AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Cell ProliferationCell CycleCell Cycle ProteinsColony-Forming Units AssayCell DivisionCell Cycle CheckpointsTumor Stem Cell AssayCell Line, TumorG1 PhaseHematopoietic Stem CellsApoptosisMitosisS PhaseCells, CulturedColony-Stimulating FactorsCyclinsBone Marrow CellsHematopoiesisCell LineCell SurvivalTumor Cells, CulturedClone CellsG2 PhaseTime FactorsCyclin-Dependent Kinase Inhibitor p21Menstrual CycleGranulocytesMolecular Sequence DataCell DifferentiationFlow CytometrySignal TransductionCyclin-Dependent Kinase Inhibitor p27Cell Transformation, NeoplasticPhosphorylationRetinoblastoma ProteinErythropoiesisCulture MediaInterleukin-3AgarTumor Suppressor Protein p53G1 Phase Cell Cycle CheckpointsBone MarrowGene Expression Regulation, NeoplasticMutationMice, NudeG2 Phase Cell Cycle CheckpointsTransfectionRNA, MessengerG0 PhaseProtein-Serine-Threonine KinasesCyclin D1Tumor Suppressor ProteinsDNA-Binding ProteinsBase SequenceMegakaryocytesBlotting, WesternAntineoplastic AgentsDNA DamageDNA ReplicationTranscription FactorsDose-Response Relationship, DrugCDC2 Protein KinaseNuclear ProteinsDown-RegulationPhenotypeRNA, Small InterferingCyclin-Dependent Kinase 2Growth SubstancesErythropoietinModels, BiologicalHeLa CellsFibroblastsCyclin EAmino Acid SequenceReverse Transcriptase Polymerase Chain ReactionCyclin-Dependent Kinase 4DNADrug SynergismHematopoietic Cell Growth FactorsGenes, cdcCell NucleusGene Expression RegulationInterphaseTranscription, GeneticCyclin AStem CellsRecombinant ProteinsNeoplastic Stem CellsCyclin BStem Cell FactorRNA InterferenceGranulocyte-Macrophage Colony-Stimulating FactorProto-Oncogene ProteinsSaccharomyces cerevisiaeErythroid Precursor CellsGene ExpressionGrowth InhibitorsCDC2-CDC28 KinasesPromoter Regions, GeneticGene Expression Profiling
AssaysApoptosisIncluding cell proliferationVitroMigrationKnockdownViabilitySquamous cell carcTranswellOverexpressionDifferentiationInvasionReplicationHematopoietic stemGenesTissues and cell linesInhibitsMetastasisRegulationSiRNATumorsProgenitorColorectal cancerEpithelial cell lineInhibitionPathwayCellularInducePromotesProgressionAlveolar Epithelial CellsTranscriptionBone marrowPathwaysMesenchymalSomatic cellsMolecularEndothelialCervical cancer cellsFlow cytometry analysisCancer cell proliferationLinesSurvival and proliferationStem cellApoptotic cellsRegulatesVivo xenograftGrowthUltraviolet radiationTranscriptomeConclusions
Assays11
  • CCK-8, plate colony formation and EdU assays were performed to assess the cell proliferation. (biomedcentral.com)
  • Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. (spandidos-publications.com)
  • Cell proliferation was monitored by MTT and soft-agar colony formation assays, cell cycle was analyzed by flow cytometry, and cell invasion was examined by transwell assay. (biomedcentral.com)
  • The MTT and colony formation assays were used to assess the proliferation potential. (biomedcentral.com)
  • Functional assays - The shRNA knockdown effect can be studied by end-point biological and biochemical assays such as cell proliferation, colony formation, cell cycle analysis, and migration based on the potential role played by each gene /protein. (genecopoeia.com)
  • Functional assays by a reporter gene/protein - Alternatively, when the endogenous transcript level of gene of interest is low and/or when qRT-PCR and western blot analysis is not feasible or possible, KD effect can be measured by co-transduction of shRNA clone with an expression clone plasmid which is transcribed into a chimeric mRNA transcript consisting of a reporter gene and the target gene ORF. (genecopoeia.com)
  • Cell functional assays, in vivo xenografts tumor growth experiments, and survival analysis were used to investigate the functional significance and mechanism of chemosensitization after SETD8 downregulation. (biomedcentral.com)
  • Colony formation assay and CCK8 assays were conducted to confirm the function of miR-221/222 in METTL3-induced cell growth in bladder cancer. (biomedcentral.com)
  • This indispensable laboratory resource will enable all cell and developmental biologists, from the graduate level upward, to confidently carry out and comprehend a wide array of cell viability assays. (cshlpress.com)
  • These strains produced and released Stx1a as measured by cytotoxicity and neutralization assays using anti-Stx/Stx1a antiserum. (cdc.gov)
  • Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)
