• Searching for up to 100 curated homologs for Pf1N1B4_5133 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9) (394 a.a. (lbl.gov)
  • Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1. (lbl.gov)
  • Acetoacetyl-CoA thiolase (also called thiolase II) is specific for the thiolysis of acetoacetyl-CoA and involved in biosynthetic pathways such as poly β-hydroxybutyrate synthesis or steroid biogenesis. (expasy.org)
  • Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase. (expasy.org)
  • [4] Trifunctional protein deficiency is characterized by decreased activity of long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD), long-chain enoyl-CoA hydratase, and long-chain thiolase. (wikidoc.org)
  • The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. (nih.gov)
  • Roles of hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha, a lipid metabolism enzyme, in Wilms tumor patients. (nih.gov)
  • Trifunctional enzyme subunit beta, mitochondrial (TP-beta) also known as 3-ketoacyl-CoA thiolase , acetyl-CoA acyltransferase , or beta-ketothiolase is an enzyme that in humans is encoded by the HADHB gene . (wikidoc.org)
  • HADHB is a subunit of the mitochondrial trifunctional protein and has thiolase activity. (wikidoc.org)
  • beta-ketoacyl-CoA thiolase, anaerobic, subunit. (ntu.edu.sg)
  • 3-Ketoacyl-CoA thiolase, mitochondrial also known as acetyl-Coenzyme A acyltransferase 2 is an enzyme that in humans is encoded by the ACAA2 gene. (wikipedia.org)
  • Similarity between the amino-terminal portion of mammalian 58-kD sterol carrier protein (SCPx) and Escherichia coli acetyl-CoA acyltransferase: evidence for a gene fusion in SCPx. (expasy.org)
  • Fecal specimens collected at baseline and at week 72 after treatment completion (FUw72) were analyzed for 16S rRNA sequencing and the butyryl-CoA:acetateCoA transferase (BCoAT) gene expression using real-time PCR. (bvsalud.org)
  • 31785) acetyl-CoA carboxylase%2C biotin carboxylase CP001857 CDS Arcpr_0038 complement(31809. (go.jp)
  • Primary structure of 3-ketoacyl-coenzyme A thiolase from Escherichia coli and the structural organization of the fadAB operon. (expasy.org)
  • Background: Succinyl-CoA:3 oxoacid CoA transferase deficiency (SCOTD) is a rare autosomal recessive disease, characterized by altered utilization of ketone bodies, with acute episodes of ketoacidosis. (bvsalud.org)
  • The HADHB protein catalyzes the final step of beta-oxidation, in which 3-ketoacyl CoA is cleaved by the thiol group of another molecule of Coenzyme A . The thiol is inserted between C-2 and C-3, which yields an acetyl CoA molecule and an acyl CoA molecule, which is two carbons shorter. (wikidoc.org)
  • 3-ketoacyl-CoA synthase n=1 Tax=Physcomitrella patens subsp. (uma.es)
  • 3-ketoacyl-CoA thiolase OS=Klebsiella pneumoniae subsp. (uu.nl)
  • 3-ketoacyl-CoA thiolase OS=Aeromonas hydrophila subsp. (uu.nl)
  • To be available for TAG synthesis, carbons from sucrose must first be converted to pyruvate through cytosolic or plastidic glycolytic pathways and at some point be imported into the plastid to yield acetyl-coenzyme A (CoA) which feeds fatty acid (FA) synthesis with carbon backbones. (biomedcentral.com)
  • Functional annotation resulted in the identification of almost all genes involved in diverse lipid-metabolic pathways, including the novel fatty acid desaturase/epoxygenase, diacylglycerol acyltransferase 1 and 2, and phospholipid:diacylglycerol acyltransferases. (researchsquare.com)
  • 3-ketoacyl-CoA thiolase OS=Marinomonas sp. (uu.nl)
  • There are two conserved cysteine residues important for thiolase activity. (expasy.org)
  • In eukaryotes, there are two forms of 3-ketoacyl-CoA thiolase: one located in the mitochondrion and the other in peroxisomes. (expasy.org)
  • Phylogenetic analysis of the thiolase family. (expasy.org)
