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LamininReceptors, LamininCollagen Type IVCollagenBasement MembraneCollagen Type IExtracellular MatrixFibronectinsMembrane GlycoproteinsExtracellular Matrix ProteinsCell AdhesionReceptors, CollagenIntegrinsFibrillar CollagensHeparan Sulfate ProteoglycansCollagen Type IIICells, CulturedMolecular Sequence DataIntegrin alpha1beta1Amino Acid SequenceProteoglycansMicroscopy, ElectronCollagen Type IIFluorescent Antibody TechniqueIntegrin alpha6beta1Antigens, CD29Peptide FragmentsImmunohistochemistryIntegrin alpha1Protein BindingDystroglycansCollagen Type VICell MovementCollagen Type VRNA, MessengerBinding SitesMesangial CellsFibroblastsBase SequenceHeparitin SulfateCell Adhesion MoleculesHydroxyprolineElectrophoresis, Polyacrylamide GelIntegrin alpha6Membrane ProteinsEpitheliumGlomerular Basement MembraneIntegrin alpha3beta1SkinKidney GlomerulusIntegrin alpha2ProcollagenCollagen DiseasesRecombinant ProteinsCollagen Type XVIIILutheran Blood-Group SystemAnti-Glomerular Basement Membrane DiseaseCell LineNephritis, HereditaryMicrobial CollagenaseImmunochemistryAntibodies, MonoclonalCattleCell DifferentiationCollagen Type XIFibrosisIn Situ HybridizationTumor Cells, CulturedTransforming Growth Factor beta1AntibodiesGlycoproteinsCollagenasesTransfectionTransforming Growth Factor betaBlotting, WesternMatrix Metalloproteinase 2Integrin alpha6beta4Mice, Inbred C57BLEpithelial CellsGene ExpressionGelsProtein ConformationDNAMicroscopy, Electron, ScanningHeparinGlomerular MesangiumKidneyChick EmbryoCell DivisionPepsin AVitronectinLaryngeal EdemaWound HealingGingival HypertrophyProcollagen-Proline DioxygenaseHydroxylysineOligopeptidesCartilageCloning, MolecularAutoantigens
ProteoglycansEntactinFibronectinProteinsProteinCOL4A1FibrillarExtracellular matrixMutationsIsoformsProliferationPiersonGelatinPrecursorGenesChainsSynthesisCDNATissueProgressionPhysiologicalGlobularSignificantlyCellsCrucialComponentFibroblastsHumanMouseExpressionAdditionStudyDistinctHydrophobicRoleLarge
Proteoglycans6
  • BMs are specialized structures that underlie epithelia and surround organs and mainly comprise collagen IV, laminins, and heparan sulfate proteoglycans (HSPG). (nature.com)
  • This fibrillar network is composed of many structural proteins, such as collagens, and adhesion proteins, including fibronectin and proteoglycans (perlecan, decorin) [ 13 , 14 ]. (springer.com)
  • Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. (cusabio.com)
  • Laminin type IV collagen nidogen and sulfated proteoglycans comprise the GBM [37] and laminins are heterotrimeric glycoproteins including one α one β and one γ string. (buyresearchchemicalss.net)
  • Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors. (cgenomix.com)
  • This growth factor also stimulates production of collagens, proteoglycans, elastin, fibronectin, tenascin and thrombospondin, diminishes production of extracellularly active neutral endoproteinases belonging to the matrix metalloproteinase (MMP) and serine proteinase families, and stimulates production of endogenous MMP inhibitors (tissue inhibitor of metallo-proteinase [TIMP]) and serpins (plasminogen activator inhibitor-1). (biomedcentral.com)
Entactin1
  • neuroblastoma organoids (tumour fragments of ~30-60 cells) were obtained from patient-derived xenografts (models of cancer where patient-derived neuroblastoma cells are implanted into and a mouse and allowed to develop into a tumour) and embedded in 3D Matrigel (a gelatinous protein mixture containing laminin, collagen, nidogen, heparan sulphate proteoglycan, and entactin) or collagen. (nationalchildrensresearchcentre.ie)
Fibronectin3
  • IMs are found in the space between the tissue stroma cells and contain, among other components, fibrillar collagens and fibronectin. (nature.com)
  • Extracellular matrix is a fibrillar network composed of structural (collagens) and adhesion (fibronectin) proteins crucial for tissue architecture and biological pathway Histol Histopathol 27:1515-1528, 2012 . (springer.com)
  • ECM components (i.e., fibronectin or collagens) directly interact with membrane receptors (i.e., integrins), particularly, by influencing cell behavior. (springer.com)
Proteins8
  • Type IV collagen is the major structural component of basement membranes, which contains two or three COL4A1 proteins. (wikipedia.org)
  • Biochemically, the BM consists of four big ECM proteins: collagen type IV, laminin, nidogen, and perlecan (Figure S7). (idhinhibitor.com)
  • Isolated human islets were cultured under a hypoxic atmosphere (2% oxygen) in media supplemented with either solubilized porcine pancreatic ECM (ppECM) or a mixture of human ECM proteins composed of collagen-IV, laminin-521, and nidogen-1 (hEPM). (ox.ac.uk)
  • Hence, the commentary highlights the significance of dermal-epidermal junction proteins, specifically collagen IV, a target of a newly developed complex DL-linefade. (heraldopenaccess.us)