Apoptosis20
Including cell proliferation1
  • This study examines the effect of RPL5 on the progression of colon adenocarcinoma (COAD), including cell proliferation, migration and determines its underlying molecular mechanism using CRC cell lines in vitro. (biomedcentral.com)
Vitro11
  • In vitro and in vivo experiments showed that SNHG4 overexpression markedly enhanced cell resistance to enzalutamide. (biomedcentral.com)
  • In addition, knockdown of miR‑320 in breast cancer cell lines promoted cell proliferation and invasion in vitro, whereas miR‑320 overexpression had the opposite effect. (spandidos-publications.com)
  • The effects of BCRC-3 on cancer cells were explored by transfecting with plasmids in vitro and in vivo. (biomedcentral.com)
  • Proliferation of BC cells was suppressed by ectopic expression of BCRC-3 in vitro and in vivo. (biomedcentral.com)
  • In this study, we investigated how TSPAN12 regulates OC cell proliferation both in vitro and in vivo and found that it contributed to tumor proliferation and poor prognosis in this disease through cyclin and cyclin-dependent kinase (CDK) pathways. (molcells.org)
  • MSC use frequently requires in vitro expansion, thus exposing cells to replicative senescence. (encyclopedia.pub)
  • Researchers from the University of Toronto found that fenbendazole (FZ) can disrupt microtubules, cause cell cycle arrest and kill cancer cells in human melanoma cells in the laboratory (in vitro). (cab-aurel.com)
  • Over-expression of Gas5 remarkably suppressed PTC cells proliferation in vitro and inhibited the growth of tumor cells in vivo likewise. (oncotarget.com)
  • In vitro , propofol inhibited the growth and colony-formation of cervical carcinoma cells. (aging-us.com)
  • Additionally, GEM or GEM+CDDP at low concentrations dramatically facilitated the proliferation, migration, and invasion of tumor cells in vitro . (researchsquare.com)
  • This study observes a previously neglected pharmacological phenomenon and investigates its mechanism of that the continuous low-dose administration of some antineoplastic agents in certain dose ranges can promote tumorigenesis and tumor progression in vitro and in vivo , through stimulation of tumor cell functions directly as well as enhancement of tumor angiogenesis by BMDCs recruitment indirectly. (researchsquare.com)
Migration12
  • It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
  • BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
  • Knockdown of RPL5 significantly inhibited the proliferation and migration of HCT16 and RKO cells, and arrested the cell cycle in G0/G1 phase. (biomedcentral.com)
  • Moreover, TBHQ could partially reverse the inhibitory effect of siRPL5 on the proliferation and migration of colon cancer cells. (biomedcentral.com)
  • Collectively, RPL5 promoted colon cell proliferation and migration, at least in part, by activating the MAPK/ERK signaling pathway. (biomedcentral.com)
  • RPL5 promoted colon cell proliferation and migration, at least in part, by activating the MAPK/ERK signaling pathway, which may serve as a novel therapeutic target for cancers in which MAPK/ERK signaling is a dominant feature. (biomedcentral.com)
  • Here, we demonstrate that RPL5 promotes COAD cell proliferation and migration via the MAPK/ERK signaling pathway. (biomedcentral.com)
  • The migration was also impaired in FASN-silenced cells. (biomedcentral.com)
  • And the influence of FASN on proliferation and migration of colorectal cancer cells was also explored. (biomedcentral.com)
  • Effects of lncRNA overexpression or downregulation on cell proliferation, clonogenicity, migration and chemosensitivity were studied in HNSCC cell lines. (biomedcentral.com)
  • Unexpectedly, up- or downregulation of CASC9 expression in HNSCC cell lines with low or high CASC9 expression, respectively, did not result in significant changes of cell viability, clonogenicity, migration or chemosensitivity. (biomedcentral.com)
  • Metastatic recurrence is a leading cause of cancer-associated death, and cancer cell migration is the initial step among the metastatic process. (aging-us.com)
Knockdown5
  • In addition, SNHG4 or RRM2 knockdown significantly induced cell cycle arrest and cell senescence, and inhibited DNA damage repair and cell proliferation, and the effects can be partially reversed by let-7a knockdown or RRM2 reoverexpression. (biomedcentral.com)