  • Acyl-CoA movement into the mitochondrion is a crucial factor in regulation. (ufp.pt)
  • Malonyl-CoA (which is present in the cytoplasm in high amounts when metabolic fuels are abundant) inhibits carnitine acyltransferase, thereby preventing acyl-CoA from entering the mitochondrion. (ufp.pt)
  • This step uses two high energy phosphate and successfully converts fatty acid into acetyl CoA The purpose of acyl CoA synthetase is to trap fatty acid within the cell.Fatty acids are nonpolar substances. (prepladder.com)
  • Acyl CoA with carnitine gives acylcarnitine, and the enzyme is carnitine acyl transferase 1 (rate-limiting enzyme). (prepladder.com)
  • Carnitine acyltransferase 2 releases the carnitine back and converts acylcarnitine to acyl CoA. (prepladder.com)
  • CoA is a coenzyme of acyltransferase reactions and is important in the formation of acyl-CoA compounds. (coalitionbrewing.com)
  • Acyl-CoA and acetyl-CoA should not be confused with each other. (amboss.com)
  • Acetyl-CoA is the acyl-CoA of acetic acid (also known as acetate). (amboss.com)
  • Here we provide a detailed procedure for using DTNB to measure BAHD acyl-CoA acyltransferase reaction rates, and as an example, use it to determine kinetic parameters for red clover hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase, a BAHD acyl-CoA hydroxycinnamoyltransferase not previously characterized with respect to kinetic parameters. (bvsalud.org)
  • 3-Ketoacyl-CoA thiolase, mitochondrial also known as acetyl-Coenzyme A acyltransferase 2 is an enzyme that in humans is encoded by the ACAA2 gene. (wikipedia.org)
  • Mitochondrial acetoacetyl-CoA thiolase (T2, encoded by the ACAT1 gene) deficiency is an inherited disorder of ketone body and isoleucine metabolism. (nih.gov)
  • deficiency of mitochondrial acetoacetyl-coenzyme A thiolase T2/ "beta-ketothiolase") is an autosomal recessive disorder of ketone body utilization and isoleucine degradation due to mutations in ACAT1. (nih.gov)
  • In OA-loaded HepaRG cells, FOH increased fatty acid oxidation, which was accompanied by up-regulation of PPARα target genes involved in mitochondrial fatty acid oxidation, including hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase and acetyl-coenzyme A acyltransferase 2. (nih.gov)
  • As soon as fatty acid gets into any cell, irrespective of the final fate of fatty acid, the 1st enzyme to act on this fatty acid will be acetyl CoA synthetase. (prepladder.com)
  • This acetyl CoA synthetase uses 1 ATP which gets converted to AMP + PPI. (prepladder.com)
  • Step 2: Conversion of Acetyl-CoA to CoA - In this step, the acetyl-CoA formed in the previous step is converted into an active form of CoA by the enzyme known as Acetyl-CoA synthetase. (coalitionbrewing.com)
  • Medium-chain ketoacyl-CoA thiolase deficiency is a disorder associated with vomiting, dehydration, metabolic acidosis, liver dysfunction, and rhabdomyolysis. (nih.gov)
  • The cause of medium-chain ketoacyl-CoA thiolase deficiency is unknown. (nih.gov)
  • Acetyl-Coenzyme A acyltransferase 2 is an acetyl-CoA C-acyltransferase enzyme. (wikipedia.org)
  • An enzyme that catalyzes the formation of acetoacetyl-CoA from two molecules of ACETYL COA . (nih.gov)
  • Enzyme that catalyzes the final step of fatty acid oxidation in which ACETYL COA is released and the CoA ester of a fatty acid two carbons shorter is formed. (bvsalud.org)
  • Pantethine then reacts with ATP and the enzyme pantetheine kinase to form CoA, which is the end product. (coalitionbrewing.com)
  • This reaction is catalyzed by an enzyme known as Thiolase. (coalitionbrewing.com)
  • Mct catalyzes intra-molecular CoA transfers at catalytic efficiencies that are at least more than 6 orders of magnitude higher compared to inter-molecular CoA transfers, demonstrating that the enzyme exhibits exquisite control over its reaction. (bvsalud.org)
