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  • process
  • The term clone , invented by J. B. S. Haldane , is derived from the Ancient Greek word κλών klōn , "twig", referring to the process whereby a new plant can be created from a twig. (readtiger.com)
  • insert
  • Following ligation the vector with the insert of interest is transfected into cells. (readtiger.com)
  • Nevertheless, these selection steps do not absolutely guarantee that the DNA insert is present in the cells obtained. (readtiger.com)
  • successfully
  • As the aforementioned procedures are of particularly low efficiency, there is a need to identify the cells that have been successfully transfected with the vector construct containing the desired insertion sequence in the required orientation. (readtiger.com)
  • term
  • the final e came into use to indicate the vowel is a "long o" instead of a "short o". Since the term entered the popular lexicon in a more general context, the spelling clone has been used exclusively. (readtiger.com)
  • Occasionally, the term cloning is misleadingly used to refer to the identification of the chromosomal location of a gene associated with a particular phenotype of interest, such as in positional cloning . (readtiger.com)