also, measured were medium chain acyl-CoA dehydrogenase (MCAD) and citrate synthase activities plus uncoupling protein 3 (UCP3), mitochondrial thioesterase 1 (MTE-1), adenine nucleotide translocase (ANT) and ATP synthase protein levels. (ox.ac.uk)
MenH (SHCHC synthase) was previously thought to be a thioesterase involved in hydrolyzing DHNA-CoA in a later step of menaquinone synthesis. (wikipedia.org)
In 2008, it was determined that MenH has poor catalytic activity toward palmitoyl-CoA, casting doubt on its role as a thioesterase. (wikipedia.org)
In 2009, it was proposed that a dedicated hotdog fold thioesterase would be needed to catalyze the hydrolysis of DHNA-CoA. (wikipedia.org)
mouse-adcl3 Mus musculus (Mouse) Arylacetamide deacetylase-like 3 , mouse-adcl4 Mus musculus (Mouse) Arylacetamide deacetylase-like 4 Hypothetical esterase/lipase/thioesterase family active site containing protein , mouse-aryla Mus musculus (Mouse) arylacetamide deacetylase and 5033417e09rik protein , mouse-Q8BLF1 Mus musculus (Mouse) KIAA1363 NCEH1 neutral cholesterol ester hydrolase 1 B230106I24RIK , mouse-b1avu7 Mus musculus (Mouse). (inra.fr)
Palmitoyl protein thioesterase 1(PPT1) is a lysosomal enzyme that catalyzes the protein depalmitoylation. (bvsalud.org)
Coenzyme3
It catalyzes the hydrolysis of long chain fatty acyl thioesters of acyl carrier protein or coenzyme A to form free fatty acid and the corresponding thiol: palmitoyl-CoA + H2O = CoA + palmitate It has a strict specificity for thioesters with a chain link greater than C10. (wikipedia.org)
W. using the condensation of palmitoyl coenzyme A (CoA) and serine by serine palmitoyltransferase (SPT), an enzyme that's negatively governed by ORM1-like proteins 3 (ORMDL3). (moonphase2018.com)
The NAD+ reduction was measured spectrophotometrically at 340 nm for 5 min by addition of 0.01 mM palmitoyl-CoA to the assay mixture containing 47 mM Tris·HCl (pH 8.0) 0.2 mM NAD+ 1 mM dithiothreitol 0.0075% (wt/vol) bovine serum albumin 0.01% (wt/vol) Triton X-100 0.1 mM coenzyme A 0.01 mM flavin adenine dinucleotide and 1 mM KCN. (cancerhugs.com)
Protein4
Leptin Suppresses Stearoyl-CoA Desaturase 1 by Mechanisms Independent of Insulin and Sterol Regulatory Element-Binding Protein-1c. (fireinabottle.net)
These six ACBPs map to four distinct classes based on the presence of other protein domains besides the conserved acyl-CoA-binding domain. (aocs.org)
FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
Synthetase2
Early investigations were focused on the 10-kDa ACBPs from mammals and yeast, and from these studies the 10-kDa ACBP has been implicated in biological roles ranging from acyl-CoA transport, maintenance of acyl-CoA pools within the cell and in the protection of acyl-CoAs in the cytosol from enzymes including acyl-CoA hydrolase, acetyl-CoA carboxylase and acyl-CoA synthetase [reviewed in 3]. (aocs.org)
According to available reports this enzymes are effective in regulating of intracellular concentration of acyl coa, fatty acid and co enzyme. (journalcra.com)
Serine1
Serine lipid hydrolase associated with lipid droplets. (swisspalm.org)
Palmitate1
Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. (thermofisher.com)
Synthase1
SHCHC synthase forms an alpha/beta hydrolase fold with a central set of seven parallel beta sheets surrounded by alpha helixes on both sides. (wikipedia.org)
PROTEINS2
In plants cells, lipid-transfer proteins (LTPs) and acyl-CoA-binding proteins (ACBPs) have been identified as candidates for lipid transfer within the cell [reviewed in 1,2]. (aocs.org)
Currently, information on these larger proteins with acyl-CoA-binding domains is available from data mining of sequenced genomes. (aocs.org)
Endoplasmic1
A thorough examination of saposin D-mutated PD patient-derived cells from the Japanese patients reported endoplasmic reticulum PSAP retention but preserved sphingolipid hydrolase activity, which is inconsistent with the canonical theory of PSAP and saposins 7 . (nature.com)
Enzymes1
While the details of the mechanism are not known, the results suggest that there is an "induction" mechanism taking place for palmitoyl-CoA hydrolase and peroxisomal beta-oxidation enzymes. (wikipedia.org)
Palmityl2
Palmityl-CoA, also known as palmitoyl CoA or CoA, palmitoyl, belongs to the class of organic compounds known as long-chain fatty acyl coas. (ymdb.ca)
Palmityl-CoA is a strong basic compound (based on its pKa). (ymdb.ca)
Arabidopsis2
The function of the acyl-CoA-binding domain in recombinant Arabidopsis ACBPs expressed in the bacterium Escherichia coli was established using site-directed mutagenesis followed by in vitro binding assays. (aocs.org)
Given that recombinant Arabidopsis ACBPs show preferences for different acyl-CoA esters and phospholipids in in vitro binding assays, it was hypothesized that members in the Arabidopsis ACBP family have nonoverlapping functions in the plant cell. (aocs.org)
This led to high levels of fatty acyl-CoA being present in the liver, which shows that a diseased liver cannot regulate the amount of fatty acyl-CoA that is present versus a normal, healthy liver. (wikipedia.org)
no significant changes were noted in liver aminopyrine - N - demethylase or aniline hydrolase activities. (cdc.gov)
Quantitative Proteome Analysis of Mouse LiverLysosomes Provides Evidence for Mannose 6-phosphate-independent Targeting Mechanisms of Acid Hydrolases in Mucolipidosis II. (uni-bielefeld.de)
Fatty acid2
In the enzymology, the palmitoyl - co a hydrolase is said to the enzyme that hydrolase the fatty acid. (journalcra.com)
Role for fatty acid amide hydrolase (FAAH) in the leptin-mediated effects on feeding and energy balance. (fireinabottle.net)
Peroxisome1
Enzymatic indicators of peroxisome proliferation (KCN-insensitive palmitoyl-CoA oxidation or enoyl-CoA hydratase heat labile activity) were not significantly altered compared to controls. (cdc.gov)
Enzyme that catalyzes1
An enzyme that catalyzes reversibly the conversion of palmitoyl-CoA to palmitoylcarnitine in the inner mitochondrial membrane. (lookformedical.com)
Assay1
Recombinant ACBPs have been tested and shown to bind acyl-CoA esters in vitro using binding assays such as the Lipidex assay. (aocs.org)
Acyltransferase1
CGI-58, at least in vitro, also acts as an acyl-CoA-dependent acylglycerol-3-phosphate acyltransferase. (imingo.net)
Palmitoyl-CoA hydrolase (EC 3.1.2.2) is an enzyme in the family of hydrolases that specifically acts on thioester bonds. (wikipedia.org)
Activity1
Recent works have shown an inhibitory effect of long chain acyl-CoAs, such as palmitoyl-CoA and oleoyl-CoA, on ATGL activity. (imingo.net)
Domain2
The presence of a conserved acyl-CoA-binding domain in each ACBP enables it to bind acyl-CoA esters. (aocs.org)
ACBP3 lacks both ankyrin repeats and kelch motifs but it is larger (39.3 kDa) than ACBP6 (10.4 kDa), which is the smallest member and consists of only the acyl-CoA-binding domain. (aocs.org)