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  • snoRNAs
  • Oocyte and HeLa nuclear extracts are able to process X. laevis and human substrates in a similar manner, indicating that the processing of this class of snoRNAs relies on a common and evolutionarily conserved mechanism. (asm.org)
  • regulate
  • It is safe to assume that the microRNA network is not likely to be the last example demonstrating how organisms have developed ingenious ways to regulate biological process via RNA sequences. (pubmedcentralcanada.ca)
  • play important roles
  • Together with high‐throughput biochemical characterization of dmDis3l2 and bacterial RN ase R, our results imply a conserved molecular function of RN ase II /R enzymes as "readers" of destabilizing posttranscriptional marks-uridylation in eukaryotes and adenylation in prokaryotes-that play important roles in RNA surveillance. (embopress.org)
  • cellular
  • To monitor Ψ levels quantitatively, a previously reported high performance liquid chromatography method coupled with ultraviolet detection (HPLC-UV) was modified to determine total Ψ levels in different cellular RNA fractions. (mdpi.com)
  • Unlike prokaryotes, eukaryotic cells segregate the vast majority of their RNA and protein synthesis into two distinct cellular compartments, i.e. the nucleus and the cytoplasm. (biologists.org)
  • The bulk of cellular nucleocytoplasmic transport is mediated by factors that belong to a single family of nuclear transport receptors termed karyopherins or importins/exportins. (biologists.org)
  • Corroborating the result of computational analyses, deletion of introns from three essential genes decreased cellular RNA levels and caused measurable growth defects. (genetics.org)
  • polymerases
  • To validate and confirm new ncRNA candidates, we adapted an iterative strategy combining both genome-wide occupancy of the different subunits of RNA polymerases I, II and III and expression data. (biomedcentral.com)
  • shRNAs
  • More recently, researchers have identified the human 7SK promoter as the most efficient pol III promoter for the expression of short hairpin RNAs (shRNAs) in vivo, surpassing the activity of the human U6-1 promoter (Koper-Emde et al, 2004). (alliedacademies.org)
  • exons
  • Sequencing of coding exons of all genes in the region identified multiple segregating variants in one gene, recently annotated as serine protease gene ( PRSS56 ), coding for a predicted trypsin serine protease-like protein. (molvis.org)
  • molecular
  • Nucleobases provide the molecular structure necessary for the hydrogen bonding of complementary DNA and RNA strands, and are key components in the formation of stable. (absoluteastronomy.com)
  • transcript
  • we have now validated the structure of a full-length transcript from embryonic mouse brain RNA. (molvis.org)
  • novel
  • Aravin A, Gaidatzis D, Pfeffer S et al (2006) A novel class of small RNAs bind to MILI protein in mouse testes. (springer.com)
  • S.P. Nana-Sinkam, C.M. Croce, Non-coding RNAs in cancer initiation and progression and as novel biomarkers. (springer.com)
  • In European honeybees ( Apis mellifera ), we identified a novel nuclear non-coding RNA, termed kakusei , as the first insect IEG, and revealed the neural activity pattern in foragers. (mdpi.com)
  • RNAi
  • The identification and characterisation of the porcine RNA pol III promoter will contribute to the area of RNAi delivery and further develop our understanding of RNA promoter structure and function. (alliedacademies.org)
  • pseudouridine
  • Aravind L, Koonin EV (2001b) THUMP-a predicted RNA-binding domain shared by 4-thiouridine, pseudouridine synthases and RNA methylases. (springer.com)
  • nucleotide
  • A mistake in even a single nucleotide can be devastating to the cell, and a reliable, repeatable method of RNA processing is necessary to ensure cell survival. (wikipedia.org)
  • known
  • All non-coding RNAs are now known to depend on members of the karyopherin family of Ran-dependent nucleocytoplasmic transport factors for their nuclear export. (biologists.org)
  • Little is known about the mechanism and the factors involved in this new type of processing. (asm.org)