Coli metabolic engineering. Medical search. Frequent questions

Bacterial cancer therapy was developed using probiotic Escherichia coli Nissle 1917 (EcN) for medical intervention of colorectal cancer. (nature.com)
In this work, a modeling approach was applied to describe the physiological behavior and the metabolic fluxes of a shikimic acid overproducing Escherichia coli strain lacking the major glucose transport system, grown on complex media. (biomedcentral.com)
Both model sets provided information that could be applied to enhance shikimic acid production on an engineered shikimic acid overproducing Escherichia coli strain. (biomedcentral.com)
In this study, an Escherichia coli K-12 MG1655 strain with reduced aromatic aldehyde reduction (RARE) that does duty for a platform for aromatic aldehyde biosynthesis was used to prompt more PAA biosynthesis. (springeropen.com)
By focusing on the biosynthesis of terrequinone A, we hope to explore the way to synthesize bis-indolylquinones de novo using Escherichia coli as a cell factory. (biomedcentral.com)
Vanillin is an industrially valuable molecule that can be produced from simple carbon sources in engineered microorganisms such as Saccharomyces cerevisiae and Escherichia coli . (biomedcentral.com)
We first functionally demonstrate the design of the MCG pathway in vitro and in Escherichia coli. (osti.gov)
Coenzyme Q production by metabolic engineered Escherichia coli strains" by Irene Martinez, Nataly Maturana et al. (engconfintl.org)
Escherichia coli produces CoQ8 naturally but it is able to produce CoQ10 when an heterologous decaprenyl synthase is expressed. (engconfintl.org)
Recombinant Escherichia coli strains have been constructed by metabolic engineering strategies to produce inositol, but with a low yield. (biomedcentral.com)
Genome-wide analysis of redox reactions reveals metabolic engineering targets for D-lactate overproduction in Escherichia coli. (leehicam.com)
This study analyzed the genome-wide anaerobic fermentation products of 472 Escherichia coli single gene knockouts, which comprised mainly of dehydrogenases, oxidoreductases, and redox-related proteins. (leehicam.com)
The biosynthesis of cyanidin 3-O-glucoside in Escherichia coli was demonstrated by means of genetic engineering. (wikipedia.org)
A newly discovered receptor in a strain of Escherichia coli can be blocked to avert infection, a finding that might aid in developing better therapies to treat bacterial infections resulting in food poisoning, diarrhea or plague. (sciencedaily.com)
In Vivo and in Vitro Synthesis of Phosphatidylglycerol by an Escherichia coli Cardiolipin Synthase. (duke.edu)
Phosphatidylglycerol (PG) makes up 5-20% of the phospholipids of Escherichia coli and is essential for growth in wild-type cells. (duke.edu)
Metabolic engineering of Escherichia coli to produce a monophosphoryl lipid A adjuvant. (duke.edu)
The gene encoding a (2 R ,3 R )-2,3-butanediol dehydrogenase from Rhodococcus erythropolis WZ010 (ReBDH) was over-expressed in Escherichia coli and the resulting recombinant ReBDH was successfully purified by Ni-affinity chromatography. (mdpi.com)
Isolation of chromosomal mutations that affect carotenoid production in Escherichia coli: mutations alter copy number of ColE1-type plasmids. (igem.org)
Enabling anaerobic growth of Escherichia coli on glycerol in defined minimal medium using acetate as redox sink. (mpg.de)
Transcriptional network response of carbon-limited Escherichia coli to transient carbon excess. (mpg.de)
The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12. (mpg.de)
Temperature-dependent dynamic control of the TCA cycle increases volumetric productivity of itaconic acid production by Escherichia coli. (mpg.de)
Model-Based metabolic engineering enables high yield itaconic acid production by Escherichia coli. (mpg.de)
Enforced ATP futile cycling increases specific productivity and yield of anaerobic lactate production in Escherichia coli. (mpg.de)
A mathematical model of metabolism and regulation provides a systems-level view of how Escherichia coli responds to oxygen. (mpg.de)
Analysis of Escherichia coli Mutants with a Linear Respiratory Chain. (mpg.de)
Thioproline formation as a driver of formaldehyde toxicity in Escherichia coli. (mpg.de)
Awakening a latent carbon fixation cycle in Escherichia coli. (mpg.de)
Activating Silent Glycolysis Bypasses in Escherichia coli. (mpg.de)
Change in cofactor specificity of oxidoreductases by adaptive evolution of an escherichia coli nadph-auxotrophic strain. (mpg.de)
For efficient production of P4HB recombinant Escherichia coli has been employed. (biomedcentral.com)
The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using an Escherichia coli genetically engineered bacterium. (justia.com)
Here we purified recombinant GsADH from Escherichia coli and determined its crystal structure. (rcsb.org)
Here, we build large, intact carboxysome shells (over 90 nm in diameter) in the industrial microorganism Escherichia coli by expressing a set of carboxysome protein-encoding genes. (nature.com)
Metabolic engineering and pathway construction for O -acetyl-L-homoserine production in Escherichia coli . (bvsalud.org)
Rerouting Fluxes of the Central Carbon Metabolism and Relieving Mechanism-Based Inactivation of l-Aspartate-α-decarboxylase for Fermentative Production of ß-Alanine in Escherichia coli . (bvsalud.org)
Escherichia coli, Saccharomyces cerevisiae and Streptomyces sp. (upm.edu.my)
We have contributed significant new gene expression and metabolite data sets for control and test fermentations in the first phase of this multi-centre 5 year project and the analyses of these data have been published showing that Escherichia coli is an excellent host for the production of organic acids. (ukri.org)
Other bacterial organisms include Streptococcus viridans, Haemophilus influenzae, Streptococcus pyogenes, and Escherichia coli . (medscape.com)

