MacConkeyIncubationCultured on sheep blood agarSheep bloodHemolysis on blood agarTrypticase soyHemolyticBacteriaMorphologyNutrient agarMucoid coloniesCulturesColorlessRodsSterileSputumSelectiveComplete lysisStaphylococcusDiameterCorynebacteriumMannitolBrothBiochemicalSupernatantPlateOrganismCatalaseFungalLaboratoryInhibitoryCountsUrinePlatesGrowthRevealsStrainsCharacteristicsChocolatePathogensMediumPrimaryPeripheral bloodProteinColiHorse
MacConkey6
- Urine samples were cultured on MacConkey agar medium and blood agar medium. (banglajol.info)
- Sorbitol-MacConkey (SMAC) agar was developed to take advantage of this characteristic by substituting the carbohydrate sorbitol for lactose in MacConkey agar and is the medium of choice for isolation of E. coli O157:H7 (4). (cdc.gov)
- Examples include Escherichia coli, Klebsiella spp, Citrobacter, Enterobacter, etc.Pale (NLF) and pink (LF) colonies on MacConkey Agar. (3ia-technology.com)
- Sputum specimens were collected and inoculated into chocolate agar, blood agar, Manitol salt agar and MacConkey agar which then incubated at 35 OC or 37 OC for 24-48 hours. (alliedacademies.org)
- The sample was also inoculated onto a dip slide (Uricult® Duo, Orion Diagnostics, Finland), which contains an aselective cystine-lactose-electrolyte deficient (CLED) agar for Gram-positive bacteria and enterobacteriaceae , and MacConkey agar for non-glucose fermenting Gram-negative rods. (biomedcentral.com)
- nutrient agar and on MacConkey agar. (bvsalud.org)
Incubation4
- After 48 hours of incubation, a blood agar plate revealed growth of non-hemolytic, round, gray-to-white colonies measuring less than 1 mm in diameter. (asm.org)
- Incubation was repeated, colony-forming unit counts were performed, and statistical analysis was carried out using analysis of variance transforming results to Log 10 (x + 1), the highest interaction level was used to calculate standard error. (allenpress.com)
- Strains form small (c 0.5 mm in diameter) colonies on Columbia sheep blood agar after 5 days incubation [1]. (homd.org)
- Grows as 'breadcrumb' colonies on blood agar within 2-10 days' incubation. (medicofem.com)
Sheep blood4
- Mannitol-fermenting colonies (yellow or gold) were selected from the MSA plates and subcultured to trypticase soy agar + 5% sheep blood plates (BAP) and incubated at 37°C overnight. (cdc.gov)
- Hemolytic activity was observed on sheep blood agar. (magiran.com)
- This strain produces alpha hemolysis on sheep blood agar. (atcc.org)
- Actinobaculum schaalii grows slowly after 48 h in an anaerobic atmosphere at 37°C as tiny grey colonies, less than 1 mm in diameter, and shows weak β-hemolysis on agar plates containing 5% horse or sheep blood. (biomedcentral.com)
Hemolysis on blood agar2
Trypticase soy1
Hemolytic4
- Bacterial cultures of the blood are positive for a Gram-positive ꞵ-hemolytic cocci. (osmosis.org)
- The rough morphotype grew as equally white dry colonies which were hemolytic and had wrinkled edges. (microbiologyresearch.org)
- Colonies are golden and strongly hemolytic on blood agar. (medscape.com)
- Beta-hemolytic streptococci produce zones of clear hemolysis around each colony. (msdmanuals.com)
Bacteria6
- Blood agar culture with growing colonies of Corynebacterium diphtheria type (belfanti) bacteria. (cdc.gov)
- In comparison, MALDI-TOF-MS can identify bacteria and fungi within minutes directly from colonies grown on culture plates. (molvis.org)
- Throughout the cortex, there are multiple abscesses containing abundant colonies of bacteria that often involve glomeruli (arrow). (avma.org)
- A reproducible, preclinical model is essential to evaluate future methods used to mitigate blood-borne bacteria hardware and bone infections. (biomedcentral.com)
- Early onset colonization of an implant is often caused by introduction of bacteria into the surgical site during placement of the implant, however, another source of bacteria is the blood. (biomedcentral.com)
- The vascular loop structure within growing long bones slows the blood flow through the region, localizing bacteria that may be in the blood. (biomedcentral.com)
Morphology1
- however, the rough morphotype changed to a nonfibrillar type with a smooth colony morphology after repeated subculturing in broth. (microbiologyresearch.org)
Nutrient agar1
- glucose, maltose and lactose, an oxidase test was performed and the strains were streaked onto nutrient agar plates which were incubated at 22 C. The strains, which were oxidase positive, fermented only glucose and maltose, failed to grow on nutrient agar plate at 22 C, were reported as Neisseria meningitidis. (jpgmonline.com)
Mucoid colonies1
- Some encapsulated Klebsiella species form glistening mucoid colonies of viscid consistency. (scirp.org)
Cultures9
- His perioperative blood cultures remained negative. (cdc.gov)
