• A practical approach utilized in this study involves the preparation of an antibacterial thin film on the surfaces, which can effectively inhibit and reduce biofilm formation and bacterial adherence. (nature.com)
  • In this study, we examined the efficacy of silver zeolite for preventing biofilm formation by common foodborne pathogens such as Listeria innocua Seeliger and Escherichia coli O157:H7. (wur.nl)
  • Biofilm formation was assessed by cresyl violet assay, quantification of colony-forming units and scanning electron microscopy, and atomic force microscopy. (wur.nl)
  • The results of this study demonstrate that the silver zeolite could inhibit foodborne pathogenic biofilm formation and could potentially serve as an effective antimicrobial coating for food contact surfaces. (wur.nl)
  • Flavopiridol also exhibited antifungal activity by restricting fungal growth and limiting fungal biofilm formation and conidial adhesion. (researchsquare.com)
  • Notably, in contrast with the WT, which aggregates and forms biofilm, the aceE -mut lost its ability of growing aggregately and biofilm formation, which are two very important features of mycobacteria. (biomedcentral.com)
  • Over all, this study indicates that aceE gene plays a significant role in the mycolic acid synthesis and affects the colony morphology, biofilm formation of M. smegmatis and bacteria invasion of macrophage. (biomedcentral.com)
  • This mutant had obvious differences in colony morphology (smaller plaque, edge smooth and round) and defects in biofilm formation in contrast with the wild-type (WT) strains. (biomedcentral.com)
  • If the native host bacteria win the race, tissue cells will not be able to displace these primary colonizers, and biofilm formation will occur developing into peri-implantitis [ 4 , 5 ]. (hindawi.com)
  • The role of bacterial communication, also known as quorum sensing is an important mechanism in biofilm formation which is fundamental to the development of anti-biofilm strategies. (westminster.ac.uk)
  • In this current study, the synergy between a quorum sensing inhibitor (cinnamaldehyde) and two antibiotics (ceftazidime and levofloxacin) was evaluated in an attempt to develop a strategy for biofilm disruption using the high-throughput minimum biofilm eliminating concentration (MBEC) assay. (westminster.ac.uk)
  • The disk diffusion method was applied to determine antimicrobial resistance patterns according to the Clinical and Laboratory Standards Institute, and the microtiter plate assay was managed to explore the biofilm formation. (japsonline.com)
  • The isolates were identified by Vitek-2 and characterized based on growth kinetics, biofilm formation, motility, pyoverdine and pyocyanin production, phospholipase and catalase activity, urease production along with expression of exotoxins (exo-A, exo-U and exo-S) and correlated to its antibiotic profiles. (springeropen.com)
  • The aim of our present study was to screen P. aeruginosa strains isolated from ocular infections, for their potential to produce various phenotypic virulence factors and correlating them with biofilm formation and their antibiotic susceptibility profile. (springeropen.com)
  • this was also supported by a reduction in biofilm formation of both bacteria. (biomedcentral.com)
  • The formation of bacterial biofilm on construction material plays a key role in the possible occurrence of pathogen infections in nosocomial environments as well as in building biodeterioration [ 7 , 8 ]. (biomedcentral.com)
  • Cellulose membrane disks (13 mm, N=12) were used for biofilm formation (24 h) of S. mutans and L. casei , which were treated (1 min) with 0.4% RPE or 0.12% Chlorhexidine (CHX). (bvsalud.org)
  • The control group of biofilm formation was not submitted to any treatment. (bvsalud.org)
  • Materials and method: samples of acrylic and silicone based materials were submitted to biofilm formation using 24- well polystyrene cell tissue culture plates to form C. albicans biofilm for 1, 7 and 14 days. (bvsalud.org)
  • Brushing alone could be insufficient for controlling biofilm formation in geriatric patients, thus chemical denture cleansers should be used to achieve suitable hygiene 3 . (bvsalud.org)
  • Stimulation of granulopoiesis by transforming growth factor beta: synergy with granulocyte/macrophage-colony-stimulating factor. (ox.ac.uk)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • and today is the most widely used in vitro assay for HSPCs. (stemcell.com)
  • Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
  • To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
  • The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)
  • In the anticoagulative assay, compounds 1 and 2 exhibited weak antithrombin activity and strong antiplatelet aggregation activity in vitro. (alexbotsaris.com.br)