Several mathematical frameworks have been developed to understand the physiological and metabolic state of production strains and to identify genetic modification targets for improved bioproduct formation. (biomedcentral.com)
The biosynthesis of high value-added compounds using metabolically engineered strains has received wide attention in recent years. (biomedcentral.com)
Construction of metabolically engineered E. coli strains with high stoichiometric yield of inositol is desirable. (biomedcentral.com)
a series of engineered strains for inositol production was constructed by deleting the key genes pgi , pfkA and pykF . (biomedcentral.com)
The metabolically engineered E. coli strains represent a promising method for future inositol production. (biomedcentral.com)
Design and engineering of E. coli metabolic sensor strains with a wide sensitivity range for glycerate. (mpg.de)
Targeting lacZ in wild-type E. coli allows simple blue-white screening to identify edited mutant strains. (sigmaaldrich.com)
Six different E. coli strains which are able to utilize xylose as carbon source were compared for their ability to accumulate P4HB. (biomedcentral.com)

We establish a microbial biosynthetic pathway for PAA production from the simple substrate phenylalanine in E. coli with heterologous coexpression of aminotransferase (ARO8), keto acid decarboxylase (KDC) and aldehyde dehydrogenase H (AldH) gene. (springeropen.com)
In this study, we establish a microbial biosynthetic pathway for PAA production from the simple substrate phenylalanine through overexpression of an aminotransferase gene ARO8, a keto acid decarboxylase gene KDC from S. cerevisiae and an aldehyde dehydrogenase H gene aldH from E. coli in E. coli (Fig. 1 ). (springeropen.com)
Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli. (igem.org)
An optimized methanol assimilation pathway relying on promiscuous formaldehyde-condensing aldolases in E. coli. (mpg.de)
Optimizing E. coli as a formatotrophic platform for bioproduction via the reductive glycine pathway. (mpg.de)
Growth of E. coli on formate and methanol via the reductive glycine pathway. (mpg.de)
Metabolic engineering rarely succeeds at the first attempt to introduce and activate a heterologous pathway in a host organism. (biomedcentral.com)
The research team engineered the fatty acid metabolism to provide the fatty acid derivatives that are shorter than normal intracellular fatty acid metabolites, and introduced a novel synthetic pathway for the biosynthesis of short-chain alkanes. (technovelgy.com)
Strategies such as engineering a flavonoid biosynthetic pathway in microbial hosts provide an alternative way to produce these beneficial compounds. (upm.edu.my)

In recent years, with the development of synthetic biology and metabolic engineering, the biosynthesis of high-value natural products in microorganisms has emerged as a promising route to perfectly avoid the above two problems. (biomedcentral.com)
Potential topics include, but are not limited to, microbial physiology, biochemical engineering, genetic and metabolic engineering and systems biotechnology of food microorganisms. (biomedcentral.com)
Nonetheless, we are pleased to report, for the first time, the production of gasoline through the metabolic engineering of E. coli, which we hope will serve as a basis for the metabolic engineering of microorganisms to produce fuels and chemicals from renewable resources. (technovelgy.com)
With the aim of producing important chemicals in microorganisms, we focus our efforts at investigating novel biochemical pathways and engineering microorganisms to host these pathways. (lbl.gov)

