CytoskeletalPhosphorylation of cofilinDepolymerizationCFL1DestrinBindsInhibitsProteinsLimk1InactivationDephosphorylationIsoformMitochondrialPhosphorylatesKinaseChromosomeMuscleResiduesELISA KitGenesCellsApoptosisDisassemblyVitroInteractsInhibitionMutationAntibodyResults demonstrateActivationHumanOxidationSymbolFamilyActivityBethesdaPituitaryMolecularFactorsGenerate
Cytoskeletal2
- Relevance for nursing practice: Genes including the actin cytoskeletal regulatory proteins Prokineticin 2 and Cofilin 2 along with mitochondrial inner membrane proteins are involved in multiple pathways and were predictors of fatigue status. (elsevierpure.com)
- In summary, we show for the first time an actin-microtubule cytoskeletal interplay mediated by cofilin-1 and MRTF-A/SRF, promoting the dilated cardiomyopathy caused by LMNA mutations. (helsinki.fi)
Phosphorylation of cofilin1
- Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1). (arigobio.cn)
Depolymerization8
- Deficiency of cofilin-2 may result in reduced depolymerization of actin filaments, causing their accumulation in nemaline bodies, minicores, and, possibly concentric laminated bodies. (wikipedia.org)
- It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. (nih.gov)
- Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. (arigobio.cn)
- The data indicate that cofilin oxidation almost eliminates its filaments depolymerization ability, and inhibits its binding to filaments. (nih.gov)
- AIP1(actin-interaction protein 1) is a 67 kD WD repeat protein known to regulate the depolymerization of actin filaments by cofilin and is conserved in organisms ranging from yeast to mammals. (biolegend.com)
- Recently, it was reported that caspase-11 and AIP1 cooperatively promote cofilin-mediated actin depolymerization. (biolegend.com)
- AIP1 binds cofilin/actin depolymerization factor. (biolegend.com)
- Caspase-11 interacts physically with AIP1 to promote cofilin-mediated actin depolymerization. (biolegend.com)
CFL14
- Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN), all of which regulate actin-filament dynamics. (wikipedia.org)
- Description: A sandwich quantitative ELISA assay kit for detection of Rat Cofilin 1, Non Muscle (CFL1) in samples from tissue homogenates or other biological fluids. (bioemm.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Cofilin 1, Non Muscle (CFL1) in Tissue homogenates and other biological fluids. (bioemm.com)
- LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. (arigobio.cn)
Destrin3
- The actual phosphorylation levels of cofilin-1 and also destrin were examined simply by 2-dimensional electrophoresis. (idoxuridinechemical.com)
- Beside the standard Rho/ROCK/MLC/myosin pathway, this particular walkway is a crucial element regarding amoeboid motility, distinct to the destrin isoform (rather than the coexpressed homologue cofilin-1). (idoxuridinechemical.com)
- Additionally, the existence of destrin (and not cofilin-1) in the p210(bcr-abl) sophisticated is dependent on ROCK1, this also signalplex is necessary for amoeboid motility. (idoxuridinechemical.com)
Binds4
- Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (wikipedia.org)
- Here, we show that in muscle cells carrying a cardiomyopathy-causing LMNA mutation, phospho(T25)-cofilin-1 binds to myocardin-related transcription factor A (MRTF-A) in the cytoplasm, thus preventing the stimulation of serum response factor (SRF) in the nucleus. (helsinki.fi)
- Rabbit polyclonal to COFILIN.Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulatingthe rapid cycling of Actin assembly and disassembly, essential for cellular viability. (bioshockinfinitereleasedate.com)
- Cofilin 1, alsoknown as Cofilin, non-muscle isoform, is a low molecular weight protein that binds to filamentousF-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filamenttwist. (bioshockinfinitereleasedate.com)
Inhibits2
- Inhibition of PLC inhibits cofilin activity in cells during the early transient, delays the initiation of protrusions, and inhibits the ability of cells to sense a gradient of EGF. (elsevierpure.com)
- Suppression of cofilin, using either small interfering RNA silencing or function-blocking antibodies, selectively inhibits the early transient. (elsevierpure.com)
Proteins7
- Oligomerization Affects the Ability of Human Cyclase-Associated Proteins 1 and 2 to Promote Actin Severing by Cofilins. (antibodies-online.cn)
- A member of the cofilin family of proteins that is expressed in MUSCLE CELLS . (bvsalud.org)
- 11R a) SSP referring to those numbers in Figure 2 and short protein names referring to those proteins listed in Table S2. (lu.se)
- HN - 2006(1998) MH - Actin-Related Protein 2-3 Complex UI - D051376 MN - D5.750.78.730.246 MN - D12.776.220.525.246 MS - A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. (nih.gov)
- Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
- Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)
- Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)
Limk14
- Combining Pgk1 incubation and Enolase-2 overexpression, we demonstrate a highly significant enhancement of neurite outgrowth of motoneurons through a reduced p-P38-T180/p-Limk1-S323/p-Cofilin signaling. (bvsalud.org)
- Collectively, extracellular Pgk1 interacts neural membrane receptor Enolase-2 to reduce the P38/Limk1/Cofilin signaling which results in promoting neurite outgrowth. (bvsalud.org)
- Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. (arigobio.cn)
- The blots were probed with anti-LIMK1 (C-terminus) (lanes 1 & 2) or anti-LIMK1 (Ser-323) (lanes 3 & 4). (phosphosolutions.com)
Inactivation1
- Our finding that Cys oxidation is not responsible for the inactivation of cofilin by TnCl suggests no role of actin dynamics in TnCl-induced apoptosis. (nih.gov)
Dephosphorylation1
- This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonizedzymosan. (bioshockinfinitereleasedate.com)
Isoform2
- Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on ActinFilaments Reveals Isoform-Specific Conformation and Binding Mode. (udel.edu)
- This specific emphasizes isoform-specific operate inside the ADF/cofilin loved ones and supplies brand-new comprehension of Bcr-Abl signaling to amoeboid motility as well as achievable effect on understanding long-term myeloid the leukemia disease progression. (idoxuridinechemical.com)
Mitochondrial2
- 2. Mitochondrial dysfunction and calcium deregulation by the RanBP9-cofilin pathway. (nih.gov)
- Many tumors exhibit an accompanying increase in mitochondrial metabolism associated with substantial CO 2 release ( 1 ). (aacrjournals.org)
Phosphorylates1
- We recently showed that ERK1/2 phosphorylates cofilin-1 on threonine 25 (phospho(T25)-cofilin-1) that in turn disassembles the actin cytoskeleton. (helsinki.fi)
Kinase2
- CIB1 overexpression significantly decreases cell migration on fibronectin as a result of a PAK1-and LIM kinase-dependent increase in cofilin phosphorylation. (rupress.org)
- L'analyse d'image des cartes appariées a permis d'identifier sept protéines qui étaient soit surexprimées, soit sous-exprimées : le récepteur de la protéine kinase C activée, la tubuline alpha (×2), la prostaglandine F2-alpha synthase, la protéine disulfide isomérase, une protéine du transport vésiculaire et une protéine hypothétique. (who.int)
Chromosome1
- chromosome 2 open reading frame 2. (gsea-msigdb.org)
Muscle2
- Cofilin 2 (muscle) also known as CFL2 is a protein which in humans is encoded by the CFL2 gene. (wikipedia.org)
- Description: A sandwich ELISA kit for detection of Cofilin 1, Non Muscle from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (bioemm.com)
Residues2
- TWF1 is composed of 2 ADF/cofilin-like (ADF-H) domains joined by a short linker region and followed by a C-terminal tail of approximately 20 residues. (prospecbio.com)
- Taurine chloramine (TnCl) oxidizes cysteine and methionine residues in cofilin, but only cysteine oxidation mediates TnCl-induced apoptosis (Nature Cell Biology 2009, v11, pp1241). (nih.gov)
ELISA Kit1
- Description: A sandwich ELISA kit for detection of Metallothionein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (agctsequencing.com)
Genes3
- magadii contained three genes en coding putative enzymes from the later on phases from the fol ate biosynthesis pathway. (cgrpreceptor.com)
- RT qPCR gene expression evaluation Expression of selected genes, for microarray validation and to more examine biological processes of curiosity, was studied by reverse transcription quantitative real time PCR , with target qPCR primer sequences offered in Extra file 2. (cgrpreceptor.com)
- On top of that, amplifi cation of two reference genes, cofilin two and elongation aspect 1, was carried out. (cgrpreceptor.com)
Cells5
- In fact, tumor cells maintain an alkaline intracellular pH (pH i ) relative to untransformed cells that permits unbridled proliferation ( 2 ). (aacrjournals.org)
- The loss of extracellular rigidity potentiates the increased motility of tumor cells with alkaline pH i due to the enhanced activity of the actin remodeling protein cofilin ( 2 ). (aacrjournals.org)
- Primary ocular cells will be cultured and treated with PAs for 5 hours 2. (uiowa.edu)
- In addition, we monitored transendothelial electrical resistance (TER) and protein expression in a monolayer of human lung microvascular endothelial cells (HLMVEC) exposed to HCl (0.02 N) and treated with vehicle or AT13387 (2 µM). (nih.gov)
- Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth. (nih.gov)
Apoptosis1