  • To understand the molecular basis of the intra-molecular CoA transfer in Mct, we solved crystal structures of the enzyme from Chloroflexus aurantiacus in its apo form, as well as in complex with mesaconyl-CoA and several covalently enzyme-bound intermediates of CoA and mesaconate at the catalytically active residue Asp165. (bvsalud.org)
  • Body Structure C99147 Neonatal Research Network Terminology C61262 Sanfilippo Syndrome Acetyl-CoA Acyltransferase Deficiency A rare autosomal recessive lysosomal storage disease affecting the metabolism of mucopolysaccharides. (nih.gov)
  • fatty acid β-oxidation - fatty acids breakdown into acetyl-CoA, to be used by the Krebs' cycle. (ufp.pt)
  • Acetyl-CoA is formed after glycolysis when pyruvate from the glycolytic pathway is transported into the mitochondria and converted into acetyl CoA. (coalitionbrewing.com)
  • This acetyl group is made up of a carbon (C) and two hydrogen (H) atoms, which have been taken from pyruvate. (coalitionbrewing.com)
  • During the conversion of pyruvate to acetyl CoA, electrons are also transferred to the electron transport chain, which is responsible for generating the majority of the energy in cells. (coalitionbrewing.com)
  • How does pyruvate become acetyl-CoA? (coalitionbrewing.com)
  • Step 1: Oxidation of Pyruvate - In this step, pyruvate is oxidized and a carbon dioxide molecule is released, while the remaining two-carbon molecule is converted into Acetyl-CoA. (coalitionbrewing.com)
  • Two molecules of acetyl CoA are produced from each glucose molecule, and these molecules of acetyl coA are then passed into the electron transport chain. (coalitionbrewing.com)
  • In the electron transport chain, acetyl coA is combined with oxygen to form two molecules of water and the energy-carrying molecule called ATP. (coalitionbrewing.com)
  • During beta-oxidation, two-carbon molecules are removed from the fatty acids, and these two-carbon molecules are combined to form one molecule of Acetyl CoA. (coalitionbrewing.com)
  • Acetyl-CoA is a key molecule involved in the metabolism of carbohydrates, fats, and proteins. (coalitionbrewing.com)
  • When the acetic acid gets attached to coA, we will call it acetyl CoA. (prepladder.com)
  • like citrate synthase, these are inhibited by NADH and succinyl-CoA. (ufp.pt)
  • This approach may be more generally applicable to transferases using CoA donors. (bvsalud.org)
  • This acetyl CoA then enters the Kreb's cycle and the product of ATP is the same as when Acetyl coA is produced from carbohydrates. (coalitionbrewing.com)
  • Acetyl CoA is produced from the metabolism of carbohydrates, proteins, and fats in a process called cellular respiration. (coalitionbrewing.com)
  • CoA is also involved in acyl group transfers, and these reactions are important in many metabolic processes, including the synthesis of ketones, amino acids and neurotransmitters. (coalitionbrewing.com)
  • BAHD acyl-coenzyme A (CoA) acyltransferases play key roles in a large number of biosynthetic reactions involved in plant specialized metabolism. (bvsalud.org)
  • We previously developed and validated an alternative approach using 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB, Ellman's reagent) to spectrophotometrically monitor reaction progress by the release of free CoA in the reaction. (bvsalud.org)
  • Here, we show that the reaction of Mct proceeds without any significant release of free CoA or the transfer to external acceptor acids. (bvsalud.org)
  • As such, CoA is essential for many cellular processes, including energy production. (coalitionbrewing.com)
  • Step 3: Conversion of CoA to Acetyl-CoA - In this final step, the CoA that was previously generated is converted back into the active form of Acetyl-CoA. (coalitionbrewing.com)