  • The knockdown of ATG9B expression in GBM cells suppressed TMZ-induced autophagy as well as TMZ-resistance. (nature.com)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • Knockdown of TSPAN12 expression in A2780 and SKOV3 cells decreased both proliferation and colony formation. (molcells.org)
  • Knockdown of SETD8 increased the responsiveness of cervical cancer cells to cisplatin treatment. (biomedcentral.com)
Viability5
  • ACT‑3 significantly decreased the cell viability in a concentration‑dependent manner and induced different morphological changes at high concentrations. (iasp-pain.org)
  • AKT is a key molecule in the PI3K/AKT/mTOR signaling pathway, which has been shown to serve notable functions in the regulation of cell viability and to be closely associated with a variety of disorders caused by dysfunctional cellular proliferation ( 8 , 9 ). (spandidos-publications.com)
  • Here, we established Live-seq, a single-cell transcriptome profiling approach that preserves cell viability during RNA extraction using fluidic force microscopy2,3, thus allowing to couple a cell's ground-state transcriptome to its downstream molecular or phenotypic behaviour. (bvsalud.org)
  • This was performed by measuring cell growth, viability, clonogenic activity, cell cycle and reactive oxygen species (ROS) generation. (utmb.edu)
  • The treatments of CE and COC resulted in the reduction of the growth fraction of alveolar epithelial type II cells without significant decrease in viability. (utmb.edu)
Squamous cell carc7
  • At present, advanced stage human Papillomavirus (HPV) negative and positive head and neck squamous cell carcinoma (HNSCC) are treated by intense multimodal therapy that includes radiochemotherapy, which are associated with relevant side effects. (oncotarget.com)
  • Two main etiologies of head and neck squamous cell carcinoma (HNSCC) are recognized that reveal distinct biological characteristics. (oncotarget.com)
  • Thrombospondin type 1 domain-containing 7A ( THSD7A ) was reported to play a procancer role in esophageal squamous cell carcinoma (ESCC). (hindawi.com)
  • Therefore, it is important to explore candidate genes that could have significance to the clinical diagnosis, as well as the treatment of esophageal squamous cell carcinoma (ESCC) at the molecular level [ 4 ]. (hindawi.com)
  • GPC3 expression is significantly higher in LUSC (lung squamous cell carcinoma) than in lung adenocarcinoma. (biolifesas.org)
  • Few diagnostic and prognostic biomarkers are available for head-and-neck squamous cell carcinoma (HNSCC). (biomedcentral.com)
  • Analysis of TCGA pan-cancer data revealed significant overexpression of CASC9 across different other entities including bladder, liver, lung and stomach cancers and especially in squamous cell carcinoma (SCC) of the lung. (biomedcentral.com)
Transwell2
  • The MTT, colony formation, flow cytometry, wound healing, transwell, and western blotting were performed to examine the function of miRNA-149 and FOXC1 in the development of gastric cancer. (hindawi.com)
  • Materials and Methods: The suppression effects of propofol on the proliferation and invasion of cervical cancer cells were analyzed by Cell Counting Kit-8 (CCK-8), colony formation and Transwell invasion assay. (aging-us.com)
Overexpression4
  • Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
  • In this study, the authors used DNA microarray to compare and identify genes induced by HER2 in mammary epithelial cell line with ectopic HER2 overexpression and breast cancer cell lines derived from patients with different level of HER2 expression. (biomedcentral.com)
  • Further exploration showed that TSPAN12 overexpression accelerated proliferation and colony formation of OVCAR3 and SKOV3 OC cells. (molcells.org)
  • GPC3 overexpression can accelerate the growth of lung squamous cells and the occurrence of LUSC, highlighting its role in LUSC progression. (biolifesas.org)
Differentiation10
  • As a proof of concept, we show that Live-seq can be used to directly map a cell's trajectory by sequentially profiling the transcriptomes of individual macrophages before and after lipopolysaccharide (LPS) stimulation, and of adipose stromal cells pre- and post-differentiation. (bvsalud.org)
  • We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
  • Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • The SRY-box 4 (SOX4) gene, which is located on chromosome 6p22.3, encodes a 47-kDa protein that is a member of the sex-determining region Y-related high-mobility group-box transcription factor family and has functions in embryonic development and cell differentiation ( 3 , 4 ). (spandidos-publications.com)
