• Most in vitro diagnostic assays are developed based on molecular or immunodetection technologies, including polymerase chain reaction (PCR), enzyme-linked immunosorbent assays (ELISA), chemiluminescent immunoassays (CLIA), lateral flow, cytology, and immunohistochemistry. (sigmaaldrich.com)
  • In ELISA-based assays, antibodies are attached to a chromogenic enzyme, which in the presence of substrate produces a quantitative, colorimetric signal. (sigmaaldrich.com)
  • High purity α-L-Arabinofuranosidase ( Bifidobacterium adolescentis ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • Colorimetric assays or detection use UV absorption or enzymatic color reaction. (biovisi.com)
  • We used colorimetric assays to measure nutrient concentrations. (mendeley.com)
  • Find our optimized NADPH regeneration system and catabolism buffer to pair with your in vitro assays using recombinant enzymes and subcellular fractions. (tebubio.com)
  • RapidStart™ is a versatile system that supports extended metabolism of test articles by NADPH-dependent drug-metabolizing enzymes in subcellular fractions for in vitro drug metabolism assays. (tebubio.com)
  • Assays using microsomes, S9 and recombinant CYPs (Bactosomes) can be supported with RapidStart™ to support the long-term metabolism of test articles by these enzymes by ensuring that NADPH is not the limiting reagent. (tebubio.com)
  • Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. (enzolifesciences.com)
  • Development of suitable enzymatic assays for characterization of putative lignin active enzymes is an important step towards improving our understanding of the catalytic activities of relevant enzymes. (biomedcentral.com)
  • Enzyme assays of a laccase and a MnP on phenolic and nonphenolic β-aryl ether substrates revealed different primary reaction pathways due to the availability of the phenoxy radical intermediates. (biomedcentral.com)
  • Our assay provides a wealth of information on bond cleavage events not available using conventional colorimetric assays and can easily be carried out in microliter volumes and the quantitative analysis of product formation and kinetics is rapidly achieved by NIMS. (biomedcentral.com)
  • The kit is formulated to give sensitive detection of Pi, providing an alternative to hazardous radioactive methods and other less sensitive colorimetric assays. (cmscorp.co.kr)
  • Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry and immunocytochemistry, where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA). (wikipedia.org)
  • Quantitative determination was done by colorimetric techniques, using liquid culture supernatants to determine the respective substrate degradation. (javeriana.edu.co)
  • Quantitative determination was possible for all the evaluated enzymes except lipases. (javeriana.edu.co)
  • Here, the rolling circle amplification method, termed rolling circle enhanced enzyme activity detection (REEAD) assay that allows for the quantitative measurement of topoisomerase 1 (TOP1) activity in a simple, fast, and gel-free manner is presented.By cleaving and ligating a specially designed DNA substrate, TOP1 converts a DNA oligonucleotide into a closed circle, which becomes the template for rolling circle amplification, yielding ~103 tandem repeat rolling circle products. (au.dk)
  • Herein, we propose a dual-mode Cu 2 O nanoparticles (NPs) based biosensor for glucose analysis based on colorimetric assay and laser desorption/ionization mass spectrometry (LDI MS). Cu 2 O NPs exhibited excellent peroxidase-like activity and served as a matrix for LDI MS analysis, achieving visual and accurate quantitative analysis of glucose in serum. (frontiersin.org)
  • Uric Acid Assay Kit is a quantitative colorimetric uric acid determination at 590 nm. (tebubio.com)
  • Quantitative colorimetric detection. (rndsystems.com)
  • The TiterTACS Colorimetric Apoptosis Detection Kit is a TUNEL based assay utilizing specific in situ labeling technology that enables a 96 well microplate format for high throughput quantitative detection of apoptosis. (rndsystems.com)
  • free antibody is washed away, and a secondary anti-human antibody linked to an enzyme signal is added. (wikipedia.org)
  • Multiple probing of the same membrane with alternative antibody probes can be performed using substrates that produce different colors, such as blue and red (Blake et al. (bio-rad.com)
