TNF-α-mediated bystander assays used a murine coculture system of primary colonic epithelial cells and E. faecalis -infected macrophages ( in vitro ), with an interleukin 10 (IL-10)-deficient mouse model of colon cancer that involves long-term colonization with E. faecalis ( in vivo ). (aacrjournals.org)
dimension of cell proliferation To research the consequences of alkylating agent-induced DNA harm on cell routine in colonic epithelial cells, proliferative activity was assessed using the Click-iT EdU Alexa Fluor 555 Imaging package (Life Systems) according to the manufacturers guidelines. (bios-mep.info)
Colonic epithelial cells proliferation of group IV (DPE 250 mg/kgBW) were significantly lower than III and V groups. (biomedcentral.com)
These findings indicate that the DPE could inhibit colonic epithelial cells proliferation through p53 pathway independently. (biomedcentral.com)
Dextran sulfate sodium2
The inhibitory effects of the doenjang prepared with the grain-type meju using mixed starter cultures were investigated in azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colon carcinogenesis mice model. (carcinogenesis.com)
METHODS: To investigate the effect of astragaloside IV against CAC and the underlying mechanism, C57 mice were treated with (20, 40, 80 mg/kg) astragaloside IV while CAC was induced by intraperitoneal injection of 10 mg/kg azoxymethane (AOM) and ad libitum consumption of 2% dextran sulfate sodium salt (DSS). (bvsalud.org)
Aberrant crypts2
0.001) of aberrant crypts, preneoplastic colonic lesions, compared to animals only receiving AOM injection. (uwaterloo.ca)
We also examined whether lactic acid bacteria increased the production of the cancer-specific anti-tumor cytokine, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), in human, and examined whether the RB-reactivating drinks with lactic acid bacteria decreased azoxymethane-induced rat colonaberrant crypt foci (ACF) and aberrant crypts (ACs) in vivo. (bvsalud.org)
Colon8
Silibinin dose-dependently decreased AOM-induced colonic cell proliferation, evidenced by proliferative cell nuclear antigen and cyclin D1 immunohistochemical staining, and induced apoptosis in these colon tissues, evidenced by terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling staining and cleaved poly(ADP-ribose) polymerase. (nih.gov)
Treatment of this mouse model with the procarcinogen azoxymethane (AOM) leads to formation of dysplastic microadenomas in the proximal but not in the distal colon. (wikipedia.org)
This study was conducted to investigate the chemopreventive potential of camel ( Camelus dromedarius ) and bovine milk as well as the impact of fermenting these milks with Lactobacillus acidophilus and Streptococcus thermophilus against early colon carcinogenesis as measured by the reduction of aberrant crypt foci (ACF) in azoxymethane (AOM)-treated Fischer 344 rats. (scialert.net)
Azoxymethane (AOM) is an organotrophic genotoxic colon carcinogen that is activated by cytochrome P450 and then hydroxylated. (scialert.net)
Body weight, colon length, and colon weight of mice were determined, and colonic tissues were histologically evaluated. (carcinogenesis.com)
Administration of the doenjang using probiotic mixed starter cultures ameliorated the symptoms of colon cancer, and reduced the incidence of neoplasia, and reduced the levels of serum proinflammatory cytokines such as interleukin-6, and tumor necrosis factor-α and inducible nitric oxide synthase and cycloooxygenase-2 expression levels in colonic tissue. (carcinogenesis.com)
These findings indicate that the doenjang attenuated colon carcinogenesis induced by AOM and DSS by ameliorating the symptoms of colon cancer, reducing the occurrence of neoplasia, regulating proinflammatory cytokine levels, and controlling the expressions of inflammation- and apoptosis-related genes in the colonic tissue. (carcinogenesis.com)
Mouse colitis associated colon cancer (CAC) was induced firstly by azoxymethane (AOM) and followed by administration of drinking water containing 5 % dextran sodium sulfate (DSS) in a cycle protocol, each cycle consisted of seven days of 5 % DSS in the drinking water and followed by seven days of regular water. (biomedcentral.com)
Inhibition1
1980. Inhibition by bran of the colonic cocarcinogenicity of bile salts in rats given dimethylhydrazine. (cdc.gov)
Mucosal2
These findings indicate that the colonic mucosal injury induced by FC-exposure are associated with oxidative stress generation, inflammation response and cell apoptosis, as well as the changes in gut microbes diversity and composition. (bvsalud.org)
