AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
FermentationChromatography, High Pressure LiquidCoccidiostatsSpectrometry, Mass, Fast Atom BombardmentChromatography, Thin LayerChromatographyStreptomycesChromatography, GelGelsChromatography, AffinityPhysicochemical PhenomenaChemistry, PhysicalMagnetic Resonance SpectroscopyChromatography, Ion ExchangePlant ExtractsMolecular StructureChromatography, GasMolecular WeightSilica GelChromatography, DEAE-CelluloseAnti-Bacterial AgentsChromatography, LiquidElectrophoresis, Polyacrylamide GelMicrobial Sensitivity TestsChemistryBlood Grouping and CrossmatchingChemical PhenomenaChromatography, AgaroseMolecular Sequence DataKineticsHydrogen-Ion ConcentrationAmino AcidsSubstrate SpecificityMass SpectrometryAmino Acid SequenceGas Chromatography-Mass SpectrometryMethodsSpectrophotometry, InfraredSepharoseIsoelectric FocusingChemical FractionationHydroxyapatitesChromatography, PaperCarbohydratesBlood Group AntigensCattleIsoelectric PointTemperatureSpectrophotometry, UltravioletPlants, MedicinalImmunodiffusionCarbohydrate SequenceEscherichia coliElectrophoresis, Gel, Two-DimensionalChemical PrecipitationRabbitsAmmonium SulfateLiverHydrolysisBase SequenceDrug StabilityOligosaccharidesElectrophoresisCentrifugation, Density GradientTrypsinGlycoside HydrolasesHot TemperatureChromatography, Reverse-PhaseEnzyme StabilityElectrophoresis, DiscCloning, MolecularDurapatitePeptide FragmentsElectrophoresis, Agar GelSolubilityIsoenzymesRecombinant ProteinsProtein BindingMacromolecular SubstancesIndicators and ReagentsCarbohydrate ConformationGlycoproteinsImmunoelectrophoresisSpectrophotometryUltracentrifugationSwineFractional PrecipitationPronaseBacterial ProteinsPeptidesGlycosidesSpectrometry, Mass, Electrospray IonizationBinding SitesElectrophoresis, Gel, Pulsed-FieldPolysaccharidesImmune SeraSequence Homology, Amino AcidCell LineSpecies SpecificityCations, Divalent
ElectrophoresisPurificationProteinsSilica gelsFiltrationSeparationPreparativeResinReproducibilityAffinityResinsPurity silicaAdsorptionAnalyticalHydrophilicCommercially availableResidueChemically-bondedPorousIrregularHPLC ColumnLiquidFlameIncreased separationsMeshRetentionAgaroseCrudeCompoundsRigidRangePolymerParticle SizeProcessAnalysisEfficiencyAdditionReversed-phase columnsProteinIndustryDataIndustrial
Electrophoresis4
  • Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. (cshlpress.com)
  • A structured controlled vocabulary for the annotation of sample processing and separation techniques in scientific experiments, such as, and including, gel electrophoresis, column chromatography, capillary electrophoresis, centrifugation and so on. (obofoundry.org)
  • Further purification on a Mono S column followed by activity measurements and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the affinity-purified enzyme preparation contained two active heterodimeric aspartic proteinases: a larger 48k Da enzyme, consisting of 32-kDa and 16-kDa subunits and a. (lu.se)
  • Further purification on a Mono S column followed by activity measurements and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the affinity-purified enzyme preparation contained two active heterodimeric aspartic proteinases: a larger 48k Da enzyme, consisting of 32-kDa and 16-kDa subunits and a smaller one of 40 kDa, consisting of 29-kDa and 11-kDa subunits. (lu.se)
Purification6
  • The MCI Gel™ size exclusion columns and resins are based on hydrophilic polyhydroxymethacrylates suitable for the analysis and purification of water soluble polymers like oligosacharides, proteins, PEG etc. (biokal.com)
  • The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts-a critical and often neglected step in successful protein purification. (cshlpress.com)
  • Hamilton offers 21 polymer-based stationary phases as well as two silica gels (C8 and C18) to satisfy most separation/purification needs. (hamiltoncompany.com)
  • AEX chromatography resins and membrane adsorbers are frequently used for downstream purification in the biotechnology industry. (biopharminternational.com)
  • various drawbacks have been identified in the purification techniques of ion resin adsorption ( 12 , 13 ), silica gel column chromatography ( 14 ) and supercritical extraction ( 15 ). (spandidos-publications.com)
  • In this study, supercritical fluid chromatography (SFC) was successfully utilized for the purification of actinomycin X2 and actinomycin D from a marine derived Streptomyces sp. (bvsalud.org)
Proteins3
  • Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. (gbiosciences.com)
