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Escherichia coli ProteinsEscherichia coliEscherichia coli InfectionsMammalsEscherichia coli O157Amino Acid SequenceBase SequenceMolecular Sequence DataCloning, MolecularBacterial ProteinsGenes, BacterialPlasmidsMutationEscherichia coli K12DNA, BacterialSequence Homology, Amino AcidAdhesins, Escherichia coliRecombinant ProteinsKineticsCell LineGene Expression Regulation, BacterialCampylobacter coliProtein BindingBinding SitesSequence AlignmentSpecies SpecificityTranscription, GeneticRecombinant Fusion ProteinsCarrier ProteinsOperonSubstrate SpecificityEmbryo, MammalianGenesGenetic Complementation TestMembrane ProteinsElectrophoresis, Polyacrylamide GelCattleRestriction MappingHeLa CellsRNA, MessengerDNATOR Serine-Threonine KinasesRNA, BacterialProtein Structure, TertiaryChromosomes, BacterialCricetinaeBacterial Outer Membrane ProteinsTranscription FactorsDNA-Binding ProteinsDNA PrimersPhylogenyGene ExpressionMolecular WeightTemperatureDNA, ComplementarySequence Homology, Nucleic AcidModels, MolecularSequence Analysis, DNAEnteropathogenic Escherichia coliSignal TransductionColiphagesPromoter Regions, GeneticShiga-Toxigenic Escherichia coliAnti-Bacterial AgentsModels, BiologicalMutagenesis, Site-DirectedCell MembraneEnterotoxigenic Escherichia coliProtein ConformationCulture MediaTransfectionPolymerase Chain ReactionGene Expression RegulationChromosome MappingProtein BiosynthesisBacterial AdhesionHydrogen-Ion ConcentrationBacterial ToxinsFecesEnterohemorrhagic Escherichia coliSaccharomyces cerevisiaeConserved SequenceRecombination, GeneticMutagenesisUropathogenic Escherichia coliCells, CulturedPhenotypeGenetic VectorsRabbitsFimbriae, Bacterialbeta-GalactosidaseTime FactorsNucleic Acid ConformationStructure-Activity RelationshipDNA RepairProteinsEscherichia coli VaccinesMembrane Transport ProteinsDiarrheaBiological Transport
EscherichiaExpressed in mammalian cellsInsectCellsGenesGeneYeastBacterialGenomeVectorsO157SpeciesPromoterMembraneEnzymePlantHumansMouseWorkAnimalsBaculovirusSelecting mammalian cellsO157:H7BacteriaProteinsEukaryoticStrainBacteriumMetaboliteReductaseChromosomeEPECRecombinant protein expressionShigaMolecularInfectionHumanEnvironmentalVirusesCommonlyExpression systemsHostActivitySystems
Escherichia15
Expressed in mammalian cells1
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal mCFP (monomeric) tag. (origene.com)
Insect4
  • Vectors in the His- Strep pQE-TriSystem Vector Set contain promoters for E. coli , insect-, and mammalian-cell expression systems. (qiagen.com)
  • Recombinant proteins that carry two small affinity tags (the 6xHis tag and Strep -tag II) are efficiently expressed in E. coli , insect, or mammalian cells using pQE-TriSystem His- Strep vectors. (qiagen.com)
  • PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
  • The facility expresses and purifies proteins from E. coli , insect cells and mammalian cells. (embl.org)
Cells13
  • These vectors also have the capacity to direct high-yield production of protein in mammalian cells, and the option of incorporation into and expression via a T7 promoter in vaccinia virus. (washington.edu)
  • neo and gpt genes were included in some vectors to serve as selectable markers, and the dhfr gene was included in one to achieve gene amplification in mammalian cells. (washington.edu)
  • Using this method, we examined the genomic positions of 6mA in bacteria ( E. coli ), green algae ( C. reinhardtii ), and mammalian cells (HEK239T, Huh7, and HeLa cells). (nature.com)
  • Based upon an equal result in the tests of all oils from the Citrus category, the results of the available gene mutation tests in mammalian cells with a citrus oil can be read across to the other oils. (europa.eu)
  • For both orange oil and lemon oil, the gene mutation study in mammalian cells was negative. (europa.eu)
  • High efficiency chemically competent E. coli cells suitable for T7 expression with enhanced reduction of basal expression. (neb.com)
  • Enhanced BL21 derivative, chemically competent E. coli cells suitable for high efficiency transformation and protein expression. (neb.com)
  • Here, we report progress on a large-scale synthetic genomics effort to reestablish EAA biosynthetic functionality in mammalian cells. (elifesciences.org)
  • In this report, the authors devised synthetic genomic strategies to introduce essential amino-acid biosynthetic pathways into mammalian cells. (elifesciences.org)
