Comet s percentage tail dna. Medical search. Frequent questions

DNA damage in HepG2 cells after TIGAR knockdown with or without treatment of epirubicin or CoCl 2 was detected by Comet assay. (nature.com)
Vitamin C was analysed at each time point by HPLC, whereas H 2 O 2 -induced MNBC DNA damage was evaluated at 0, 3 and 24 h by means of the comet assay. (cambridge.org)
Sperm DNA integrity was assessed by neutral comet assay and reported as comet extent, percentage DNA in comet tail (Tail%) and tail distributed moment (TDM). (cdc.gov)
CONCLUSIONS: Environmental exposure to carbaryl and chlorpyrifos may be associated with increased DNA damage in human sperm, as indicated by a change in comet assay parameters. (cdc.gov)
Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
The motility, fertilising ability and DNA integrity of freeze-thawed goldfish sperm was evaluated using computer-aided sperm motility analysis and the comet assay. (scialert.net)
The comet assay is a technique which can evaluate the DNA integrity of cells. (scialert.net)
Comet assay allows comparisons using a simple procedure and requires small sample size. (scialert.net)
All the samples were processed immediately and analyzed for DNA damage by single cell gel electrophoresis assay - Comet assay technique. (archive.org)
staining of SA-β-gal was performed to look for the senescent cells in irradiated NSCLC cells utilizing a senescence β-galactosidase staining package (Cell Signaling) even as we previously reported (18 19 Comet assay Natural comet assay was utilized to determine DNA-DSBs in irradiated NSCLC cells with a Comet Assay? (researchatlanta.org)
Cells were blended with Comet Assay Briefly? (researchatlanta.org)
Objectives: This study used the comet assay to assess DNA damage in buccal mucosa cells consequent to X ray radiation from panoramic radiography. (ui.ac.id)
Buccal mucosa cells were collected from all participants before exposure to IR and at 30 min or 24 h after exposure in groups 1 and 2, respectively, and subjected to a comet assay to assess DNA damage. (ui.ac.id)
Anthropometic parameters, blood lipid profiles, glucose, insulin, CRP, and baseline DNA damage using comet assay and adiponectin concentrations were determined at baseline and at 12 weeks of intervention. (elsevierpure.com)
This investigation integrates the well-established micronucleus assay in exfoliated buccal epithelial cells, urinary 8-OHdG, salivary telomere length and salivary C-reactive protein to provide a comprehensive assessment of inflammation, cytotoxicity, chromosomal aberrations and DNA damage in a cohort of 11-15 year olds. (westminster.ac.uk)
DNA fragmentation assay was done through Hoechst 33258 and acridine orange-ethidium bromide staining. (jcimjournal.com)
DNA damage was quantified through comet assay. (jcimjournal.com)
Biochemical parameters and comet assay were assessed to evaluate the efficiency of OXD-T treatment on the HCC induced animal. (walshmedicalmedia.com)
Similarly, no increase in DNA damage was observed in individual cells following BRD4770 treatment by comet assay, measuring the tail instant length (Assisting Number S6b). (innovation-ecosystems-agora.com)
We observed intracellular reactive oxygen species(ROS) level by DCFH-DA method, mitochondrial membrane potential alteration by rhodamine 123 staining and apoptotic morphological changes by acridine orang/ethidium bromine dual staining method and Hoest staining assay and comet assay. (pdfplayer.org)
In view of these considerations, this study was designed to evaluate the cytotoxic and genotoxic potential of geraniol, in an in vitro protocol, using two types of human cells: one without the ability to metabolize (peripheral blood mononuclear cells - PBMC), and the other with this capability (human hepatoma cell line - HepG2) through the comet assay and the micronucleus test. (geneticsmr.org)
At the end of a total of 45 days of exposure, mice weresacrificed, implantation sites were monitored, blood was processed to study stress parame-ters (hemoglobin, RBC and WBC count, and neutrophil/lymphocyte (N/L) ratio), the brainwas processed for comet assay, and plasma was used for nitric oxide (NO), progesterone andestradiol estimation. (nomedicalcare.com)

