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  • involve biofilm
  • This bacterium is commonly isolated from infections thought to involve biofilm formation, including those associated with burn wounds, keratitis, urinary catheters, otitis media, and pneumonia in patients with cystic fibrosis ( 6 ). (asm.org)
  • aureus
  • It was previously shown that the P. aeruginosa exoproduct 4-hydroxy-2-heptylquinoline-N-oxide (HQNO) suppresses the growth of S. aureus and provokes the emergence of small-colony variants (SCVs). (inrs.ca)
  • Analysis of gene expression revealed that exposure of a prototypical S. aureus strain to HQNO activates SigB, which was leading to an increase in the expression of the fibronectin-binding protein A and the biofilm-associated sarA genes. (inrs.ca)
  • Experiments using culture supernatants from P. aeruginosa PAO1 and a double chamber co-culture model confirmed that P. aeruginosa stimulates biofilm formation and activates SigB in a S. aureus strain isolated from a CF patient. (inrs.ca)
  • Furthermore, the supernatant from P. aeruginosa mutants unable to produce HQNO induced the production of biofilms by S. aureus to a lesser extent than the wild-type strain only in a S. aureus SigB-functional background. (inrs.ca)
  • FIG. 2 Patterns of DNA synthesis in S. epidermidis drip-flow (A) and capillary (B) biofilms and overlay of DNA synthetic activity and protein synthetic activity in the same S. aureus colony biofilm (C and D)‏. In panels A and B, green areas are due to BrdU labeling and red areas are due to rhodamine B counterstain. (igem.org)
  • The term biofilm, originally used in technical and environmental microbiology, was introduced into medicine in 1985 by Costerton, following observations that Staphylococcus aureus ( S. aureus ) formed biofilms on cardiac pacemaker leads [ 3 , 4 , 5 ]. (pubmedcentralcanada.ca)
  • organisms
  • There is evidence that some of these chemical signals, produced by cells and passed through their outer membranes, may be interpreted not just by members of the same cell species, but by other microbial species that are part of the same biofilm community - and perhaps even by more complex organisms in some cases. (montana.edu)
  • Biofilms are generally a group of living organisms that have a protective matrix of metallo-mineral and organic molecule coating. (ndnr.com)
  • This complex protects the organisms from anti-infective therapies, allowing them to create a "super-biotic" organism colony. (ndnr.com)
  • Recent physiological evidence supports this idea, showing a change in quorum-sensing signal profiles comparing free-swimming and biofilm P. aeruginosa organisms ( 46 ). (asm.org)
  • What we learn with respect to the mechanisms of biofilm formation in V. cholerae could be applicable to other organisms because biofilm formation is so prevalent and it is so important for clinical, environmental, and industrial processes," she said. (ucsc.edu)
  • The composition of the ECM varies depending on the organisms within the biofilm, but in general it includes microbial-derived complex polysaccharides, proteins, lipids and nucleic acids [ 1 ]. (pubmedcentralcanada.ca)
  • anti-biofilm
  • The magnitude of the biofilm problem and its impact on medical and financial aspects of modern hospital medicine have triggered the investment of major efforts to develop novel anti-biofilm strategies based on thorough, in depth analyses of the microbial transformation cascades. (mdpi.com)
  • aeruginosa biofilms
  • These results suggest that redox imbalance is a major factor driving the morphogenesis of P. aeruginosa biofilms and that wrinkling itself is an adaptation that maximizes oxygen accessibility and thereby supports metabolic homeostasis. (caltech.edu)
  • Oxygen gradients and local anaerobic conditions have also been measured in P. aeruginosa biofilms grown in vitro ( 20 , 24 ). (asm.org)
  • None of those studies, however, focused on the role of oxygen limitation in particular in the protection of P. aeruginosa biofilms. (asm.org)
  • formation
  • Robust colony formation by Bacillus subtilis is recognized as one of the sessile, multicellular lifestyles of this bacterium. (mdpi.com)
  • In this study, we generated in-frame deletions of the five genes located in the vps intergenic region, termed rbmB to - F ( r ugosity and b iofilm structure m odulators B to F) in the rugose genetic background, and characterized the mutants for rugose colony development and biofilm formation. (asm.org)