  • While the functionalization of methionine, threonine, and isoleucine synthesis was unsuccessful, restoration of valine synthesis rendered mammalian cells partially independent of exogenous valine. (elifesciences.org)
  • For mammalian cells, we can work with most standard mammalian expression vectors (e.g. based on the pcDNA backbone) or BacMam vectors. (embl.org)
  • For transposition of the gene of interest into the baculoviral DNA, we use E. coli DH10EMBacY cells. (embl.org)
  • E. coli, P. pastoris , Mammalian cells, Plant cells and in vitro by cell free synthesis. (lu.se)
Genes1
  • With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)
Gene3
  • A T7 expression plasmid and the same plasmid containing a gene encoding a toxic mammalian protein were transformed into each host. (neb.com)
  • A T7 expression plasmid containing a gene encoding an E. coli protein was transformed into each host, grown to 0.6 OD and induced for 3 hours. (neb.com)
  • The TLR1-GFP fusion gene is cloned in the pUNO plasmid under the control of the strong and ubiquitous mammalian promoter EF1α/HTLV. (invivogen.com)
Yeast1
  • Our results with E. coli , in conjunction with recent observations made with the yeast Saccharomyces cerevisiae , raise the possibility that the SRP receptor-mediated formation of intracellular membrane networks is governed by evolutionarily conserved principles. (rupress.org)
Bacterial2
  • ara You can easily see GFP fluorescence with a standard transilluminator in colonies of E. coli expressing GFP from good bacterial promoters. (bio.net)
  • Mammalian apoptosis-inducing protein, HAP, induces bacterial cell death. (bvsalud.org)
Genome3
  • Our results imply that rare 6mA in the mammalian genome is introduced by RNA m 6 A machinery via a non-targeted mechanism. (nature.com)
  • This work demonstrates that mammalian metabolism is amenable to restoration of ancient core pathways, paving a path for genome-scale efforts to synthetically restore metabolic functions to the metazoan lineage. (elifesciences.org)
  • To facilitate mammalian genome engineering applications, we provide a web-based software tool to guide the selection and validation of target sequences as well as off-target analyses. (cdc.gov)
Vectors2
  • Vectors are described here that contain the intermediate-copy-number P15A ori for cloning, stable propagation and higher-yield production of plasmid DNA in E. coli, and the f1 ori for propagation as single-stranded phage. (washington.edu)
  • For expression in E. coli , we accept all commonly used expression vectors. (embl.org)
O1573
  • Both E. coli O157:H7 and non-O157:H7 serotypes can cause human disease and among non-O157 infections, up to 70% of human infections are attributed to six non-O157 STEC serogroups (O26, O45, O103, O111, O121, and O145) ( Bosilevac and Koohmaraie, 2012 ). (frontiersin.org)
  • In the United States, the predominant EHEC serotype associated with serious disease is E. coli O157:H7. (cdc.gov)
  • HUS complicates approximately 5% to 8% of infections caused by E. coli O157:H7. (cdc.gov)
Species2
  • The EPR and thermodynamic properties of semiquinone (SQ) species stabilized by mammalian succinate:quinone reductase (SQR) in situ in the mitochondrial membrane and in the isolated enzyme have been well documented. (lu.se)
  • E. coli QFR activity and the fast relaxing SQ species observed in the mutant enzyme are sensitive to the inhibitor 2-n-heptyl-4-hydroxyquinoline N-oxide (HQNO). (lu.se)
Promoter3
  • The SR alpha promoter, encephalomyocarditis (EMC) virus untranslated leader sequence, and poly(A) signal sequence serve as a high-yield mammalian cell expression cassette without the requirement for mRNA capping. (washington.edu)
  • 1988). SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. (invivogen.com)
  • I don't know if you can from a mammalian promoter such as CMV. (bio.net)
Membrane3
  • Previous in vivo studies indicated that unlike the E. coli signal recognition particle (SRP), the SRP receptor FtsY is required for membrane targeting of ribosomes. (rupress.org)
  • Membrane-bound ribosomes in E. coli were extensively studied over 20 years ago. (rupress.org)
  • Recently, it has been recognized that membrane-bound ribosomes are crucial for biogenesis of integral membrane proteins in E. coli , thus renewing interest in ribosome targeting to and association with the membrane in this organism. (rupress.org)
