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  • genes
  • Cloning is commonly used to amplify DNA fragments containing whole genes , but it can also be used to amplify any DNA sequence such as promoters , non-coding sequences and randomly fragmented DNA. (readtiger.com)
  • phenotype
  • Occasionally, the term cloning is misleadingly used to refer to the identification of the chromosomal location of a gene associated with a particular phenotype of interest, such as in positional cloning . (readtiger.com)
  • cells
  • Modern cloning vectors include selectable antibiotic resistance markers, which allow only cells in which the vector has been transfected, to grow. (readtiger.com)
  • 2000
  • Yet in August 2000, it recommended that the law be amended to allow the first stages of embryo cloning and related research to go ahead legally. (thecornerhouse.org.uk)
  • Yet in August 2000, it recommended that the law be amended to allow the first stages of embryo cloning and related research to go ahead legally, subject to a vote by Members of Parliament which is expected within the next few months. (thecornerhouse.org.uk)
  • create
  • A panel of experts led by Dr. Liam Donaldson, the nation's chief medical officer, has agreed to recommend changes to the law to allow the use of cloned embryos to create tissue to treat the sick. (washingtontimes.com)
  • They want to emphasize the difference between the cloning used to create Dolly the sheep and using embryos for tissue engineering. (washingtontimes.com)
  • Our intention is not to create cloned human beings but rather to make lifesaving therapies for a wide range of human disease conditions, including diabetes, strokes, cancer, Aids, and neurodegenerative disorders such as Parkinson's and Alzheimer's disease. (ffxii.net)
  • The resulting embryos were then used as a source of stem cells, which can be used to create specialised tissue cells for transplant operations. (abc.net.au)
  • Dolly the S
  • The British government on Tuesday granted the creator of "Dolly the sheep" licence to clone human embryos for research into Motor Neurone Disease, which affects the nervous system. (rediff.com)
  • The study, published in the journal Cell , used cloning techniques like those used to produce Dolly the sheep in Scotland in 1996. (abc.net.au)
  • Cloning continues to fascinate the science world, particularly after Dolly the Sheep made headlines across the globe in 1996. (express.co.uk)
  • Wilmut
  • But instead of growing healthy human replacement tissue, Dr Wilmut proposes to deliberately clone embryos from patients who have MND in order to "study how the disease progresses in very close detail. (rediff.com)
  • tissue
  • Chromosomal instability of murine adipose tissue-derived mesenchymal stem cells in long-term culture and development of cloned embryos. (semanticscholar.org)
  • The only region in which chick nuclei appeared at significantly greater frequency than in control tissue was the dorsal thigh muscle, the region into which the cloned chick cells were placed originally. (biologists.org)
  • We obtained the extraembryonic tissue from embryos at day 35 of pregnancy and examined the protein expression profiles using two-dimensional electrophoresis (2-D) and Western blotting. (biomedcentral.com)
  • Genome
  • Genome reprogramming in early mouse embryos is associated with nuclear reorganization and particular features such as the peculiar distribution of centromeric and pericentric heterochromatin during the first developmental stage. (biomedcentral.com)
  • sperm
  • The egg is then artificially coaxed to divide and behave in a way similar to a standard embryo fertilised by a sperm. (newscientist.com)
  • Then, after a long search, they finally found the best way to stimulate each egg so that it would develop into an embryo without the need to be fertilized with sperm. (kwit.org)
  • early
  • Another aspect is that cloned animals have led to early deaths and abnormalities. (digitaljournal.com)
  • A blastula is an embryo in the early stage of development. (trend.az)
  • Together, the results suggest that proper organization of constitutive heterochromatin in early embryos is involved in the initial developmental steps and might have long term consequences, especially in cloning procedures. (biomedcentral.com)