RESULTS: Our results showed that astragaloside IV significantly improved AOM/DSS-induced CAC mice by inhibiting colonic shortening, preventing intestinal mucosal damage, reducing the number of tumors and, the expression of Ki67 protein. (bvsalud.org)
Mice3
In the experiment involving carcinogenesis, mice were given two subcutaneous injections of azoxymethane (AOM), followed by a two week incubation period, and subsequently exposed to bLf or the control diet. (uwaterloo.ca)
In the present study, we developed a novel mouse model of malignant lesions in the biliary ducts induced by the administration of the carcinogen azoxymethane to obese C57BLKS/J-db/db mice. (bvsalud.org)
Azuine, M. A. and Bhide, S. V. Chemopreventive impact of turmeric in opposition to abdomen and pores and skin tumors induced by chemical carcinogens in Swiss mice. (fairpeel.com)
Rodent2
1988. Species-specific response to the rodent carcinogens1,2-dimethylhydrazine and 1,2-dibromo-3-chloropropane in rodent bone-marrow micronucleus assays. (cdc.gov)
Various animal models, including carcinogen-induced and genetically engineered rodent models, have been established to clarify the mechanisms underlying cholangiocarcinoma development. (bvsalud.org)
Metabolism1
Further metabolism by colonic bacteria that produce β-glucuronidase generates methyldiazonium, which can initiate cancer by inducing mutagenic lesions in target cells by the alkylation of DNA at the O6 position of guanine 17 . (scialert.net)
Rats1
Based on our recent silibinin efficacy studies in human colorectal cancer cells, we investigated the effects of its dietary feeding on azoxymethane (AOM)-induced aberrant crypt foci (ACF) formation and associated biomarkers in male Fisher 344 rats. (nih.gov)
Expression3
The serum levels of proinflammatory cytokines as well as the expression of inflammation- and apoptosis-related genes in colonic tissue were also analyzed. (carcinogenesis.com)
In addition, it increased Bax and reduced Bcl-2 expression levels and increased p21 and p53 expression in the colonic tissues. (carcinogenesis.com)
Additionally, the serum lipopolysaccharide (LPS) contents increased in FC-exposed groups when compared with the control group, while the expression of colonic tight junction proteins (TJPs), such as Claudin-1 and Occludin were decreased. (bvsalud.org)
Results1
These results highlight the systems where colonic Lgr5+ stem cells react to cancer-causing environmental elements. (bios-mep.info)
Carcinogenesis1
12. Effect of different levels of omega-3 and omega-6 fatty acids on azoxymethane-induced colon carcinogenesis in F344 rats. (nih.gov)
Tumorigenesis1
Thus, several critical genes for tumorigenesis are potential targets for carcinogens and radiation that can induce point mutations at low doses. (nih.gov)
Aberrant1
The effects of dietary selenomethionine on polyamines and azoxymethane-induced aberrant crypts. (nih.gov)
Supplementation3
We evaluated the effects of dietary selenomethionine supplementation on colonic polyamine levels and the ability of L-selenomethionine supplementation to modulate the carcinogenic activity of azoxymethane (AOM) in the rat colon. (nih.gov)
4. Rapid incorporation of ω-3 fatty acids into colonic tissue after oral supplementation in patients with colorectal cancer: a randomized, placebo-controlled intervention trial. (nih.gov)
However, evaluation of the colonic tissue polyamine levels between control and treated groups showed no significant difference. (nih.gov)
Colon cancer2
3, 11- 14 This Ob-Rb expression is preserved in human colonic adenomas and carcinomas as well as in human colon cancer cell lines. (bmj.com)
In this study, the authors identified the mechanism by which P. anaerobius promotes cancer using the potent carcinogen azoxymethane (AOM) mouse model of colon cancer. (elsevierpure.com)
Proliferation1
However, we have shown that although leptin stimulates epithelial cell proliferation it reduces the development of carcinogen induced preneoplastic lesions in the rat colon. (bmj.com)
Targets1
The ras proto-oncogenes are targets for many genotoxic carcinogens. (nih.gov)
Mouse model1
Treatment of this mouse model with the procarcinogen azoxymethane (AOM) leads to formation of dysplastic microadenomas in the proximal but not in the distal colon. (wikipedia.org)
Levels2
However, in the azoxymethane-treated rat, where beta-catenin is frequently rendered GSK-3beta-insensitive, nabumetone failed to alter beta-catenin levels but did decrease beta-catenin nuclear localization and transcriptional activity as gauged by cyclin D1. (nih.gov)
Researchers in Hong Kong and Singapore have found that grape seed and clove bud extracts seem to cut the formation of the carcinogen acrylamide in food products made from potatoes by 60 percent. (chinabusiness360.com)