- 3. Pivotal role of the RanBP9-cofilin pathway in Aβ-induced apoptosis and neurodegeneration. (nih.gov)
Disassembly1
- Enhance actin filament disassembly in the presence of actin-depolymerizing factor cofilin. (biolegend.com)
Vitro2
- Local infusion of TAN-67, an in vitro -determined G i/o -protein biased DOR agonist, decreased voluntary alcohol intake in wild-type and β-arrestin-2 knockout (KO) mice. (frontiersin.org)
- Here we investigated the effect of TnCl-induced oxidation on filament actin regulatory activities of cofilin in vitro. (nih.gov)
Interacts1
- The 325th-417th domain of Pgk1 interacts with the 405th-431st domain of Enolase-2, but neither Enolase-1 nor Enolase-3, promoting neurite outgrowth. (bvsalud.org)
Inhibition1
- One potential AUD treatment approach is to increase inhibition of the dorsal striatum, a brain region with observed increasing activation upon alcohol tasting in heavy alcohol drinking human subjects ( 2 ). (frontiersin.org)
Mutation1
- In contrast to the previous finding from cellular studies, neither the reduction of intramolecular disulfide bonds nor the double Cys mutation restored the activities of oxidized cofilin, indicating the oxidation of Met rather than Cys as the main cause of its functional impairment. (nih.gov)
Antibody4
- For continuous use, store undiluted antibody at 2-8°C for up to a week. (arigobio.cn)
- The antibody solution should be stored undiluted between 2°C and 8°C. (biolegend.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Metallothionein 2 (MT2) in serum, plasma, tissue homogenates and other biological fluids. (agctsequencing.com)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Metallothionein 2 (MT2) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (agctsequencing.com)
Results demonstrate1
- Therefore, our results demonstrate that the early PLC and cofilin-dependent barbed end transient is required for the initiation of protrusions and is involved in setting the direction of cell movement in response to EGF. (elsevierpure.com)
Activation2
- Conversely, the RNA interference-mediated depletion of CIB1 increases cell migration and reduces normal adhesion-induced PAK1 activation and cofilin phosphorylation. (rupress.org)
- In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. (arigobio.cn)
Human1
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cofilin 2. (antibodies-online.cn)
Oxidation3
- HN - 2006(1983) MH - 2-Oxoisovalerate Dehydrogenase (Acylating) UI - D050645 MN - D8.811.682.657.350.825 MS - An NAD+ dependent enzyme that catalyzes the oxidation 3-methyl-2-oxobutanoate to 2-methylpropanoyl-CoA. (nih.gov)
- use ANTHRANILIC ACID 1974-1979 MH - 3-Isopropylmalate Dehydrogenase UI - D050539 MN - D8.811.682.47.500 MS - An NAD+ dependent enzyme that catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate to 3-carboxy-4-methyl-2-oxopentanoate. (nih.gov)
- Oxidation of Met-115 could cause the disruption of the globular-actin binding site, which is required for the function of cofilin under physiological salt conditions. (nih.gov)
Symbol1
- cofilin 2 [Source:HGNC Symbol;Acc. (gsea-msigdb.org)
Family1
- raftlin family member 2 [Source:H. (gsea-msigdb.org)
Activity1
- Mutations in the lamin A/C gene (LMNA) cause dilated cardiomyopathy associated with increased activity of ERK1/2 in the heart. (helsinki.fi)
Bethesda1
- 2 Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland. (nih.gov)
Pituitary2
- In the 2022 World Health Organization (WHO) classification of pituitary tumors, a major nomenclature change was introduced to refer to pituitary adenomas as PitNETs as the term adenoma refers to a benign disease that is not harmful to health or life which is inconsistent with the behaviors of pituitary tumors [ 2 ]. (e-enm.org)
- Pituitary adenylate cyclase-activating polypeptide/vasoactive intestinal peptide (Part 2): biology and clinical importance in central nervous system and inflammatory disorders. (nih.gov)
Molecular1
- Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp23 complex pathway and cofilin. (forexinfolink.com)
Factors1
- The abruptly of the three largest values of the respunk index of 5 factors old (2 of 5 miniest indices were excluded) settle ons the value of the CR in each case in Table 1. (ika.ie)
Generate2
- 9/3/2005) TOTAL DESCRIPTORS = 935 MH - 1-Acylglycerol-3-Phosphate O-Acyltransferase UI - D051103 MN - D8.811.913.50.173 MS - An enzyme that catalyzes the acyl group transfer of ACYL COA to 1-acyl-sn-glycerol 3-phosphate to generate 1,2-diacyl-sn-glycerol 3-phosphate. (nih.gov)
- HN - 2006(1981) MH - 2-Aminoadipate Transaminase UI - D051307 MN - D8.811.913.477.700.120 MS - A PYRIDOXAL PHOSPHATE containing enzyme that catalyzes the transfer of amino group of L-2-aminoadipate onto 2-OXOGLUTARATE to generate 2-oxoadipate and L-GLUTAMATE. (nih.gov)