Cofilin binds actin. Medical search. Frequent questions

Cofilin can be an actin-binding protein that depolymerizes and/or TRV130 HCl (Oliceridine) severs actin filaments. (ap26113.com)
Growth-cone collapse induced by ephrin-As, a family of repulsive axon guidance molecules, is impaired upon R62D expression, resulting in perseverance of ring-shaped F-actin filaments. (jneurosci.org)
In living cells, cofilin is not necessary for actin assembly on endocytic membranes but is recruited to molecularly aged adenosine diphosphate actin filaments and is necessary for their rapid disassembly. (rupress.org)
Cofilin binds preferentially to ADP-actin subunits within actin filaments. (rupress.org)
This binding induces a twist in the filament, accelerates the release of Pi from ADP-Pi subunits, and severs actin filaments ( Pollard and Borisy, 2003 ). (rupress.org)
The molecular determinants of cofilin recruitment to actin filaments in vivo have not been fully investigated. (rupress.org)
This localization was found to be unaffected by manipulations that were expected to change polymerization rates at the leading edge, which should alter the relative abundance of ATP-, ADP-Pi-, and ADP-actin subunits within the filaments. (rupress.org)
The cytoskeleton is typically divided into three distinct components: microtubules, intermediate filaments and actin[ 4 , 5 ]. (biorxiv.org)
Unlike the globular units of microtubules and actin filaments, intermediate filaments are themselves constructed from filamentous sub-units, and confer strength as well as stress resistance to the cell[ 10 ]. (biorxiv.org)
Finally, actin filaments, also known as microfilaments, are the narrowest components of the cytoskeleton and are constructed from globular actin sub-units (G-actin) that assemble to form a helical structure 5-7nm in diameter[ 12 ]. (biorxiv.org)
Actin microfilaments are present as both individual filaments and bundled into thicker filaments, and play a key role in plant cell growth and internal transport[ 14 ]. (biorxiv.org)
By focusing on the stabilizing protein tropomyosin Cdc8, bundling protein fimbrin Fim1, and severing protein coffin Adf1, we examined how their pairwise and collective interactions with actin filaments regulate their activity and segregation to functionally diverse F-actin networks. (elifesciences.org)
Utilizing multi-color TIRF microscopy of in vitro reconstituted F-actin networks, we observed and characterized two distinct Cdc8 cables loading and spreading cooperatively on individual actin filaments. (elifesciences.org)
Furthermore, Cdc8, Fim1, and Adf1 all compete for association with F-actin by different mechanisms, and their cooperative association with actin filaments affects their ability to compete. (elifesciences.org)
Dephosphorylation and activation of cofilin lead to severing and depolymerization of existing actin filaments. (uni-muenchen.de)
it is initiated by membrane protrusion, which is mediated by the dynamics of actin filaments [ 1 - 2 ] that assemble as a network at the leading edge of migrating cells [ 3 ]. (oncotarget.com)
Cofilin stimulates actin depolymerization and severance of actin filaments at the protruding ends, thereby promoting their rapid turnover and determining the direction of cell movement [ 4 - 5 ]. (oncotarget.com)
How do actin filaments form higher-order assemblies that produce and respond to force? (mechanobio.info)
Cells exert traction forces on the ECM and generate tension at focal adhesions through actin stress fibers , which are higher-order structures in the cytoplasm that consist of parallel contractile bundles of actin and myosin filaments. (mechanobio.info)
Cell tension is generated along the actin filaments by the movement of myosin II motor proteins along the filaments (see contractile bundles). (mechanobio.info)
It binds to actin in a one-to-one molar ratio, and stimulates both the severing of actin filaments and depolymerization of actin subunits from the actin filament end. (reactome.org)
Filopodia are finger-like projections supported by a cytoskeleton of bundled actin filaments ( Gallo and Letourneau, 2000 ). (jneurosci.org)