These spermatozoa also experienced increased mitochondrial generation of reactive oxygen species after 1 week of exposure, with elevated DNA oxidation and fragmentation across all exposure periods. (nature.com)
Furthermore, OXD-T affected the DNA fragmentation parameters (tail length, tail moment, % DNA in tail and % DNA in head of comet). (walshmedicalmedia.com)
OXD-T therapeutic administration revealed highly significant decreases in the biochemical values and DNA fragmentation parameters more than half dose of OXD-T treatment. (walshmedicalmedia.com)
Oxidative stress can lead to reduced sperm count, motility, and morphology, as well as increased sperm DNA fragmentation. (carrottopng.com)

Oxidative damage to DNA, and/or the direct adduction of reactive molecules to DNA, such as can occur with formaldehyde, is the most important intermediate outcome of chemical carcinogenesis. (nationalacademies.org)
Through ionisation of molecules, IR generates toxic free radicals, which can result in single and double-strand DNA breaks, incur oxidative damage to sugar and base residues, and cause chromosomal aberrations and mutations without distinguishing between tumour and normal tissues ( 1 , 2 ). (sciendo.com)
Biomarkers of oxidative damage (DNA single-strand breaks, lipid peroxidation and free radical metabolism) and inflammation were determined using conventional biochemistry techniques. (biomedcentral.com)

The percentage of apoptotic cells in peripheral bloodstream is certainly higher in SLE sufferers than in regular healthful sufferers considerably, as well as the progression of human lupus nephritis is proportional towards the increase in the speed of apoptosis [7] directly. (findadig.com)
In 4T1 breast cancer cells, short-term starvation resulted in increased phosphorylation of the stress-sensitizing Akt and S6 kinases, increased oxidative stress, caspase-3 cleavage, DNA damage, and apoptosis. (kingdomfasting.com)
The DNA damage response (DDR) is a mechanism that protects cells against radiation-induced oxidative DNA damage by causing cell cycle arrest and apoptosis. (researchsquare.com)
Ionizing radiation is well known to induce oxidative DNA damage, such as DNA double-strand breaks (DSBs), and consequently trigger the DNA damage response (DDR), including cell cycle arrest and apoptosis. (researchsquare.com)

The co-existing risk factors and old age may increase the baseline DNA damage in the patients with head and neck cancers. (archive.org)

Spectrography has confirmed their presence on celestial bodies including the dwarf planet Ceres, asteroid 101955 Bennu, and comet Tempel 1, as well as Jupiter's moon Europa. (wikipedia.org)

DNA integrity of sperm estimated on the basis of single gel electrophoresis and DNA damage, especially single and double strand breaks ( Warnecke and Pluta, 2003 ) are reflected in a comet like dispersion of the DNA fragments. (scialert.net)

Comet assays demonstrate that RV and IR mixed treatment causes even more DNA double-strand breaks (DSBs) than IR or RV treatment by itself. (researchatlanta.org)
Double-strand breaks (DSBs) were assessed by comparing the percentages of tail DNA in output images obtained before and after X ray exposure. (ui.ac.id)
The mutagenic effects of ROS are well-known and include oxidative base modifications and DNA single and double strand breaks. (westminster.ac.uk)

Open up in another window Shape 2 Aftereffect of raising native DNA foundation pair molar percentage for the fluorescence emission spectra of Flavonoids. (pkc-inhibitor.com)
Occurrence of comet tail and increased fluorescence of Hoechst after 24 h of drug exposure revealed significant DNA nick generation and chromatin condensation. (jcimjournal.com)

Colony formation, β-gal staining, immunofluorescence, comet and homologous recombination (HR) assays and RNA-Seq analysis were used to determine the effects of AP4 inactivation. (biomedcentral.com)

Aftereffect of Reactive Air Scavengers for the Flavonoids-Induced DNA Damage in Permeabilized Lymphocytes To be able to concur that the flavonoid-induced lymphocyte DNA damage outcomes from ROS era, the effect continues to be studied by us of some scavengers of ROS on comet tail length formation by plant polyphenols [5]. (pkc-inhibitor.com)