  • Moreover, it could affect the internal m6A modification of lincRNA1281 and mediate a competing endogenous RNA (ceRNA) model to regulate mouse embryonic stem cell differentiation [ 30 ].Alarcon et al found that METTL3 could promote the maturation of miRNAs (let-7e, miR221/222, miR4485, miR25, miR93, miR126 and miR335 et al) by interact with the microprocessor protein DGCR8 [ 24 ]. (biomedcentral.com)
  • Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
  • This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
  • These cells possess both differentiation plasticity (stemness) and tissue supportive functions (stromalness) that can coexist and overlap, with differences depending on tissue source, donor characteristics, culture conditions and delivery strategies, leading to alternative best fittings for the term "stem" or "stromal" [ 7 ] . (encyclopedia.pub)
  • However, type II cells have several important functions, including the production of surfactant, ion transport, and proliferation and differentiation into type l cells after cellular injury. (medscape.com)
Invasion8
  • A minor subpopulation of cancer cells, known as glioma stem-like cells (GSCs), are thought to play a major role in tumor relapse due to their stem cell-like properties, their high resistance to conventional treatments and their high invasion capacity. (nature.com)
  • SCARA5 expression is significantly downregulated in various tumor tissues including liver cancer (LC), rectal cancer, and glioblastoma [ 15 , 16 ] and might affect cell proliferation and invasion abilities by the focal adhesion kinase (FAK) signaling pathway and epithelial-mesenchymal transition (EMT) [ 5 , 17 - 20 ]. (hindawi.com)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • This study was intended to identify the function of long non-coding RNA (lncRNA) lncARSR in NAFLD and its role in human HCC cells (HepG2) proliferation and invasion. (biomedcentral.com)
  • LncARSR activated the IRS2/AKT pathway by reducing YAP1 phosphorylation, and further increased lipid accumulation, cell proliferation, invasion and cell cycle. (biomedcentral.com)
  • Silencing lncARSR suppressed the IRS2/AKT pathway, consequently reducing HCC cell proliferation and invasion and inhibiting lipid accumulation in NAFLD mice by downregulating YAP1, which suggests a clinical application in treating NAFLD. (biomedcentral.com)
  • Du X , Wang X , Zheng Y , Liu D , . Propofol suppresses the growth and invasion of cervical carcinoma cells by inhibiting MIR155HG. (aging-us.com)
  • Conclusions: Altogether, these results indicated that propofol restrained the growth and invasion of cervical cancer cells partly via regulating MIR155HG expression. (aging-us.com)
Replication9
Hematopoietic stem8
Genes4
  • Expression of YAP1, adipogenesis- (Fasn, Scd1 and GPA) and IRS2/AKT pathway-related genes was measured. (biomedcentral.com)
  • Background: HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. (123dok.net)
  • The phage remains in a lysogenic state until environmental conditions induce expression of phage lytic cycle genes, leading to new phage production and lysis of the host bacterium. (cdc.gov)
  • therefore, induction of the lytic cycle increases expression of the toxin genes and host cell lysis allows for toxin release ( 10 ). (cdc.gov)
Tissues and cell lines3
  • The expression level of RPL5 in colon cancer tissues and cell lines was significantly higher than that in adjacent tissues and NCM460 cells, respectively, and its expression level was higher in HCT116 cells and RKO cells. (biomedcentral.com)
  • In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
  • BCRC-3 was lowly expressed in bladder cancer tissues and cell lines. (biomedcentral.com)
Inhibits4
  • A downregulation of FASN effectively inhibits the activity of "HER2-PI3K/Akt axis" and alters the malignant phenotype in colorectal cancer cells. (biomedcentral.com)
  • PGM1 inhibits hepatocellular carcinoma cell (HCC) proliferation and growth by utilizing sufficient extracellular glucose to convert glycogen, while deletion of the PGM1 gene inhibits glycogen synthesis and leads to glycolysis of additional glucose, thus promoting tumor cell proliferation and growth [ 15 ]. (biomedcentral.com)
  • FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
