The following product was used in this experiment: ZO-1 Monoclonal Antibody (ZO1-1A12) from Thermo Fisher Scientific, catalog # 33-9100, RRID AB_2533147. (thermofisher.com)
Western blot was performed using Anti-ZO-1 Monoclonal Antibody (ZO1-1A12) (Product # 33-9100) and a 225 kDa band corresponding to ZO-1 was observed across along with uncharacterized band. (thermofisher.com)
This enables the rational design of the alpha helical coiled-coil motif. (springeropen.com)
Extracellular matrix2
Among the extracellular matrix compounds used in this study, two (collagen I and fibronectin) allowed only a weak attachment of cortical neurons to the substratum, while the others (collagen IV, laminin, and basal lamina from Engelbreth-Holm-Swarm sarcoma) allowed both firm attachment and moderate to extensive neurite outgrowth from neuronal cell bodies. (curehunter.com)
Mouse anti-β-actin (Sigma) or rabbit anti-GAPDH (Trevigen MD USA) antibody was utilized as an example launching control. (biobender.com)
Minneapolis MN) or mouse anti-ubiquitin antibody (Santa Cruz Biotechnology Santa Cruz CA). The HRP-conjugated supplementary antibodies had been rabbit anti-goat IgG antibody (DAKO Copenhagen Denmark) and goat anti-mouse Dinaciclib (SCH 727965) light string antibody (Millipore). (hiv-proteases.com)
Proteins4
Moreover, integrins, laminins, NF-κB and other regulative molecules were found as crucial proteins. (nature.com)
Using a broad spectrum anti-acetyl antibody, the researchers determined that one of the acetylated proteins is myosin. (cytoskeleton.com)
Here, we report the development of a temperature-responsive fusion protein (ELP-DCN) comprised of an elastin-like polypeptide (ELP), poly-aspartic acid (D), antibody-binding domain C (C), and NanoLuc luciferase (N). ELP-DCN proteins form nanoparticles above a certain threshold temperature that display an antibody-binding domain and NanoLuc luciferase on their surface. (bvsalud.org)
The proteins had been used in a polyvinylidene difluoride (PVDF) membrane (Millipore MA USA) and incubated with properly diluted specific major antibodies. (biobender.com)
Detection6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Nuclear Factor Kappa B (NFkB) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (resourcerecovery.be)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Nuclear Factor Kappa B (NFkB) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (resourcerecovery.be)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Visfatin (VF) in serum, plasma and other biological fluids. (2sars.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Visfatin (VF) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. (2sars.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Integrin Beta 6 (ITGb6) in Tissue homogenates, cell lysates and other biological fluids. (antibody-tech.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) in Tissue homogenates, cell lysates and other biological fluids. (chemcarbindia.com)
Molecules1
ELP-DCN nanoparticles can be applied for enhancement of immunoassay systems because they provide more antibody-binding sites and an increased number of luciferase molecules, resulting in an increase in assay signal. (bvsalud.org)
Structure2
The coiled-coil structure of the long arm is formed by three chains (α3, β3 and γ2) covalently linked via interchain disulfide bonds ( 8 ). (spandidos-publications.com)
We have used monoclonal antibodies (mAbs) as probes to study the dependence of the structure of different regions of the alphaM subunit on association with the beta2 subunit in the alphaM/beta2 heterodimer. (embl.de)
Region3
Modified lysine residues occurred in the actin binding region as well as the coiled-coil tail and the hinge regions of myosin (see Fig. 1). (cytoskeleton.com)
Note that acetylation covers all of the important functional areas of myosin from actin and ATP binding to the hinge region and even the coiled-coil tail. (cytoskeleton.com)
A single monomer of CD23 comprises a C\terminal CTLD globular region connected to a single hydrophobic membrane\spanning region by an \helical coiled\coil stalk, followed by a short N\terminal cytoplasmic domain name [21, 22]. (treatmentforprostatecancer.info)
Monoclonal antibodies2
We have used monoclonal antibodies (mAbs) as probes to study the dependence of the structure of different regions of the alphaM subunit on association with the beta2 subunit in the alphaM/beta2 heterodimer. (embl.de)
The introduction of monoclonal antibodies in 1974 brought to mind Paul Ehrlich's turn-of-the-century insight that molecules could serve as "magic bullets" that home to pathological organisms with precision (http://nobel.sdsc.edu/medicine/laureates/1908/ehrlich-bio.html). (biobender.com)
Double Antibody10