This study investigated the role of TIGAR in DNA damage response (DDR) induced by genotoxic drugs and hypoxia in tumor cells. (nature.com)
To define a role of TIGAR in DNA damage, TIGAR expression was knocked down with siRNA in HepG2 cells. (nature.com)
Epirubicin, a DNA damaging anticancer agent and CoCl 2 , which was used to imitate hypoxia condition, was applied to induce DNA damage in TIGAR knockdown HepG2 cells. (nature.com)
Even though cancer cells are more vulnerable to IR than normal cells due to rapid cell division and inefficient DNA repair, both sustain DNA damage. (sciendo.com)
Further studies uncovered the fact that percentage of senescence-associated β-galactosidase (SA-β-gal)-positive senescent cells was markedly higher in cells treated with IR in conjunction with RV weighed against cells treated either with IR or RV by itself recommending that RV treatment enhances IR-induced early senescence in lung tumor cells. (researchatlanta.org)
Collectively these outcomes demonstrate that RV-induced radiosensitization is certainly connected with significant boost of ROS creation DNA-DSBs and senescence induction in irradiated NSCLC cells recommending that RV treatment may sensitize lung tumor cells to radiotherapy via improving IR-induced premature senescence. (researchatlanta.org)
We applied a range of molecular and cellular techniques to measure cell survival, cell cycle progression, protein expression and DNA damage combined with a transcriptomic micro-array approach in D283-MED cells, for global gene expression analysis in acute and chronic hypoxic conditions. (biomedcentral.com)
Whilst U87MG shared the same dampened p53 activity, upon chemotherapeutic drug treatment in chronic hypoxic conditions, these cells used a different mechanism, independent of the DNA damage pathway. (biomedcentral.com)
As expected, all of the flavonoids induced significant DNA harm, and led to increased tail measures of comets, in comparison to control cells ( 0.01). (pkc-inhibitor.com)
Desk 2 Aftereffect of scavengers of reactive air varieties on flavonoid-induced mobile DNA damage in permeabilized cells. (pkc-inhibitor.com)
In contrast, studies reporting DNA damage in bacteria, induction of SCE in mammalian cells or tests in inappropriate test systems such as yeasts are considered to contribute far less to the overall assessment and are therefore not further discussed. (europa.eu)
Furthermore, Mediator of DNA damage Checkpoint 1 ( MDC1 ), a central component of the DNA damage response and a known target of miR-22-3p, displayed decreased expression in AP4 -deficient cells. (biomedcentral.com)
Inhibition of miR-22-3p or ectopic MDC1 expression reversed the increased senescence, DNA damage, CIN and defective HR observed in AP4 -deficient CRC cells. (biomedcentral.com)
Inset, determined percentage of cells in each phase. (innovation-ecosystems-agora.com)
The DNA guardian protein p53 plays the most important role in DDR: it promotes DNA repair and the elimination of cells that are unable to repair the damage caused by oxidative stresses, including radiation. (researchsquare.com)

There are a variety of well-established genotoxicity tests that can evaluate acquired DNA damage and chromosomal instability. (westminster.ac.uk)
Inactivation of AP4 in CRC cell lines resulted in increased spontaneous and c-MYC-induced DNA damage, chromosomal instability (CIN) and cellular senescence. (biomedcentral.com)
This instability is a percentage for economy and Handbook of Identification wave system from a Other Acoustic Radiation Force Impulse( ARFI) that is characterized as a obtained instability improved at the nonlinear wave of a HIFU pore tuning in helpAdChoicesPublishersLegalTermsPrivacyCopyrightSocial discrepancy. (treasuresresalestore.com)

0.05) correlation between DNA integrity and the maximum Duration of Forward Progressive Swimming (DFPS) ability of cryopreserved goldfish sperm. (scialert.net)
0.05, in comparison to untreated whole lymphocytes (comet tail length = 3.43 0.13). (pkc-inhibitor.com)
0.05, in comparison to untreated permeabilized lymphocytes (comet tail length = 3.85 0.17). (pkc-inhibitor.com)

In addition to lipid and protein damage, ROS (namely secondary, injurious derivatives) have the capability of causing DNA single-stand breaks (SSB) and base oxidation [ 10 ]. (biomedcentral.com)

Rats with thioacetamide- induced liver fibrosis were grouped into: group I control that did not receive thioacetamide and h-ASC, group II received thioacetamide and saline i.v., group III received thioacetamide and h-ASC 1 x 106/ kg i.v. via tail vein. (bvsalud.org)

Every aspect of the complex DNA structure is susceptible to oxidation, and reactive species have been directly implicated in more than 30 base modifications and 70 lesions [ 12 ]. (biomedcentral.com)

As discussed in Chapter 5 , numerous compounds identified in e-cigarette aerosols can form reactive oxygen species (ROS), and/or can be converted to reactive intermediates capable of binding to DNA. (nationalacademies.org)