  • In colorectal cancer, propofol inhibits cancer cells proliferation and metastasis by regulating miR-124-3p.1/AKT3 [ 13 ]. (aging-us.com)
Metastasis2
  • The anti-cancer impact of propofol on cervical cancer cells growth in vivo was determined by means of xenograft tumor model and lung metastasis model. (aging-us.com)
  • Prior studies have suggested that propofol (2, 6-diisopropylphenol), one of the most extensively used intravenous anesthetic agents during cancer surgical excision, influences the biological behaviors of cancer cell, including growth, invasiveness, and metastasis [ 10 , 11 ]. (aging-us.com)
Regulation8
  • Furthermore, the data of the present study indicated that B‑cell lymphoma 2 and cyclin D1 is involved in the regulation of AKT1 expression. (spandidos-publications.com)
  • Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
  • Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
  • Cyclin A2, Cyclin D1, Cyclin E2, CDK2, and CDK4) were significantly involved in the regulation of cell cycle downstream of TSPAN12. (molcells.org)
  • It affects the tumor formation by the regulation the m6A modification in the mRNAs of critical oncogenes or tumor suppressors. (biomedcentral.com)
  • To our knowledge, this is the first comprehensive study that METTL3 affected the tumor formation by the regulation the m6A modification in non-coding RNAs, which might provide fresh insights into bladder cancer therapy. (biomedcentral.com)
  • In this study, we aimed to explore the potential involvement of Gas5 in papillary thyroid carcinogenesis and to highlight the emerging roles of ceRNAs in the biological regulation of PTC cells. (oncotarget.com)
  • Other trophic factors, such as IL-2, TNF-α, and granulocyte-macrophage stimulating factor, cause activated T cells to produce interferon-γ, TNF-α, and TNF-β, leading to stimulation of macrophages and further up-regulation of leukocyte adhesion molecules. (medscape.com)
SiRNA4
  • Based on high-resolution microscopic images, high content analysis (HCA) enables you to detect cellular phenotypic changes at a molecular level, so the technology can be utilized as a powerful tool to study effects of physiologically active substances, such as compounds, siRNA, peptides and antibodies, on cultured cells, tissue samples and whole organisms, like zebrafish. (yokogawa.com)
  • Interestingly, targeting WDR5 by siRNA and OICR-9429 could block IFN-γ-induced PD-L1 expression in PCa cells. (thno.org)
  • Contrary to cells treated with a siRNA, cells treated with siRNAs against Bora often displayed multipolar spindles in mitosis, a phenotype that is also seen upon TPX2 RNAi Geneticin distributor and after injection of antibodies blocking Aurora A function. (others-signal.com)
  • HOXD9 siRNA treatment of SK-MG-1 SP cells resulted in reduced cell proliferation. (123dok.net)
Tumors6
  • Since then they were systematically cultured as tumorospheres in defined stem cell culture conditions, allowing them to keep their GSC properties including their capacity to generate intracerebral tumors in immunodeficient mice (Supplementary Fig. S1 A). (nature.com)
  • SOX4 has been revealed to induce EMT, a key process during organ development and the progression of epithelial tumors to metastatic cancers ( 9 ), via its activation of the Wnt and transforming growth factor β signaling pathways in cancer cells ( 10 , 11 ). (spandidos-publications.com)
  • The novel PLK1 inhibitor, onvansertib, is the first available for oral administration and has shown tumor growth inhibition in hematologic tumors, osteosarcoma, ovarian carcinoma, breast cancer and colon adenocarcinoma cells. (childrenscolorado.org)
  • Overall, these results suggest that tumors are unlikely to arise directly from BRCA2 heterozygous cells without other genetic events such as loss of the wild-type BRCA2 allele and/or loss of p53 function or other cell cycle inhibitors. (ijbs.com)
  • Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
  • At the end of the experiment, the mice were weighed and their tumors were measured. (cab-aurel.com)
Progenitor6
  • Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
  • The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
  • When stimulated to divide, they give rise to more committed, differentiated daughter cells with less proliferative potential, called "early progenitor" cells. (justia.com)
  • Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
  • In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