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Guanylate Cyclase Activator 2A (GUCA2A) in serum, plasma, tissue homogenates and other biological fluids. (resourcerecovery.be)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Guanylate Cyclase Activator 2A (GUCA2A) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (resourcerecovery.be)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Dihydropyrimidinase Like Protein 2 (DPYSL2) in tissue homogenates, cell lysates and other biological fluids. (antibody-tech.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Dihydropyrimidinase Like Protein 2 (DPYSL2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (antibody-tech.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Cystatin A (CSTA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (resourcerecovery.be)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Internexin Neuronal Intermediate Filament Protein Alpha (INa) in Tissue homogenates, cell lysates and other biological fluids. (jemsec.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Internexin Neuronal Intermediate Filament Protein Alpha (INa) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (jemsec.com)
This ELISA kit is based on the principle of double-antibody sandwich technique.Be used only for research purposes, not be used for medical diagnosis.In order to prevent plagiarism, the site hides some of the product manual, if you want to access detailed product specifications, please write in to request. (cdbelisa.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Death Associated Protein Kinase 1 (DAPK1) in Tissue homogenates, cell lysates and other biological fluids. (kits-elisa.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Death Associated Protein Kinase 1 (DAPK1) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
ELISA2
Description: A sandwich ELISA for quantitative measurement of Rat Cathepsin Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (youngresearch.eu)
The ELISA process to detect anti-GFP antibodies was performed using a previously explained method (Sharma et al. (biobender.com)
Extracellular2
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. (nih.gov)
2011 and it has been well-characterized that myelinating Schwann cells secrete a myriad of trophic and adhesive factors including neurotrophins cytokines and lamininextracellular matrix proteins as they execute the regeneration system [Stoll and Muller 1999 Jessen and Mirsky 2005 Campana 2007 Studies have recognized the part of reciprocal signaling between tumor cells and the nerve environment or stromal cells as promoters of perineural invasion [Dai et al. (hiv-proteases.com)
Proteins2
[ 7 ] Tropomyosins are a family of actin-binding coiled-coil proteins that help to regulate calcium-dependent muscle contraction. (medscape.com)
In this study, to characterize their functions comparatively, we produced recombinant proteins of LG45 modules of human laminin α1-5 chains. (bvsalud.org)
Basement membrane2
Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. (nih.gov)
Laminins are a family of heterotrimers composed of α-, ß-, and γ-chains in the basement membrane. (bvsalud.org)
Protein3
The protein encoded by this gene belongs to the laminin family of secreted molecules. (nih.gov)
Light meromyosin consists of coiledcoil protein structure. (cytoskeleton.com)
Like antibodies aptamers most frequently function through high-affinity binding to a target protein. (biobender.com)
Chains2
Heavy meromyosin consists of the myosin head subfragment-1 domain (S1), its associated light chains (essential light chains and regulatory light chains), and the coiled-coil subfragment -2 domain. (cytoskeleton.com)
Five α chains contain laminin globular (LG) domain consisting of five tandem modules (LG1-5 modules) at their C-terminus. (bvsalud.org)
Domain1
Moreover, α5LG45 modules bound to the E8 fragment, which includes the C-terminus of the laminin coiled-coil (LCC) domain and LG1-3 modules, but α2LG45 modules did not. (bvsalud.org)
Sensitivity2
There was reduced affinity for F-actin, reduced formation of preferred α/β heterodimers in favor of α/α heterodimers, destabilization of the coiled coil, impaired binding to tropomodulin, and reduced sensitivity of isometric force production to activating calcium. (medscape.com)
invasion was dependent on ITGA6p formation and ITGB1 as determined by function-blocking antibodies. (hiv-proteases.com)
Blocking ITGA6p formation using the J8H antibody or ITGB1 function using the AIIB2 antibody (Fig. 3A B C) eliminated or significantly decreased S16 induced migration of DU145 Personal computer3 and CFPAC1 cells ( (hiv-proteases.com)
High2
Indeed the high affinity and specificity of antibodies provide some of the key properties in Ehrlich's concept. (biobender.com)
Having high affinity and specificity and being synthetic polymers aptamers combine the advantages of antibodies and small peptides in tissue targeting. (biobender.com)
Blood2
Despite some successes in tissue targeting antibodies are saddled with a fundamental disadvantage: their large size (～155 kDa) results in BTLA slow tissue penetration and long blood residence. (biobender.com)
To decrease blood half-life while maintaining target specificity a second generation of smaller antibody fragments has been designed (10 11 Antibody pretargeting strategies also show promise (12-14) and small peptides can have excellent pharmacokinetic profiles (15). (biobender.com)
Test1
The reciprocal of highest test serum dilution with absorbance above control (mean± 2 standard deviation) was judged as the antibody titer. (biobender.com)