Many classic mechanisms of chemoresistance have been widely researched from a genetic perspective, such as the abnormality of DNA damage repair (DDR) related genes, increased detoxification activity, the repressed chromatin state and drug efflux promoted by the high expression of multidrug resistance-associated protein 2 [ 5 ]. (ijbs.com)
The c-Jun N-terminal kinase (JNK), belonging to the MAPK family (also known as stress-activated protein kinase SAPK), is activated in response to various stimuli, such as infection, oxidative stress, cytotoxic drugs, cytoskeletal changes and DNA damage [ 11 ]. (ijbs.com)
This study was conducted to determine weight loss effects on the plasma adiponectin levels and clinical parameters including oxidative DNA damage and C-reactive protein (CRP) in overweight-obese subjects. (elsevierpure.com)
Extreme era of reactive air species (ROS) gets the potential to initiate harm to lipids, protein, and DNA [3]. (findadig.com)

The phosphopentose pathway (PPP), which converts glucose-6-phosphate to ribose-5-phosphate for synthesis of nucleotides and NADPH to reduce DNA damage caused by ROS was reported to be activated in DDR 2 . (nature.com)
ATM and ATR are important regulators of cell-cycle arrest caused by DNA damage, including senescence.13,14 To investigate the mechanism DZ2002 further underlying cell-growth inhibition induced by BRD4770, we examined the effect of BRD4770 treatment on ATM and ATR pathway activation. (innovation-ecosystems-agora.com)

Genetic changes can occur at many levels, from gain or loss of entire chromosomes to a mutation affecting a single DNA nucleotide [ 3 ]. (walshmedicalmedia.com)

The results indicate that sublethal effects during the freeze-thawing procedure compromised sperm fertilising ability, DNA integrity, velocity linearity and the duration of swimming. (scialert.net)
straight-line velocity (VSL) linearity (LIN), DNA integrity and fertilising ability. (scialert.net)
DNA integrity and to a lesser degree the simple assessment of the DFPM of thawed sperm may be reliable indicators of sperm integrity for the development and the rapid assessment of fish sperm cryopreservation protocols. (scialert.net)
This interaction can further generate extraneous ROS accounting for even more harm to the integrity of DNA even. (pkc-inhibitor.com)

In summary, AP4, miR-22-3p and MDC1 form a conserved and coherent, regulatory feed-forward loop to promote DNA repair, which suppresses DNA damage, senescence and CIN, and contributes to 5-FU resistance. (biomedcentral.com)

The genetic material is fundamentally the double helix of the DNA molecule. (whale.to)
Any substance that damages DNA or chromosomes, or changes genetic activity, is genotoxic because it is acting on the same material, i.e. the DNA molecule. (whale.to)

D) Quantification of PANC-1 cell growth in smooth agarose by DNA measurement (see Methods). (innovation-ecosystems-agora.com)

In conclusion, modest weight loss improved metabolic parameters including blood lipids, insulin resistance, CRP, and DNA damage, but did not increase plasma levels of adiponectin in overweight-obese subjects. (elsevierpure.com)

A negative correlation between Tail% and TDM was present only when stratified by comet extent, suggesting that Tail% and TDM may measure different types of DNA damage within comet extent strata. (cdc.gov)
Emerging evidence indicates a correlation between increased adiposity and markers of oxidative DNA damage. (westminster.ac.uk)

Findings of other biomarkers indicative of DNA strand breaks in obesity include -H2AX foci, micronuclei and comet tails. (westminster.ac.uk)

Here, we challenge this view and show that HPV-18 E2 over-activates the Spindle Assembly Checkpoint (SAC) and induces DNA breaks in mitosis followed by aneuploidy. (docksci.com)
The ingestion of a high fat meal induces postprandial oxidative stress, inflammation and a rise in DNA damage that remains unaltered by one hour of preceding exercise. (biomedcentral.com)

However, it remains unexplored if TIGAR plays an important role in DNA damage and repair. (nature.com)
Here, we present our findings on a novel role of TIGAR in DNA damage and repair. (nature.com)
Earlier studies ( 7 , 10 - 12 ) have already described DNA damage and repair in human blood samples for each of the three anaesthetics and IR doses usually applied in therapy, but none has yet addressed single-dose exposure to a combination of VAs and IR, even though IR at sufficient doses has a similar mechanism of action as VA ( 13 ) and their combinations might have synergistic effects. (sciendo.com)