Regulates actin cytoskeleton dynamics. (thermofisher.com)
Lamellipodial protrusion and directional migration of carcinoma cells towards chemoattractants, such as epidermal growth factor (EGF), depend upon the spatial and temporal regulation of actin cytoskeleton by actin-binding proteins (ABPs). (rupress.org)
The actin cytoskeleton is essential in eukaryotes, not least in the plant kingdom where it plays key roles in cell expansion, cell division, environmental responses and pathogen defence. (biorxiv.org)
In addition to these results, our algorithm is designed to be easily adaptable to other tissues, mutants and plants, and so will be a valuable asset for the study and future biological engineering of the actin cytoskeleton in globally-important crops. (biorxiv.org)
In addition, this problem is by far the most acute for actin, the narrowest element of the cytoskeleton, which is ubiquitous throughout eukaryotic cells. (biorxiv.org)
The fission yeast actin cytoskeleton is an ideal, simplified system to investigate fundamental mechanisms behind cellular self-organization. (elifesciences.org)
The morphological and functional changes of platelets require a drastic remodeling of the actin cytoskeleton regulated by numerous actin-binding proteins and signaling molecules such as the family of Rho-GTPases. (uni-muenchen.de)
The actin cytoskeleton is a complex structure that performs a wide range of cellular functions. (edu.au)
In particular, 1) the structure of actin was resolved from crystals in the absence of cocrystallized actin binding proteins (ABPs), 2) the prokaryotic ancestral gene of actin was crystallized and its function as a bacterial cytoskeleton was revealed, and 3) the structure of the Arp2/3 complex was described for the first time. (edu.au)
The Rho family of GTPases regulate the actin cytoskeleton. (jneurosci.org)

Coronin, actin binding protein, 1B also known as CORO1B is a protein which in humans is encoded by the CORO1B gene. (wikipedia.org)
Studies related to this protein are as follows: Coronin 1B antagonizes cortactin and remodels Arp2/3-containing actin branches in lamellipodia. (wikipedia.org)
Rho-kinase regulates a second pathway that involves activation of LIM-kinases (LIMKs) and subsequent phosphorylation and inactivation of cofilin, an actin dynamizing protein. (uni-muenchen.de)
Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. (thermofisher.com)
Mikula KM, Kolodziejczyk R, Goldman A. Structure of the UspA1 protein fragment from Moraxella catarrhalis responsible for C3d binding. (helsinki.fi)
The cryo-EM analysis also revealed a population of free V 1 bound to Oxidation Resistance 1 (Oxr1p), a poorly characterized protein that has been implicated in protecting cells from oxidative stress. (upstate.edu)
For control lysate, the recombinant Cofilin 1 protein was exposed to buffer only (Cat. (ecmbio.com)
Cofilin activity is regulated by its phosphorylation status, and is negatively regulated by serine/threonine kinases, LIM kinases, and testis-specific protein kinase via phosphorylation at Ser-3 [ 6 - 8 ]. (oncotarget.com)
Phospho-cofilin is dephosphorylated and reactivated by Slingshot (SSH), a member of the conserved family of protein phosphatases [ 9 - 10 ]. (oncotarget.com)
Each myosin motor protein possesses ATPase activity and functions in a cyclical manner that couples ATP binding and hydrolysis to a conformational change in the protein. (mechanobio.info)
Loss of the lysosomal membrane-tethering homotypic fusion and protein sorting (HOPS) complex disrupts this actin clearing and ciliogenesis, but it remains unclear how the ciliary function of HOPS relates to its canonical function in regulating late endosome-lysosome fusion. (bvsalud.org)
To elucidate the mechanism of actin disassembly, we characterized molecular kinetics of the major filament end-binding proteins Arp2/3 complex and capping protein (CP) using single-molecule speckle microscopy. (silverchair.com)
Atomistic molecular dynamics simulation (500 ns) revealing how actin-binding protein cofilin associates with the plasma membrane. (utu.fi)
2009). CFL1 is a member of the ADF (actin-depolymerizing factor) protein family that is involved in regulating actin dynamics in the growth cone. (reactome.org)
Memo, a 297-amino-acid protein, has homology to class III nonheme iron-dependent dioxygenases, however it has not been shown to display metal binding or enzymatic activity. (ecmbio.com)