  • Finally, we cultured human glioma cancer stem cells (GCSCs) from patient specimens found with high expression of HOXD9 in GCSCs compared with normal astrocyte cells and neural stem/progenitor cells (NSPCs). (123dok.net)
Colorectal cancer1
  • Hence, in this study, we analyzed the potential link between FASN and the activity of "HER2-PI3K/Akt axis" in colorectal cancer cells. (biomedcentral.com)
Epithelial cell line1
  • We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
Inhibition3
  • Anticancer effects of the HDAC inhibitor, 3β,6β‑dihydroxyurs‑12‑en‑27‑oic acid, in MCF‑7 breast cancer cells via the inhibition of Akt/mTOR pathways. (iasp-pain.org)
  • In all five HPV negative and HPV positive cell lines tested, roscovitine caused inhibition of CDK1 and 2. (oncotarget.com)
  • The exposure of C. neoformans cells or preformed cryptococcal biofilms to fluconazole or voriconazole did not result in yeast growth inhibition and did not affect the metabolic activities of the biofilms, respectively. (who.int)
Pathway4
  • Additionally, ACT‑3 significantly increased autophagic cell death by inhibiting the serine‑threonine kinase/mammalian target of the rapamycin pathway. (iasp-pain.org)
  • In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
  • A transcriptome analysis of HER2 in breast cancer cells has revealed a molecular connection between FASN and HER2 through the PI3K/Akt pathway [ 10 ]. (biomedcentral.com)
  • Taken together, our study identified a tumor suppressive role of Gas5 in PTC cells acting as a ceRNA, effectively becoming a sink for miR-222-3p, modulating the expression of PTEN, which lead to PTEN/AKT pathway activation and proliferation suppression. (oncotarget.com)
Cellular7
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Single-cell transcriptomics (scRNA-seq) has greatly advanced our ability to characterize cellular heterogeneity1. (bvsalud.org)
  • To benchmark Live-seq, we used cell growth, functional responses and whole-cell transcriptome read-outs to demonstrate that Live-seq can accurately stratify diverse cell types and states without inducing major cellular perturbations. (bvsalud.org)
  • Thses days, using 3D samples such as spheroids and organoids, and live cell samples to mimic realistic cellular environment are becoming more and more popular due to cell culture technology development. (yokogawa.com)
  • The characterization of the resulting cells with regard to key cellular BRCA2 functions is presented here. (ijbs.com)
  • At the cellular level, aging is marked by depletion of adult stem cell reservoirs, the inability to maintain baseline homeostasis, a reduced response to stress, an increased accumulation of damaged DNA leading to telomere shortening, and mitochondrial dysfunction [ 1,2 ]. (karger.com)
  • The scientists investigated the cellular effects of FZ by using a clonogenic assay, in which H460 cells were treated with 1 uM FZ for 48 hours and their colony formation was assessed after Hoechst 33342 staining (i & ii) or TdT staining (iv & v). In this test, both free FZ and the FEN/RAPA-loaded micelles effectively inhibited cancer cell proliferation at concentrations up to 2090 nM. (cab-aurel.com)
Induce1
  • Hence, it is of great importance to investigate the mechanisms that induce enzalutamide resistance in prostate cancer cells. (biomedcentral.com)
Promotes3
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
  • Injury to the alveolar lining cells also promotes pulmonary edema formation. (medscape.com)
Progression3
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • Moreover, TSPAN12 accelerated mitotic progression by controlling cell cycle. (molcells.org)
  • Cell cycle progression was also affected. (frontiersin.org)
Alveolar Epithelial Cells2
Transcription3
Bone marrow5
  • Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
  • These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
  • After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
  • The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
  • Due to the low abundance of MSCs in human adult tissues (about 1/10 6 cells in adult bone marrow and 1/10 3-4 cells in adipose tissue and umbilical cord) [ 16 ] , frequently ex-vivo expansion precedes therapeutic administration, to obtain a clinically relevant number of cells. (encyclopedia.pub)
Pathways2
  • In this review, we examine aging as a process dependent on specific changes in molecular pathways within multiple lung cell populations. (karger.com)