  • These zones are mainly composed of collagen IV, plectin, laminin (proteins of BM), and nidogen in the upper regions, collagen IV, VI, anchoring fibrils, and heparan sulfate proteoglycan (proteins of LD) [8]. (heraldopenaccess.us)
  • Although these products help treat skin issues, niacinamide usually targets the collagen I and III proteins of the inner dermal layer of the skin that causes skin aging [9]. (heraldopenaccess.us)
  • Contrary to reef building corals, the red coral organic matrix possesses a large number of collagen-like proteins. (biomedcentral.com)
  • [2] Nutritionally, collagen and gelatin are poor quality protein since they do not contain all the essential amino acids that the human body requires - they are not complete proteins . (wikidoc.org)
Protein11
  • Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. (unifi.it)
  • Collagen alpha-1(IV) chain (COL4A1) is a protein that in humans is encoded by the COL4A1 gene on chromosome 13. (wikipedia.org)
  • Accordingly, it was demonstrated that protein expression of collagen type IV in human vascular smooth muscle cells is regulated by both SMAD3 protein and TGFβ mediated stimulation of mRNA. (wikipedia.org)
  • Lastly, we revealed that addition of type IV collagen protein induced myofibroblast proliferation and migration in monolayer culture and increased the formation of mesenchymal-epithelial septal-like structures in co-culture. (biomedcentral.com)
  • d - i Type IV collagen protein and mRNA expression in the lung. (biomedcentral.com)
  • The major structural protein of basement membrane, collagen type IV, in the lens capsule was substantially altered in the absence of SPARC. (silverchair.com)
  • Using our founded cell and knockout/transgenic mouse versions resembling human being NS harboring the R246Q or C321R mutation respectively we've demonstrated that both R246Q and C321R mutations trigger faulty secretion of laminin-521 from podocytes towards the GBM [49 50 Furthermore we've demonstrated how the misfolded C321R mutant protein induces podocyte ER tension and proteinuria [50]. (buyresearchchemicalss.net)
  • The induction of mRNA and protein of CYP2C19 shows a large interindividual variability en t In the human liver. (c-kitinhibitors.com)
  • Collagen IV is a known significant anchoring protein found in the lamina densa region of the DEJ that provides a framework to the interconnecting network of dermis-epidermis. (heraldopenaccess.us)
  • 29,4 kDa) from soy protein induction. (kemdikbud.go.id)
  • Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals , [1] making up about 25% of the total protein content. (wikidoc.org)
COL4A14
  • COL4A1 is a subunit of the type IV collagen and plays a role in angiogenesis. (wikipedia.org)
  • COL4A1 belongs to the type IV collagen family and contains three domains: a short N-terminal domain, a long triple-helical 7S domain at its center, and a non-collagenous 1 (NC1) domain at its C-terminal. (wikipedia.org)
  • In humans, a novel mutation of the COL4A1 gene coding for collagen type IV was found to be associated with autosomal dominant congenital cataract in a Chinese family. (wikipedia.org)
  • This report of a new mutation in the COL4A1 gene is the first report of a non-syndromic autosomal dominant congenital cataract that highlights an important role for collagen type IV in the physiological and optical properties of the lens. (wikipedia.org)
Fibrillar2
Extracellular matrix2
Mutations3
  • Mutations that affect the GBM's collagen α3α4α5(IV) components cause Alport syndrome (kidney disease with variable ear and eye defects) and its variants, including thin basement membrane nephropathy. (wustl.edu)
  • Mutations in LAMB2 that impact the synthesis or function of laminin α5β2γ1 (LM-521) cause Pierson syndrome (congenital nephrotic syndrome with eye and neurological defects) and its less severe variants, including isolated congenital nephrotic syndrome. (wustl.edu)
  • The very different types of kidney diseases that result from mutations in collagen IV vs. laminin are likely due to very different pathogenic mechanisms. (wustl.edu)
Isoforms1
  • We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
Proliferation2
Pierson2
  • and laminin-β2 (trigger Pierson symptoms (OMIM 609049) which can be seen as a CNS/diffuse mesangial sclerosis serious ocular abnormalities and neurodevelopmental impairments [34-36]. (buyresearchchemicalss.net)
  • Once trimers are secreted in to the extracellular space they polymerize to create the supramolecular laminin network via relationships among the NH2-termini from the brief hands (LN domains) [40 41 null mice recapitulate Pierson symptoms [42-47]. (buyresearchchemicalss.net)
Gelatin2
  • If collagen is partially hydrolyzed , the three tropocollagen strands separate into globular, random coils , producing gelatin , which is used in many foods , including flavored gelatin desserts. (wikidoc.org)
  • [3] Collagen normally converts to gelatin, but survived due to the dry conditions. (wikidoc.org)
Precursor1