In contrast in a reducing environment even phosphatidylinositol 4 5 -bound cofilin becomes active leading to actin dynamics in the vicinity of the plasma membrane. (ap26113.com)
The dual function of cofilin namely depolymerization and severing makes it a key molecule controlling actin dynamics. (ap26113.com)
Membrane-bound dephosphorylated cofilin could be triggered by PLC-dependent PI(4 5 cleavage releasing dephosphorylated cofilin into the cytoplasm 38 40 Thereby the cytoplasmic pool of activated cofilin is increased and actin dynamics are reinforced. (ap26113.com)
5 Actin dynamics critically depend on members of the ADF/cofilin family that comprises muscle cofilin (m-cofilin), non-muscle cofilin (n-cofilin), and ADF (actin depolymerizing factor). (nature.com)
In addition to regulating cytoskeletal dynamics in the cytoplasm, actin modulates nuclear gene expression. (jneurosci.org)
Moreover, R62D NLS decreases neuronal motility similar to the cytoplasmic R62D actin mutant although R62D NLS has no access to cytoplasmic actin dynamics. (jneurosci.org)
Thus, herein we provide first evidence that neuronal motility not only depends on cytoplasmic actin dynamics but also on the availability of actin to modulate nuclear functions such as gene transcription. (jneurosci.org)
In addition to regulation of cytoplasmic cytoskeletal dynamics, a little appreciated property of actin signaling is modulation of gene expression so far only reported for non-neuronal cells. (jneurosci.org)
In this study, we analyzed the dynamics of fluorescently tagged cofilin and the role of cofilin-mediated actin turnover during endocytosis in Saccharomyces cerevisiae . (rupress.org)
However, the role of actin filament disassembly (a key step in actin dynamics) during actin-mediated endocytosis is less clear. (rupress.org)
These small highly conserved actin-binding proteins are essential regulators of actin dynamics in living cells. (rupress.org)
Cofilin and its role in actin dynamics have been studied extensively in vitro. (rupress.org)
The signaling pathway Rho-kinase/LIMKs/cofilin phosphorylation during platelet activation and the question, how the phosphorylation of cofilin affects the actin dynamics underlying platelet activation, has not previously been studied. (uni-muenchen.de)
ADF)/cofilin family which regulates actin dynamics. (tech-strategy.org)
Alteration of G/F-actin ratio and actin dynamics in response to AITC G/F-actin ratio is an indicator of the extent of actin dynamics and might be responsible for regulating apoptosis [5]. (tech-strategy.org)
Fmoc-Lys(Me3)-OH chloride To understand the mechanism of AITC-mediated apoptosis through affecting actin dynamics we separated actin into G and F fractions and evaluated their relative content. (tech-strategy.org)
CP dissociates slower from actin stress fibers than from the lamellipodial actin network, suggesting that CP dissociation correlates with actin filament dynamics. (silverchair.com)
Our data demonstrate that neurotrophin binding to p75 NTR is necessary and sufficient to regulate filopodial dynamics. (jneurosci.org)
This effector may be important for mediating ErbB2-regulated changes in actin and MT dynamics during cell motility. (ecmbio.com)
In addition, Memo may regulate actin dynamics by promoting cofilin depolymerizing and severing of F-actin. (ecmbio.com)

These observations led to the hypothesis that cofilin localization is largely dependent on accessory factors or other regulatory inputs, such as phosphorylation or pH. (rupress.org)
Surprisingly, the activation of LIMK-1 failed to increase cofilin phosphorylation during shape change induced by LPA as well as by thrombin. (uni-muenchen.de)
Our results showing that only dephosphorylated (activated) cofilin binds with F-actin support previous observations that the state of cofilin phosphorylation determines its association with F-actin. (uni-muenchen.de)
LIMK1 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. (thermofisher.com)
Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. (ecmbio.com)
This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. (ecmbio.com)
The EPHB2-FAK pathway partially promotes dendritic spine stability through LIMK-mediated cofilin (CFL1) phosphorylation (Shi et al. (reactome.org)
After phosphorylation, CFL1 is inactive, loses its affinity for actin and dissociates from G-actin monomers. (reactome.org)

In 1994 we demonstrated for the TRV130 HCl (Oliceridine) very first time that cofilin translocation in to the nucleus succeeds triggering of the cell surface area receptor namely Compact disc2 excitement of untransformed human being T cells 37. (ap26113.com)
of cofilin on serine 3 must enable its nuclear translocation 35. (ap26113.com)
Cofilin consists of a nuclear localization series (KKRKK) like the nuclear translocation sign series of simian disease 40T antigen 68-69 (or in petri meals covered with integrin-ligands data and data produced from T cells or additional cellular systems. (ap26113.com)
Recent studies reported a mitochondrial translocation of n-cofilin during apoptosis. (nature.com)
8 Recently, it was shown that n-cofilin translocates to mitochondria during the early phase of apoptosis, but the mechanism for this translocation remained obscure. (nature.com)
V-ATPase is made of two subcomplexes: a cytosolic V 1 that carries out ATP hydrolysis, and a membrane bound V o that is responsible for proton translocation. (upstate.edu)
Mechanistic study revealed that interruption of ROCK1/PTEN/PI3K signaling pathway plays a critical role in AITC-mediated dephosphorylation and mitochondrial translocation of cofilin and apoptosis. (tech-strategy.org)