  • Furthermore, the antimicrobial abilities evaluated in vitro with Streptococcus mutans ( S. mutans ), Fusobacterium nucleatum ( F. nucleatum ) and Candida albicans ( C. albicans ) using the live/dead, MTT and colony-forming units (CFUs) assay. (peerj.com)
  • In vitro assays that permit cloning of tumour cells in soft agar have been improved during the last 5 years. (tno.nl)
  • Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. (abmgood.com)
  • The underlying mechanisms involved in genomic stability, apoptosis and oxidative stress-related signaling were further analyzed by Western blotting, flow cytometry and immunofluorescence assays. (biomedcentral.com)
  • Moreover, treatment with rhGH increased the frequency of hematopoietic stem/progenitor cells as measured by flow cytometry and colony forming unit assays in bone marrow harvested at day 14 after irradiation, suggesting the effects of rhGH are at the hematopoietic stem/progenitor level. (duke.edu)
  • The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
  • MSCs were isolated from PB by direct plating in a tissue culture medium, and cell proliferation (population doubling time [PDT], and colony-forming unit-fibroblast assay [CFU-F]), and characterization (morphology, plastic adhesiveness, colony formation, trilineage differentiation) were examined. (e-jvc.org)
  • At 10 weeks, Fe2O3-exposed cells displayed significant enhanced proliferation, invasion, soft agar colony formation and colony forming unit ability suggesting a neoplastic-like transformation. (cdc.gov)
  • MWCNT-exposed cells exhibited increased colony formation ability while nCeO2 was negative in all assays except proliferation. (cdc.gov)
  • Wound-healing assay and colony formation analysis were employed for migration and proliferation evaluation. (jmbfs.org)
  • PB-MSCs from both mares and foals showed typical MSC characteristics in terms of spindle-shaped morphology, plastic adhesive properties, formation of colonies, trilineage differentiation. (e-jvc.org)
  • Our results now suggest that 4-MU treatment of MSC not only affects the formation of SG via increased levels of O-linked glycosylation of cellular proteins but also that MSC cultivated under osteogenic stimulation develop SG, which in turn support MSC differentiation into osteogenic precursor cells. (europa.eu)
  • By day 2 from the time of plating, each CFU-E colony will contain between 8 (minimum) and 64 hemoglobinized cells most of which are in their end-stage of erythroid differentiation. (wikipedia.org)
  • Likewise, an earlier stage of erythroid differentiation may also yield colonies in Epo-only medium, but these colonies would likely be smaller and/or not hemoglobinized, since the stages before the CFU-E stage (MEP and BFU-E) require other factors (IL-3 etc) and more time for growth that will also delay the terminal differentiation and hemoglobinization. (wikipedia.org)
  • This property forms the basis of in vivo assays of HSC function. (stemcell.com)
  • We hypothesized that changes in morphology may indicate changes in cell wall metabolism and identified an aceE gene mutant ( aceE -mut) which presented a deficient colony morphology on 7H10 agar by screening transposon mutagenesis in Mycolicibacterium smegmatis , basonym Mycobacterium smegmatis ( M. smegmatis ). (biomedcentral.com)
  • We observed that the colony morphology of aceE -mut was quite different, smaller and smoother on the solid culture medium than the wild-type (WT) strain during the transposon library screening of M. smegmatis . (biomedcentral.com)
  • Morphology - colony formation - staining - aging - physiological comparison, with acute leukemia marrow fat cells. (bremel.gr)
  • The useful capability of EPCs was determined by colony forming unit (CFU) assay and endothelial tube formation assay. (ecolowood.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Investigation of toxicity of Cu-Ti thin films was conducted the using the MTT assay, which revealed their biocompatibility and absence of cytotoxicity, further confirming their potential as promising biomaterials for various applications. (nature.com)
  • The MTT and LDH assays were performed to evaluate the cytotoxicity effect of the extract and the determination of cell death. (jmbfs.org)
  • These strains produced and released Stx1a as measured by cytotoxicity and neutralization assays using anti-Stx/Stx1a antiserum. (cdc.gov)
  • Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
  • MegaCult™-C Collagen and Medium with Lipids kit includes medium and collagen solution for colony-forming unit (CFU) assays of human or mouse megakaryocyte progenitor cells (CFU-Mk). (stemcell.com)
  • Burst forming unit-erythroid colony formation inhibition assay suggesting possible erythroid suppression mechanism. (bvsalud.org)
  • CFU-E stands for Colony Forming Unit-Erythroid. (wikipedia.org)