  • Analytical and micropreparative high-performance gel chromatography of proteins with a short column. (nist.gov)
  • When a solution of proteins (e.g. hemolysate) is passed through the column, the glycated component is retained by the complexing of its diol groups with the boronate. (cdc.gov)
Silica gels2
  • Their silica gels are ideal for preparative chromatography, from laboratory to pilot-plant processes and production scale. (fishersci.com)
  • In addition to performance, SiliCycle™'s silica gels also ensure consistency, reliability and reproducibility. (fishersci.com)
Filtration3
  • Silica gel may be used in separation of single-walled carbon nanotube (SWNT) by simple filtration. (sigmaaldrich.com)
  • Shim-pack Diol series is a kind of gel filtration chromatography (GFC) column. (shimadzu.com)
  • The isolation protocol involved ion-exchange chromatography and gel filtration on a Superdex75 column. (nih.gov)
Separation9
  • It is type of high purity Silica gel in fine powder form which is use during separation of mixture of components. (silicagelsuppliers.com)
  • The analysis of individual components usually involves separation of the components by column chromatography (i.e., packed or capillary column) followed by a suitable quantification method. (cdc.gov)
  • Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. (shimadzu.com)
  • Since this method allows low-cost separation by automation of the separation process and by permitting reuse of the gel, industrial applications of separated metallic and semiconducting SWCNTs utilizing their characteristics are expected. (go.jp)
  • Takeshi Tanaka (Research Scientist) and Hiromichi Kataura (Leader), the Self-Assembled Nano-Electronics Group, the Nanotechnology Research Institute (Director: Nobutsugu Minami) of AIST (President: Tamotsu Nomakuchi), developed a method for high-purity separation of SWCNTs into metallic SWCNTs and semiconducting SWCNTs by using a column packed with agarose gel beads, as a part of Industrial Technology Research Grant Program of NEDO. (go.jp)
  • The gel-packed column can be used repeatedly, and the separation process can be easily automated. (go.jp)
  • Separation of the n-hexane fraction using silica gel column chromatography yielded the chloroform:methanol (50:1) fraction, which showed the highest tyrosinase inhibitory activity. (ijpsonline.com)
  • These diol groups provide the basis for separation of glycated and non-glycated components by boronate affinity chromatography 11a,11b,11c. (cdc.gov)
  • GC/MS analysis allows separation of compounds based on their retention time within a chromatography column and identification of each compound by the characteristic fragments into which a given chemical is broken following ionization of the compound. (medscape.com)
Preparative2
  • In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. (spandidos-publications.com)
  • Preparative high performance liquid chromatography (prep-HPLC) can shorten the production cycle, has relatively low operating costs, and is simple to carry out. (spandidos-publications.com)
Resin7
  • The columns are 7.5 mm x 600 mm (ID x L) packed with 10 µm particle size resin. (biokal.com)
  • G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. (gbiosciences.com)
  • Our specialty resins are offered in a wide variety of hardware dimensions, and while these sizes cover many applications in varying science focuses, you may need a specific Hamilton resin in a column size we don't offer as off-the-shelf. (hamiltoncompany.com)
  • This results in packed columns with larger diameter, and therefore volume, than optimal, and this improper column sizing results in greater resin requirements and increased buffer usage, both of which impact operating time and cost of goods. (biopharminternational.com)
  • To screen and characterize the analgesic components from Marasmius androsaceus , the water-soluble extracts from the mycelium of M. androsaceus were isolated by ethanol precipitation followed by macroporous resin chromatography and activity-based fractionation. (scirp.org)
  • The nematode-antagonistic compounds from the second fungus species are currently being isolated from the culture broth by extracting with Amberlite XAD resin and purifying with Toyopearl HW40 and silica gel column chromatography and with reversed-phase HPLC. (usda.gov)
  • For packing compression glass column, convenient to packing resin. (galaklc.com)
Reproducibility2
  • Shim-pack VP series ensures high column-to-column performance reproducibility, which is ideal for method development and validation. (shimadzu.com)