  • Weak sequence homologies (25%) were detected between some regions of nidogen, the LDL receptor, thyroglobulin and the EGF precursor. (unifi.it)
Genes1
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
Chains2
  • Compared to ECs, pancreatic pericytes produce significantly higher levels of α2 and α4 laminin chains, which constitute the peri-islet and vascular BM. (nature.com)
  • Laminin trimerization happens in the endoplasmic reticulum (ER) and requires association from the three Baricitinib (LY3009104) chains along their laminin coiled-coil domains to create the lengthy arm [39]. (buyresearchchemicalss.net)
Synthesis2
CDNA1
  • The whole amino acid sequence of nidogen was deduced from cDNA clones isolated from expression libraries and confirmed to approximately 50% by Edman degradation of peptides. (unifi.it)
Tissue2
  • Obesity is defined as an expansion of white adipose (WAT) tissue that is associated with low grade inflammation which possibly contributes to the development of health complications such as diabetes, liver pathologies, dyslipidemia, cardiovascular diseases as well as some cancers [ 2 - 4 ]. (springer.com)
  • porcine (pig) tissue is also widely used for producing collagen sheet for a variety of surgical purposes. (wikidoc.org)
Progression1
  • Hitherto the involvement of the MECs in mammary gland orchestration and morphogenesis [ 4 ] and in affording fortification against tumour progression and invasion [ 5 , 6 ] has re-established the attention in studying and characterizing the MECs. (biomedcentral.com)
Physiological1
  • Static assays such as transwell migration in Boyden chambers 4 are hindered by effects of gravity, lack of physiological flow, and difficulty in visualizing and analysing the neutrophils at each stage of transmigration. (nature.com)
Globular1
  • The data support a previously proposed dumb-bell model of nidogen by demonstrating a large N-terminal globular domain (641 residues), five EGF-like repeats constituting the rod-like domain (248 residues) and a smaller C-terminal globule (328 residues). (unifi.it)
Significantly2
Cells1
  • Human umbilical vein endothelial cells formed confluent vessels against collagen I and geltrex mix, a mix of basement membrane extract and collagen I. TNF-α-stimulation of vessels upregulated inflammatory cytokine expression and promoted neutrophil transmigration. (nature.com)
Crucial1
  • Collagens are crucial for the ECM architecture and particularly collagen I, III and VI are key components in animal tissues. (springer.com)
Component2
Fibroblasts1
  • Fibroblasts produce TIMPs (1-4), which were previously called human fibroblast collagenase inhibitors. (biomedcentral.com)
Human1
  • In addition to IL-4, `biologicals' such as humanized anti-TGF-β antibodies and recombinant human interferons are, accordingly, being tested as a treatment for fibrotic diseases. (biomedcentral.com)
Mouse1
Expression1
Addition1
  • So, all MMP's may be regarded as derivatives of a 4-domain prototype structure, formed either by addition or deletion of regulatory domains. (periobasics.com)
Study3
  • The study was based on a community cohort study (the Malmo Diet and Cancer study) and four additional randomized controlled trials of primary prevention cohorts (JUPITER and ASCOT) and secondary prevention cohorts (CARE and PROVE IT-TIMI 22). (wikipedia.org)
  • Our study showed that type IV collagen and, therefore the basement membrane, play fundamental roles in coordinating alveolar morphogenesis. (biomedcentral.com)
  • 4 In a previous study, our group has shown that there is a significant correlation between the amount of ECM underneath the inner wall of Schlemm's canal (SC) and the degree of axon loss in the ONH of glaucomatous eyes. (arvojournals.org)
Distinct1
  • A dermal-epidermal junction is an important interface of the skin with four distinct zones, Basement Membrane (BM), Hemidesmosomes (HD), Lamina Lucida (LL), and Lamina Densa (LD) [7]. (heraldopenaccess.us)
Hydrophobic1
  • 1987 ). This superfamily of proteases is defined by the presence of a Zn 2+ ion at the catalytic center, which is coordinated by three histidine residues in the zinc-binding consensus sequence HExxHxxGxxH that is present in all proteolytically active metzincins, and a characteristic, strictly conserved methionine-containing tight 1,4 beta turn forming a hydrophobic cleft for the catalytic zinc ion (Bode et al. (evelinvahter.com)
Role2
  • Inhibition of neutrophil proteases inhibited neutrophil transmigration on geltrex mix, but not collagen I. These findings highlight the important role of the ECM in determining cell phenotype and response to inhibitors. (nature.com)
  • however, the specific role of type IV collagen in regulating alveologenesis remains unknown. (biomedcentral.com)
Large1
  • Teneurins have a single transmembrane domain, a very large and cleavable extracellular domain containing eight EGF repeats, four NHL domains and more than 20 YD repeats, as well as a cleavable intracellular domain (ICD) that can be translocated to the nucleus. (biomedcentral.com)