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  • periplasmic
  • To study peptide import in E. coli I have characterized the in C41(DE3) strongly up regulated periplasmic binding protein OppA using a combination of biochemical and structural methods. (avhandlingar.se)
  • FtsI, FtsL, and FtsQ are not homologous but have similar overall structures: a small cytoplasmic domain, a single membrane-spanning segment (MSS), and a large periplasmic domain that probably encodes the primary functional activities of these proteins. (asm.org)
  • Before LPS is assembled at the OM, it must be synthesized at the inner membrane (IM) and transported across the aqueous periplasmic compartment. (pnas.org)
  • OM lipoproteins interact with the ABC transporter LolCDE, which extracts them from the IM and passes these proteins to the periplasmic chaperone LolA for delivery to LolB at the OM ( 4 ). (pnas.org)
  • Porin
  • Porin proteins in the OM control permeability to hydrophilic molecules, but unlike typical phospholipid bilayers, the OM is quite impermeable to hydrophobic molecules, mainly because of LPS ( 1 ). (pnas.org)
  • OmpF
  • This study was undertaken to investigate the proposed in vivo pore function of PhoE protein, an Escherichia coil KI2 outer membrane protein induced by growth under phosphate limitation, and to compare it with those of the constitutive pore proteins OmpF and OmpC. (uu.nl)
  • PhoE, OmpF or OmpC, or none of these proteins at all. (uu.nl)
  • Comparison of the rates of permeation of Pi, glycerol 3-phosphate and glucose 6-phosphate through pores formed by PhoE, OmpF and OmpC proteins shows that PhoE protein is the most effective pore in facilitating the diffusion of Pi and phosphorus-containing compounds. (uu.nl)
  • DedA
  • Multiple deletions reveal the essentiality of the DedA membrane protein family in Escherichia coli. (nih.gov)
  • The DedA family is a highly conserved, ancient family of membrane proteins with representatives in most sequenced genomes. (nih.gov)
  • These results indicate that while not individually essential, the E. coli DedA family proteins are collectively essential. (nih.gov)
  • These observations suggest important functions for the E. coli DedA protein family. (nih.gov)
  • The aim of this dissertation is to characterize the physiological roles of E. coli YqjA and YghB, which belong to the ancient and conserved DedA protein family of hitherto unknown functions. (plu.mx)
  • mutant
  • Depletion of YhcB, an inner membrane protein of Escherichia coli , inhibited the growth of rodZ deletion mutant showing that the loss of both YhcB and RodZ is synthetically lethal. (hindawi.com)
  • predominance of green indicates that the corresponding protein spot is more abundant in wild type than mutant, and predominance of magenta indicates the opposite. (nih.gov)
  • By measuring rates of nutrient uptake at low solute concentrations, the proposed pore function of PhoE protein was confirmed as the presence of the protein facilitates the diffusion of Pi through the outer membrane, such that a pore protein deficient strain behaves as a Km mutant. (uu.nl)
  • structural
  • Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. (avhandlingar.se)
  • Structural biology is increasingly reliant on elevated throughput methods for protein production. (chalmers.se)
  • Membrane proteins are extremely challenging to produce in sufficient quantities for biochemical and structural analysis and there is a growing demand for solutions to this problem. (scilifelab.se)
  • cytoplasmic
  • To ask whether the cytoplasmic domain and MSS of each protein serve only as a membrane anchor or have instead a more sophisticated function, we have used molecular genetic techniques to swap these domains among the three Fts proteins and one membrane protein not involved in cell division, MalF. (asm.org)
  • Expression
  • Unfortunately, over-expression of membrane proteins in E. coli is usually toxic to the cells. (avhandlingar.se)
  • During my Ph.D. I have tried to understand the reasons for this toxicity by studying the consequences of membrane protein over-expression using a combination of proteomics and more focused biochemical and genetic methods. (avhandlingar.se)
  • The latter two were isolated for their improved membrane protein over-expression characteristics. (avhandlingar.se)
  • With the help of this plasmid the activity of the T7RNAP can be precisely set thereby avoiding saturation of the Sec translocon upon membrane protein over-expression. (avhandlingar.se)
  • In addition, an auto-induction protocol for membrane protein expression was designed to enhance the overproduction efficiency such that, regardless of the construct used, much higher expression was achieved when compared with standard induction approaches such as isopropyl-β-d-thiogalactopyranoside (IPTG). (chalmers.se)
  • In this study we attempted to improve expression of two difficult-to-express coding sequences (araH and narK) for membrane transporters. (scilifelab.se)
  • We conclude that coding sequences can be re-wired for high-level protein expression by selective engineering of the 5' coding sequence with synonymous codons, thus circumventing the need to consider whole sequence optimization. (scilifelab.se)
  • envelopes
  • All numbered and labeled protein spots were identified using LC/MS/MS and are listed in ( E ). Isolation of envelopes and enrichment of integral membrane proteins were carried out as described in the Experimental procedures' section. (nih.gov)
  • essential
  • Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. (avhandlingar.se)
  • We discuss several potential interpretations of these results, including that the essential domains of FtsI, FtsL, and FtsQ have a role in regulating the localization and/or activity of these proteins to ensure that septum formation occurs at the right place in the cell and at the right time during the division cycle. (asm.org)
  • This led to the discovery of two essential Escherichia coli IM proteins of unknown function, YjgP and YjgQ, which are required for the transport of LPS to the cell surface. (pnas.org)
  • respectively
  • agn43, which is present in a single copy within E. coli K-12, is subject to phasevariable regulation where OxyR and Dam methylase function as repressor and derepressor, respectively. (tcd.ie)
  • FUNCTION
  • Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. (uniprot.org)
  • PROTEIN SEQUENCE OF 23-34 , FUNCTION, SUBCELLULAR LOCATION. (uniprot.org)
  • In E. coli , the main function of the OM is to serve as a selective permeability barrier against many toxic chemicals, such as detergents and antibiotics ( 1 ). (pnas.org)
  • Crystallization
  • The membrane protein-antibody fragment complex may enter crystallization trials with the antibody fragment driving the complex nucleation through the formation of numerous, strong crystal contacts. (gatech.edu)
  • Such a co-crystallization method with anti-EE scFv and Fab fragments provides the protein crystallographer with a "crystallization toolbox" that can be used for any crystallographic study of a protein of interest. (gatech.edu)
  • Through size exclusion chromatography and SDS-PAGE analysis, complexation of the β-barrel membrane protein intimin harboring the EE tag with scFv/EE and Fab/EE has been shown to occur, from which crystallization trials have ensued. (gatech.edu)
  • cultures
  • Protein production was performed in 0.5 ml cultures in standard 24-well plates, allowing increased throughput with minimal effort. (chalmers.se)
  • Small-volume cultures (10 mL) produced sufficient amount of proteins to assess their purity and aggregation state. (univ-grenoble-alpes.fr)