• α2M and clusterin are molecular chaperones involved in extracellular proteostasis, but α2M has a greater affinity for aggregation-prone proteins. (eurekalert.org)
  • Immunohistochemistry revealed a marked increase in extracellular clusterin protein expression in the perilesional cortex and ipsilateral hippocampus (7d to 1 month post-TBI), and ipsilateral thalamus (14d to 12 months post-TBI). (nature.com)
  • The study found that a particular extracellular matrix protein called clusterin, which is overproduced from the fat cells of obese patients, is strongly related to insulin resistance. (medicalxpress.com)
  • The clusterin protein is a key component of the extracellular matrix , so this work opens up a new vista to understand the role of clusterin in inter-cellular crosstalk of tissue microenvironments of various diseases, including diabetes , cancer and neurodegeneration," said the co-corresponding author, Dr. Stephen T. C. Wong, professor and chair of the Department of Systems Medicine and Bioengineering at Houston Methodist. (medicalxpress.com)
  • Moreover, it was proposed that Clusterin functions as an extracellular chaperon that stabilizes stressed proteins in a folding-competent state, and that the protein is involved in programmed cell death. (biovendor.com)
  • Prominent amongst such extracellular chaperones is clusterin (CLU), which is present in both plasma and cerebrospinal fluid (CSF). (biomedcentral.com)
  • The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model. (synergy-munich.de)
  • Here we show in a cellular model system and in neurons that Clusterin, an abundant extracellular chaperone, strongly enhances Tau aggregate seeding. (synergy-munich.de)
  • Transfection of ASC-17D Sertoli cells with a 20-base antisense oligonucleotide against clusterin mRNA resulted in extracellular release of LDH and DNA fragmentation. (korea.ac.kr)
  • Our findings suggest that plasma clusterin is a candidate biomarker for acute TBI diagnosis. (nature.com)
  • Clusterin may be a biomarker of disease, as well as a therapeutic target to potentially prevent this disease. (medicalxpress.com)
  • Glycosylation of Human Plasma Clusterin Yields a Novel Candidate Biomarker of Alzheimer's Disease. (ox.ac.uk)
  • Clusterin is a highly conserved glycoprotein ubiquitously expressed in the brain as well as in peripheral tissues in different species, including rodents and humans 15 . (nature.com)
  • Clusterin, aka apolipoprotein J, is a glycoprotein that can be found in most fluids. (ampersandbio.com)
  • Clusterin (CLU) is a secreted heterodimeric glycoprotein expressed in all organism fluids as well as in the intracellular matrix that plays key roles in several pathological processes. (torvergata.it)
  • BACKGROUND: Clusterin/apolipoprotein J (CLU) is a ubiquitous expressed glycoprotein with cytoprotective properties capable to prevent myocardial injury in experimental studies. (kl.ac.at)
  • Clusterin can be a multifunctional glycoprotein which can be widely distributed in lots of tissues and controlled by a number of environmental adjustments. (biosemiotics2013.org)
  • Clusterin is, in its major form, a secreted heterodimeric disulfide-linked glycoprotein (sCLU), which plays important roles in cell survival and death. (biomedcentral.com)
  • Clusterin is, in its major form, a secreted heterodimeric disulfide-linked glycoprotein (sCLU). (biomedcentral.com)
  • Clusterin is a glycoprotein that was initially isolated from the male reproductive system. (washington.edu)
  • Clusterin (apolipoprotein J) is a 75-80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. (wikipedia.org)
  • Two independent genome-wide association studies found a statistical association between a SNP within the clusterin gene and the risk of having Alzheimer's disease. (wikipedia.org)
  • Further studies have suggested that people who already have Alzheimer's disease have more clusterin in their blood, and that clusterin levels in blood correlate with faster cognitive decline in individuals with Alzheimer's disease, but have not found that clusterin levels predicted the onset of Alzheimer's disease. (wikipedia.org)
  • Previously this protein clusterin has been mostly studied for its role in neurodegenerative diseases such as Alzheimer's but it now appears to have a broader role in human physiology and disease. (medicalxpress.com)
  • Another defining prominent function of Clusterin is its induction in many severe physiological disturbances states including kidney degenerative diseases, prostate and vesicle carcinogenesis, ovarian cancer and several neurodegenerative conditions (Alzheimer's disease). (biovendor.com)
  • Clusterin is multifunctional with large amounts found in the central nervous system and has been associated with Alzheimer's disease. (ampersandbio.com)
  • Eight β64N glycoforms are significantly reduced in patients with high atrophy compared with those with low atrophy, which demonstrates the utility of clusterin isoforms as diagnostic and prognostic Alzheimer's disease (AD) markers. (ox.ac.uk)
  • Our previous mRNA-seq data revealed a 1.8-2.5 fold increase in clusterin mRNA expression in lesioned brain areas in rats with lateral fluid-percussion injury (FPI)-induced TBI. (nature.com)