  • Of these, fluorescent, biotin, and enzyme labeling reagents are used most frequently for antibody labeling. (dojindo.com)
  • Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. (assaygenie.com)
  • Antibody-HRP conjugates are superior to antibody-AP conjugates with respect to the specific activities of both the enzyme and antibody. (ecmbio.com)
  • The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. (eaglebio.com)
  • Acid is added to the sample well to stop the reaction, and the colorimetric endpoint is read on a microplate spectrophotometer set to the appropriate light wavelength. (cdc.gov)
  • The assay is based on an enzyme-coupled reaction that detects inorganic phosphate. (immunochemistry.com)
  • The substrate is converted to Hydrogen peroxide in presence of inorganic phosphate in a coupled enzymatic reaction. (immunochemistry.com)
  • Colorimetric AP systems use soluble 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitroblue tetrazolium (NBT) as substrates to produce a stable reaction product that will not fade (see Figure below). (bio-rad.com)
  • Many classical enzymatic assay methods involve the use of synthetic colorimetric substrates and measuring the reaction rates using a spectrophotometer. (nih.gov)
  • An array of chromogenic, fluorogenic and chemiluminescent substrates are available for use with HRP conjugated secondary antibodies HRP is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. (ecmbio.com)
  • A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. (eaglebio.com)
  • We show that the reaction of laccase and MnP with phenolic substrate yields products that arise from the cleavage of the carbon-carbon single bond between the α-carbon and the adjacent aryl carbon, consistent with the mechanism for producing phenoxy radical as reaction intermediates. (biomedcentral.com)
  • To facilitate understanding of reaction kinetics, the reaction time course for laccase activity on the phenolic substrate (I) was generated by the simultaneous measurement of all products at different time points of the reaction. (biomedcentral.com)
  • The ETK (Phospho-Tyr566) Colorimetric Cell-Based ELISA Kit can detect Phospho-ETK expression in as little as 5,000 HeLa cells. (antikoerper-online.de)
  • The 4E-BP1 (Phospho-Thr36) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect 4E-BP1 protein phosphorylation and expression profile in cells. (assaygenie.com)
  • In the presence of H 2 O 2 , HRP catalyzes the oxidation of the substrate into a product that is visible on a blot. (bio-rad.com)
  • An important area of this research involves using metal nanoparticles as artificial enzymes, known as nanozymes, which exhibit peroxidase-like activity enabling them to catalyse the oxidation of substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), giving a colorimetric response. (strath.ac.uk)
  • This enzyme catalyzes the hydroxylation of L-Tyrosinase to L-3,4-dihydroxyphenylalanine (L-DOPA) and oxidation of L-DOPA to DOPA-quinone, at the initial process of melanogenesis. (japsonline.com)
  • Soluble methane monooxygenase (sMMO) is a multicomponent bacterial enzyme that catalyzes the oxidation of methane to methanol, as well as oxidizing many other adventitious substrates. (shu.ac.uk)
  • The M184V mutant showed relaxed regioselectivity and a novel oxidation product with the substrate mesitylene which may have implications for substrate trafficking. (shu.ac.uk)
  • A random mutagenesis experiment was devised and a colorimetric screen for the oxidation of triaromatic compounds was used to screen mutant libraries for activity towards anthracene and phenanthrene. (shu.ac.uk)
  • Membrane-bound proteins are generally detected using secondary antibodies that are labeled with radioisotopes or colloidal gold, or that are conjugated to fluorescent molecules (fluorophores) or an enzyme such as alkaline phosphatase (AP) or horseradish peroxidase (HRP). (bio-rad.com)
  • The study of pNPP and PPi hydrolysis by the enzyme revealed that the optimum pH of actuation for pNPP was 10.5, while for PPi, which is considered the true substrate of alkaline phosphatase, was 8.0, close to the physiological value. (bvsalud.org)
  • Alkaline phosphatase (or ALP) is another enzyme commonly measured when liver dysfunction is suspected. (aacc.org)
