an implicit motivation behind social sequence analysts' use of optimal matching, clustering, and related methods to identify common "classes" of sequences at all levels of social organization, a form of pattern search. (wikipedia.org)
  • this is in contrast to vertical methods that make comparisons across several different genetic sequences. (wikipedia.org)
  • novel
  • This paper constructed a novel injection from a DNA sequence to a 3D graph, named circular helix-like curve (CHC). (hindawi.com)
  • In addition, due to the development of novel sequencing platforms and chemistries, each with implicit strengths and weaknesses, it will be helpful for researchers to be able to routinely compare and combine data sets from different platforms/chemistries. (blogspot.com)
  • Structural
  • Based on three-dimensional structural similarities, the sequence-based families have been classified into 'clans' of related structure. (wikipedia.org)
  • different
  • We explored the changes in total mapped reads and differential expression results generated by three different software packages: miRDeep2, miRNAKey, and miRExpress and two different analysis packages, DESeq and EdgeR. (frontiersin.org)
  • Segminator II was developed to allow for the efficient comparison of data sets derived from different sources. (blogspot.com)
  • representation
  • But to our regret, there are crosses and overlaps of the spatial curve in the representation in [ 5 ], and the Z-curve might cause a loop if the frequencies of the four bases present in the sequence are the same as pointed out by Tang et al. (hindawi.com)
  • species
  • Most lethal cases as well as several survivors have been confirmed by DNA PCR analyses to have high-level systemic infections by one of seven distinct species of elephant herpesviruses named EEHV1 to EEHV7 that form a single clade within the Proboscivirus genus ( 1 , 2 , 7 - 10 ). (asm.org)
  • Additional DNA-DNA hybridization studies or genomic and proteomic comparison experiments of the two species could lead to more definitive results. (wikipedia.org)
  • linkage
  • Renin was the first blood pressure (BP) quantitative trait locus mapped by linkage analysis in the rat. (ahajournals.org)
  • One strategy for mapping blood pressure (BP) variants is by linkage analysis using inbred rat strains. (ahajournals.org)
  • 1 Although useful, linkage analysis is limited to localizing a large (10-30 cM) quantitative trait locus (QTL) that is associated with BP, but cannot further reduce the list of potential causative variant(s) within the QTL. (ahajournals.org)
  • samples
  • Deep sequencing allows for massive parallel quantification and evaluation of the miRNA composition in a large number of samples at one time. (frontiersin.org)
  • Using the Illumina NGS platform, we routinely barcode and sequence up to 175 small RNA samples per flow cell on the HiSeq 2000. (frontiersin.org)
  • The ARMS assays maximised the number of samples that could be analysed when both the quality and quantity of DNA was low, and improved both the sensitivity and speed of analysis compared with sequencing. (biomedcentral.com)
  • ARMS was more sensitive and robust at detecting defined somatic mutations than DNA sequencing on clinical samples where the predominant sample type was FF-PET. (biomedcentral.com)
  • They perform well on FF-PET-derived DNA and their sensitivity makes them ideal for the analysis of heterogeneous tumour samples. (biomedcentral.com)
  • We demonstrate its usage by comparing large data sets from 12 influenza H1N1 samples sequenced on both the 454 Life Sciences and Illumina platforms, permitting quantification of platform error. (blogspot.com)
  • detection
  • Each tissue must be assessed independently for the depth of coverage required to adequately power detection of differential expression, weighed against the cost of sequencing that sample to the adequate depth. (frontiersin.org)
  • approaches
  • Whereas traditional approaches to the coding-vs.-non-coding problem generally relied on sophisticated pattern recognition systems that were first trained on small inputs and then run over the entire sequence (Ohler, et al. (wikipedia.org)
  • similar
  • The identification of similar sequences in this report is based on clustering as described here . (rcsb.org)
  • In the table for each entity, view a list of similar sequences by selecting the link associated with the percentage cutoff. (rcsb.org)
  • tissue
  • However, the processes used to prepare tumours for standard pathology analysis for diagnosis, while preserving the tissue architecture, have a detrimental effect on DNA and RNA. (biomedcentral.com)
  • among
  • Among all the representations following Hamori's idea, the most worthy to mention is that in the year 2000, Randić and cooperators [ 5 ] constructed a new model of DNA sequences which was also based on a path in 3D space. (hindawi.com)
  • Sequence comparison among Dahl salt-sensitive, Dahl R, and Brown Norway reduced the number of candidate variants in the 2 BP loci by 42% for further study. (ahajournals.org)
  • widely
  • The most widely used approach to multiple sequence alignments uses a heuristic search known as progressive technique (also known as the hierarchical or tree method) developed by Paulien Hogeweg and Ben Hesper in 1984. (wikipedia.org)