- Samples for blood and synovial fluid cultures were obtained and anti-microbial therapy with intravenous cefuroxime was started. (cdc.gov)
- Overnight cultures from BAP were plated on Mueller-Hinton (MH) agar, and a 1-μg oxacillin (OX) disk was placed on the inoculated plate. (cdc.gov)
- Live exam (gill and skin biopsies) with blood cultures were taken from sixteen fish - four goldfish or orandas ( Carassius auratus ), three gulf sturgeon ( Acipenser oxyrichus de sotoi ), and nine koi ( Cyprinus carpio ). (vin.com)
- Actinobaculum schaalii was isolated in 12 urine cultures, 21 blood cultures, and 7 deep tissue biopsies. (biomedcentral.com)
- Both tests and agar plated cultures were performed on catheterized urine samples. (biomedcentral.com)
- Blood, urine, and sputum samples were collected for aerobic and anaerobic cultures. (rcjournal.com)
- Necrotizing pneumonia was diagnosed, and the patient was started on broad-spectrum antibiotics (aztreonam and clindamycin) to cover all Gram-positive and Gram-negative organisms, pending the outcome of the sputum and blood cultures. (rcjournal.com)
- Three anaerobic blood cultures all grew GCBHS. (rcjournal.com)
Colorless1
- Sorbitol-negative colonies will appear colorless on SMAC. (cdc.gov)
Rods2
- The bacterial colonies are composed of small Gram negative rods. (avma.org)
- Observation of tiny colonies of Gram-positive, catalase-negative coccoid rods triggered molecular identification based on 16S rRNA gene sequencing. (biomedcentral.com)
Sterile1
Sputum2
- Overall, sputum specimens are observed for mucopurulent strands, leukocytes, and blood and culture results. (medscape.com)
- Culture of the sputum on blood agar frequently reveals characteristic colonies, and identification is made by various serologic or biochemical tests. (medscape.com)
Selective2
- Nasal swabs were first examined for proper labeling and integrity, and then plated on mannitol salt agar (MSA), a selective media for the isolation of S. aureus . (cdc.gov)
- Mannitol salt agar (MSA) is a selective, differential, and indicator medium used to isolate and identify Staphylococcus aureus from the clinical specimen. (3ia-technology.com)
Complete lysis1
- That's because Strep agalactiae makes a toxin called beta-hemolysin, that causes complete lysis of the hemoglobin in the red blood cells, making them blood agar change color from red to transparent yellow around the colonies. (osmosis.org)
Staphylococcus5
- With the CAMP test, Strep agalactiae is grown with Staphylococcus aureus on the same blood agar. (osmosis.org)
- Stored clinical isolates of Acinetobacter baumannii (n =4), Candida albicans (n = 5), Haemophilus influenzae (n =5), Klebsiella pneumoniae (n =4), methicillin-resistant Staphylococcus aureus (n=3), Pseudomonas aeruginosa (n = 2), and Streptococcus pneumoniae (n = 7) were subcultured on horse blood agar, incubated at 35 degrees C overnight, then inoculated into trypticase soy broth to a density of 1 McFarland standard. (nih.gov)
- Using a new swab, select one colony from a pure culture of Staphylococcus aureus. (3ia-technology.com)
- Staphylococcus aureus , ranging from10 4 to 10 9 colony forming units, was injected into a tail vessel. (biomedcentral.com)
- A hematogenous infection, often from Staphylococcus aureus, originates from a secondary infection, most often from the skin, gums/teeth, or urinary tract, [ 1 ] and has traveled through the blood to the bone and implant. (biomedcentral.com)
Diameter2
- Individual colonies on agar are round, convex, and 1-4 mm in diameter with a sharp border. (3ia-technology.com)
- Round, irregular, whitish and medium sized colonies (2-6 mm i diameter). (vetbact.org)
Corynebacterium1
- McLeod's agar plate culture of Corynebacterium diphtheriae subsp. (cdc.gov)
Mannitol1
- Mannitol salt agar is best prepared from ready-to-use dehydrated powder, available from most suppliers of culture media. (3ia-technology.com)
Broth3
- One to two drops of whole blood was sterilely injected into 1 mL Brain Heart Infusion (BHI) broth. (vin.com)
- Dilutions of unfractionated heparin (containing 250- 7500 U) and 100 microL of the 1.0 McFarland standard broth were incubated at 35 degrees C overnight in microtitre plates and then subcultured on horse blood agar using 1 microL standard loops. (nih.gov)
- Samples were sonicated, and the supernatant was disseminated on brain heart infusion broth or blood agar. (allenpress.com)
Biochemical1
- Bacterial species were identified based on Gram stain, colony characteristics and biochemical techniques. (alliedacademies.org)
Supernatant1
- On the blood agar plates, the supernatant grew a few colonies, while the pellet grew intensive colonies. (molvis.org)
Plate6
- C. ulcerans colonies on a blood agar plate. (wikidoc.org)
- Image: Clostridium difficile colonies on a blood agar plate. (pharmaceutical-technology.com)