Engineering lactic acid bacteria (LAB) is of growing importance for food and feed industry as well as for in vivo vaccination or the production of recombinant proteins in food grade organisms. (biomedcentral.com)
The biosynthesis of inositol from glucose in recombinant E. coli was optimized by metabolic engineering strategies. (biomedcentral.com)
The effect of growth conditions such as temperature and physiological stage of Na-4HB addition on P4HB synthesis was also studied in E. coli JM109 recombinant in batch culture. (biomedcentral.com)
are among the expression systems used to produce recombinant products, as well as for the production of flavonoid compounds through various bioengineering approaches including clustered regularly interspaced short palindromic repeats (CRISPR)-based genome engineering and genetically encoded biosensors to detect flavonoid biosynthesis. (upm.edu.my)

Many metabolic pathways that were located far from anaerobic mixed-acid fermentation significantly affected the profiles of lactic acid, succinic acid, acetic acid, formic acid, and ethanol. (leehicam.com)
Underground isoleucine biosynthesis pathways in E. coli. (mpg.de)
Specifically, we aim to 1) engineer organisms that are uniquely tolerant to reaction conditions, 2) use protein engineering and synthetic biology tools to enhance enzyme production and regulation in these hosts, and 3) discover novel pathways and organisms through bioprospecting to expand the range of chemicals to be synthesized and traits to be selected from. (aiche.org)
A Unique Bioengineering Platform for the Efficient Optimization of Metabolic Pathways- Kedar Patel, DNA2.0 Inc. (aiche.org)
Our work in biochemistry and microbiology focuses on understanding unknown metabolic pathways, enzymes or microbial processes that will be central to be able to engineer new metabolic functions inside cells. (lbl.gov)

Moreover, it introduces the capability to use 13 C labeling experimental data to constrain comprehensive genome-scale models through a technique called two-scale 13 C Metabolic Flux Analysis (2S- 13 C MFA). (biomedcentral.com)
CRISPR-Cas systems are a new generation of RNA-guided microbial genome engineering tools that allow targeted genome modifications, such as insertions, deletions, and point mutations, to be introduced with greater ease than ever before. (sigmaaldrich.com)

Among different modeling schemes, the quantification of intracellular metabolic fluxes (i.e. the rate of each reaction in cellular metabolism) is of particular interest for metabolic engineering because it describes how carbon and energy flow throughout the cell. (biomedcentral.com)
The jQMM library presented here provides an open-source, Python-based framework for modeling internal metabolic fluxes and leveraging other -omics data for the scientific study of cellular metabolism and bioengineering purposes. (biomedcentral.com)

This study provides a metabolic engineering strategy for the efficient synthesis of terrequinone A and other similar bis-indolylquinones with asymmetric quinone cores. (biomedcentral.com)

In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template. (sigmaaldrich.com)
We use this tool to perform multi-gene editing in E. coli for production of a clinically relevant compound known as Kdo2-lipid A. (sigmaaldrich.com)
In addition to conventional metabolic engineering to increase biochemical production, we will develop new tools to enhance enzyme expression and create gene knockouts in non-model organisms. (aiche.org)
In the study, glycerol uptake facilitator or aquaglyceroporin gene (glpF) and glycerol kinase (glpK) gene were PCR-cloned from E. coli, inserted into a shuttle vector pBS29P2gfp, and expressed in P. chlororaphis by a Pseudomonas promoter P2. (usda.gov)

This study reports the successful genetic engineering (GE) of the Pseudomonas chlororaphis to result in improved strain capable of consuming the glycerol substrate at a faster rate, thus cutting down on the production time and therefore making the bioprocess more cost-friendly. (usda.gov)
The objective of this study is to improve by genetic engineering the glycerol metabolic capability of Pseudomonas chlororaphis which is capable of producing commercially valuable biodegradable poly(hydroxyalkanoate) (PHA) and biosurfactant rhamnolipids (RLs). (usda.gov)

By administration locally or systemically, the engineered bacteria target tumors where they reside, replicate, and continuously produce the payloads on site. (nature.com)
E. coli , one of the most commonly engineered bacteria, has an active homologous recombination (HR) system, so chromosomal integration can be achieved simply by donating DNAs with homology arms to recombine at a target site. (sigmaaldrich.com)