  • Reporter molecules include horseradish peroxidase, alkaline phosphatase enzymes and fluorophores. (kangle.skin)
  • Alkaline phosphatase (ALP) is a calcium- and phosphatebinding protein and a phosphor-hydrolytic enzyme. (bvsalud.org)
  • The total amount of BChE was quantified by a sensitive colorimetric signal originating from a sandwich immunochromatographic assay utilizing PtPd nanoparticles as a colorimetric probe, which exhibited excellent catalytic activity for phenols. (cdc.gov)
  • DAB and 4CN are commonly used chromogenic substrates for HRP. (bio-rad.com)
  • Qualitative determination of enzymatic activities was done by measuring hydrolysis halos in agar plates with their corresponding substrate. (javeriana.edu.co)
  • Enzymes hydrolyzing water-soluble short-chain triacylglycerols to corresponding fatty acids and glycerol are termed esterases (EC 3.1.1.1), and enzymes catalyzing the hydrolysis of carboxylic ester bonds at the lipid-water interface are termed lipases (EC 3.1.1.3). (iisertvm.ac.in)
  • The activities of these enzymes usually are estimated using tributyrin and triolein as substrates, and the product of hydrolysis (i.e., the fatty acids released) is quantified. (iisertvm.ac.in)
  • In the case of kinetic parameters, the enzyme showed a "Michaelian" behavior for pNPP and PPi hydrolysis. (bvsalud.org)
  • In individuals with normal plasma levels of normally functioning pseudocholinesterase enzyme, hydrolysis and inactivation of approximately 90-95% of an intravenous dose of succinylcholine occurs before it reaches the neuromuscular junction. (medscape.com)
  • Several substrates were employed in the biochemical characterization of recombinant AXHd3. (megazyme.com)
  • This expression system was used to make a site directed mutants F188Aand N116G in order to complement previous site directed mutant studies, as well as a recombinant wild type mutant in order to asses the activity of the new expression system which is comparible to the wild type enzyme. (shu.ac.uk)
  • An enzyme substrate which permits the measurement of peptide hydrolase activity, e.g. trypsin and thrombin. (nih.gov)
  • Moreover, the detection of protein amount (by Western blot or immunohistochemistry) is often insufficient to provide information for cancer diagnosis, whereas the measurement of enzyme activity represents a valuable biomarker. (au.dk)
  • Measurement of enzyme activity also allows for the diagnosis and potential treatment of pathogen-borne diseases. (au.dk)
  • Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. (assaygenie.com)
  • The principle of the platform is based on the simultaneous measurement of the total amount of the enzyme and enzyme activity to biomonitor exposure to organophosphorus (OP) pesticides. (cdc.gov)
  • In particular, peroxidase, which oxidizes transparent colorimetric substrates to become a colored product in the presence of hydrogen peroxide, is the most common enzyme that is used in colorimetric bioassays. (k-erc.eu)
  • Remarkably, it is important to fabricate the easily prepared nanomaterials-based peroxidase-like colorimetric biosensors for glucose analysis. (frontiersin.org)
  • Two LMEs, laccase from the polypore mushroom Trametes versicolor , and manganese peroxidase (MnP) from white rot fungus Nematoloma frowardii , were tested for catalytic activity against the two model substrates. (biomedcentral.com)
  • for example, peroxidase-mimicking nanozymes demonstrate oxidase-like activity that oxidizes colorimetric substrates in the absence of hydrogen peroxide, which keeps them away from precisely detecting the target materials, such as hydrogen peroxide. (k-erc.eu)
  • The nitrogen and boron co-doped graphene was also applied to the colorimetric detection of acetylcholine, which is an important neurotransmitter and successfully detected the acetylcholine even better than the nature peroxidase. (k-erc.eu)
  • Get the essential reagents to perform colorimetric detection of proteins on western blots with the convenience of premixed buffers and enzyme substrate kits. (flinnsci.com)
  • However, natural enzymes consisting of proteins are unstable against temperature and pH, hard to synthesize, and costly. (k-erc.eu)