  • It arises from CFU-GEMM (via BFU-E, which stands for "erythroid burst-forming units") and gives rise to proerythroblasts. (wikipedia.org)
  • CFU-E colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue (bone marrow, spleen, or fetal liver), which may be reflective of the organism's demand for oxygen delivery to the tissues or a hematopoietic disorder. (wikipedia.org)
  • Next, Fe2O3 and MWCNT soft agar colonies were isolated and placed in culture to evaluate persistence and longevity of the transformed phenotype. (cdc.gov)
  • Either mechanism is different from the widely held notion that most cells in a tumor should be competent for tumor formation. (aacrjournals.org)
  • Colony-forming unit assays yielded denser cell colonies in the HA treated samples compared to the controls, yet no difference in the number of colonies was detected, indicating that the number of stem cells remained unchanged. (europa.eu)
  • Phagocytosis of RAW 264.7 cells was evaluated by counting colony forming units. (researchsquare.com)
  • It is possible to see a small spectrum of hemoglobinization level and possibly cell size, indicating that some cells in the colony have achieved the end-stage faster than others. (wikipedia.org)
  • Cell number in a colony is important because pro-erythroblast stage is also Epo-responsive (expresses Epo receptor), but the proliferative capacity of these cells is not as high, thus yielding a colony with fewer than 8 cells. (wikipedia.org)
  • 03:33 the only cells in that colony. (yale.edu)
  • The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. (nature.com)
  • It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)
  • Antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. (tno.nl)
  • The experiments presented here suggest that the adherent cells involved in this phenomenon are the progeny of bone marrow derived fibroblastoid colony forming units. (tno.nl)
  • Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • Colony-forming unit assay To examine the effect of the colony-forming ability, EPCs were cultured in MethoCult? (biobender.com)
  • Absorbance assay, crystal violet staining and adherence assay were used to detect the antifungal activity of flavopiridol. (researchsquare.com)
  • Klebsiella pneumoniae and Proteus mirabilis biofilms of initial broth suspensions of 108 colony forming units (CFU) per mL, cultivated on the pegs of the MBEC device were challenged with 5120 µg/ml of ceftazidime and levofloxacin in a double dilution assay in the presence of 500 µM cinnamaldehyde. (westminster.ac.uk)
  • Antibiofilm activity was examined based on the formation and disruption of bacterial biofilms and evidenced by the crystal violet technique. (brieflands.com)
  • The essential oil also showed an important inhibitory effect against the formation of biofilms produced by Staphylococcus aureus , since it inhibited the formation of biofilms by over 50% at the concentration of 64 µg mL -1 . (brieflands.com)
  • Antibacterial activity of the prepared sample was assessed by determining the number of colony-forming units per milliliter (CFU/ml) using a standard viable cell count assay. (nature.com)
  • A Cell Counting Kit-8 assay was used to test cell viability. (researchsquare.com)
  • These collectively form a thick, dense, poorly permeable cell wall, which not only allows mycobacteria to resist the dry environment and harmful chemicals but also allows it to reproduce in the macrophages [ 4 , 6 ]. (biomedcentral.com)
  • Besides, EA extract significantly inhibited cell migration and colony formation at 250 to 1000 µg/ml concentration. (jmbfs.org)
  • A single-cell assay identified a progenitor cell that directly responded to TGF-beta and GM-CSF. (ox.ac.uk)
  • the progenitor cell is tentatively designated granulocyte burst-forming unit. (ox.ac.uk)
  • With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
  • Depending on the dosage, the formation of openings in the cell membranes caused by the electric pulses may or may not be reversible. (bvsalud.org)
  • The length of tubuli in a Vicriviroc maleate matrigel assay with HUVECs decreased significantly in response to pCRP but not to mCRP. (ecolowood.com)
  • Formation stages - staining - fibroblast interference - lipogenesis. (bremel.gr)
  • Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. (abmgood.com)
  • The concentration for all three types of viruses can also be classified as IU/ml (Infectious Units/ml). (abmgood.com)
  • In this regard, radiation therapy and accidental exposure to gamma (γ)-ray radiation have been shown to inhibit bone formation and increase the risk of fractures. (biomedcentral.com)
  • Enzyme assays confirm that PTC-847 and PTC-672 inhibit Ng RNR and reveal that allosteric effector dATP potentiates the inhibitory effect. (elifesciences.org)