  • There are multiple types of cell death, several interconnected signaling pathways can be activated, and the postmortem consequences may vary. (cshlpress.com)
Mesenchymal3
  • Mesenchymal stem/stromal cells (MSCs) are a reservoir for tissue homeostasis and repair that age during organismal aging. (encyclopedia.pub)
  • While much work has focused on the failure of epithelial cell populations as a key component of the aging process, additional studies have shown that aging, as a global phenomenon in the lung, also impacts resident endothelial, mesenchymal, and immune cell populations. (karger.com)
  • A growing body of evidence has identified that epithelial-mesenchymal transition (EMT) plays pivotal parts in cancer cell metastatic dissemination via endowing them with a more migrate and invasive trait [ 4 ]. (aging-us.com)
Somatic cells1
  • MSCs represent a lifelong reservoir for the generation of somatic cells and for cell replacement. (encyclopedia.pub)
Molecular2
  • However, scRNA-seq requires lysing cells, which impedes further molecular or functional analyses on the same cells. (bvsalud.org)
  • Subsequently, the effect of an additional small molecular inhibitor, BMS-777607 (which targets MST1R (RON), MET, Tyro3, and Axl) also resulted in a decreased proliferative capacity of MPM cells. (frontiersin.org)
Endothelial1
  • They are expressed on endothelial cells and bind to the integrins CD 11a/CD 18 (lymphocyte function-associated antigen-1) and VLA-4, respectively. (medscape.com)
Cervical cancer cells3
  • Propofol treatment decreased the expression of MIR155HG in cervical cancer cells. (aging-us.com)
  • Consistently, the results from in vivo xenograft model indicated that propofol repressed cervical cancer cells growth and decreased the expression of MIR155HG in vivo . (aging-us.com)
  • Furthermore, reintroduction of MIR155HG into cervical cancer cells counteracted the inhibitory potency of propofol on the growth and aggressive phenotypes in cervical carcinoma cells. (aging-us.com)
Flow cytometry analysis1
  • In the present study, the effects of ACT‑3 on anticancer effects of MCF‑7 cells were determined by measuring the level of apoptotic cell death and cell cycle regulator using flow cytometry analysis and western blot analysis, respectively. (iasp-pain.org)
Cancer cell proliferation1
Lines15
  • The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)
  • Twenty-four hours after plating on laminin substrate, TG1N and TG16 cells adopted a bipolar and elongated shape (Supplementary Fig. 1 B) and displayed high motility (mean velocities of 26.3 ± 0.6 µm/h and 25.7 ± 1.1 µm/h, respectively) without a predefined direction (Supplementary Fig. S1 C, Supplementary Movies S1 and S2 ), consistently with random motility pattern with high velocity previously reported for other GSC lines 30 . (nature.com)
  • Here we tested whether roscovitine, an inhibitor of cyclin-dependent kinases (CDKs), which hereby also blocks homologous recombination (HR), can be used to enhance the radiation sensitivity of HNSCC cell lines. (oncotarget.com)
  • Surprisingly, all HPV positive cell lines were found to be defective in HR. In contrast, HPV negative strains demonstrated efficient HR, which was completely suppressed by roscovitine. (oncotarget.com)
  • In line with this, for HPV negative but not for HPV positive cell lines, treatment with roscovitine resulted in a pronounced enhancement of the radiation-induced G2 arrest as well as a significant increase in radiosensitivity. (oncotarget.com)
  • Due to a defect in HR, all HPV positive cell lines were efficiently radiosensitized by the PARP-1 inhibitor olaparib. (oncotarget.com)
  • In contrast, in HPV negative cell lines a significant radiosensitization by olaparib was only achieved when combined with roscovitine. (oncotarget.com)
  • Compound 3a exhibited strong anti-proliferative activity against three cell lines: HepG-2 (IC50 14.31 ± 0.83 µM), A-549 (IC50 30.74 ± 0.76 µM), and MCF-7 (IC50 27.14 ± 1.91 µM). (bvsalud.org)
  • Also, it was four times more selectively cytotoxic against WI-38 cell lines than doxorubicin. (bvsalud.org)
  • Lentiviral and non-viral vector options allow the transfection or transduction of shRNA into difficult-to-transfect cells as well as more conventional cell lines. (genecopoeia.com)
  • An investigation of PLK1 expression in DepMap found PLK1 is a critical mediator in 55 central nervous system cell lines, including eight medulloblastoma cell lines. (childrenscolorado.org)
