Coli caulobacter. Medical search. Frequent questions

EcoDam from E. coli and CcrM from Caulobacter crescentus are well characterized members of these family. (wikipedia.org)
The dimorphic bacterium Caulobacter crescentus is a model organism for studying the bacterial cell cycle. (csun.edu)
Transposon mutagenesis paired with deep sequencing of Caulobacter crescentus under uranium stress reveals genes essential for detoxification and stress tolerance. (csun.edu)
p)ppGpp modulates cell size and the initiation of DNA replication in Caulobacter crescentus in response to a block in lipid biosynthesis. (csun.edu)
2012. The Caulobacter crescentus ctrA P1 promoter is essential for the coordination of cell cycle events that prevent the overinitiation of DNA replication. (csun.edu)
Sequence comparison between wild-type hfsH from Caulobacter crescentus [37] and our optimized gene for Escherichia coli . (igem.org)
Sequence comparison between wild-type hfsG from Caulobacter crescentus [37] and our optimized gene for Escherichia coli . (igem.org)
Caulobacter crescentus dividing into a stalk daughter cell (top) and a motile daughter cell with a flagellum (bottom). (kenyon.edu)
Caulobacter crescentus is a single-celled, small bacterium that divides asymmetrically producing cells that differ in structure and function - it is an ideal model system for the mechanisms of asymmetric cell division and has been studied thoroughly. (kenyon.edu)
The genome of Caulobacter crescentus is structured in a circular chromosome and is comprised of 4,016,942 bp encoding for 3,767 genes. (kenyon.edu)
As a model system, they exploited the gram-negative, alpha-proteobacterium Caulobacter crescentus, which is widely present in fresh-water lakes and streams. (asbmb.org)
Current research examines the general principles and spatiotemporal mechanisms by which bacterial cells replicate, using Caulobacter crescentus and Escherichia coli as models. (stanford.edu)
The creation of a reporter that uses the urcA (uranium response in Caulobacter crescentus) promoter to produce a UV-excitable green fluorescent protein in the presence of the uranyl cation is described. (readabstracts.com)
We have solved the X-ray crystal structure of the RNA chaperone protein Hfq from the alpha-proteobacterium Caulobacter crescentus to 2.15-Å resolution, resolving the conserved core of the protein and the entire C-terminal domain (CTD). (johnshopkins.edu)
We also show that C. crescentus Hfq has sRNA binding and RNA annealing activities and is capable of facilitating the annealing of certain Escherichia coli sRNA:mRNA pairs in vivo. (johnshopkins.edu)
Segregation of chromosomes and plasmids is achieved while they are being replicated by using the Par system in some bacteria such as the budding bacteria Caulobacter crescentus , as illustrated in Figure 2.1 . (hideyuki-ts.com)
Figure 2.1: The Par system segregates replicated chromosomes in Caulobacter crescentus. (hideyuki-ts.com)
Our primary model organisms are Caulobacter crescentus, Escherichia coli and the Lyme disease pathogen Borrelia. (berkeley.edu)
evolved Caulobacter crescentus for 2000 generations under conditions where selection was strong early in life, but weak late in life. (science20.com)
Using both native microbial features as well as advanced bioengineering technologies, we engineered Escherichia coli/Caulobacter crescentus for high-density cell surface displays of lanthanide-binding tags, peptides that exhibit high affinity and selectivity for REEs. (llnl.gov)
For this purpose, they have given it the special ability of another bacterium: of Caulobacter crescentus ( see here ). (fz-juelich.de)
The researchers focused on type II topoisomerases found in a common species of freshwater bacteria, Caulobacter crescentus . (innovitaresearch.com)
Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. (unl.edu)

Caulobacter in contrast, as an alpha-proteobacteria, is closely related to human pathogenic bacteria, such as Brucella and Rickettsia . (csun.edu)
In order to understand the protein factors involved in compacting Caulobacter DNA - regulating topoisomerase activity specifically - the researchers screened their bacteria for proteins that bound tightly to supercoiled DNA. (innovitaresearch.com)
On the other hand, the toxic gene (ccdB) is introduced in the E. coli chromosome of the bacteria. (igem.org)
So far research on spatiotemporal coordination of morphology and cell division has mainly focused on rod-shaped bacteria like Escherichia coli and Bacillus subtilis. (uni-marburg.de)