A major modulator of actin filament disassembly is the actin-depolymerization factor/cofilin family of proteins (hereafter referred to as cofilin). (rupress.org)
Severing increases the number of actin filament pointed ends and, in conjunction with capping of barbed ends by capping proteins, stimulates filament disassembly ( Andrianantoandro and Pollard, 2006 ). (rupress.org)
Genetic and biochemical analysis of a conditional allele of cofilin showed that the actin filament disassembly activity of cofilin is essential for fluid-phase endocytosis ( Lappalainen and Drubin, 1997 ). (rupress.org)
Biochemical experiments showed that Oxr1p binds and promotes V-ATPase disassembly. (upstate.edu)

Activated LIMK phosphorylates CFL1 on the conserved serine 3 residue located near the actin-binding site. (reactome.org)

In combination with myosin motors, actin aids in transport by providing the roads and pathways for cellular cargo[ 13 ]. (biorxiv.org)

Furthermore, organization of the lamellipodial actin network is coordinated by signaling from Memo to the RhoA-mDia1 pathway localized to the plasma membrane. (ecmbio.com)

Upon release, we find that cofilin binds to and severs F-actin, which is coincident with actin polymerization and lamellipod formation. (rupress.org)

however, this is mainly based on in vitro-binding and structural studies, and generally in vivo evidence is lacking. (rupress.org)
For preparation of Ser-3 phosphorylated Cofilin, purified human Cofilin 1 was phosphorylated in vitro with His-tagged mouse recombinant LIM Kinase 1 for 30 min. (ecmbio.com)

To elucidate the general importance of ADF/cofilin activity for apoptosis, we exposed mouse embryonic fibroblasts deficient for n-cofilin, ADF (actin depolymerizing factor), or all ADF/cofilin isoforms to well-characterized apoptosis inducers. (nature.com)
SRF controls gene transcription of various actin isoforms (e.g. (jneurosci.org)
Here we analyzed neuronal actin signaling by a novel approach using actin mutant isoforms known to favor or preclude actin polymerization. (jneurosci.org)
Filamin binds all actin isoforms (e.g. (mechanobio.info)

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell migration, cytokinesis, axon development, and immune cell activation. (ecmbio.com)

Both extrinsic factors of the microenvironment and intrinsic signal transduction events mediate this cofilin orchestration through phospho- phospholipid and redox regulation of cofilin within human T cells (look at from the bull's-eye formed organization from the SMACs in the T-cell membrane. (ap26113.com)
Here, we provide the first in vivo data that directly visualize the spatial and temporal regulation of cofilin by PIP 2 in living cells. (rupress.org)

The consequences of decreasing actin flux are manifested by decreased but not blocked endocytic internalization at the plasma membrane and defects in late steps of membrane trafficking to the vacuole. (rupress.org)

transmission 1) together with accessory receptors (transmission 2) or triggering through the chemokine SDF1α (stromal cell-derived element 1α) induce Ras-dependent dephosphorylation of cofilin which is important for immune synapse formation T-cell activation and T-cell migration. (ap26113.com)
Inhibition of the Rho-kinase/LIMK-1 pathway unmasked cofilin dephosphorylation suggesting that during shape change the simultaneous activation of a cofilin phosphatase counteracts the effect of LIMK-1 for phosphorylating cofilin. (uni-muenchen.de)
Furthermore, the results clearly showed that cofilin dephosphorylation is also independent and upstream of secretion, since the onset of cofilin dephosphorylation was as rapid as secretion in thrombin-stimulated platelets and also occurred in the absence of dense granule secretion in LPA (10 µM)-stimulated platelets. (uni-muenchen.de)
Since the kinetics of cofilin phospho-cycle was similar during secretion and platelet aggregation in LPA- and thrombin-stimulated cells, I propose a general two-step regulatory process for cofilin phospho-cycle underlying primarily secretion, and subsequently platelet aggregation: dephosphorylation by a cofilin phosphatase and then rephosphorylation by the Rho-kinase/LIMK-1 pathway. (uni-muenchen.de)
Our study also showed that AITC-mediated inhibition of tumor growth of mouse leukemia xenograft model is usually in association with dephosphorylation of cofilin. (tech-strategy.org)
Conclusions These findings support a model in which induction of apoptosis by AITC stems primarily from activation of ROCK1 and PTEN and inactivation of PI3K leading in turn to activation of PP1 and PP2A resulting in dephosphorylation of cofilin which binds to G-actin and translocates Fmoc-Lys(Me3)-OH chloride to mitochondria culminating in the dysfunction of mitochondria release of cytochrome c and apoptosis. (tech-strategy.org)
and in several tumor xenograft models results indicate that dephosphorylation of cofilin may contribute to AITC-mediated inhibitory effects on tumor growth of U937 Fmoc-Lys(Me3)-OH chloride xenograft mouse model. (tech-strategy.org)