Enzyme1
  • Expression and regulation of the mammalian SUMO-1 E1 enzyme. (nih.gov)
Plant2
  • EC 1.13.11.27) from the plant Arabidopsis thaliana with and without an herbicidal benzoylpyrazole inhibitor that potently inhibits both plant and mammalian HPPDs. (rcsb.org)
  • Detailed comparisons of the plant and mammalian HPPD-ligand structures suggest a structural basis for the high degree of plant selectivity of certain HPPD inhibitors and point to design strategies to obtain potent and selective inhibitors of plant HPPD as agrochemical leads. (rcsb.org)
Humans1
  • Any mammalian metabolite produced during a metabolic reaction in humans ( Homo sapiens ). (ebi.ac.uk)
Mouse1
  • Any mammalian metabolite produced during a metabolic reaction in a mouse ( Mus musculus ). (ebi.ac.uk)
Work1
  • Altogether, this work was found to be of substantial interest as it provides pioneering evidence that mammalian systems may be permissive to the restoration of essential amino acid biosynthetic pathways and is thus anticipated to have a broad impact in the fields of synthetic biology, biotechnology and beyond. (elifesciences.org)
Animals1
  • Colonization by EHEC is related to the pathogen's ability to form attaching and effacing lesions (intestinal mucosal changes seen in transmission electron microscopy and originally seen in intestines of animals infected with enteropathogenic E. coli [EPEC]) ( 1 ). (cdc.gov)
Baculovirus2
  • Among the available expression systems, the baculovirus expression vector system (BEVS) has many advantages for the expression of these proteins, including 1) high level expression by strong promoters (Polyhedrin and P10), 2) post-translational modifications similar to those generated by mammalian cell expression, and 3) more reasonable cost than mammalian cell culture. (omicsonline.org)
  • Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
Selecting mammalian cells1
  • Burbelo said this stems from selecting mammalian cells and its biological machinery as the recombinant cloning vector to make the antigens. (nih.gov)
O157:H73
  • Containers of SoyNut Butter from lots #243162 and 244161 tested positive for E. coli O157:H7. (marlerblog.com)
  • Whole genome sequencing revealed that the same strain of E. coli O157:H7 was found in clinical isolates from sick people and containers of I.M. Healthy SoyNut Butter. (marlerblog.com)
  • [5] The testing done to distinguish E. coli O157:H7 from its other E. coli counterparts is called serotyping. (marlerblog.com)
Bacteria5
  • Enteropathogenic E. coli (EPEC) belongs to a group of bacterial pathogens that induce epithelial cell actin rearrangements resulting in pedestal formation beneath adherent bacteria. (nih.gov)
  • Most rod-shaped bacteria, including E. coli and B. subtilis , grow by inserting new cell-wall material into their lateral sidewalls 8 . (nature.com)
  • A new engineered strain of E. coli bacteria non-destructively detected and recorded an environmental signal in the mouse gut, and remembered what it 'saw. (sciencedaily.com)
  • But now a new engineered strain of E. coli bacteria could deliver status updates from this complex landscape to help keep gastrointestinal diseases at bay. (sciencedaily.com)
  • In laboratory experiments, the switch turned on within a few hours of exposure to the antibiotic- and stayed in this 'ON' state inside E. coli for a week or more, even as the bacteria grew and divided. (sciencedaily.com)
Proteins2
  • Here, we report the generation and validation of nAbs against a set of proteins prominently expressed at specific subcellular sites in mammalian brain neurons. (nih.gov)
  • BPS Bioscience Inc. specializes in mammalian expression system to produce recombinant proteins. (biosciregister.com)
Eukaryotic2
  • These toxins are released into the extracellular milieu, but they act upon targets within the eukaryotic (mammalian) cytosol. (usda.gov)
  • The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
Strain1
  • Epidemiologic investigation determined that 32 people ill with this strain of E. coli had been infected by eating or attending a facility that served I.M. Healthy SoyNut Butter. (marlerblog.com)
Bacterium2
  • [4] The E. coli bacterium is among the most extensively studied microorganism. (marlerblog.com)
  • The genetic switch the team inserted in E. coli came from lambda phage, a virus that commonly attacks this bacterium. (sciencedaily.com)