Classic metabolic engineering strategies often induce significant flux imbalances to microbial metabolism, causing undesirable outcomes such as suboptimal conversion of substrates to products. (biomedcentral.com)
Although classic metabolic engineering (ME) allows flux redirection in a biochemical network into valuable compounds by genetic manipulation, it often induces significant flux imbalances to the CCM that may cause undesirable outcomes. (biomedcentral.com)
Thus, the cellular metabolic flux can be controlled either by modulation of the electron flow or the regulation of redox reactions. (leehicam.com)
Firstly, it presents a complete toolbox for simultaneously performing two different types of flux analysis that are typically disjoint: Flux Balance Analysis and 13 C Metabolic Flux Analysis. (biomedcentral.com)
Combinatorial libraries and metabolic flux analysis will be used to balance enzyme expression. (aiche.org)
The titer is rather low due to the low metabolic flux towards the formation of short-chain fatty acids and their derivatives. (technovelgy.com)

Therefore, there is an increasing interest into a more global and detailed understanding of the metabolic and regulatory network changes imposed by different genetic modifications or process conditions in various production systems. (biomedcentral.com)
2016.Engineering of a nepetalactol-producing platform strain of Saccharomycescerevisiae for the production of seco-iridoids. (concordia.ca)
Improvement of Acetaldehyde Production in Zymomonas mobilis by Engineering of Its Aerobic Metabolism. (mpg.de)
Understanding of metabolism is also of central importance to the microbial production of drugs, bioproducts and biofuels through metabolic engineering [ 5 ]. (biomedcentral.com)
7 In this article, we used our novel E. coli CRISPR λ-Red HR system to engineer an industrial E. coli strain with enhanced production of a valuable biomedical research compound known as Kdo2-lipid A. (sigmaaldrich.com)
In the paper (entitled "Microbial Production of Short-chain Alkanes") published online in Nature on September 29, a Korean research team led by Distinguished Professor Sang Yup Lee of the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology (KAIST) reported, for the first time, the development of a novel strategy for microbial gasoline production through metabolic engineering of E. coli. (technovelgy.com)
These findings serve as a practical knowledge to enhance B. subtilis 168 butanol tolerance, and demonstrate means to engineer the bacterial host to promote higher butanol/alcohol tolerance of B. subtilis 168 for the production of butanol and other alcohol biocommodities. (biomedcentral.com)
We have also published the results of research to enhance the tolerance of E. coli to vanillin and have identified several potential intervention points to improve vanillin production. (ukri.org)
2006. "Production of the antimalarial drug precursor artemisinic acid in engineered yeast. (lbl.gov)
2015. "Comprehensive structural analysis of the terminal myxalamid reductase domain for the engineered production of primary alcohols. (lbl.gov)

Using a combination of functional genomics, metabolic engineering and synthetic biology techniques, we study microbes normally found in the environment in order to better understand their metabolism and physiology with the purpose of eventually engineering them as alternatives to producing, cleaner, better, cheaper or even novel products. (concordia.ca)
jQMM will facilitate the design and metabolic engineering of organisms for biofuels and other chemicals, as well as investigations of cellular metabolism and leveraging -omics data. (biomedcentral.com)

Synthetic Methanol and Formate Assimilation Via Modular Engineering and Selection Strategies. (mpg.de)
We will combine knowledge of these systems to develop our DeTox strain platform by strain engineering, using synthetic biology standards. (ukri.org)

2015. Metabolic engineering of atyrosine-overproducing yeast platform strain evaluated by targetedmetabolomics. (concordia.ca)

Researchers at UT Southwestern Medical Center are the first to identify the receptor, known as QseC, used by a diarrhea-causing strain of E coli to receive signals from human flora and hormones in the intestine and express virulence genes to initiate infection. (sciencedaily.com)

E. coli is easy to culture and relatively easy to modify genetically which makes it suitable for the development of an industrial-scale process. (engconfintl.org)

Workplace exposure assessment of BNNT from two manufacturing facilities measured boron concentrations in personal breathing zones from non-detectable to 0.95g/m3 and TEM structure counts of 0.0123 0.0094 structures/cm3, concentrations well below what was found with other engineered high aspect ratio nanomaterials like carbon nanotubes and nanofibers. (cdc.gov)

Product toxicity and removal are two long-standing challenges to bioprocess engineering. (aiche.org)
We will study the mechanisms of chemical toxicity and resistance in E. coli & solventogenic Clostridium spp. (ukri.org)