  • QC test probes serve a vital function in ensuring the reproducibility of modern GC columns. (chromatographyonline.com)
Affinity2
  • The corresponding enzyme was purified from grain extracts by affinity chromatography on a pepstatin-Sepharose column. (lu.se)
  • The Primus instrument is a fully automated glycohemoglobin analyzer, which utilizes the principle of boronate affinity high performance liquid chromatography (HPLC)P 11dP. (cdc.gov)
Resins5
  • On this page: List of available columns and resins, followed by some sample chromatograms. (biokal.com)
  • The performance of commonly used AEX resins and membrane adsorbers, detailing dynamic binding capacity performance, efficiency, and a new disposable option for packed bed chromatography are compared. (biopharminternational.com)
  • Multiple product formats are commercially available, including chromatography resins, which are packed into chromatography columns, and membrane adsorbers, which are supplied in self-contained plastic housings. (biopharminternational.com)
  • On the other hand, the re-use of resins is well established for traditional packed bed chromatography and this facilitates lower overall material costs. (biopharminternational.com)
  • High performance, rigid resins with a higher volumetric throughput capability provide an advantage over soft-gel resins allowing for properly sized columns with smaller footprints similar to membranes. (biopharminternational.com)
Purity silica1
  • Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns. (shimadzu.com)
Adsorption1
  • The FIA method determines saturated hydrocarbons, olefins and aromatic hydrocarbons of a sample chromatographically by adsorption and desorption in a column filled with FIA silica, in the presence of a fluorescent dye mixture. (thomassci.com)
Analytical5
  • For this September issue of The Application Notebook should be reaching our audience of analytical chemists, lab managers, and other industry professionals just as the busiest time of the year ramps up in the world of chromatography. (chromatographyonline.com)
  • In this analytical technique, a boronate such as phenylboronic acid is bonded to the surface of the column support. (cdc.gov)
  • The analytical column contains aminophenylboronic acid bonded to a porous polymer support (gel). (cdc.gov)
  • The low- and high-pressure pumps transfer reagents through the analytical column, with reagent selection executed by a switching valve. (cdc.gov)
  • Over the years, technologic advances in electronics and detectors have allowed GC/MS to detect ever smaller concentrations of compounds, as has the addition of other analytical techniques, such as liquid chromatography. (medscape.com)
Hydrophilic1
  • Shim-pack WAX/WCX series columns are chemically-bonded hydrophilic silica gel based ion exchange columns. (shimadzu.com)
Commercially available1
  • The vast amount of commercially available C18 columns are constructed on a wide range of solid supports, and may exhibit ancillary options such as endcapping and aqueous compatibility (AQ). (chromatographyonline.com)
Residue1
Chemically-bonded2
  • According to the United States Pharmacopeia ( USP ), there are 858 C18 liquid chromatography phases registered under code L1 (octadecylsilane chemically bonded to porous silica or ceramic microplates, 1.5 to 10 μm in diameter, or a monolithic rod) (3). (chromatographyonline.com)
  • In 1973, Locke correctly predicted that organosilanes would transform HPLC columns via chemically-bonded phases, mentioning that polymerization of silanes would be the synthetic route taken in general with chemical reactions being carried out to produce a primary organosilane layer (5). (chromatographyonline.com)
Porous2
  • Shim-pack GIST series columns are packed with newly developed high-purity porous spherical silica. (shimadzu.com)
  • Since their inception in the 1970s, superficially porous particles (SPPs) have offered good performance and are indispensable in high-speed high performance liquid chromatography (HPLC) (1). (chromatographyonline.com)
Irregular1
  • For higher demands on the performance of column packings we recommend our high-purity irregular POLYGOPREP silicas. (thomassci.com)
HPLC Column3
Liquid7
Flame1
  • Flame ionization gas chromatography and an SE-30 column provided the fastest and most reproducible analysis. (cdc.gov)
Increased separations1
  • Designed to maximize performance of LC systems, Shimadzu's Shim-pack Velox columns with core shell technology enable you to achieve increased separations and faster analysis times on any LC platform. (shimadzu.com)