Metabolite2
  • Any mammalian metabolite produced during a metabolic reaction in a mouse ( Mus musculus ). (ebi.ac.uk)
  • Any mammalian metabolite produced during a metabolic reaction in humans ( Homo sapiens ). (ebi.ac.uk)
Reductase1
  • 14. Interactions of mammalian cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b(5) enzymes. (nih.gov)
Chromosome1
  • For example, E. coli and B. subtilis exhibit multifork chromosome replication during rapid growth under nutrient-rich conditions, whereas C. crescentus does not reinitiate chromosome replication until the previous round of replication as well as cell division have been completed 1 , 2 . (nature.com)
EPEC1
  • EPEC shares many genetic and phenotypic similarities with enterohemorrhagic E. coli (EHEC), a zoonotic pathogen that infects children and adults, with a major reservoir being livestock ( Hartland and Leong, 2013 ). (frontiersin.org)
Recombinant protein expression1
  • We developed a novel knockout strategy to enhance recombinant protein expression in E. coli based on the phenomenon that recombinant protein expression triggers stress response associated with downregulation of key metabolic pathways. (nih.gov)
Shiga2
  • A stool specimen collected on March 6 at Pediatric Associates of the Northwest tested culture positive at Oregon State Public Health Laboratory for shiga toxin-producing E. coli O157 (Case ID#557072). (marlerblog.com)
  • A stool specimen collected on March 14 at Legacy Emmanuel Medical Center tested culture positive at Oregon State Public Health Laboratory for shiga toxin-producing E. coli O157 (Case ID#556818). (marlerblog.com)
Molecular1
  • degree in Biotechnology, Biochemistry, Cell Biology or a related field - research experience in molecular biology (molecular cloning), E. coli culturing and mammalian cell culture - capacity to think analytically and creatively - high motivation - ability to work in an international team - an integrative and cooperative personality with excellent communication and social skills - fluency in English - written and oral. (noorderlink.nl)
Infection2
  • The causal link between Julian Murph's E. coli O157 infection and I.M. Healthy brand SoyNut Butter is clear. (marlerblog.com)
  • Finley did not ingest SoyNut Butter but acquired his E. coli infection through secondary transmission from Julian Murph. (marlerblog.com)
Human5
  • Another important difference between the bacterial and mammalian ATs is that the bacterial protein was also able to remove methyl groups from the O4-position of thymine in methylated DNA or poly(dT) but the AT from rat liver or human fibroblasts did not repair O4-methylthymidine. (nih.gov)
  • Recombinant Human BRD1 corresponding to amino acids 556-688 that contains the bromodomain sequences of BRD1 (accession number NP_055392.1) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag. (creativebiomart.net)
  • Recombinant Human IL-2 (Mammalian-expressed) Protein ( 10453-IL ) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. (rndsystems.com)
  • Mammalian-expressed Recombinant Human IL-2 has similar activity to E.coli -derived Recombinant Human IL-2 ( 202-IL ). (rndsystems.com)
  • 2 μg/lane of Recombinant Human IL-2 (Mammalian-expressed) Protein ( 10453-IL ) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa. (rndsystems.com)
Environmental1
  • The key to turning E. coli into gut reporters was to insert a well-known genetic switch that flips when it senses a specific environmental cue. (sciencedaily.com)
Viruses1
  • Mammalian Pathogenicity and Transmissibility of H1 Variant Influenza Viruses from the Swine Reservoir. (cdc.gov)
Commonly1
  • HUS is a debilitating condition caused by E. coli that is commonly characterized by kidney failure, but may also lead to brain damage, seizures, and diabetes. (marlerblog.com)
Expression systems1
  • But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. (antikoerper-online.de)
Host1
  • Thus, this bacterial pathogen inserts its own receptor into mammalian cell surfaces, to which it then adheres to trigger additional host signaling events and actin nucleation. (nih.gov)
Activity1
  • In E. coli such exposure increased the activity more than 100-fold. (nih.gov)
Systems1
  • Recently, some improved E. coli systems with phosphorylation or disulfide-bond ability have been developed [ 2 ], but they are not always successful. (omicsonline.org)