It is observed that both biological effects and epidemiological effects appear to be the same or very similar from ELF exposure and from RF/MW exposures, including calcium ion efflux, melatonin reduction, DNA strand breakage, chromosome aberrations, leukaemia, brain cancer, breast cancer, miscarriage and neurological effects. (whale.to)
Results: A statistically signifcant (p 0.014) increase in the percentage of tail DNA was observed at 30 min after exposure, but not at 24 h (p 0.29). (ui.ac.id)

Urinary levels of insecticide metabolites and DNA damage in human sperm. (cdc.gov)
The present study explored whether environmental exposures to carbaryl and chlorpyrifos are associated with DNA damage in human sperm. (cdc.gov)
However, excessive ROS levels can lead to oxidative stress, which can damage sperm DNA and impair male fertility. (carrottopng.com)

The scolytines include over 6000 species worldwide (only a relatively small percentage of which, it should be noted, are recognised as significant pests). (fieldofscience.com)
Most Sapphire Olive Eggers lay olive-colored eggs, but there will be a small percentage of brown egg layers. (onporte.be)

Studies showed that there is an increased primary DNA damage even before the commencement of any modality of treatment in cancer patients which is further increased by the treatment. (archive.org)

They usually have a tail, but because of the Araucana genes, some birds may be rumpless. (onporte.be)

Results: The comet length parameter, head diameter, and tail length were found to be increased when compared to baseline sample. (archive.org)
Desk 2 summarizes the observations from an test where in fact the results had been examined by us of catalase, Thiourea and SOD on flavonoid-induced DNA degradation in permeabilized lymphocytes. (pkc-inhibitor.com)

DNA replication, synthesis and subsequent cell division has been linked to each of the stages of the carcinogenesis process [ 4 , 5 ]. (walshmedicalmedia.com)

The percentage of DNA in head parameter of post-RT sample was decreased when compared to baseline sample All these findings are indicative of DNA damage following radiotherapy. (archive.org)

One such marker is 8- OH-2-deoxy Guanosine (8-OHdG), a DNA lesion that is potentially mutagenic. (westminster.ac.uk)

Thus, one mechanism of epirubicin combined TACE treatment for HCC was through induction of synergistic DNA damage. (nature.com)

We do been that Close sciences feel Pathological Other comet on experimental and crazy equation of electron-positron Remains. (treasuresresalestore.com)

Findings from this study aim to establish the applicability of personalized, early detection of acquired DNA damage as a pre-cancerous biomarker in obesity and other pre-pathological conditions. (westminster.ac.uk)

Epirubicin was reported to block DNA replication either through direct DNA damage or indirectly through inhibition of replication proteins such as topoisomerase. (nature.com)

The aim of the present investigation was to study the effect of the intake of a single portion of blood orange juice (BOJ, 300 ml, providing 150 mg vitamin C) on mononuclear blood cell (MNBC) DNA damage, compared with a drink supplemented with the same amount of vitamin C (C-drink) or sugars (S-drink). (cambridge.org)
Few EO present only one single component in high percentage. (geneticsmr.org)
It has been estimated that a single DNA structure is directly attacked 2 × 10 4 times per day, the majority of which is attributed to free radicals [ 11 ]. (biomedcentral.com)

Yet, the acute effects of prior exercise and PHTG on DNA damage remains unknown. (biomedcentral.com)

This suggests a straightforward setting of binding of flavonoids to DNA. (pkc-inhibitor.com)

Anatomy2

Diseases6

Chemicals and Drugs3

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes4

Assay22

Fragmentation4

Oxidative damage3

Apoptosis4

Baseline DNA damage1

Tempel1

Electrophoresis1

Double-strand3

Fluorescence2

Assays1

Lymphocyte1

Cells15

Instability3

0.053

Lipid1

Liver1

Oxidation1

Reactive oxygen1

Protein4

Pathway2

Nucleotide1

Integrity4

Senescence1

Molecule2

Agarose1

Parameters1

Correlation2

Biomarkers1

Induces2

Repair3

Exposure2

Sperm3

Small percentage2

Cancer1

Genes1

Results2

Synthesis1

Indicative1

Mutagenic1

Induction1

Experimental1

Findings1

Replication1

Single3

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Suggests1