  • Treatment of antisense oligonucleotide induced a marked reduction of synthesis for clusterin protein, but not for clusterin mRNA expression, suggesting the translational suppression of clusterin by antisense oligonucleotide. (korea.ac.kr)
  • Background Clusterin (Clu) is a stress-responding proteins with multiple biological features. (biosemiotics2013.org)
  • Further translational research involving mouse models is needed to learn more about how clusterin impacts each of the CMS components and whether administrating clusterin-inhibiting antibodies inhibits CMS. (medicalxpress.com)
  • We have shown that clusterin directly interacts with TDP-43 in vitro and potently inhibits its aggregation, and observed that in ER stressed neuronal cells, clusterin co-localized with TDP-43 and specifically reduced the numbers of cytoplasmic inclusions. (biomedcentral.com)
  • RayBio® Human Immunoquantitative (PCR-Based) Clusterin-like protein 1 ELISA Kit for cell culture supernatants, plasma, and serum samples. (raybiotech.com)
  • abstract = "Clusterin (CLU) has been noted to mark synovium adjacent to tenosynovial tumors, and studies suggest that podoplanin (PP) is upregulated in inflammatory arthritis. (johnshopkins.edu)
  • These results provide a novel and robust workflow suitable for rapid verification of specific clusterin glycoforms with utility as AD biomarkers. (ox.ac.uk)
  • In humans, clusterin is encoded by the CLU gene on chromosome 8. (wikipedia.org)
  • Due to its wide breath of tissue distribution many diverse physiological functions have been attributed to Clusterin including sperm maturation, membrane recycling, lipid transportation, tissue remodelling, complement inhibition and cell-cell or cell-substratum interactions. (biovendor.com)
  • Clusterin has been known to play important roles in cell-cell and/or cell-substratum interactions. (korea.ac.kr)
  • Riaz MA, Stammler A, Borgers M, Konrad L. Clusterin signals via ApoER2/VLDLR and induces meiosis of male germ cells. (biovendor.com)
  • From these results, we speculate that down-regulation of clusterin expression in the anchorage-dependent Sertoli cells prevents them from attaching to the plate, and therefore induces cell death. (korea.ac.kr)
  • We further showed that the expression of TDP-43 in transgenic Drosophila neurons induced ER stress and that co-expression of clusterin resulted in a dramatic clearance of mislocalized TDP-43 from motor neuron axons, partially rescued locomotor activity and significantly extended lifespan. (biomedcentral.com)
  • Our data strongly suggest that the signal transduction processes involved in induction of secretory clusterin (sCLU) expression after ionizing radiation (IR) exposure and during cellular replicative senescence are identical. (elsevierpure.com)
  • It is now widely accepted in the field that the normally secreted chaperone clusterin is redirected to the cytosol during endoplasmic reticulum (ER) stress, although the physiological function(s) of this physical relocation remain unknown. (biomedcentral.com)
  • Another interesting observation obtained in rat model suggests that measurement of urinary Clusterin levels may be a useful clinical marker for the severity of renal tubular damage. (biovendor.com)
  • Recent study demonstrates, that serum Clusterin level increases significantly in diabetic type II patients and in patients with developing coronary heart disease, or myocardial infarction. (biovendor.com)
  • These data raise the possibility that elevated Clusterin levels in serum may represent a strong indication of vascular damage. (biovendor.com)
  • In patients with systemic lupus erythematosus (SLE), reduced serum Clusterin levels correlated inversely with disease activity. (biovendor.com)
  • Kropáčková T, Šléglová O, Růžičková O, Vencovský J, Pavelka K, Šenolt L. Lower serum clusterin levels in patients with erosive hand osteoarthritis are associated with more pain. (biovendor.com)
  • The RayBio ® Custom Human Clusterin-like protein 1 IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human Clusterin-like protein 1 in serum, plasma, and cell culture supernatants. (raybiotech.com)
  • Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling. (alzforum.org)
  • We have examined in this study whether or not increased expression of clusterin is able to protect neuronal cells against intracellular protein aggregation and cytotoxicity, characteristics that are strongly implicated in a range of neurodegenerative diseases. (biomedcentral.com)
  • We also showed that in Drosophila photoreceptor cells, clusterin co-expression gave ER stress-dependent protection against proteotoxicity arising from both Huntingtin-Q128 and mutant (R406W) human tau. (biomedcentral.com)
  • We therefore conclude that increased expression of clusterin can provide an important defense against intracellular proteotoxicity under conditions that mimic specific features of neurodegenerative disease. (biomedcentral.com)
  • Recently we reported the transient expression of clusterin in pancreatic endocrine cells during the early developmental stages and suggested a role in aggregating the endocrine cells for islet formation. (korea.ac.kr)
  • The expression of clusterin was transiently increased as early as 2-8 h after plating the ASC-17D Sertoli cells to the culture flask, which was the period of cell attachment. (korea.ac.kr)