Characterization of an Escherichia coli O157:H7 O-antigen deletion mutant and effect of the deletion on bacterial persistence in the mouse intestine and colonization at the bovine terminal rectal mucosa. (uidaho.edu)
Thermoregulation of the Escherichia coli O157:H7 pO157 ecf operon and lipid A myristoyl transferase activity involves intrinsically curved DNA. (uidaho.edu)
Escherichia coli O157:H7 str. (up.ac.za)

Chromosome replication is principally regulated by DnaA in E. coli . (hideyuki-ts.com)
The mechanism of chromosome segregation in E. coli , which lacks Par, is not well characterised. (hideyuki-ts.com)

The modes of bacterial cell division vary, but the most common one is binary fission (Stahl 2019) , and its mechanism, especially in rod-shaped model organisms Escherichia coli and Bacillus subtilis , has been studied extensively. (hideyuki-ts.com)

In order to analyze the cell cycle regulatory mechanisms and other mechanisms of Caulobacter , many mutant strains have been made and studied. (kenyon.edu)

Most previous studies on bacterial lipid metabolism have focused on E. coli , a gamma-proteobacteria. (csun.edu)

E. coli can cause urinary tract infections, neonatal meningitis, and many different intestinal diseases, usually by attaching to the host cell and introducing toxins that disrupt normal cellular processes. (up.ac.za)

Although researchers working in E. coli and other, higher organisms have pinpointed additional proteins that can activate or repress these enzymes, none of these proteins were required for replication. (innovitaresearch.com)

This enteric organism is typically present in the lower intestine of humans, where it is the dominant facultative anaerobe present, but it is only one minor constituent of the complete intestinal microflora.E. coli, is capable of causing various diseases in its host, especially when they acquire virulence traits. (up.ac.za)

b ) A set of annotated bright-field images of Escherichia coli and Myxococcus xanthus along with synthetic labeled data with additive Gaussian noise was used to generate a training dataset of input images, X, consisting of Shape Index Map of intensity images (at three scales) and segmented images, Y, consisting of contours ( Y1 ) and cell body ( Y2 ). (elifesciences.org)

As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. (unl.edu)

Lack of this nutrient induce Caulobacter to dramatically elongate its stalk up to 30 times longer than those in phosphate-rich medium (Brun et al. (kenyon.edu)

Caulobacter also has several genes and clusters of genes that are essential for survival in habitats with poor nutrients. (kenyon.edu)

Caulobacter also uses DNA methylation as a regulatory mechanism in cell cycle progression. (kenyon.edu)
In the present review, we will deal with work performed on substrate specificity, reaction mechanism and 3D structure of E. coli MurD. (degruyter.com)

Ribose-phosphate pyrophosphokinase (prs) is a recombinant protein expressed in E. coli. (phosphokinase.com)

a ) MiSiC masks and corresponding annotated masks of fluorescence, phase contrast and bright field images of a dense E. coli microcolony. (elifesciences.org)
E. coli IoU over threshold - Phase contrast. (elifesciences.org)

As shown in Figure 3.1 , this is typically achieved using two mechanisms, nucleoid occlusion (NO), and the Min system, which are well characterised in E. coli and B. subtilis . (hideyuki-ts.com)

As a postdoctoral fellow, he joined the lab of Nobel laureate Jacques Monod at the Pasteur Institute in Paris where he studied the effect of genetic mutation in E. coli. (asbmb.org)

Caulobacter are Gram-negative, rod-like cells that can be flagellated in a polar manner or have a stalk. (kenyon.edu)
Caulobacter asymmetrically divides to produce two types of daughter cells that are functionally and structurally different. (kenyon.edu)
The cell cycle of Caulobacter showing the two daughter cells, one with a stalk and one with a flagellum, that are different in function and structure. (kenyon.edu)
Eric Stewart of Northeastern University, and his colleagues took fluorescent images of individual E. coli cells over ten generations. (science20.com)
Each generation the E. coli cells divide down the middle, giving each daughter cell one new tip and an old tip from its mother, or grandmother, or some older ancestor. (science20.com)

Escherichia coli MurD has been the first Mur ligase for which the tridimensional (3D) structure was solved. (degruyter.com)

Caulobacter asymmetrically divides to produce a motile swarmer cell and a stalk cell. (kenyon.edu)

