Coli upon interactions. Medical search. Frequent questions

To make this possible in Eshcrichia coli, which is one of the best organisms to understand cellular systems comprehensively based on the vast amount of accumulation of biological knowledge, we setup an easy and reliable system for construction double knockout strains by conjugation and for analysis of their growth effects. (cam.ac.uk)
This resistance has spread to strains of E. coli and other gram-negative bacteria (Iroha et al . (scialert.net)
Most E. coli strains are harmless, but some serotypes such as EPEC, and ETEC are pathogenic and can cause serious food poisoning in their hosts, and are occasionally responsible for food contamination incidents that prompt product recalls. (wikipedia.org)
For example, some strains of E. coli benefit their hosts by producing vitamin K2 or by preventing the colonization of the intestine by pathogenic bacteria. (wikipedia.org)
E. coli and other facultative anaerobes constitute about 0.1% of gut microbiota, and fecal-oral transmission is the major route through which pathogenic strains of the bacterium cause disease. (wikipedia.org)
Furthermore, reverse transcription real-time quantitative polymerase chain reaction was used to analyze the changes of virulence genes (stx1, stx2, hly and eae) expression in representative strains of E. coli O157:H7 during mixed species biofilm formation. (spkx.net.cn)
The results showed that the motility of the three tested strains of E. coli O157:H7 was lower than that of the four strains of Pseudomonas when they were cultured separately (P (spkx.net.cn)
Instead, biofilm formation by the four Pseudomonas strains was significantly inhibited by the three strains of E. coli O157:H7 (P (spkx.net.cn)
Interactions of organic acids with Staphylococcus aureus and MRSA strains from swine mandibular lymph node tissue, commercial pork sausage meat and feces. (usda.gov)
The model was also successfully applied to E. coli K12, used for the authors' original modeling, as well as for two other E. coli strains. (iucr.org)
Assay specificity was evaluated using three E. coli K12 strains (ATCC W3110, MG1655 & DH1), 24 non-K12 E. coli and 23 bacterial genera. (who.int)

In this study, the molecular basis of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium flagella binding to epithelial cell cultures was investigated. (ed.ac.uk)
Motor mutants of E. coli O157:H7 and S. Typhimurium caused reduced haemolysis compared to wild-type, indicating that membrane disruption was in part due to flagella rotation. (ed.ac.uk)
Flagella from E. coli O157 (H7), EPEC O127 (H6) and S. Typhimurium (P1 and P2 flagella) were shown to bind to purified intracellular components of the actin cytoskeleton and directly increase in vitro actin polymerization rates. (ed.ac.uk)
We propose that flagella interactions with host cell membranes and cytoskeletal components may help prime intimate attachment and invasion for E. coli O157:H7 and S. Typhimurium, respectively. (ed.ac.uk)
The principal objective of this study was to determine the effects of Lactobacillus on the upregulation of MUC2 mucin and the subsequent inhibition of E. coli O157:H7 attachment to epithelial cells. (korea.ac.kr)
In the current study, the attachment of E. coli O157:H7 to HT-29 intestinal epithelial cells was inhibited significantly by L. acidophilus A4 and its cell extracts. (korea.ac.kr)
It is also important to note that the expression of MUC2 mucin was increased as the result of the addition of L. acidophilus A4 cell extracts (10.0 mg/ml), which also induced a significant reduction in the degree to which E. coli O157:H7 attached to epithelial cells. (korea.ac.kr)
These results indicate that MUC2 mucin and cytokines are important regulatory factors in the immune systems of the gut, and that selected lactobacilli may be able to induce the upregulation of MUC2 mucin and specific cytokines, thereby inhibiting the attachment of E. coli O157:H7. (korea.ac.kr)
The interaction between Escherichia coli O157:H7 and the common food spoilage bacterium Pseudomonas spp. (spkx.net.cn)
When co-cultured with E. coli O157:H7, the swimming motility of Pseudomonas was inhibited considerably. (spkx.net.cn)
Interaction between Escherichia coli O157:H7 and Pseudomonas during the Formation of Mixed-Species Biofilm and Its Effect on the Expression of Virulence Genes[J]. FOOD SCIENCE, 2022, 43(20): 135-147. (spkx.net.cn)
Mixed-Species Biofilm Formation of Escherichia coli O157:H7 and Pseudomonas [J]. FOOD SCIENCE, 0, (): 0-0. (spkx.net.cn)
Enterohaemorrhagic Escherichia coli O157 : H7 is a bacterial pathogen that can cause haemorrhagic colitis and haemolytic uremic syndrome. (hw.ac.uk)
On June 22, 2022, the Tennessee Department of Health (TDH) was notified of a child hospitalized with Shiga toxin-producing Escherichia coli (STEC) O157:H7 after attending a farming camp at farm A. Three days later, TDH was notified of a second hospitalized child with hemolytic uremic syndrome, whose brother had attended the same camp, prompting an investigation. (cdc.gov)
Her doctor has just told her that she has an Escherichia coli O157:H7 (commonly called E. coli ) infection, a bacterial infection that causes serious stomach and intestinal distress and is sometimes fatal, especially in children and the elderly. (cdc.gov)
In 2006, a CDC team worked with officials from California and the Food and Drug Administration (FDA) to investigate an outbreak of E. coli O157:H7. (cdc.gov)