  • Nanozymes, on the other hand, do not consist of proteins, meaning the disadvantages of enzymes can be overcome with their robustness and high productivity. (k-erc.eu)
  • In CLIA, an enzyme converts a substrate into a chemiluminescent signal that is emitted as visible or near visible light. (sigmaaldrich.com)
  • The theory behind several commonly used western blotting detection methods such as colorimetric, chemiluminescent and fluorescent methods, and other less common methods, such as chemifluorescence, autoradiography, and immunogold labeling methods are highlighted below. (bio-rad.com)
  • In these methods, when the enzyme substrate is added, either a colored precipitate is deposited on the blot (colorimetric detection) or a chemiluminescent or fluorescent product is formed and the light signal is captured on film or with a digital imaging system (see figure above). (bio-rad.com)
  • HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry. (ecmbio.com)
  • Some limitations of HRP colorimetric detection systems are decreased sensitivity when compared to AP colorimetric detection systems, fading of blots upon exposure to light, inhibition of HRP activity by azide, and nonspecific color precipitation. (bio-rad.com)
  • Enzyme activity was assayed using Cl- and SCN- as the substrates. (willamette.edu)
  • One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of arabinose per minute from wheat arabinoxylan (10 mg/mL) in sodium phosphate buffer (100 mM), pH 6.0 at 40 o C. (megazyme.com)
  • The enzyme showed the highest activity toward wheat arabinoxylan oligosaccharides. (megazyme.com)
  • We have developed a colorimetric assay format for the determination of lipase activity, using sodium salt of tetra sulfonatophenyl porphyrin (Na-TSPP) as a chromogenic indicator that can also overcome the problem of the toxic nature of various indicators that are being used. (iisertvm.ac.in)
  • Activity staining was done to prove that the biochemical activity of the enzymes was preserved after electrophoresis. (iisertvm.ac.in)
  • The antioxidant activity was conducted by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay and tyrosinase inhibitory activity was conducted by colorimetric enzymatic assay. (japsonline.com)
  • The Km value was around 0.6mM for pNPPase activity and ranged from 0.01 to 0.11mM for PPase activity, indicating that the enzyme has a higher affinity for this substrate. (bvsalud.org)
  • 1997). A range of site directed mutants were created at the 110 position and screened for activity with a number of aromatic substrates. (shu.ac.uk)
  • M184V and C151T showed small changes in regioselectivity and reduced activity with most substrates. (shu.ac.uk)
  • The F282L mutant produced a stable enzyme which had no activity with any of the substrates tested, showing Phe282 is important for the enzyme function. (shu.ac.uk)
  • The diagnosis is confirmed by a laboratory assay demonstrating decreased plasma cholinesterase enzyme activity. (medscape.com)
  • Partial deficiencies in inherited pseudocholinesterase enzyme activity may be clinically insignificant unless accompanied by a concomitant acquired cause of pseudocholinesterase deficiency. (medscape.com)
  • Patients with only partial deficiencies of inherited pseudocholinesterase enzyme activity often do not manifest clinically significant prolongation of paralysis following administration of succinylcholine unless a concomitant acquired cause of pseudocholinesterase deficiency is present. (medscape.com)
  • The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. (tebubio.com)
  • The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. (tebubio.com)
  • The PARP2 Colorimetric Activity Assay Kit is designed to measure PARP2 activity for screening and profiling applications. (tebubio.com)
  • The key to the PARP2 Colorimetric Activity Assay is the biotinylated substrate. (tebubio.com)
  • Methods used to measure the relative activity of a specific enzyme or its concentration in solution. (nih.gov)
  • Note: Use of sodium azide as preservative substantially inhibits the enzyme activity of HRP. (ecmbio.com)
  • The BChE activity was estimated by another colorimetric signal using the Ellman assay. (cdc.gov)
  • The enzymes of the antigen 85 complex (Ag85A, B, and C) possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. (helmholtz-hzi.de)