  • No difference in RAD51 subcellular localization and focus formation was observed between control and mimicked heterozygous cell lines. (ijbs.com)
  • Results: In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. (123dok.net)
  • The results suggested that Gas5 was markedly downregulated in both PTC tissues and PTC cell lines. (oncotarget.com)
  • 1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
Survival and proliferation1
  • The purpose of the present study was to reveal the effects of AKT1 on the survival and proliferation of HCC cells, and to investigate the mechanisms involved. (spandidos-publications.com)
Stem cell7
  • During aging of the organism, MSCs also age, and this implies an impairment of stem cell functions contributing to the progressive decrease in tissue maintenance and repair, a characteristic of the aging process. (encyclopedia.pub)
  • Actually, stem cell exhaustion is considered one of the promoters of aging. (encyclopedia.pub)
  • Lung failure due to aging can be traced to loss of lung stem cell regenerative capacity within the distinctive stem cell niches found within each compartment of the lung. (karger.com)
  • Current knowledge about the identity and function of these stem cell compartments has been largely drawn from a variety of transgenic and spontaneously mutated mouse models that are characterized by rapid rates of aging or have been used to examine regeneration from injury in the context of natural or accelerated aging. (karger.com)
  • In this review, we will present current data on lung aging and lung regeneration, with specific attention paid to stem cell-driven regeneration within the context of aging. (karger.com)
  • Stem cells are characterized by their ability to undergo self-renewal to maintain stem cell reserves, and, when required, to produce new, terminally differentiated cells. (karger.com)
  • During aging, a decline in organ function can be traced to a loss of stem cell function due to increased cell turnover, depletion of stem cells, and alterations to the stem cell niche. (karger.com)
Apoptotic cells1
  • The cell cycle was halted at the G1-S phase, and total apoptotic cells were 65 times more than control Hep-G2 cells. (bvsalud.org)
Regulates1
  • Low shear stress up-regulates expression of vascular cell adhesion molecule 1 (VCAM-1), while increased shear stress can lead to increased gene expression of intercellular adhesion molecule 1 (ICAM-1). (medscape.com)
Vivo xenograft1
  • The tumor weight and volume were then measured through in vivo xenograft tumor model after silencing lncARSR. (biomedcentral.com)
Growth8
  • Primig, M.: Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development. (tu-dresden.de)
  • Cancer cells obtain a growth advantage through uncontrolled cell proliferation ( Hanahan and Weinberg, 2011 ), which may be caused by mutations that help them adapt to the microenvironment through selective pressure. (molcells.org)
  • The oncogenic kinase PLK1 controls cell cycle and growth and is a cancer therapeutic target. (childrenscolorado.org)
  • Interestingly, the growth rate of the mimicked BRCA2 heterozygous cell line was significantly lower than that of control cells. (ijbs.com)
  • Levels of p27 were also found to be slightly increased in cells with reduced BRCA2, perhaps contributing to the slower growth rate. (ijbs.com)
  • However, it may be possible that tumor growth can be initiated in heterozygous cells prior to loss of the second, wild-type allele. (ijbs.com)
  • For example, propofol restrains pancreatic cancer cells growth and metastases via elevating miR-328 and depressing ADAM Metallopeptidase Domain 8 (ADAM8) [ 12 ]. (aging-us.com)
  • The results showed that tumor formation and growth were both significantly promoted by GEM or GEM+CDDP at as low as half of the metronomic dosages, which were accompanied by enhancements of angiogenesis in tumor tissues and the release of proangiogenic BMDCs in the circulating blood. (researchsquare.com)
Ultraviolet radiation1
  • DNA repair ability, as measured by colony survival following mitomycin C treatment and ultraviolet radiation exposure, was also unaffected by reduced levels of BRCA2. (ijbs.com)
Transcriptome1
Conclusions1
  • Conclusions: Our results suggest that HOXD9 may be a novel marker of GCSCs and cell proliferation and/or survival factor in gliomas and glioma cancer stem-like cells, and a potential therapeutic target. (123dok.net)