Newton started his independent research career in 1966 as an assistant professor at Princeton where he continued his work on E. coli. (asbmb.org)

Caulobacter generally live in a dilute aquatic environment where the most common limiting nutrient is phosphorus, an essential element for healthy growth. (kenyon.edu)

This view was cemented further by the discovery of a limited degree of aging in the model bacterium Escherichia coli by Stewart and co-workers in 2005 [ 16 ]. (biomedcentral.com)
One of the first model systems for molecular biology was the bacterium Escherichia coli , a common constituent of the human digestive system. (wikidoc.org)
Escherichia coli ( E. coli ) - This common gut bacterium is the most widely-used organism in molecular genetics . (wikidoc.org)

To evaluate whether the evolution of specialists or generalists occurs under environmental fluctuation regimes with different levels of periodicity, 24 populations of Escherichia coli underwent laboratory evolution with temperatures alternating between 15 and 43°C in three fluctuation regimes: two periodic regimes dependent on culture's cell density and one random (non-periodic) regime with no such dependency, serving as a control. (frontiersin.org)
Escherichia coli has a natural system of chemotaxis ( Appendix ). (igem.org)
It is typically 20kDa and a heterodimer (e.g. in Escherichia coli, comprised of HUα and HUβ). (biologyonline.com)
Genes on a Wire: The Nucleoid-Associated Protein HU Insulates Transcription Units in Escherichia coli. (biologyonline.com)
To date, only two cell division interactomes have been characterized, in Escherichia coli and in Streptococcus pneumoniae . (biomedcentral.com)
In Escherichia coli (Ec), normal cell division is driven by the formation of an FtsZ-ring at the division site [ 1 ], followed by the recruitment of other essential proteins, which together form the divisome [ 2 ]. (biomedcentral.com)
Both ambient temperature and the DnaK chaperone machine modulate the heat shock response in Escherichia coli by regulating the switch between σ 70 and σ 32 factors assembled with RNA polymerase. (microbiologyresearch.org)
Development of BioBrick parts relies heavily on the use of a set of plasmid vectors that are only replicative in Escherichia coli and the enterics. (igem.org)
In Vivo and in Vitro Synthesis of Phosphatidylglycerol by an Escherichia coli Cardiolipin Synthase. (duke.edu)
Phosphatidylglycerol (PG) makes up 5-20% of the phospholipids of Escherichia coli and is essential for growth in wild-type cells. (duke.edu)
Escherichia coli O157:H7 str. (ucc.ie)
As part of these investigations, in early 1960, Kohiyama started to isolate mutants of Escherichia coli K12 that fail to grow at a high temperature. (encyclopedia.pub)
Escherichia coli O55:H7 str. (up.ac.za)
the carboxy-terminal domain of the transcription factor escherichia coli nusa, nusactd, interacts with the protein n of bacteriophage lambda, lambdan, and the carboxyl terminus of the e. coli rna polymerase alpha subunit, alphactd. (liverpool.ac.uk)
functional analysis of the lysis genes of staphylococcus aureus phage p68 in escherichia coli. (liverpool.ac.uk)
two-stage continuous operation of recombinant escherichia coli using the bacteriophage lambda q- vector. (liverpool.ac.uk)
a two-stage continuous culture of escherichia coli in combination with a bacteriophage lambda system was performed in order to overcome the intrinsic plasmid instability that is frequently observed in recombinant fermentation. (liverpool.ac.uk)
heterologous escherichia coli or bacteriophage lambda dna fragments of various sizes were inserted into vectors based on either the rolling-circle-type plasmid pwv01 or the theta-type plasmid pambeta1. (liverpool.ac.uk)
gene-specific random mutagenesis of escherichia coli in vivo: isolation of temperature-sensitive mutations in the acyl carrier protein of fatty acid synthesis. (liverpool.ac.uk)
moreover, recent data indicate that the acyl carrier protein of escherichia coli has a large protein interaction network that extends beyond lipid synthesis. (liverpool.ac.uk)
Our research covers cell envelope topics in in model bacteria, for example Escherichia coli , and many other bacteria, including important pathogens, bacteria living in the environment and biotechnologically important bacteria. (edu.au)