- Colonies may be tested with antisera directly from the plate, or subcultured to another nonselective medium (blood agar, for example) and tested the next day. (cdc.gov)
- If colonies are tested directly from the plate, O157- positive colonies should also be transferred to another medium for subsequent testing. (cdc.gov)
- Use several drops of the suspension to inoculate a #44 agar slant and/or plate. (atcc.org)
- 5 mm of the colony on blood agar plate. (tmu.edu.tw)
Organism2
- The organism can grow on blood agar. (wikipedia.org)
- If Legionella pneumonia is suspected, the organism can be cultured on charcoal-yeast agar, which contains the high concentrations of iron and sulfur required for growth. (medscape.com)
Catalase1
- Since 1999, Actinobaculum schaalii is routinely investigated at our hospital, whenever its presence is suspected due to the detection of minute grey colonies on blood agar plates and negative reactions for catalase. (biomedcentral.com)
Fungal2
- Clear adhesive tape touches a mold colony, picking up fungal hyphae and pressed into one drop of LCB on a microscope slide. (slideshare.net)
- Under a magnification of 30X, this image depicted a close view of two Candida albicans fungal colonies, which had been cultivated on beef extract blood agar (BEBA). (cdc.gov)
Laboratory3
- Blood was not sent for culture to the microbiology laboratory. (jpgmonline.com)
- Laboratory agar plated culture is the gold standard to establish SBU. (biomedcentral.com)
- Thus, these materials come in contact with f luids such as saliva and / or blood, and therefore can be contaminated between the office and laboratory prosthesis. (bvsalud.org)
Inhibitory1
- 2] As disc method is generally not regarded satisfactory, minimum inhibitory concentration (MIC) of sulphadiazine was determined using Abbot's agar dilution technique. (jpgmonline.com)
Counts2
- Colony counts yielding single type of bacterial growth of 105 CFU/ml were deemed significant. (banglajol.info)
- The initial white blood cell counts ranged from 500/μL to 37,000/μL (mean + SD, 12,360 + 12,200/μL) and 6 patients had leukopenia. (tmu.edu.tw)
Urine1
- It has also been recovered from other human clinical specimens than urine such as blood, but its pathogenic potential remains unknown. (biomedcentral.com)
Plates4
- It is a facultative anaerobe, grows best in 5 to 10% carbon dioxide, and forms pinpoint colonies on blood agar plates. (nih.gov)
- These plates were reexamined the next day, and any yellow or gold colonies were subcultured to BAP. (cdc.gov)
- The deposits were streaked onto blood agar, chocolate agar and Mac-Conkey agar plates and smears were prepared, which were stained by Gram's stain. (jpgmonline.com)
- The blood agar and chocolate agar plates were kept in a candle jar and all plates were incubated at 37 C for 24 hours. (jpgmonline.com)
Growth2
- Bacterial identification and antibiotic sensitivities would then be determined from colony growth on blood agar. (vin.com)
- After 24 hours in the bottle warmer, the dip slide was evaluated for colony forming units by a trained research nurse, and the result was reported as 'no growth' or 'growth' (visible colonies). (biomedcentral.com)
Reveals1
- Blood workup reveals leukocytosis and elevated C-reactive protein. (osmosis.org)
Strains1
Characteristics1
- Colony characteristics and Grams technique were used to differentiate the organisms. (bvsalud.org)
Chocolate1
- Chocolate agar - heated blood agar due to red cell lysis. (abcmedicalnotes.com)
Pathogens2
- The data indicated that the potential pathogens identified in the blood samples were associated with translocation from the gut, suggesting the presence of bacteremia, which transcriptome data suggested may induce or aggravate the acute-phase response observed during EVD. (asm.org)
- IMPORTANCE Our results highlight the identification of an array of pathogens in the blood of patients with Ebola virus disease (EVD). (asm.org)
Medium1
- When cultivated on a medium called blood agar, Strep agalactiae colonies cause beta hemolysis , also called complete hemolysis . (osmosis.org)
Primary1
Peripheral blood2
- A novel oligosaccharide compound AVR-25 selectively binds to the TLR4 protein (IC 50 = 0.15 µM) in human peripheral blood monocytes and stimulates IL-10 production. (nature.com)
- Throat swabs were taken from deceased EVD patients, and peripheral blood samples were analyzed that had been taken from patients when they presented at the treatment center with acute illness. (asm.org)
Protein1
- A decrease in serum C-reactive protein (CRP) and bacterial colony forming unit (CFU) confirmed improved bacterial clearance. (nature.com)
Coli1
- Test sorbitol- negative colonies selected from SMAC with E. coli O157 antiserum or latex reagents (O157 antibody-coated latex and control latex) according to the procedures recommended by the manufacturer (5). (cdc.gov)
Horse1
- Blood agar - 10% horse blood. (abcmedicalnotes.com)