A quantitative polymerase chain reaction assay (115 bp amplicon) specific to Escherichia coli K12 with an ABI(TM) internal control was developed based on sequence data encoding the rfb gene cluster. (who.int)

Future challenges in host-microbe interactions will be to decipher the mechanisms by which the type III secretion system required for colonization of bovine and human intestines becomes fully functional and how the bacterial effector proteins injected into the host cell and the regulation of their expression manipulate host signal transduction pathways. (cdc.gov)
Our work provides an extended understanding of the metabolic interplay in biofilms and presents a new approach of investigating complex interactions in bacterial communities on the systems level. (nature.com)
The novel DNA extraction protocol and specific E. coli K12 assay are sensitive and robust enough for detection and quantification within iron drinking water pipe biofilms, and are particularly well suited for studying enteric bacterial interactions within biofilms. (who.int)

Identification of protein-protein interactions (PPIs) is important for understanding how proteins work together in a coordinated fashion in a cell to perform cellular functions. (nature.com)
Even for Arabidopsis , known PPI data cover interactions with only about 34.55% of proteins. (nature.com)
The facility expresses and purifies proteins from E. coli , insect cells and mammalian cells. (embl.org)
Here we show that Escherichia coli bacteria expressing fibrous surface proteins, known as curli, assemble and activate factors of the human coagulation cascade at their surface. (lu.se)

E. coli is the most widely studied prokaryotic model organism, and an important species in the fields of biotechnology and microbiology, where it has served as the host organism for the majority of work with recombinant DNA. (wikipedia.org)
Commensalism is an interaction in which one organism benefits while the host remains unaffected. (proprofs.com)
In the case of E.coli in the gut, it is an example of mutualism because E.coli benefits by obtaining nutrients and a suitable environment for growth, while the host organism (the gut) also benefits from E.coli's production of certain vitamins and its role in digestion. (proprofs.com)

Antagonistic effects of lipids against the anti-Escherichia coli and anti-Salmonella activity of thymol and thymol-ß-D-glucopyranoside in porcine gut and fecal cultures in vitro. (usda.gov)
We did this for illnesses caused by Campylobacter , Escherichia coli O157, or E. coli O157, Listeria , and Salmonella bacteria. (cdc.gov)

The rate of in vivo transcription from the E. coli tRNA and rRNA promoters depends on both cellular growth rate and aminoacid availability. (dundee.ac.uk)
Escherichia coli initiator tRNA: structure-function relationships and interactions with the translational machinery. (yale.edu)

International Spread of Multidrug-Resistant Campylobacter coli in Men who have Sex with Men in Washington State and Quebec, 2015-2018. (cdc.gov)

Consensus was reached that 1) the term enterohemorrhagic E. coli based on serogroups can be misleading, 2) an alternative nomenclature based on patterns of virulence genes associated with severe disease is needed, and 3) the combination of virulence genes that make a strain pathogenic needs to be determined. (cdc.gov)
This study provided thus further insights into the interactions between EPS-LM and LTB, suggesting that EPS produced by some LAB, such as EPS produced by Ln. mesenteroides P35 strain are good candidates to inhibit E. coli toxin activity. (hal.science)

MG1655, Escherichia coli MG1655, Escherichia coli str. (string-db.org)

Bovine rectoanal junction-in vitro organ culture model system to study Shiga toxin-producing Escherichia coli adherence. (usda.gov)

The interaction of the Escherichia coli single strand binding (SSB) protein with single-stranded DNA is complex, since a number of different binding modes have been observed, with different DNA site sizes and binding properties and the transitions among these binding modes are strongly influenced by solution conditions in vitro. (utmb.edu)
Bound or free: interaction of the C-terminal domain of E. coli single-stranded DNA binding protein (SSB) with the tetrameric core of SSB. (cipps.org.au)

E. coli can live on a wide variety of substrates and uses mixed acid fermentation in anaerobic conditions, producing lactate, succinate, ethanol, acetate, and carbon dioxide. (wikipedia.org)

Butland et al: "Interaction network containing conserved and essential protein complexes in Escherichia coli. (emili-cnsb.org)