Mesh1
Retention1
  • These probes ensure that the columns have been properly deactivated, contain the correct amount of stationary phase, and have the same relative retention as the last column purchased. (chromatographyonline.com)
Agarose3
  • Agarose gel is prepared by dissolving agarose in hot water and cooling the solution until it solidifies. (go.jp)
  • AIST had developed methods for separating metallic and semiconducting SWCNTs by using agarose gel. (go.jp)
  • This method involves the use of a column packed with agarose gel beads, in which the semiconducting SWCNTs are selectively adsorbed and then eluted (Fig. 1). (go.jp)
Crude1
  • After the respective times of reaction described in Table 1 , crude products were isolated, filtered over column chromatography of silica gel, and then analysed by 1 H NMR. (hindawi.com)
Compounds3
  • Even for LC columns that claim to be designed for basic compounds, adequate resolution often can not be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long equilibration time required for low ionic strength acidic mobile phase. (shimadzu.com)
  • Shim-pack Velox PFPP (Pentafluorophenylpropyl) provides an alternative selectivity to C18 columns and is suitable for the analysis of halogenated compounds, positional isomers and charged bases. (shimadzu.com)
  • The choice of individual compounds in these test mixes varies widely and can have profound consequences on the performance of a column in the users' applications. (chromatographyonline.com)
Rigid1
Range2
  • Finally With continuous focus and efforts of our R&D Department, We are Introducing our new range of product in chromatography process. (silicagelsuppliers.com)
  • Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter LC columns, which are the next generation organic silica hybrid based columns. (shimadzu.com)
Polymer1
  • Silica gel allows a controlled fragmentation leading to form uniform polymer particles with narrow particle size distribution and high bulk density. (sigmaaldrich.com)
Particle Size1
  • Shim-pack XR Series columns use a 2.2 μm packing particle size and offer a skillful balance between resolution efficiency and pressure. (shimadzu.com)
Process4
  • It provides similar processing times and the added benefits of reusability, ease of packing at different scales in various column formats, and the ability to implement initial process design from early phase manufacturing to commercial manufacturing, reducing overall costs and time to market. (biopharminternational.com)
  • AEX products are used for polish chromatography in product FT mode to bind product-related and process impurities. (biopharminternational.com)
  • In most cases, traditional soft-gel FT columns are sized for the optimization of volumetric throughput to improve operating flow rate and decrease process bottlenecks, rather than sized for actual impurity binding capacity. (biopharminternational.com)
  • Late-phase process redesign or a complete switch to packed bed chromatography may be required due to high consumable costs or limited product formats not allowing for linear scale-up. (biopharminternational.com)
Analysis2
  • Shim-pack FC-ODS is an ideal column to shorten your analysis time using conventional HPLC. (shimadzu.com)
  • The authors recommend a total sampling volume of 10 liters in an atmosphere containing 5mg/m3, and the desorption and analysis of the glass fiber plug, used to hold the silica gel in place, along with the silica gel for total quantitation of both DBPC dust and vapor. (cdc.gov)
Efficiency1
  • Controllable surface area, diversity in particle morphology, and higher efficiency values when compared to other support materials are just some of the advantages of silica-based columns. (chromatographyonline.com)
Addition1
Reversed-phase columns1
  • Shim-pack MAqC-ODS I reversed-phase columns are packed with a silica gel containing metal and added octadecylsilyl group. (shimadzu.com)
Protein1
  • Furthermore, gel column chromatography on Sephadex G-150 indicated that the elution curves of the carbohydrate and protein overlapped significantly in sample P ( Figure 1 ). (scirp.org)
Industry1
  • These advancements on solid supports have contributed great benefits to every industry where chromatography takes place, but novel approaches to functionalization of such platforms has remained stagnant for decades. (chromatographyonline.com)
Data1
Industrial1
  • The authors conclude that DBPC can be sampled in the industrial atmosphere using silica gel tubes. (cdc.gov)