The membrane-bound small fraction of cofilin can be dephosphorylated but held inactive by binding to PI(4 5 Both cytoplasmic fraction with least a percentage from the membrane-bound cofilin are turned on by T-cell costimulation. (ap26113.com)
We show that EGF induces a rapid loss of PIP 2 through PLC activity, resulting in a release and activation of a membrane-bound pool of cofilin. (rupress.org)

Cofilin knockout mice exhibit an embryonic lethal phenotype 42 and cofilin null mutants are also lethal in yeast 43. (ap26113.com)
We show that neuronal motility evoked by these actin mutants requires SRF activity. (jneurosci.org)
The actin mutants G15S and S14C favor F-actin assembly, yet differ with regard to their ABP interaction. (jneurosci.org)
Employing these actin mutants, we uncovered a function for actin signaling in growth-cone motility modulated by ephrin-A guidance cues. (jneurosci.org)
Moreover these actin mutants affect neurite elongation, an actin function which by pharmacological actin interference was ambiguous. (jneurosci.org)
Using this algorithm, we then studied a number of cases in Arabidopsis thaliana , including several different tissues, a variety of actin-affected mutants, and cells responding to powdery mildew. (biorxiv.org)

Many of these are likely to help us understand and distinguish between the structural models of actin microfilaments. (edu.au)

The following product was used in this experiment: Cofilin Polyclonal Antibody from Thermo Fisher Scientific, catalog # 10960-1-AP. (thermofisher.com)

Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility. (wikipedia.org)

Ciliogenesis in polarized epithelial cells requires Rab19-mediated clearing of apical cortical actin to allow the cilium to grow from the apically docked basal body into the extracellular space. (bvsalud.org)
2002 ) demonstrated that NT-3 binding to p75 NTR in cortical subplate neurons increases neurite length and filopodial formation. (jneurosci.org)

Actin forms the dendritic nucleation network and undergoes rapid polymerization-depolymerization cycles in lamellipodia. (silverchair.com)

Cofilin localizes towards the … TRV130 HCl (Oliceridine) Nuclear features of cofilin Furthermore to its function in the cytoplasm dephosphorylated cofilin has the capacity to translocate in to the nucleus. (ap26113.com)

Interestingly, mitochondrial association of ADF and n-cofilin during apoptosis was preceded by, and dependent on, actin that translocated by a yet unknown mechanism to mitochondria during cell death. (nature.com)
However, the molecular mechanism of cofilin recruitment to actin networks during dynamic actin-mediated processes in living cells and cofilin's precise in vivo functions have not been determined. (rupress.org)
A recent study from the Wilkens lab in collaboration with Seoul National University uncovered a novel mechanism of yeast V-ATPase regulation based on biochemical experiments and cryo electron microscopy (cryo-EM) structures of holo V-ATPase and Oxr1 bound V 1 subcomplex. (upstate.edu)

1999 ) provided evidence that p75 NTR can regulate axon extension by signaling through the RhoA pathway, such that the unbound state of p75 NTR activates RhoA, whereas neurotrophin binding to p75 NTR terminates this mode of RhoA activation. (jneurosci.org)

Such cytoskeletal changes appeared to render the eggs more receptive to sperm, as judged by the significant alleviation of polyspermy by latrunculin-A and mycalolide-B. In addition, our fluorimetric assay provided the first evidence that nicotine directly accelerates polymerization kinetics of G-actin and attenuates depolymerization of preassembled F-actin. (mdpi.com)
After stimulation with LPA and thrombin under conditions, where platelet aggregation could not occur, the kinetics of cofilin de- and rephosphorylation were unperturbed indicating their independence of integrin IIbβ3 engagement. (uni-muenchen.de)

Only dephosphorylated cofilin binds to G-actin and translocates to mitochondria during AITC-mediated apoptosis. (tech-strategy.org)

actin nucleation 30. (ap26113.com)

F-actin binding is essential for coronin 1B function in vivo. (wikipedia.org)
Cofilin is the major mediator of actin filament turnover in vivo. (rupress.org)
Defects in cofilin function alter the morphology of actin networks in vivo and reduce the rate of actin flux through actin networks. (rupress.org)

Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge. (wikipedia.org)

We found that, upon induction of apoptosis in control MEFs, n-cofilin, and its homolog ADF translocated to mitochondria together with actin. (nature.com)
In summary, our work demonstrates that in mammalian cells ADF/cofilin activity is not generally required for efficient induction of apoptosis. (nature.com)

In 2001, significant advances were made to our understanding of the structure and function of actin monomers. (edu.au)
Once freed, ADP-actin monomers can exchange ADP with cytoplasmic ATP, ready for reincorporation at the barbed end of a growing filament (Gungabissoon & Bamburg 2003). (reactome.org)

Overexpressing these mutant actins in mouse hippocampal neurons not only modulated growth-cone function but also neurite elongation, which was ambiguous by traditional pharmacological interference. (jneurosci.org)

Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. (lookformedical.com)

During cell migration, actin assembly drives cell membrane protrusion, while microtubules (MTs) extend within protrusions to promote adhesion site turnover. (ecmbio.com)

Cancer type-specific alterations in actin genes : worth a closer look? (ugent.be)

Non-muscle cofilin (n-cofilin) is a member of the ADF/cofilin family of actin depolymerizing proteins. (nature.com)
In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. (ecmbio.com)
Slingshot (SSH) is a member of the conserved family of cofilin phosphatases that plays a critical role in cell membrane protrusion and migration by transforming inactive phosphorylated cofilin to an active form. (oncotarget.com)
The filamin family of proteins bind to both actin and a number of signaling molecules including Rho GTPases. (mechanobio.info)
Neurotrophins bind two classes of receptors, the receptor tyrosine kinase family of Trk (A, B, and C) receptors and the p75 neurotrophin receptor (NTR). (jneurosci.org)
The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)

A synthesized peptide derived from human Actin-pan. (affbiotech.cn)

Actb , Acta1 ) and actin-binding proteins (e.g. (jneurosci.org)

In resting human being T cells cofilin is definitely inactive and exists in specific subcellular locations mainly. (ap26113.com)
Cytoplasmic cofilin is definitely phosphorylated and therefore within an inactive state mainly. (ap26113.com)
We have generated the LS180 cells expressing three different cofilin variants: WT (wild Palomid 529 (P529) type) Ser 3 Ala (S3A) (constitutively active) or Ser 3 Asp (S3D) (constitutively inactive cofilin). (healthcarecoremeasures.com)

Whether cofilin activity results in F-actin shrinking or enhanced polymerization depends on the conditions and availability of G-actin in the specific area within the cell 39 40 and is likely influenced by different signaling cascades. (ap26113.com)
Thus, we conclude that ADF/cofilin activity is not generally required for induction or progression of apoptosis in mammalian cells. (nature.com)
To test the relevance of ADF/cofilin activity for apoptotic processes, we here employed a genetically well-defined system of mouse embryonic fibroblasts (MEFs) devoid of any ADF/cofilin activity and challenged these cells with well-characterized apoptosis inducers. (nature.com)
Notably, a nucleus-resident actin (R62D NLS ) also regulates SRF's transcriptional activity. (jneurosci.org)
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. (thermofisher.com)
Additionally, we found that neurotrophin binding to p75 NTR reduces RhoA activity. (jneurosci.org)

Filamin forms a vital scaffolding adaptor and regulatory component that contributes to the mechanical stability of cells by linking the internal actin network with membrane receptors and mechanosensitive components. (mechanobio.info)

Anatomy6

Organisms2

Chemicals and Drugs27

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes12

Technology, Industry, Agriculture2

Information Science3

Filaments22

Cytoskeleton10

Protein15

Dynamics20

Phosphorylation8

Translocation7

Disassembly4

Serine1

Myosin1

Lamellipodial actin network1

Severs1

Vitro2

Isoforms4

Regulate actin1

Regulation of cofilin2

Plasma membrane1

Dephosphorylation7

Membrane-bound2

Mutants6

Microfilaments1

Polyclonal Antibody1

Proteins that regulate1

Cortical2

Lamellipodia1

Cytoplasm1

Mechanism3

Activates1

Kinetics2

Translocates1

Nucleation1

Vivo3

Arp21

Induction of apoptosis2

Monomers2

Growth-cone1

Structural1

Cell migration1

Genes1

Family6

Peptide1

Actb1

Inactive3

Activity6

Receptors1