All three quinone species play distinct roles in ensuring optimal growth under aerobic and fermentative conditions in E. coli K 12. (mpg.de)
Ribulose Monophosphate Shunt Provides Nearly All Biomass and Energy Required for Growth of E. coli. (mpg.de)
E. coli JM109 was found to be the best strain regarding the specific growth rate and the P4HB content. (biomedcentral.com)
Metabolic engineering of microbes has primarily focused on modifying the model organisms E. coli and S. cerevisiae due to their fast and robust growth on inexpensive fermentation broths and the wealth of genetic tools available to them. (aiche.org)

Inositol was successfully biosynthesized after introducing two crucial enzymes: inositol-3-phosphate synthase (IPS) from Trypanosoma brucei , and inositol monophosphatase (IMP) from E. coli . (biomedcentral.com)
The naturally designed architecture, semi-permeability, and catalytic improvement of carboxysomes have inspired rational design and engineering of new nanomaterials to incorporate desired enzymes into the protein shell for enhanced catalytic performance. (nature.com)
The study provides insights into the assembly and formation of carboxysomes and paves the way for engineering carboxysome shell-based nanoreactors to recruit specific enzymes for diverse catalytic reactions. (nature.com)
This represents a step towards synthetically engineering large and stable nanoreactors that are capable of sequestering high copy numbers of cargo enzymes and providing selective permeability to substrates to boost enzyme activities. (nature.com)

Nevertheless, the accumulation of intracellular proline against butanol stress required a metabolic conversion of glutamate through the specific biosynthetic ProB-ProA-ProI route. (biomedcentral.com)

These results suggest that the anaerobic fermentation profiles of E. coli can be tuned via the disruption of peripheral dehydrogenases in anaerobic conditions. (leehicam.com)

On the flexibility of the cellular amination network in E. coli. (mpg.de)

An "energy-auxotroph" E. coli provides an in vivo platform for assessing NADH regeneration systems. (mpg.de)
Our data clarified the substrate scope of GsADH and provided structural insights, which may facilitate more efficient cofactor regeneration and rational metabolic engineering. (rcsb.org)

Schematic of Dual-Vector E. coli CRISPR λ-Red HR System. (sigmaaldrich.com)

Aducanumab (brand name Aduhelm) is a monoclonal antibody engineered in a laboratory to stick to the amyloid molecule that forms plaques in the brains of people with Alzheimer's. (curiouscatblog.net)

In addition, this is the first report on the de novo biosyhthesis of terrequinone A in an engineered strain. (biomedcentral.com)

Metabolic analysis of L-Trp indicated that the conversion of large amounts of L-Trp to indole was an important factor preventing the further improvement of terrequinone A yield. (biomedcentral.com)

In this study, we review the recent advances in engineering model microbial hosts as being the factory to produce targeted flavonoid compounds. (upm.edu.my)

This issue was addressed by metabolic engineering of EcN to uncouple the glucose-mediated control circuit and the L-arabinose transport-induction loop and to block L-arabinose catabolism. (nature.com)

Wison Engineering Services Co. Ltd., together with its subsidiaries, announced that Wison Engineering Ltd., an indirect non-wholly owned subsidiary of the Group, has been awarded a contract for the engineering of Erdos Jinchengtai coal-to-methanol Project (Phase II) for Erdos Jinchengtai Chemical Co., Ltd. Wison Engineering will partner with Erdos Jinchengtai. (greencarcongress.com)

Multienzyme polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) are molecular assembly lines that biosynthesize complex natural products, making them ideal for rational enzyme engineering. (lbl.gov)

This allowed the development of platform E. coli strain capable of producing gasoline for the first time. (technovelgy.com)

1 Recombineering is a popular method that utilizes phage-derived proteins, the Lambda-Red (λ-Red) system, to increase the efficiency of recombination in E. coli . (sigmaaldrich.com)

Anatomy3

Organisms28

Diseases1

Chemicals and Drugs70

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes46

Disciplines and Occupations8

Technology, Industry, Agriculture18

Information Science7

Health Care4

Escherichia40

Strains8

Pathway9

Microorganisms4

Recombinant4

Pathways5

Genome2

Fluxes2

Synthesis1

Gene4

Pseudomonas2

Bacteria2

Flux6

Production10

Metabolism2

Synthetic2

Yeast1

Genes1

Genetically1

Carbon1

Toxicity2

Growth4

Enzymes4

Intracellular1

Anaerobic1

Cellular1

Regeneration2

CRISPR1

Laboratory1

Novo1

Analysis1

Compounds1

Glucose1

Methanol1

Rational1

Strain capable1

Proteins1