  • The novel colorimetric assay is based on the quantification of glucose from the degradation of trehalose, which is the product from catalytic activity of antigen 85A. (helmholtz-hzi.de)
  • The PTP1B Colorimetric_x000D_Assay Kit is designed to measure PTP1B activity for screening and profiling applications, in a_x000D_homogeneous assay with no time-consuming washing steps. (microbiologystudents.com)
  • This PhosphoWorks Phosphate Assay Kit has been developed for measuring the activity of any Pi-generating enzyme. (cmscorp.co.kr)
  • 1. Bernal C, Palacin C, Boronat A, Imperial S. (2005) A colorimetric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol 4-phosphate synthase activity. (cmscorp.co.kr)
  • 3. Mahuren JD, Coburn SP, Slominski A, Wortsman J. (2001) Microassay of phosphate provides a general method for measuring the activity of phosphatases using physiological, nonchromogenic substrates such as lysophosphatidic acid. (cmscorp.co.kr)
  • Through this study, we have secured core technologies to synthesize nanozymes that have high enzyme activity along with selectivity. (k-erc.eu)
  • HRP systems have an advantage over other detection systems in that both the enzyme conjugate and colorimetric detection substrates are economical. (bio-rad.com)
  • Cell Technology's Phosphate detection kit provides a simple, one-step fluorimetric or colorimetric method for determination of phosphate in serum and plasma samples. (immunochemistry.com)
  • The determination of amylolytic, cellulolytic, pectinolytic and proteolytic enzymatic profiles of each of the Fusarium isolates assessed suggests their capacity to degrade these substrates, irrespectively of their origin. (javeriana.edu.co)
  • Since then, a number of enzyme systems and detection reagents have evolved. (bio-rad.com)
  • Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. (tebubio.com)
  • Next, the PARP2 biotinylated substrate is incubated with an assay buffer that contains the PARP2 enzyme. (tebubio.com)
  • Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader. (tebubio.com)
  • In general, the most known and used methods are Western Blots (WB), Enzyme-Linked Immune Sorbent Assay (ELISA), Lateral-Flow Assay (LFA), and Dot-Blot Assay (DBA) and have been applied in the detection of carcinogenic compounds, pathogenic and allergenic microorganisms using antibodies [8]. (peertechzpublications.com)
  • Previously, we have successfully built an assay platform based on glycan substrates containing a charged perfluorinated tag and nanostructure-initiator mass spectrometry to study carbohydrate active enzymes, especially various glycosyl hydrolyses. (biomedcentral.com)
  • Two β-aryl ether bond containing model lignin dimer substrates, designed to be suitable for studying the activities of lignin-modifying enzymes (LMEs) by nanostructure-initiator mass spectrometry (NIMS), were successful synthesized. (biomedcentral.com)
  • A new assay procedure has been developed for studying lignin-modifying enzymes by nanostructure-initiator mass spectrometry. (biomedcentral.com)
  • Typically an enzyme substrate is added to a buffer solution containing enzyme and the rate of conversion of substrate to product is measured under controlled conditions. (nih.gov)
  • However, the current methods mainly depend on natural enzymes, which are unstable, hard to prepare, and expensive, limiting the extensive applications in clinics. (frontiersin.org)
  • However, the natural enzymes are unstable, hard to prepare and expensive limiting the extensively clinical applications ( Ambati and Jachak, 2021 ). (frontiersin.org)
  • These abnormal alleles may result in a failure to produce normal amounts of the enzyme or in production of abnormal forms of pseudocholinesterase with altered structure and lacking full enzymatic function, as described below. (medscape.com)
  • The enzyme cleaves the substrate, liberating the pNA, which absorbs light at 405 nm. (moleculardevices.com)
  • The moss called Physcomitrella patens contains a putative SAM-dependent halide methyltransferase (PpHOL) enzyme that has been partially purified. (willamette.edu)
  • In this study, we investigated the responses of soil bacterial communities, enzyme activities and putative functional pathways to the effects of prescribed fire in a paired remnant and restored prairie system located in southwest Michigan, USA. (mendeley.com)
