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  • cells
  • Also, Wnt3A inhibited B lymphopoiesis of CD133+CD10- hematopoietic progenitor cells and CD10+ B progenitor cells in coculture assays using a supportive layer of stromal cells. (biomedsearch.com)
  • Examination of early events in the coculture showed that Wnt3A inhibits cell division of B progenitor cells. (biomedsearch.com)
  • The possibilities and limitations of the most useful liver in vitro systems are summarized, including three-dimensional culture techniques, co-cultures with non-parenchymal cells, hepatospheres, precision cut liver slices and the isolated perfused liver. (nih.gov)
  • A neutralizing monoclonal antibody against epimorphin (MC-1) efficiently blocked the development of hollow sphere structures from A818-6 cells.Coculture of A818-6 cells with fibroblasts stimulated the development of hollow sphere structures in general and increased differentiation in 5-6-d-old hollow spheres.A818-6 hollow sphere development in the presence of fibroblasts was also blocked by MC-1. (nih.gov)
  • Coculture of A818-6 cells with fibroblasts stimulated the development of hollow sphere structures in general and increased differentiation in 5-6-d-old hollow spheres. (nih.gov)
  • Confocal laser scanning analysis of coculture experiments of A818-6 cells with Kif-5 fibroblasts transduced with the gene coding for the EGFP. (nih.gov)
  • Fibroblast cocultures with A818-6 cells were set up using foreskin-derived KIF-5 fibroblasts. (nih.gov)
  • Using biochemical selection methods, we found exponentially growing cells in restrictive media (uridine(-) and bromodeoxyuridine [BrdU](+)) during the coculture of MSCs (uridine-independent and BrdU-sensitive) and 143B-derived cells with severe mitochondrial dysfunction induced by either long-term ethidium bromide treatment or short-term rhodamine 6G (R6G) treatment (uridine-dependent but BrdU-resistant). (nih.gov)
  • We cocultured MSCs with 143B-derived cells with severely compromised mitochondrial function (Δmt) in uridine− BrdU− media for five days (Stage I) and in uridine− BrdU+ media thereafter (Stage II). (nih.gov)
  • We cocultured human bone marrow derived MSCs at 1.5×105 cells with those cells listed above (i.e., 143B ρ0, cybrids with A3243G, and cybrids with 4,977 bp deletion) at 3×105 cells in a 100 mm culture dish with DMEM supplemented with 10% FBS and 50 µg/ml uridine for 24 h. (nih.gov)
  • A variety of techniques has been developed for the isolation and culturing of amniotic stem cells. (wikipedia.org)
  • Mesenchymal stem cells (MSCs) are highly abundant in the amniotic fluid and several techniques have been described for their isolation. (wikipedia.org)
  • In 2016, researchers developed a technique that used cancer cells' RNA to produce T cells and an immune response. (wikipedia.org)
  • experiments
  • Scheme of coculture experiments.Mitochondrial dysfunction was induced by long-term treatment of ethidium bromide (EtBr), which is known to remove mtDNA while sparing nuclear DNA, or by short-term treatment of rhodamine 6G (R6G), which tightly binds to the mitochondrial inner membrane and destroys the mitochondrial respiratory function. (nih.gov)
  • Intracytoplasmic Sperm Inj
  • He developed a precursor technique of Intracytoplasmic Sperm Injection (ICSI), which is now used for treatment of nearly all male factor infertility diagnoses. (wikipedia.org)
  • Techniques such as intracytoplasmic sperm injection (ICSI) can also be utilised to improve the chances of fertilisation being successful, which should be performed at least one hour (and optimally two to four hours) after the first polar body extrusion. (wikipedia.org)
  • human
  • Novel multicolor fluorescence painting techniques of human chromosomes have clearly demonstrated that high-LET α-particles and Fe-ions induce many more complex rearrangements will ultimately lead to cell death. (wikipedia.org)
  • proteins
  • Analysis of the cell cycle-associated proteins cyclin B and p27/Kip after coculture revealed a downregulation of p27/Kip and a weak upregulation of cyclin B in A818-6 hollow spheres (Fig. 4 B, lane 3). (nih.gov)
  • In addition, the production of the various organic macromolecules of interest (proteins, lipids, starch) differ depending on the technique used to create biomass and can be therefore targeted. (wikipedia.org)
  • early
  • Early studies in the 80's, led by Mina Bissell from the Lawrence Berkeley National Laboratory, highlighted the importance of 3D techniques for creating accurate in vitro culturing models. (wikipedia.org)
  • The technique for growing organoids has rapidly improved since the early 2010s, and it was named by The Scientist as one of the biggest scientific advancements of 2013. (wikipedia.org)
  • study
  • Genome-based analytical techniques may prove especially useful for L. anisa, as a study of isolates from various locations in France suggest that the genomic variation is much more limited and homogenous than other Legionella species. (wikipedia.org)
  • As the experiment has continued, its scope has grown as new questions in evolutionary biology have arisen that it can be used to address, as the populations' evolution has presented new phenomena to study, and as technology and methodological techniques have advanced. (wikipedia.org)
  • breast
  • As of 2015 the technique had expanded to treat cervical cancer, lymphoma, leukemia, bile duct cancer and neuroblastoma and in 2016, lung cancer, breast cancer, sarcoma and melanoma. (wikipedia.org)
  • formation
  • Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. (nih.gov)
  • To further investigate the contribution of OL myosin II on myelin formation, we infected OPC-DRG cocultures with shRNA lentivirus against the regulatory chain of MLC. (nih.gov)
  • similar
  • Knockdown of MLC in OPC-DRG cocultures resulted in a significant increase in myelination similar to that observed in cultures treated with blebbistatin. (nih.gov)
  • known
  • Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. (wikipedia.org)
  • A. thiooxidans is used in the mining technique known as bioleaching, where metals are extracted from their ores through the action of microbes. (wikipedia.org)
  • fertilisation
  • Techniques such as intracytoplasmic sperm injection (ICSI) can also be utilised to improve the chances of fertilisation being successful, which should be performed at least one hour (and optimally two to four hours) after the first polar body extrusion. (wikipedia.org)
  • tissue
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • made
  • Here we used several metabolomic approaches to examine the chemicals made by these bacteria when grown in pairwise coculture. (asm.org)
  • interest
  • In addition, the production of the various organic macromolecules of interest (proteins, lipids, starch) differ depending on the technique used to create biomass and can be therefore targeted. (wikipedia.org)
  • finding new
  • These findings suggest that chemical interactions between actinomycetes are surprisingly complex and that coculture may be a promising strategy for finding new molecules from actinomycetes. (asm.org)