Most rod-shaped bacteria, including E. coli and B. subtilis , grow by inserting new cell-wall material into their lateral sidewalls 8 . (nature.com)
Short as well as long versions of DnaX had been observed 20 years ago in another bacteria, E. coli . (sciencedaily.com)
That RNA sequence is absent in many bacteria DnaX genes including in Caulobacter, so scientists long thought that short DnaX only existed in bacteria like E. coli . (sciencedaily.com)
Molecular characterization of Cada2 reveals a novel domain organization and DNA binding region that is distinct from its E. coli counterpart (Ada), and conserved in all bacteria encoding a Cada2-like homolog instead. (dnarepairlab.com)

Specific enzymes (glycosyltransferases, GTs), ubiquitous in all domains of life, execute an enzymatic reaction, transferring either simple monosaccharides or polysaccharides en bloc via covalent linkage to OH groups (O-glycosylation on serine or threonine residues) or to NH2-groups (N-glycosylation on asparagine) by O-GTs or N-GTs, respectively. (unige.ch)

Several of the bacterial viruses ( bacteriophage ) that infect E. coli also have been very useful for the study of gene structure and gene regulation (e.g. phages Lambda and T4 ). (wikidoc.org)

Comparisons of the N. gonorrhoeae cell division interactome with the published interactomes from E. coli and S. pneumoniae indicated that FtsA-FtsZ and FtsZ-FtsK interactions were common to all three species. (biomedcentral.com)

In work published this month in Proceedings of the National Academy of Sciences, Chien and Vass report that one of these specialized replication factors, DnaX, is, to their surprise, partially digested or trimmed, physically cut into shorter fragments, by an energy-dependent protease known as ClpXP, which generates specific-sized fragments that are essential for Caulobacter 's normal growth. (sciencedaily.com)

They showed that while cell division in E. coli was morphologically symmetric, it was not functionally symmetric: the old-pole cell, i.e. the daughter cell inheriting the older pole of the mother cell, grew more slowly and the new-pole cell grew faster than the mother cell, suggesting that the older pole was associated with more damage. (biomedcentral.com)

E. coli minicells in a ∆min strain. (asmblog.org)
Constructs will be available in derivatives of pUC18-Sfi, (high copy number, replicative in any E. coli strain), or derivatives of pUC18-R6KT (medium copy number, replicative only in E. coli strains expressing the pir gene). (igem.org)

Bacterial O-GTs are attractive biotechnological tools because of their apparent simplicity in design, small molecular mass and amenability for bacterial (E. coli-based) protein overexpression systems. (unige.ch)

E. coli has 18 known periplasmic autolysins (PG hydrolases) active during growth and cell division, and at least 6 PG carboxypeptidases for the regulation of PG synthesis. (edu.au)

For this model, equations representing reaction schemes that consider protein concentration, diffusion rates, and membrane binding/dissociation of MinD and MinE were solved for a cylindrical domain with hemispherical caps, a proxy for the shape of an E. coli cell. (asmblog.org)
While the SOS response is ubiquitously present, conservation and relevance of the adaptive response beyond the well-studied E. coli model remains less appreciated. (dnarepairlab.com)

E. coli as a host. (igem.org)

the e. coli nusa carboxy-terminal domains are structurally similar and show specific rnap- and lambdan interaction. (liverpool.ac.uk)

Simulations for a typical sized E. coli cell are shown in Figure 3. (asmblog.org)
Figure 2 shows an E. coli cell that lysed by the action of Penicillin. (edu.au)

Here, we discover a highly conserved but previously uncharacterized transcription factor (Cada2), that regulates a methylation-inducible adaptive response in Caulobacter. (dnarepairlab.com)
Thus, despite diverse organizations and mechanisms of regulation, widespread occurrence of adaptive response regulators such as Ada (E. coli-like) and Cada2 (Caulobacter-like) underscores the importance of a methylation-specific bacterial DNA damage response. (dnarepairlab.com)

But in E. coli , the short form was produced by changes in translation due to an early ribosome stop at a specific RNA sequence. (sciencedaily.com)

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Organisms14

Diseases1

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes33

Disciplines and Occupations1

Information Science4

Health Care4

Crescentus23

Bacteria4

Escherichia co3

Chromosome2

Bacillus1

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Bacterial1

Urinary Tract Infe1

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Myxococcus1

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Genetic1

Cells5

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Bacterium3

Escherichia Coli21

Bacteria4

Glycosylation1

Bacteriophage1

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