Verocytotoxin (VT)/Shiga toxin-producing Escherichia coli (VTEC/STEC) infections are a major public health concern, causing severe illnesses such as hemorrhagic colitis and hemolytic uremic syndrome (HUS). (cdc.gov)
Shiga Toxin producing Escherichia coli (STEC) are potentially highly virulent and can cause illness at levels of 10 cells if ingested by a susceptible host. (centerforproducesafety.org)
There has been an increase in the number of outbreaks linked to fresh produce contaminated with Shiga Toxin producing Escherichia coli (STEC). (centerforproducesafety.org)
Subsequent work will further probe the interaction of dormant STEC with growing plants. (centerforproducesafety.org)

In this study, we established RAINBOW-seq and profiled the transcriptome of Escherichia coli biofilm communities with high spatial resolution and high gene coverage. (nature.com)
To investigate the molecular mechanisms involved we determined the extent of interaction of RNA polymerase with the promoter of the tyrT stable RNA gene. (dundee.ac.uk)
Kacar then synthesized that gene and inserted it into E. coli in place of the existing version. (uncommondescent.com)

Flagella interactions with host cell surfaces were intimate and crossed cellular boundaries as demarcated by actin and membrane labelling. (ed.ac.uk)
In-cell Western assays confirmed temporal expression of flagella during O157 interaction with epithelium, early expression was suppressed during the later stages of microcolony and attaching and effacing lesion formation. (hw.ac.uk)

These mutually beneficial relationships between E. coli and humans are a type of mutualistic biological relationship - where both the humans and the E. coli are benefitting each other. (wikipedia.org)

However, little is currently known regarding the interactions between probiotics/pathogens and epithelial cell mucins. (korea.ac.kr)
This study can provide a scientific basis for revealing the interaction between food-borne pathogens and spoilage bacteria during mixed biofilm formation and for risk assessment, prevention and control. (spkx.net.cn)

Ferrous and ferric state of cytochromes P450 in intact Escherichia coli cells: a possible role of cytochrome P450-flavodoxin interactions. (nel.edu)
SWCNT-induced inflammation facilitated the recruitment of dendritic cells (DC) to the lung tissues, increasing chances of direct DC/SWCNT interactions. (cdc.gov)

E. coli stains Gram-negative because its cell wall is composed of a thin peptidoglycan layer and an outer membrane. (wikipedia.org)
The outer membrane surrounding the cell wall provides a barrier to certain antibiotics such that E. coli is not damaged by penicillin. (wikipedia.org)
Here we present the crystallographic structures of the natural siderophore-antibiotic conjugate albomycin and the siderophore phenylferricrocin, in complex with the active outer membrane transporter FhuA from Escherichia coli. (rcsb.org)

Since many pathways in mixed-acid fermentation produce hydrogen gas, these pathways require the levels of hydrogen to be low, as is the case when E. coli lives together with hydrogen-consuming organisms, such as methanogens or sulphate-reducing bacteria. (wikipedia.org)
LPS functions in bacteria-host interactions that regulate the host system responses. (sigmaaldrich.com)
The conversion of fibrinogen to fibrin at the surface of curliated E. coli bacteria leads to the generation of proinflammatory fibrinopeptides. (lu.se)
Samples containing fibrinopeptides generated by the interaction between bacteria and plasma were injected into animals and the inflammatory response was monitored. (lu.se)

Besides autodock studies, Schiffer-Edmundson helical wheel diagrams of LTB in α-helix domain suggested that LTB hydrophobic core is a highly effective region, which was able to form favorable non-polar interactions of the protein's binding surface (with amino acids residues such as Tyr, Leu, Ile) with EPS-LM. (hal.science)

She learns that contaminated spinach has been linked to an E. coli outbreak. (cdc.gov)

Although the EDP is the more thermodynamically favourable of the three pathways, E. coli do not use the EDP for glucose metabolism, relying mainly on the EMPP and the OPPP. (wikipedia.org)
Inhibitory Mechanism of Metal Antimicrobial Peptide SIF4 on Respiratory Metabolism and Energy Metabolism of Escherichia coli [J]. FOOD SCIENCE, 0, (): 0-0. (spkx.net.cn)

T. Bacillus subtilis biofilm formation and social interactions. (nature.com)

E. coli expression of TIMP-4 and comparative kinetic studies with TIMP-1 and TIMP-2: insights into the interactions of TIMPs and matrix metalloproteinase 2 (gelatinase A) . Biochemistry , 41 (50), 15025-1535. (uea.ac.uk)

Dynamical models based upon known interactions between these master genes, such as mutual antagonism and autoregulation, fail to make the system bistable, a desired feature for robust lineage determination. (lu.se)