  • Quantitative real-time PCR assays were carried out with Applied Biosystems 7500 real-time PCR system using SYBR? (ecolowood.com)
  • In this work, we have tested the human canonical members of the RNase family using a spot-culture growth inhibition assay based mycobacteria-infected macrophage model for evaluating their anti-tubercular properties. (frontiersin.org)
  • Formation - maturation - mixed - megakaryocytes in CaS - CA from normal marrow - CA from myelodysplastic syndrome. (bremel.gr)
  • Morphological examination showed an increase in granulocyte colonies. (ox.ac.uk)
  • Methylcellulose is a semisolid media additive that allows an investigator to stain (with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor that is at the CFU-E stage. (wikipedia.org)
  • Counts of Candida were calculated and the results expressed in colony forming units (CFU)/ mm2. (bvsalud.org)
  • Anodization leads to an oxidation of metal species that form a solid oxide on the metal surface. (hindawi.com)
  • An ideal transmucosal implant surface exposed to the oral cavity is recommended to be highly polished to resist bacterial colonization and it is expected to allow the formation of an epithelial seal that prevents plaque accumulation leading to peri-implantitis [ 5 , 6 ]. (hindawi.com)
  • A significant difference was observed in bacterial composition between rooms cleaned with bleach and those cleaned with a quaternary ammonium compound (QAC) for composite 2 (overbed table, intravenous pole, and inner room door handle) (ANOSIM R2=0.66, p=0.005) but not composite 1 (bed rails, television remote control unit, call buttons, and telephone). (cdc.gov)
  • However, the identity of SSCs in young bone marrow and how they coordinate active bone formation remains unclear. (nature.com)
  • However, the identity of bone marrow SSCs during the transition, and how they contribute to active bone formation occurring in young stages remains unclear. (nature.com)
  • arrestin to modulate bone formation and bone resorption. (duke.edu)
  • Specific blockage of auto-phagosome formation and maturation reduced the protein's ability to eradicate the infection. (frontiersin.org)
  • It mimics the CF-lung environment during infection and causes typical P. aeruginosa phenotypes as mucoidy and microcolony formation [ 12 ]. (biomedcentral.com)
  • It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
  • Ordered nanotubular structures of TiO 2 , with a controlled and uniform diameter, length, and wall thickness, can be formed if the dissolution is enhanced by fluoride containing electrolytes and suitable anodization conditions are used [ 14 ]. (hindawi.com)
  • Fungal isolates that displayed the highest ratio of clear zone/colony diameter on plates of phosphate solubilization medium, were selected and identified as Aspergillus niger and Penicillium sp. (scialert.net)
  • According to the World Health Organization (WHO), TB is responsible for about 1.6 million TB deaths and 10 million (5.8 million men, 3.2 million women, and 1.0 million children) new cases have been detected in 2017 and more than a third of the world population is hosting Mycobacterium tuberculosis , the causative pathogen of TB, in its latent form ( 1 , 2 ). (frontiersin.org)
  • METHODOLOGY: A cross-sectional study was performed by means of direct observation involving 60 Intensive Care Units (ICU) and clinical nursing professionals in a Brazilian hospital. (bvsalud.org)
  • This study included 74 P. aeruginosa isolates recovered from 412 clinical isolates taken from patients urine admitted to wards/intensive care units (ICUs) and who acquired nosocomial infections after excluding signs or symptoms of infection at the time of admission. (japsonline.com)
  • Adenoviruses are measured as PFU/ml (plaque-forming units per millilitre). (abmgood.com)
  • Migration assay To examine the result of bisphosphonates on EPC migration, a 24-well Boyden chamber assay program (ThinCert? (biobender.com)
  • This method also used bead beating combined with the Maxwell 16 Casework kit for DNA extraction and purification and a real-time duplex PCR assay for toxin B and cdd3 genes to confirm the identity of the C. difficile spores. (cdc.gov)
  • Additional sex combs-like 1 ( ASXL1 ) mutations have been described in all forms of myeloid neoplasms including chronic myelomonocytic leukemia (CMML) and associated with inferior outcomes, yet the molecular pathogenesis of ASXL1 mutations ( ASXL1 -MT) remains poorly understood. (biomedcentral.com)
  • Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit (CFU-E)". Blood. (wikipedia.org)
  • Resistance mutations in Ng map to the N-terminal cone domain of the α subunit, which we show here is involved in forming an inhibited α 4 β 4 state in the presence of the β subunit and allosteric effector dATP. (elifesciences.org)