  • These findings suggested that interruption of cell to plate substratum interaction might lead to over-expression of clusterin from Sertoli cells to induce cell to cell aggregation or, perhaps, to re-establish attachment with the substratum. (korea.ac.kr)
  • We used the amyotrophic lateral sclerosis-associated protein TDP-43 as a primary model to investigate the effects of clusterin on protein aggregation and neurotoxicity in complementary in vitro, neuronal cell and Drosophila systems. (biomedcentral.com)
  • Tau/Clusterin complexes enter recipient cells via endocytosis and compromise the endolysosomal compartment, allowing transfer to the cytosol where they propagate aggregation of endogenous Tau. (synergy-munich.de)
  • Upon interaction with Tau aggregates, Clusterin stabilizes highly potent, soluble seed species. (synergy-munich.de)
  • Talmon, G , Wake, L & Muirhead, D 2013, ' Podoplanin and clusterin are reliable markers of nonneoplastic synovium at various sites ', International journal of surgical pathology , vol. 21, no. 6, pp. 587-590. (johnshopkins.edu)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clusterin (CLU) were tested 20 times on one plate, respectively. (biomatik.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clusterin (CLU) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
  • This assay has high sensitivity and excellent specificity for detection of Clusterin (CLU). (biomatik.com)
  • This assay employs an antibody specific for Human Clusterin-like protein 1 coated on a 96-well PCR plate. (raybiotech.com)
  • On this basis, we hypothesized that TBI leads to increases in the brain levels of clusterin protein, and consequently, increased plasma clusterin levels. (nature.com)
  • The glycosylation of clusterin from human plasma was comprehensively analyzed and characterized using mass spectrometry (MS)-based glycoproteomics analysis. (ox.ac.uk)
  • Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we have determined the differences in the glycoforms associated at each of the different glycosylation sites in plasma clusterin obtained from subjects of low hippocampal atrophy (n = 13) and high hippocampal atrophy (n = 14). (ox.ac.uk)
  • Clusterin can bind to aggregated LDL in 6H05 human being plasma and. (biosemiotics2013.org)
  • David Bradley et al, Clusterin Impairs Hepatic Insulin Sensitivity and Adipocyte Clusterin Associates With Cardiometabolic Risk, Diabetes Care (2019). (medicalxpress.com)
  • Subsequently, clusterin has been found to be widely distributed in a variety of tissues in mammals. (washington.edu)
  • Specific glycosylated peptides of clusterin are found associated with hippocampal atrophy. (ox.ac.uk)
  • In the present study, we have investigated the involvement of clusterin in cell-substratum interaction by the inhibition of clusterin synthesis using antisense oligonucleotide. (korea.ac.kr)
  • Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and erythrocytes, hence its name. (wikipedia.org)
  • Fat cells increase clusterin production as they enlarge in obesity. (medicalxpress.com)
  • In addition, up-regulation of clusterin mRNA was so much greater when the Sertoli cells were plated on the petri dish for the bacterial culture instead of in a animal cell culture flask that therefore, the cells failed to attach to it. (korea.ac.kr)
  • Lowered Clusterin levels could be involved in the pathogeneses of SLE on account of decreased protective effects. (biovendor.com)
  • Sertoli cell death by antisense oligonucleotide of clusterin was sequence specific and dose dependent. (korea.ac.kr)
  • The microarray data also indicated that clusterin (Clu) was up-regulated in MSCGATA-4. (biosemiotics2013.org)
  • Thus, upregulation of Clusterin, as observed in AD patients, may enhance Tau seeding and possibly accelerate the spreading of Tau pathology. (synergy-munich.de)
  • During a study spanning nearly a decade, researchers at The Ohio State University College of Medicine, Houston Methodist Research Institute and Houston Methodist Cancer Center have linked the protein clusterin-for the first time-to many different facets of cardiometabolic syndrome risk through its actions in the liver. (medicalxpress.com)
  • Standards and samples are pipetted into the wells and Clusterin-like protein 1 present in a sample is bound to the wells by the immobilized antibody. (raybiotech.com)
  • 3) p-Akt manifestation in MSCs was upregulated pursuing pre-treatment with clusterin without change altogether Akt. (biosemiotics2013.org)
  • Evidence is presented herein supporting the potential of the natural homeostatic glycopro-tein CLU (clusterin) as a novel therapeutic for the treatment of dry eye. (edu.au)
  • Furthermore ΔΨm was caspase and maintained 3/7 activity was reduced by 6H05 clusterin inside a concentration-dependent way. (biosemiotics2013.org)
  • This collaborative research is shedding new light on the importance of clusterin on "cardiometabolic syndrome," which may eventually lead to developing new treatments for this life-threatening combination of diabetes, high blood pressure and obesity," said Dr. K. Craig Kent, dean of the Ohio State College of Medicine. (medicalxpress.com)