Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure. (cdc.gov)
This study identified differential gene regulation in human small airway epithelial cells (SAECs) grown either in monoculture or in coculture with human microvascular endothelial cells following exposure of the SAECs to multiwalled carbon nanotube s (MWCNTs). (cdc.gov)
Assay1
Coculture assay further confirmed the attractive effect of sensory axons on sympathetic axons. (jneurosci.org)
Endothelial1
This study aimed to establish and characterize an advanced respiratory model consisting of human epithelial bronchial BEAS-2B cells cultivated at the air-liquid interface (ALI), both as monocultures and in cocultures with human endothelial EA.hy926 cells. (nilu.no)
PSCs2
We investigated the role of Echinococcus multilocularis (Echinococcus genus) PSCs in hepatic fibrosis by examining structural changes and measuring hepatic fibrosis-related protein levels in cocultures of PSCs and human HSCs. (biomedcentral.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
Monoculture4
The performance of the bronchial models was compared to a commonly used alveolar model consisting of A549 in monoculture and in coculture with EA.hy926 cells. (nilu.no)
We performed an inter-laboratory comparison study to evaluate the transferability and reproducibility of a lung model consisting of the human bronchial cell line Calu-3 as a monoculture and, to increase the physiologic relevance of the model, also as a coculture with macrophages (either derived from the THP-1 monocyte cell line or from human blood monocytes). (vitrocell.com)
SAEC genes that either changed their regulation direction from upregulated in monoculture to downregulated in coculture (or vice versa) or had a more than a two-fold changed in the same regulation direction were identified. (cdc.gov)
The direction and fold-change of this differential gene regulation suggests that toxicity testing in monoculture may exaggerate cellular responses to MWCNTs, and coculture of cells may provide a more in-depth assessment of toxicological responses. (cdc.gov)
VIVO2
The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
While single-cell models of pulmonary exposure are often used to determine the potential toxicity of nanomaterial s in vitro, previous studies have shown that coculture cell models better represent the cellular response and crosstalk that occurs in vivo. (cdc.gov)
Versus1
the rates (fraction) reaching blastocyst stage were 56% versus 46% in the coculture versus the sequential system, respectively, with own oocytes. (wikipedia.org)
Endothelial cells2
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure. (cdc.gov)
This study identified differential gene regulation in human small airway epithelial cells (SAECs) grown either in monoculture or in coculture with human microvascular endothelial cells following exposure of the SAECs to multiwalled carbon nanotube s (MWCNTs). (cdc.gov)
Amoebal Coculture5
Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human pathogens. (cdc.gov)
To isolate new ARB, we performed amoebal coculture from 444 nasal samples. (cdc.gov)
Thus, amoebal coculture allows the recovery of new bacterial species from heavily contaminated samples and might be a valuable approach for the recovery of as-yet unrecognized emerging pathogens from clinical specimens. (cdc.gov)
Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB) and clean the samples of other more rapidly growing species that generally overwhelm the agar plates. (cdc.gov)
We tried to recover ARB and free-living amoebae by using amoebal coculture and amoebal enrichment, respectively, with any recovered amoebae subsequently examined for the presence of ARB. (cdc.gov)
Fermentation3
Studies on simultaneous saccharification and fermentation (SSF) of wheat bran flour, a grain milling residue as the substrate using coculture method were carried out with strains of starch digesting and nonstarch digesting and sugar fermenting in batch fermentation. (engineering.org.cn)
Klebsiella pneumonia , Enterobacter cloacae , and their coculture were used to synthesize biohydrogen using Oscillatoria acuminata biomass via dark fermentation. (biomedcentral.com)
These results confirm the effectiveness of biological treatments + Mg-Zn Fe 2 O 4 -NPs and coculture dark fermentation in upregulating biohydrogen production. (biomedcentral.com)
Significantly1
Biological pretreatment + Mg-Zn Fe 2 O 4 NPs using coculture significantly stimulated hydrogen yield (3254 mLH 2 /L), hydrogen efficiency)216.9 mL H 2 /g reducing sugar( and hydrogen production rate (67.7 mL/L/h) to the maximum among all pretreatments. (biomedcentral.com)
Vitro2
The engineered coculture platform lays groundwork toward precision medicine wherein patient-derived tumor cells can be incorporated within our in vitro models to identify potential pathways and drug treatment regimens for individual patients. (unl.edu)
While single-cell models of pulmonary exposure are often used to determine the potential toxicity of nanomaterial s in vitro, previous studies have shown that coculture cell models better represent the cellular response and crosstalk that occurs in vivo. (cdc.gov)
Subsequently1
Numerous studies have explored engineering coculture of tumor and stromal cells predominantly using transwell coculture of stromal cells cultured onto coverslips that were subsequently added to tumor cell cultures. (unl.edu)
Cells4
Breast Cancer/Stromal Cells Coculture on Polyelectrolyte Films Emulat" by Amita Daverey, Karleen M. Brown et al. (unl.edu)
To our knowledge, this is the first demonstration of patterned coculture of tumor cells and stromal cells that captures the temporal changes in the miRNA signature as the breast tumor develops through various stages. (unl.edu)
SPS patterns were formed on PEM surfaces, by either capillary force lithography (CFL) of SPS onto PDAC surfaces or vice versa, to obtain patterns of breast cancer cells and patterned cocultures of breast cancer and stromal cells. (unl.edu)
The direction and fold-change of this differential gene regulation suggests that toxicity testing in monoculture may exaggerate cellular responses to MWCNTs, and coculture of cells may provide a more in-depth assessment of toxicological responses. (cdc.gov)
Model3
The coculture model mimics the proliferative index (Ki67 expression) and tumor aggressiveness (HER-2 expression) akin to those observed in clinical tumor samples. (unl.edu)
We also demonstrated that our patterned coculture model captures the temporal changes in the miRNA-21 and miRNA-34 signature as the breast tumor develops through various stages. (unl.edu)
Coculture model of blood-brain barrier on electrospun nanofibers" by MUSTAFA GÖRKEM ÖZYURT, ECE BAYIR et al. (tubitak.gov.tr)