  • It has been proposed that this facultative human pathogen uses biofilm formation and phenotypic variation as survival strategies ( 63 , 64 , 71 ). (asm.org)
  • Here we review the different approaches that have been developed to control biofilm formation and removal, focusing on the utilization of acoustic energy to achieve these objectives. (mdpi.com)
  • Although biofilm formation and the conversion to mucoidy are both important aspects of CF pathogenesis, the relationship between them is at the present unclear. (asm.org)
  • Pelzer ES, Allan JA, Theodoropoulos C, Ross T, Beagley KW, Knox CL (2012) Hormone-Dependent Bacterial Growth, Persistence and Biofilm Formation - A Pilot Study Investigating Human Follicular Fluid Collected during IVF Cycles. (plos.org)
  • This was confirmed by heterologous expression of the respective QQ proteins in Klebsiella oxytoca M5a1 and monitoring biofilm formation in a continuous flow cell system. (frontiersin.org)
  • Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. (springer.com)
  • OscR increases bacterial motility and decreases the formation of sticky cell groupings called biofilm, Yildiz said. (ucsc.edu)
  • Yildiz has been investigating the network of genetic interactions that regulates biofilm formation. (ucsc.edu)
  • OscR blocks the toppling dominos that lead to biofilm formation. (ucsc.edu)
  • biomass
  • This matrix, referred to as the slime layer, glycocalyx, or extracellular polymeric substance (EPS) matrix, can comprise up to 90% of the biofilm biomass ( 8 ). (asm.org)
  • abiotic
  • In this study, we report that the overproduction of alginate affects biofilm development on an abiotic surface. (asm.org)
  • Escherichia
  • A major component of the Escherichia coli biofilm matrix is PGA, a linear polymer of N -acetyl- d -glucosamine residues in β(1,6) linkage. (asm.org)
  • genes
  • Numerous pathways and genes are responsible for the architecturally complex colony structure development. (mdpi.com)
  • Taken together, these results indicate that vps intergenic region genes encode proteins that are involved in biofilm matrix production and maintenance of biofilm structure and stability. (asm.org)
  • We then performed transcriptome analysis of ATCC 27853 and PAO1 using RNA-seq and compared the expression of orthologous genes to understand the functional genome and the genomic details underlying the distinctive colony morphogenesis. (biomedcentral.com)
  • Genetic determinants responsible for biofilm-associated antibiotic resistance include components of the quorum-sensing system ( fsrA , fsrC , and gelE ) and two glycosyltransferase (GTF) genes ( epaI and epaOX ). (asm.org)
  • however, genes involved in biofilm-associated antibiotic resistance have not been reported. (asm.org)
  • Podholova K, Plocek V, Resetarova S, Kucerova H, Hlavacek O, Vachova L, Palkova Z (2016) Divergent branches of mitochondrial signaling regulate specific genes and the viability of specialized cell types of differentiated yeast colonies . (biocev.eu)
  • development
  • Compared to surface attachment and mature biofilm development, the molecular mechanisms of the detachment process are largely unknown. (asm.org)
  • Advances in biofilm biology have been made over decades for model bacterial species, and include characterizations of social behaviors and cellular differentiation during biofilm development. (biomedcentral.com)
  • Here, we investigated the development, composition and dynamics of biofilms formed by the haloarchaeon Haloferax volcanii DS2. (biomedcentral.com)
  • Furthermore, although exopolysaccharide production is required for the development of a three-dimensional biofilm, inappropriate exopolysaccharide production leads to inefficient colonization of the infant mouse intestinal epithelium by flagellar mutants. (mendeley.com)
  • Perturbations in the environmental provision, endogenous production, and utilization of electron acceptors affect colony development in a manner consistent with redox control. (caltech.edu)
  • Consistent with convention, I will use the term "Phase-1" biofilms for those in earlier stages of development that are less significantly pathogenic (at times even considered normal), and I will use the term "Phase-2" biofilms for those that are in later and more complex stages of development and which are universally pathogenic and impediments to cure. (ndnr.com)