In a bid to enhance food safety there is a need to further understand the survival, resistance and interaction of the pathogenic group with produce. (centerforproducesafety.org)

Escherichia coli (/ˌɛʃəˈrɪkiə ˈkoʊlaɪ/ ESH-ə-RIK-ee-ə KOH-ly) is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms. (wikipedia.org)
Mutualism is an interaction in which both organisms involved benefit from the relationship. (proprofs.com)

Molecular interactions of TIMPs and MMPs investigated by IAsys biosensor analysis highlighted different modes of interaction between proMMP-2-TIMP-2 (or TIMP-4) and active MMP-2-TIMP-2 (or TIMP-4) complexes. (uea.ac.uk)
In this study, the interaction of exopolysaccharides from Leuconostoc mesenteroides P35 (EPS-LM) with Escherichia coli heat-labile enterotoxin B-pentamer (LTB) was investigated at different concentrations and temperatures by using surface plasmon resonance (SPR) and molecular docking approaches. (hal.science)
The materials we make are niche polymers with tailor-made sites that facilitate selective molecular interactions. (lu.se)
The MIPs are produced by various polymerization methods but in all cases specifi c interactions, molecular partitioning and polymer structure are key properties that we focus on. (lu.se)

Protein-protein interactions (PPIs) play essential roles in many biological processes. (nature.com)

Escherichia coli, Enterobacter aerogenes, Shigella species, Acinetobacter species, Haemophilus influenzae (respiratory infections), Klebsiella species (respiratory and urinary infections). (rxlist.com)
Formation, Interaction and Novel Control Strategies of Mixed-Species Biofilm in the Food Industry [J]. FOOD SCIENCE, 2022, 43(19): 285-294. (spkx.net.cn)
The general objective of this thesis is to deepen our knowledge of the environmental drivers of the fecundity of forest trees and their interactions, by focusing on the holm oak, a widespread species in the Mediterranean basin. (cnrs.fr)

E. coli dihydrodipicolinate reductase exhibits unusual nucleotide specificity, with NADH being kinetically twice as effective as NADPH as u reductant as evidenced by their relative V/K values. (elsevierpure.com)
To investigate the nature of the interactions which determine this specificity, we performed isothermal titration calorimetry to determine the thermodynamic parameters of binding and determined the three-dimensional structures of the corresponding enzyme-nucleotide complexes. (elsevierpure.com)
In the present review, we will deal with work performed on substrate specificity, reaction mechanism and 3D structure of E. coli MurD. (degruyter.com)

3rd/4th week to combat respiratory illness due to E. coli . (cdc.gov)

This study was therefore designed to investigate the in vitro therapeutic outcome of combining an aminoglycoside (gentamicin) and some floroquinolones including ciprofloxacin, ofloxacin, perfloxacin and gatifloxacin at different ratios against E. coli isolates expressing ESBL enzymes. (scialert.net)

Escherichia coli DiaA is a DnaA-binding protein that is required for the timely initiation of chromosomal replication during the cell cycle. (elsevierpure.com)

STRING allows inspection of the interaction evidence for any given network. (string-db.org)

The revised model succeeded in fitting USAXS/SAXS and differently contrasted VSANS/SANS data of E. coli ATCC 25922 over four orders of magnitude in length scale. (iucr.org)

The present study evaluated the antimicrobial effect of different combinations of gentamicin and floroquinolones (ciprofloxacin, ofloxacin, perfloxacin and gatifloxacin) against extended spectrum beta lactamase (ESBL) positive E. coli isolates using checkerboard method. (scialert.net)

In the primary reservoir host, cattle, the terminal rectum is the principal site of E. coli O157 colonization. (hw.ac.uk)

They traced the spinach back to a California farm and matched the particular type of E. coli found in irrigation water at the farm to the E.coli in sick patients. (cdc.gov)

When we put in the ancient form, we are undoubtedly disrupting some of those interactions," Gaucher said. (uncommondescent.com)

Anatomy8

Organisms27

Diseases8

Chemicals and Drugs103

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes84

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care4

Strains11

O157:H716

Escherichia coli K121

Bacterial3

Proteins4

Organism3

Salmonella2

TRNA2

Campylobacter1

Strain2

MG16551

Bovine1

Single-stranded2

Substrates1

Protein complexes1

STEC4

Gene3

Flagella2

Humans1

Pathogens2

Cells2

Outer membrane3

Bacteria4

Residues1

Outbreak1

Metabolism2

Biofilm formation1

Insights into the interactions1

Genes1

Pathogenic1

Organisms2

Molecular4

Biological1

Species3

Specificity3

Respiratory1

Investigate1

Initiation1

Evidence1

Data1

Study1

Terminal1

Type1

Form1