  • Detection using TACS-Sapphire, a non-toxic colorimetric substrate, allows both kinetic and endpoint readings. (rndsystems.com)
  • Colorimetric detection is considered a medium-sensitivity method when compared to radioactive or chemiluminescence detection. (bio-rad.com)
  • Depending on the nucleotide incorporation during the amplification, there is the possibility of different readout methods, from fluorescence to chemiluminescence to colorimetric. (au.dk)
  • Secondary antibodies conjugated to fluorophores are gaining popularity and can be directly visualized and captured with a compatible imager, without the need for additional liquid substrate (see Fluorescence Detection ). (bio-rad.com)
  • Nitrocellulose is easy to use and provides adequate data for most common enzyme indicator experiments. (kangle.skin)
  • The Merocodia Insulin ELISA is a two-site enzyme immunoassay utilizing the direct sandwich technique with two monoclonal antibodies directed against separate antigenic determinants of the insulin molecule. (cdc.gov)
  • A Double Sandwich Monoclonal Enzyme Immunoassay for Detection of Hepatitis B Surface Antigen. (dojindo.com)
  • In this study, butyrylcholinesterase (BChE) was adopted as the model enzyme and ethyl paraoxon was chosen as an analyte representing OP pesticides. (cdc.gov)
  • Pseudocholinesterase deficiency is an inherited enzyme abnormality that results in abnormally slow metabolic degradation of exogenous choline ester drugs such as succinylcholine. (medscape.com)
  • The viability of lignin degradation depends on the availability of optimal lignin-modifying enzymes [ 6 , 7 , 8 , 9 ] that can efficiently degrade lignin biopolymers into simpler aromatics. (biomedcentral.com)
  • Biosensors are devices that have a recognition element (biological or synthetic) intimately associated with a transducer (piezoelectric, optical, electrochemical, colorimetric, etc. (omicsonline.org)
  • The Pyrochrome ® Assay from Associates of Cape Cod, Inc. (ACC) contains a synthetic substrate with paranitroaniline chromogen (pNA), which enables spectrophotometric quantitation. (moleculardevices.com)
  • For example, phosphatases, ATPases and several other enzymes catalyze reactions in which inorganic phosphate (Pi) is released from a substrate. (cmscorp.co.kr)
  • NADPH is a cofactor required to support reactions by drug-metabolizing enzymes such as cytochrome P450 (CYP) and flavin-containing monooxygenases (FMO). (tebubio.com)
  • The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). (biovisi.com)
  • This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. (tebubio.com)
  • Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. (enzolifesciences.com)
  • In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form. (enzolifesciences.com)
  • Human ML-1 myeloblastic leukemia cells were treated with 1 µM staurosporine and then harvested, fixed, and labeled according to the protocol in the TiterTACS In Situ Detection Kit (Catalog # 4822-96-K ) prior to colorimetric analysis. (rndsystems.com)
  • The aminotransferase enzymes, ALT and AST, are indicators of liver cell death and can be used to answer the questions: What is the extent of hepatic damage or how many liver cells died in the last 24 hours? (aacc.org)
  • Professor Lee said, "We began to study nanozymes due to their potential for replacing existing enzymes. (k-erc.eu)
  • After washing to remove unbound enzyme-labelled antibodies, a labelled substrate is added and binds to the conjugated antibodies. (cdc.gov)
  • A number of mutagenic studies were carried out on the sMMO enzyme of Methylosinus trichosporium OB3b in order to gain insight into sMMO and probe how structural aspects relate to function of the enzyme. (shu.ac.uk)
  • We assessed the extracellular enzyme activities of cellobiohydrolase, β-glucosidase, N-acetylglucosaminidase, and phosphatase using fluorescent-linked substrates (Gutknecht et al. (mendeley.com)
  • Clotting enzyme is (indirectly) activated by endotoxin. (moleculardevices.com)
  • Sensitive detection methods using enzymes to indirectly measure substances that would normally be difficult to quantitate. (nih.gov)
  • Herein, we propose a copper-based biosensor for dual-mode glucose analysis based on colorimetric assay and LDI MS ( Schem 1 ). (frontiersin.org)