  • Such colonies display complex spatial and temporal development patterns that are the result of both cell-cell interactions and cell-environment interactions. (google.com)
  • There is increasing evidence suggesting that biofilm populations also activate distinct mechanisms of resistance during biofilm development. (asm.org)
  • Our research group studies development and differentiation of yeast colonies and biofilms. (biocev.eu)
  • bacterium
  • For example, in the late 1990s an investigation of a biofilm community the marine bacterium Pseudoalteromonas revealed two physiologically distinct subpopulations. (montana.edu)
  • 2016
  • 2016. Adhesion and removal kinetics of Bacillus cereus biofilms on Ni-PTFE modified stainless steel. (umass.edu)
  • 2016 ). The biofilm matrix is composed of multiple species and a mixture of components such as EPS, protein, nucleic acids, and other substances. (springer.com)
  • volcanii
  • The developmental processes, functional properties and dynamics of H. volcanii biofilms provide insight on how haloarchaeal species might persist, interact and exchange DNA in natural communities. (biomedcentral.com)
  • Because H. volcanii has one of the most advanced genetic systems for any archaeon, the phenotypes reported here may promote the study of genetic and developmental processes in archaeal biofilms. (biomedcentral.com)
  • species
  • Another experiment, Scheu explains, is to compare different species of desmids by adding tiny fluorescent beads to their environment and using a fluorescence microscope to see if any desmid biofilm gathers up the beads as it grows. (skidmore.edu)
  • These cryptic species have, until now, largely been grouped together in ecological studies, and so little is known about their individual colony densities, foraging ranges or habitat requirements, which can be influenced by land use at a landscape scale. (jove.com)
  • We then used microsatellite genotyping to identify sisters and estimate colony densities, and related both proportions of cryptic species and their colony densities to the composition of the landscape surrounding the fields. (jove.com)
  • We also found that the cryptic species responded differently to surrounding landscape composition: both relative proportions of B. cryptarum in samples and colony densities of B. lucorum were negatively associated with the amount of arable land in the landscape, while proportions and colony densities of other species did not respond to landscape variables at the scale measured. (jove.com)
  • This suggests that the cryptic species have different ecological requirements (which may be scale-dependent) and that oilseed rape can be an important forage resource for many colonies of bumblebees. (jove.com)
  • pathogens
  • Biofilms which consist of opportunistic pathogens have been found to exist in cold and hot water and on shower heads, water taps, and even water filters. (emaxhealth.com)
  • grown
  • The biofilm was grown in the same capillary reactor system described above. (hypertextbookshop.com)
  • The first was positioned at the base of the biofilm just a little above the stainless steel slide on which it was grown. (hypertextbookshop.com)
  • In Maryland Sea Grant's interactive lesson Biofilms and Biodiversity, you will evaluate biofilm communities grown on plexiglass discs suspended vertically in the Baltimore Inner Harbor water. (pearltrees.com)
  • surface
  • Depending on the environmental conditions, these secreted components of the colony biofilm can also promote the flagellum-independent surface spreading of B. subtilis , called sliding. (mdpi.com)
  • Now when a hydrogen peroxide solution is introduced into this flow cell, the concentration of hydrogen peroxide that is being delivered to the surface of the biofilm and the concentration of hydrogen peroxide that reaches the base of the biofilm can be simultaneously recorded in time. (hypertextbookshop.com)
  • Here, we demonstrate how exposing the surface of mature biofilm colonies to concentrated ethanol, saline or glucose solutions results in topographical changes that enable their wettability. (readbyqxmd.com)
  • Biofilms are bacterial colonies that are attached to a surface. (google.com)